期刊
EXPERIMENTAL AND APPLIED ACAROLOGY
卷 67, 期 1, 页码 49-63出版社
SPRINGER
DOI: 10.1007/s10493-015-9930-3
关键词
Panonychus citri; Mitochondrial MnSOD; RT-qPCR; Expression pattern; Oxidative stress
类别
资金
- National Natural Science Foundation [31171851]
- Agro-scientific Research in the Public Interest [201103020]
- Innovative Research Team in Universities [IRT0976]
- earmarked fund for the Modern Agro-industry (Citrus) Technology Research System of China
- Fundamental Research Funds for the Central Universities of China [XDJK2013A017]
Superoxide dismutase (SOD) is a family of enzymes with multiple isoforms that possess antioxidative abilities in response to environmental stresses. Panonychus citri is one of the most important pest mites and has a global distribution. In this study, three distinct isoforms of SOD were cloned from P. citri and identified as cytoplasmic Cu-ZnSOD (PcSOD1), extracellular Cu-ZnSOD (PcSOD2), and mitochondrial MnSOD (PcSOD3). mRNA expression level analysis showed that all three isoforms were up-regulated significantly after exposure to the acaricide abamectin and to UV-B ultraviolet irradiation. In particular, PcSOD3 was up-regulated under almost all environmental stresses tested. The fold change of PcSOD3 expression was significantly higher than those of the two Cu-ZnSOD isoforms. Taken together, the results indicate that abamectin and UV-B can induce transcripts of all three SOD isoforms in P. citri. Furthermore, PcSOD3 seems to play a more important role in P. citri tolerance to oxidative stress.
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