Article
Agriculture, Dairy & Animal Science
Yao Zhang, Jiayun Hu, Jiaxin Wang, Chen Liu, Xiaofeng Liu, Juan Sun, Xinjie Song, Yuanfeng Wu
Summary: A novel traditional MCE substitute was produced from Bacillus velezensis DB219 with high activity and purification yield. It showed the highest milk-clotting activity at weak acid condition, particularly at pH 5.5 with the addition of CaCl2. DB219 MCE preferred hydrolyzing β-casein and had weaker hydrolytic activity towards α-casein.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Yang Liu, Zufang Wu, Xiaoxiong Zeng, Peifang Weng, Xin Zhang, Changyu Wang
Summary: BsGal1332 is a cold-adapted enzyme with strict substrate specificity for galactosyl compounds, making it suitable for efficient lactose hydrolysis in dairy products.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agriculture, Dairy & Animal Science
Na Li, Yang Liu, Changyu Wang, Peifang Weng, Zufang Wu, Yazhu Zhu
Summary: The novel galactosidase Gal3149 from Bacillus velezensis SW5 showed higher enzyme activity for oNPG than pNPαG, optimal activity at pH 8.0 and 50°C, and good thermal stability. It was also significantly improved by K+ and Na+, but inhibited by several substances. Gal3149 has potential applications in lactose-reduced dairy industry.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jing Lu, Yu Zhao, Rong Hu, Yu Cheng, Junhuan Qin, Jie Yang, Yaowei Fang, Mingsheng Lyu, Shujun Wang
Summary: In this study, a protease-producing B. velezensis strain was screened and the production and reaction conditions of the protease were optimized. The protease exhibited good antioxidant function in the enzymolysis of mussel meat.
Article
Horticulture
Bao Wang, Erfeng Li, Yang Lin, Tongbin Xiao, Xuncong Ji, Zhixiang Zhao, Wanrong Yan
Summary: Bacillus velezensis Yb-1 strain has the potential to effectively control Colletotrichum capsici and Botrytis cinerea, and promote the growth of peppers.
Article
Environmental Sciences
Chonlong Chio, Sarita Shrestha, Griffin Carr, Janak Raj Khatiwada, Yuen Zhu, Ou Li, Xuantong Chen, Jing Hu, Wensheng Qin
Summary: Bioproduction is a promising approach to obtain useful and green chemicals. However, the high purification cost of biomolecules in the downstream process has been a major challenge for scaling up the application of bioproducts. In this study, a new strain of Bacillus velezensis was isolated and optimized for the production of amylase. The enzymes and biosurfactant were then purified using sequential ammonium sulfate precipitation, which showed a higher yield compared to traditional methods. The purified biosurfactant exhibited higher emulsion activity and petroleum hydrocarbon removal rate than conventional surfactants. This study provides a new approach for purifying bioactive compounds and explores the potential of purified biosurfactant in bioremediation.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Agricultural Engineering
Qiao Meng, Chenghui Lu, Hua Gao, Guoxuan Chen, Lingtian Wu, Jinnan Wu, Sha Li, Bing-Fang He
Summary: This study focused on the non-grain biorefining of Jerusalem artichoke for efficient exopolysaccharide production using Bacillus velezensis LT-2. Results showed that LT-2 could directly utilize Jerusalem artichoke tuber powder for EPS production, with an EPS yield of 11.47 +/- 0.33 g/L in the simultaneous saccharification and fermentation mode. Transcriptome sequencing results indicated that inulin accelerated nucleotide-sugars biosynthesis, induced EPS synthetic gene cluster transcription, and strengthened various metabolic pathways to ensure EPS efficient synthesis.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jaeyoung Choi, Jiyoung Nam, Moon-Hyeong Seo
Summary: Bacillus spp. are important in producing bioactive natural products with agricultural and medical applications, especially the lipopeptides fengycin, iturin, and surfactin with antagonistic activity against other microbes. A novel strain NST6 identified as B. velezensis has 21 putative biosynthetic gene clusters, including those responsible for bacillomycin and surfactin. Analysis shows that gene clusters for iturin families are well conserved in B. amyloliquefaciens species, while clusters for fengycin and surfactin have dynamic distribution at gene level.
