Article
Environmental Sciences
Chris Daniel Philus, Biswanath Mahanty
Summary: The microbial toxicity of test substances in tetrazolium assay is often quantified using IC50 values, but the interpretation of these estimates is limited and can vary depending on test conditions. A factorial design-based end-point microbial toxicity assay was conducted in this study, revealing a significant interaction between inoculum and tetrazolium dose on formazan production. A dynamic model framework was then used to capture nonlinearities in microbial growth, glucose uptake, and formazan production, providing a stable toxicity index for test substances and insight into their toxic effects.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Laura Kallay, Vaibhavkumar S. Gawali, Donatien Kamdem Toukam, Debanjan Bhattacharya, Andrew Jenkins, Soma Sengupta, Daniel A. Pomeranz Krummel
Summary: Ion channels play critical roles in cell development and homeostasis. Cancer cells utilize ion channels to drive their own development and adapt to the microenvironment. Targeting ion channels pharmacologically may be a promising approach for treating solid malignancies.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Biochemistry & Molecular Biology
Bo Hee Choi, Mi-Ri Kim, Yu Na Jung, Smee Kang, Jungil Hong
Summary: The effects of porphyrin-related compounds on the color response of the MTT tetrazolium-based assay were investigated. It was found that certain porphyrin compounds, namely ZnPP, PPIX, and ZnPC, significantly reduced the color response without affecting cell viability. The compounds also rapidly degraded the already-produced MTT formazan under light, demonstrating their photosensitizing properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Yeong Ji Oh, Jungil Hong
Summary: The MTT assay was used to analyze the growth of E. coli and L. mesenteroides, with 1 N NaOH identified as the most suitable solvent for analyzing bacterial growth in the MTT assay.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Ecology
Jinglie Zhou, Susanna M. Theroux, Clifton P. Bueno de Mesquita, Wyatt H. Hartman, Ye Tian, Susannah G. Tringe
Summary: The study showed that wetland restoration effectively restored microbial communities in former industrial salt ponds to compositions more similar to reference wetlands, reducing salinity, sulfate concentrations, and methane emissions.
Article
Biotechnology & Applied Microbiology
Cleide O. de A. Moller, Josue L. Castro-Mejia, Lukasz Krych, Fergal P. Rattray
Summary: The study found that adding histamine-producing bacteria to reduced-salt Cheddar cheese led to higher histamine levels, while higher salt content can slow down the growth rate of non-starter microbiota. There was a correlation between increased histamine levels, non-starter microbiota development, and pH.
Article
Chemistry, Physical
Peijun Zhu, Jiahao Chen, Ping Li, Shulan Xu
Summary: The CCK-8 assay is not suitable for evaluating the cytotoxicity of biodegradable Zn-based metals in direct contact tests, as zinc and pre-treated zinc can directly convert tetrazolium salts to formazan, leading to inaccurate results.
Article
Multidisciplinary Sciences
Fariba Abbasi, Hassan Hashemi, Mohammad Reza Samaei, Amir SavarDashtaki, Abooalfazl Azhdarpoor, Mohammad Javad Fallahi
Summary: The MTT assay is commonly used for determining cell toxicity, but the interference effect of SiO2 nanoparticles and wavelength has a synergistic effect on this method. The concentration of SiO2 nanoparticles is a confounding factor in colorimetric for MTT assay.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Ryan K. Muir, Matteo Guerra, Matthew M. Bogyo
Summary: This review highlights recent methodologies employed in activity-based diagnostics for detecting various diseases by leveraging the dysregulated enzyme expression and activity. These methods exhibit exquisite signal sensitivity and specificity in complex biological systems, and some have advanced into clinical trials.
ACS CHEMICAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yaru Gao, Hong Zou, Baoshan Wang, Fang Yuan
Summary: Saline soils pose a significant challenge to agriculture, and there is an increasing need to find effective solutions to improve plant salt tolerance. This review article explores the potential of using plant growth-promoting bacteria, both endosymbiotic and exosymbiotic, to enhance plant growth and alleviate salt stress in saline soils. The mechanisms and applications of these bacteria are discussed to provide a theoretical basis for their future use in agricultural systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Aashini Rajpal, Lucie Huart, Christophe Nicolas, Corinne Chevallard, Jean-Michel Guigner, Paulo Dasilva, Pascal Mercere, Benoit Gervais, Marie-Anne Herve du Penhoat, Jean-Philippe Renault
Summary: Soft X-rays deposit a large amount of energy in the nanometric range, resulting in the formation of doubly ionized water molecules and the emission of secondary electrons. This study focuses on detecting and quantifying the production of superoxide via direct pathway reactions. Experimental results show that the yield of HO2 degrees via this pathway is 0.005 (+/- 0.0007) mu mol/J. Additionally, experiments were conducted to determine the production of HO2 degrees via an indirect pathway involving solvated electrons, which exhibited a steep decrease at around 1280 eV and a minimum close to zero at 800 eV.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Review
Food Science & Technology
Cuixia Sun, Xuelian Zhou, Zining Hu, Wei Lu, Yiguo Zhao, Yapeng Fang
