期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 20, 页码 17100-17106出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b04287
关键词
carbon nanotube field-effect transistors; lupeol; lipopolysaccharides; tumor necrosis factor alpha; Raw 264.7 cells
资金
- Ministry of Science and ICT (MSIT) of Korea [2015R1A2A2A04002733, H-GUARD_2013M3A6B2078961]
- European Research Council (ERC) under the European Union [682286]
- National Research Foundation of Korea (NRF) - Korean government (The Ministry of Science and ICT) [NRF-2015R1A2A2A04002733]
- [2014M3A7B4051591]
- [2017R1A2B2006808]
Modified floating electrode-based sensors were developed to quantitatively monitor the levels of tumor necrosis factor alpha (TNF-alpha), a pro-inflammatory cytokine related with inflammatory bowel disease (IBD), and to evaluate the effect of drugs on the cytokine levels. Here, antibodies (anti-TNF-alpha) were immobilized on the floating electrodes of carbon nanotube devices, enabling selective and real-time detection of TNF-alpha among various cytokines linked to IBD. This sensor was able to measure the concentrations of TNF-alpha with a detection limit of 1 pg/L, allowing the quantitative estimation of TNF-alpha secretion from mouse macrophage Raw 264.7 cells stimulated by lipopolysaccharides (LPS). Notably, this method also allowed us to monitor the anti-inflammatory effect of a drug, lupeol, on the activation of the LPS-induced nuclear factor kappa B signaling in Raw 264.7 cells. These results indicate that our novel TNF sensor can be a versatile tool for biomedical research and clinical applications such as screening drug effects and monitoring inflammation levels.
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