Article
Plant Sciences
Zhuoran Li, Jianan Hu, Qi Sun, Xi Zhang, Ruokui Chang, Yuanhong Wang
Summary: The BvEP protein derived from Bacillus velezensis LJ02 was found to enhance the resistance of tomato plants to B. cinerea. This protein induced a hypersensitivity response in tobacco plants and upregulated the expression of several defense genes in transgenic tomato plants. Moreover, transient overexpression of BvEP reduced the rotting rate and lesion diameter of tomato fruits infected by B. cinerea. This study provides new insights into innovative breeding of tomato disease resistance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Food Science & Technology
Yihui Wang, Tariq Aziz, Gege Hu, Jing Liu, Zhang Min, Yang Zhennai, Metab Alharbi, Abdulrahman Alshammari, Abdullah F. Alasmari
Summary: Bacillus velezensis YH-1 isolated from rice wine starter was found to produce a milk-clotting enzyme (MCE) with remarkable activity. The MCE was identified as a Zn2+ metalloproteinase and showed superior performance compared to commercial rennet in cheese production.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Microbiology
Zhongmin Tang, Xiaojie Cao, Huili Zhang
Summary: This study compared the dynamic parameters of solid-state fermentation (SSF) and submerged fermentation (SMF) of Bacillus velezensis ND and proposed a method for producing the biosurfactant iturin A using SSF. Iturin A has broad-spectrum antifungal activity and the highest yield among previous studies. Bacillus velezensis ND also possesses various biological functions and the ability to control plant pathogens. In cotton pot experiments, it promotes cotton growth and reduces Verticillium wilt.
JOURNAL OF BASIC MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yao Zhang, Jiayun Hu, Xiaofeng Liu, Chunmin Jiang, Juan Sun, Xinjie Song, Yuanfeng Wu
Summary: This study aimed to isolate and identify a strain with high milk-clotting activity and low proteolytic activity, and optimize the fermentation conditions for maximum milk-clotting enzyme production. A strain of Bacillus velezensis DB219 with high activity was isolated and identified. The optimal fermentation conditions were determined through experiments. DB219 MCE production was significantly improved.
Article
Entomology
Danvir Rajesh Ramesar, Charles Haig Hunter
Summary: Bacillus sp. PHP1601, a member of B. subtilissensu lato, has the ability to antagonize fly larvae, especially Lucilia cuprina. The larvicidal response is attributed to the bioactive lipopeptide compounds in the partially purified biosurfactant extracts. Based on the analysis of multiple-locus sequence, PHP1601 is confirmed to be a strain of B. velezensis.
Article
Agriculture, Multidisciplinary
Ziqiang Yuan, Bo Zhou, Xiuna Wu, Li Wang, Guiling Li, Jingwen Liu, Qianjin Kang, Daren Wu, Jian Li
Summary: This study isolated and purified a novel antifungal compound, Baelezcin A, from the endophytic bacterium Bacillus velezensis SJ100083. Baelezcin A effectively suppressed gray mold on cherries by inducing reactive oxygen species accumulation in spores and leakage of mycelium cytoplasmal contents, resulting in reduced spore germination. Moreover, it was proven to be biologically safe within a certain range.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Food Science & Technology
Hong Liu, Shen Luo, Jun Liu, Qiaojuan Yan, Shaoqing Yang, Zhengqiang Jiang
Summary: Green soybean shuidouchi, fermented by Bacillus velezensis, showed multiple bioactivities including anticoagulant, fibrinolytic, and antioxidant activities with optimized processing conditions. It contained higher levels of chemical components compared to unfermented shuidouchi, indicating its potential for further development of functional soybean products.
FOOD SCIENCE & NUTRITION
(2021)
