Article
Biochemistry & Molecular Biology
Xin Ming, Zongyi Bo, Yurun Miao, Huan Chen, Chenyi Bao, Liumei Sun, Rui Xi, Qiuping Zhong, Pu Zhao, Yong-Sam Jung, Yingjuan Qian
Summary: The PRV infection activates ATM and DNA-PK signaling pathways, while the PRV-encoded kinase UL13 interacts with and phosphorylates H2AX. Deletion of UL13 attenuates PRV neuroinvasiveness and virulence in vivo. UL13-mediated H2AX phosphorylation plays a pivotal role in efficient PRV replication and progeny production.
Article
Virology
Xin Ming, Huan Chen, Ying Yang, Pu Zhao, Liumei Sun, Caisheng Zhang, Hyun-Jin Shin, Jeong-Soo Lee, Yong-Sam Jung, Yingjuan Qian
Summary: This study reveals that the porcine epidemic diarrhea virus (PEDV) induces DNA damage response (DDR) through the ROS-ATM and caspase7-CAD-gamma H2AX signaling pathways, which facilitates its early replication.
Article
Environmental Sciences
Ayumi Eguchi, Sayaka Mizukami, Misato Nakamura, Sousuke Masuda, Hirotada Murayama, Masashi Kawashima, Mari Inohana, Rei Nagahara, Mio Kobayashi, Risako Yamashita, Suzuka Uomoto, Emi Makino, Ryoichi Ohtsuka, Naofumi Takahashi, Shim-Mo Hayashi, Robert R. Maronpot, Makoto Shibutani, Toshinori Yoshida
Summary: The study suggests that MNZ may induce steatosis-related hepatocarcinogenesis in HFD-fed rats by inducing DNA double-strand breaks and modulating autophagy.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Editorial Material
Cell Biology
Jake Atkinson, Eva Bezak, Ivan Kempson
Summary: DNA double-strand breaks play a fundamental role in cell biology, but there is a lack of a reliable assay for quantitatively visualizing DSBs. Relying on proxy detection methods can lead to misinterpretation of conventional assays, highlighting the need for the development of more accurate DSB detection methods.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yan Zhang, Hao Gong, Ji-Shuai Wang, Meng-Na Li, De-Li Cao, Jun Gu, Lin-Xia Zhao, Xin-Dan Zhang, Yu-Tao Deng, Fu-Lu Dong, Yong-Jing Gao, Wen-Xing Sun, Bao-Chun Jiang
Summary: In neuropathic pain, the expression of ?H2AX and ATM is downregulated. Inhibiting ATM with KU55933 can reduce the level of ?H2AX and induce mechanical allodynia and thermal hyperalgesia. Further studies show that inhibiting ATM can upregulate ERK phosphorylation and downregulate potassium ion channel genes, increasing sensory neuron excitability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Weiyin Xu, Ping Yan, Ziyan Zhou, Jingting Yao, Haochun Pan, Luyao Jiang, Zongyi Bo, Bo Ni, Mingxia Sun, Song Gao, Changchao Huan
Summary: Pseudorabies virus (PRV) infection in swine can cause high morbidity and mortality, leading to significant economic losses. In this study, the role of histone deacetylase 6 (HDAC6) in PRV infection was investigated. The inhibition of HDAC6 significantly decreased PRV replication, while its overexpression promoted PRV replication. PRV infection induced DNA damage response (DDR), and HDAC6 inhibition and knockout decreased DDR, suggesting that HDAC6 may be a crucial factor in promoting PRV replication.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Ievgeniia Gazo, Roman Franek, Radek Sindelka, Ievgen Lebeda, Sahana Shivaramu, Martin Psenicka, Christoph Steinbach
Summary: Sterlet embryos show varying sensitivity to different genotoxicants, with some inducing DNA damage and activating DDR pathways, while others causing DNA damage without activating known DDR pathways, with effects also being stage-specific in embryo development. This study provides foundational information for future investigation of sterlet DDR pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Lukas Goering, Sarah Schumann, Jessica Muller, Andreas K. Buck, Matthias Port, Michael Lassmann, Harry Scherthan, Uta Eberlein
Summary: This study investigated the induction and repair of DSB damage in peripheral blood mononuclear cells following internal and external irradiation. The results suggest that the induction and repair of DSB damage depend on the absorbed dose, with similar repair rates as low linear energy transfer irradiation.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
S. Schumann, H. Scherthan, K. Pfestroff, S. Schoof, A. Pfestroff, P. Hartrampf, N. Hasenauer, A. K. Buck, M. Luster, M. Port, M. Lassmann, U. Eberlein
Summary: This study provides novel data on ex vivo DSB repair in internally irradiated PBMCs of patients before radionuclide therapy, showing efficient repair occurs after internal irradiation with 50 mGy absorbed dose, and that the induction and repair rate after I-131 exposure is comparable to that of external irradiation with gamma- or X-rays.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Article
Biochemistry & Molecular Biology
Xiuyan Ding, Weifeng Yuan, Hao Yang, Chang Liu, Shitao Li, Liqian Zhu
Summary: The study demonstrates that BoHV-1 infection disrupts DNA damage repair mediated by 53BP1 and suggests that β-catenin plays a role in restricting both virus replication and DNA damage in A549 cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yong Jung Kang, Jung Yoon Jang, Young Hoon Kwon, Jun Ho Lee, Sanggwon Lee, Yujin Park, Young-Suk Jung, Eunok Im, Hyung Ryong Moon, Hae Young Chung, Nam Deuk Kim
Summary: MHY2245 induces cell cycle arrest through DNA damage-induced p53 activation and apoptosis via caspase activation, showing anticancer effects associated with DNA damage response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sayanthooran Saravanabavan, Gopala K. Rangan
Summary: Increased DNA damage response signaling in kidney cyst-lining epithelial cells (CECs) may be a target for therapy in ADPKD. However, inhibiting ATM kinase and low-dose cisplatin together does not selectively induce cell death in CECs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Sheema Almozyan, James Coulton, Roya Babaei-Jadidi, Abdolrahman S. Nateri
Summary: Recent studies have indicated that FLYWCH1 may play a critical role in facilitating the recruitment of DNA-damage response proteins in the context of DNA damage and repair.
Article
Biochemistry & Molecular Biology
Ifeoma Julieth Dikeocha, Abdelkodose Mohammed Al-Kabsi, Hsien-Tai Chiu, Mohammed Abdullah Alshawsh
Summary: This study demonstrated the potential role of F. prausnitzii in the prevention and management of colorectal cancer, showing its anti-tumorigenic and antiproliferative effects, as well as its modulation of gut microbiota diversity in rats.
Article
Biochemical Research Methods
Ajit K. Sharma, Amira Mohammed Fitieh, Jana Yasser Hafez Ali, Ismail Hassan Ismail
Summary: DNA end resection is a critical step in repairing DNA double-strand breaks (DSBs) through homologous recombination. This article describes a quantitative polymerase-chain-reaction-based method to measure the formation of single-stranded DNA (ssDNA) intermediates during DNA end resection. The ER-AsiSI system is used to digest unresected double-stranded DNA at DSB sites using restriction endonucleases with differential digestion patterns.