Summary: Salt plays a crucial role in food processing and consumption, but high intake can pose health risks. Consumer demand for healthier options drives the research and development of reduced-salt products, which faces challenges due to the impact of salt on food characteristics.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2021)
Article
Microbiology
Leila Satari, Alba Guillen, Adriel Latorre-Perez, Manuel Porcar
Summary: The study revealed that salts from marine origins are dominated by Archaea, whereas salts from other sources or salt supplemented with ingredients are dominated by bacteria.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Yingying Hu, Yongjie Li, Jiamin Zhu, Baohua Kong, Qian Liu, Qian Chen
Summary: The study found that inoculation of lactic acid bacteria can increase the moisture content, decrease pH values, and increase total free amino acid content in reduced-salt dry sausage. Weissella hellenica and Lactobacillus plantarum were shown to have the potential to improve taste profiles.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Agata Koziol, Katarzyna Macegoniuk, Ewa Grela, Agnieszka Grabowiecka, Monika Biernat, Stanislaw Lochynski
MOLECULAR BIOLOGY REPORTS
(2019)
Article
Chemistry, Applied
Agata Koziol, Ewa Grela, Katarzyna Macegoniuk, Agnieszka Grabowiecka, Stanislaw Lochynski
NATURAL PRODUCT RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Joanna Kozlowska, Ewa Grela, Dagmara Baczynska, Agnieszka Grabowiecka, Miroslaw Aniol
Article
Biochemistry & Molecular Biology
Joanna Kozlowska, Bartlomiej Potaniec, Dagmara Baczynska, Barbara Zarowska, Miroslaw Aniol
Article
Biochemistry & Molecular Biology
Katarzyna Stec, Joanna Kozlowska, Anna Wroblewska-Kurdyk, Bozena Kordan, Miroslaw Aniol, Beata Gabrys
Article
Biochemistry & Molecular Biology
Anna Duda-Madej, Joanna Kozlowska, Pawel Krzyzek, Miroslaw Aniol, Alicja Seniuk, Katarzyna Jermakow, Ewa Dworniczek
Article
Chemistry, Physical
Joanna Kozlowska, Bartlomiej Potaniec, Miroslaw Aniol
Article
Chemistry, Medicinal
Aikaterini Pagoni, Agnieszka Grabowiecka, Wojciech Tabor, Artur Mucha, Stamatia Vassiliou, Lukasz Berlicki
Summary: A new class of bifunctional inhibitors of bacterial ureases was developed in this study, showing complex structure-dependent kinetics of inhibition. One compound in particular exhibited remarkably reactive inhibition against a specific urease enzyme. The compounds also demonstrated potential in whole-cell inhibition assays, with some showing slow-binding and reversible kinetics.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Chemistry, Inorganic & Nuclear
Stamatia Vassiliou, Aikaterini Pagoni, Ewelina Weglarz-Tomczak, Michal Talma, Wojciech Tabor, Agnieszka Grabowiecka, Lukasz Berlicki, Artur Mucha
Summary: The phosphinic acid functionality is a valuable scaffold for constructing biologically active compounds, particularly enzyme inhibitors. This article presents two recent achievements in using phosphinic acids as ligands for hydrolases, including the synthesis of a dipeptide analog inhibitor of metalloaminopeptidases and a catechol-based inhibitor of urease, as well as discussing the activity and mode of binding of phosphinic compounds to enzymes.
PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS
(2022)
Article
Chemistry, Physical
Beata Biernacka, Dariusz Dziki, Joanna Kozlowska, Iwona Kowalska, Agata Soluch
Summary: This study found that the green shaft of leek contains more nutrients and higher antioxidant capacity compared to the white shaft, with the dominant phenolic acids being ferulic acid and protocatechuic acids. Additionally, higher drying temperature led to the degradation of phenolic compounds and reduced antioxidant properties of leek shafts.
Article
Chemistry, Medicinal
Katarzyna Macegoniuk, Wojciech Tabor, Luca Mazzei, Michele Cianci, Miroslaw Giurg, Kamila Olech, Malgorzata Burda-Grabowska, Rafal Kaleta, Agnieszka Grabowiecka, Artur Mucha, Stefano Ciurli, Lukasz Berlicki
Summary: Screening of 25 analogs of Ebselen led to the discovery of highly potent inhibitors of Sporosarcina pasteurii urease, with a Ki value in a low picomolar range. The exceptional activity was attributed to the presence of a dihalogenated phenyl ring, which increased pi-pi and pi-cation interactions. Biological studies showed good potency for monosubstituted N-phenyl derivatives in inhibiting ureolysis in Proteus mirabilis cells.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Review
Infectious Diseases
Anna Duda-Madej, Jakub Stecko, Jakub Sobieraj, Natalia Szymanska, Joanna Kozlowska
Summary: This review discusses the antimicrobial activity of naringenin and its derivatives as a phytobiotic against antibiotic-resistant bacteria and fungi. With the increase in antibiotic resistance, it has become a global health problem. The potential molecular mechanisms of action of naringenin are also described. The review emphasizes the importance of using plant-derived substances to prevent and treat infections in the era of drug resistance.
Article
Cell Biology
Abril Alondra Barrientos-Bonilla, Paola Belem Pensado-Guevara, Abraham Puga-Olguin, Rasajna Nadella, Aurora del Carmen Sanchez-Garcia, Laura Mireya Zavala-Flores, Arnulfo Villanueva-Olivo, Iliana Tamara Cibrian-Llanderal, Maria de Jesus Rovirosa-Hernandez, Daniel Hernandez-Baltazar
Summary: This study evaluated the impact of BrdU on liver function and found that multiple injections of BrdU did not induce hepatocellular damage.
Article
Cell Biology
Pan Zhang, Qun Feng, Wenxiao Chen, Xizhuang Bai
Summary: It was found that catalpol antagonizes LPS-mediated inflammation and suppressive osteoblast differentiation by inhibiting inflammation and promoting osteoblast differentiation. This finding contributes to a better understanding of the pathogenesis of osteoporosis.
