期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 18, 页码 15442-15448出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b02626
关键词
tetrahedral DNA nanostructures (TDNs); cell; interaction; biocompatibility; cell cycle
资金
- National Key Research and Development Program [2016YFA0400902]
- Key Research Program of Frontier Sciences, CAS [QYZDJ-SSW-SLH031]
- Open Large Infrastructure Research of Chinese Academy of Sciences
- National Natural Science Foundation of China [11675251, 21390414, U1532119, U1432116, 21675167, 11575278, 21505148, 11705270]
- Instrument Developing Project of the Chinese Academy of Sciences
- Youth Innovation Promotion Association of CAS [2016236]
- China postdoctoral science foundation [2016M601679]
The advent of DNA technology has demonstrated great potential in a wide range of applications, especially in the field of biology and biomedicine. However, current understanding of the toxicological effects and cellular responses of DNA nanostructures remains to be improved. Here, we chose tetrahedral DNA nanostructures (TDNs), a type of nanocarriers for delivering molecular drugs, as a model for systematic live-cell analysis of the biocompatibility of TDNs to normal bronchial epithelial cells, carcinoma cells, and macrophage. We found that the interaction behaviors of TDNs in different cell lines were very different, whereas after internalization, most of the TDNs in diverse cell lines were positioned to lysosomes. By a systematic assessment of cell responses after TDN exposure to various cells, we demonstrate that internalized TDNs have good innate biocompatibility. Interestingly, we found that TDN-bearing cells would not affect the cell cycle progression and accompany cell division and that TDNs were separated equally into two daughter cells. This study improves our understanding of the interaction of DNA nanostructures with living systems and their biocompatibility, which will be helpful for further designing DNA nanostructures for biomedical applications.
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