Article
Chemistry, Analytical
Guodong Yao, Zunyu Xiao, Shan Yu, Ke Yao, Dahai Liu, Kexin Chen, Zhimin Wei, Yujun Li, Feifei Sun
Summary: miRNA plays a crucial role in cancer development, with challenges in accurate extraction from clinical samples. A novel fluorescence assay proposed utilizes DSN signal amplification for accurate analysis of miRNA, offering stable, sensitive, and label-free advantages.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Zhiguo Li, Junren Chen, Lingling Zhang, Yuge Liu, Yongmei Jia, Guohua Zhou, Liqiang Luo
Summary: In this article, a new signal amplification strategy based on signal on-off-off is proposed to improve the detection sensitivity of a breast cancer 1 (BRCA1) electrochemical sensor. The designed sensor demonstrates excellent detection sensitivity for BRCA1, capable of distinguishing single base mismatch BRCA1, two base mismatch BRCA1, and non-complementary DNA.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Chenxin Fang, Ping Ouyang, Yuxing Yang, Yang Qing, Jialun Han, Wenyan Shang, Yubing Chen, Jie Du
Summary: A microRNA detection platform utilizing a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system has been proposed for rapid and efficient detection of miRNA-21. This system achieves signal amplification and fluorescence intensity amplification through different mechanisms, showing broad prospects in nucleic acid analysis and detection.
Article
Biochemical Research Methods
Gang Zhao, Xiaohang Yan, Yi Zhang, Jing Deng, Xiaojun Liang
Summary: MicroRNA and circular RNA are promising biomarkers for early diagnosis of various diseases, but few methods can simultaneously and sensitively detect multiple MicroRNAs. In this study, the authors designed a novel approach to improve the detection sensitivity of MicroRNA and circular RNA.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Zhuoer Zeng, Rong Zhou, Ruowei Sun, Xun Zhang, Di Zhang, Qubo Zhu, Chuanpin Chen
Summary: In this study, we developed a novel hairpin-free NHCR-based flow cytometric immunoassay for detecting prostate specific antigen (PSA). By utilizing DNA self-assembly and fluorescence amplification, dendritic DNA nanostructures with fluorescent signal molecules were formed on magnetic beads (MBs), and flow cytometric analysis was employed for quantifying the target protein. Compared to commercially available PSA kits, this method showed higher sensitivity, selectivity, and the ability to eliminate background signal interference.
ANALYTICA CHIMICA ACTA
(2022)
Article
Engineering, Environmental
Guang Li, Xinyu Zhou, Shengjie Ge, Xiaotao Bao, Xiaoyan Liu, Aidong Sun
Summary: A novel lab-on-a-chip-based surface-enhanced Raman scattering (LoC-SERS) analysis system was developed with a target-triggered dual cycle amplification strategy for quantitative analysis of miRNAs. The system showed promising potential in clinical application for the prediction, diagnosis, monitoring, and staging of Laryngeal carcinoma (LC).
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Yuqing Zhu, Xiaohe Zheng, Shujun Zhou, Wenjing Xiao, Xiaorui Sun, Jianming Zhou, Feiyang Qian, Tenghua Zhang, Yan Sheng, Jiaming Hu
Summary: The article introduces a novel dual signal amplification strategy called CENTER for the ultrasensitive detection of miR-141, a potential marker for prostate cancer. By integrating CRISPR/Cas technology and other amplification methods, CENTER shows great promise in the field of miRNA detection.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Fenghui Qi, Yuelan He, Yanian Xiong, Jianzhong Lu, Yuhao Zhang
Summary: A simple and sensitive turn-off biosensor for miRNA-21 detection based on tyramine signal amplification (TSA) was developed, which can achieve high sensitivity detection of low concentration miRNA. The method has great potential for disease diagnosis, as demonstrated in human lung cancer cells.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Qingyu Wang, Haobo Sun, Dongxiao Wen, Lei Wang, Lianzhi Li, Jinming Kong, Xueji Zhang
Summary: An electrochemical sensing platform based on ATRP signal amplification strategy has been developed for ultrasensitive determination of breast and prostate cancer marker miRNA-141, with a linear range from 10 pM to 10 aM and a detection limit of 3.23 aM, providing a new and promising path for ultrasensitive analysis of tumor-related miRNAs.
Article
Chemistry, Analytical
Xianghui Li, Yilei Lu, Yiming Chen, Zhipeng Pan, Ni Lin, Zhenyu Lin, Huixing Chen
Summary: A signal-on low background electrochemiluminescence (ECL) biosensor for microRNA-141 (miRNA-141) detection was developed based on the electrostatic attraction difference between negatively charged report probes and positively charged electrode. The biosensor utilized a modified indium tin oxide (ITO) electrode with a positively charged surface to achieve low background signal by electrostatic repulsion with dichlorotris (1,10-phenanthroline) ruthenium (II) hydrate (Ru(phen)(3)(2+)). The target triggered a cascade reaction to generate negatively charged long dsDNAs, which efficiently loaded Ru(phen)(3)(2+) (Ru-dsDNA) and resulted in a strong ECL signal.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Zhiwei Sun, Juan Li, Yao Tong, Li Zhao, Xiaoyu Zhou, Hui Li, Chuanxin Wang, Lutao Du, Yanyan Jiang
Summary: The detection of miRNA shows great promise in disease diagnosis. In this study, a ratiometric fluorescent biosensor based on MWCNTs@Au NCs and DSN-assisted signal amplification was developed for miRNA detection. The biosensor exhibited a wide detection concentration range and a low limit of detection for miR-92a-3p extracted from exosomes.
Article
Chemistry, Analytical
Xin Guo, Tian Tian, Xinchun Deng, Yang Song, Xiaoming Zhou, Erqun Song
Summary: Developing rapid and accurate methods for detecting miRNA in complex samples is crucial for early disease diagnosis. Researchers have developed a magnetic relaxation switching (MRS)-based strategy for direct detection of miRNA in complex samples using the CRISPR/Cas13a signal amplification system. This method achieves accurate and rapid detection of miRNA without the need for sample extraction, and has a high detection sensitivity.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Keying Zhang, Na Zhang, Yilin Wu, Wen Zhang, Fajun Li, Yizhong Shen
Summary: The study introduces a new strategy of integrating confined emitters effect and target cycling amplification into an upconversion nanoamplicon, achieving high LRET efficiency for precise detection of cancer cell-derived miRNA levels.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Rui Chen, Chen Zhan, Chenxi Huang, Qifu He, Junwang Bao, Xiuwen Zhang, Zhixiong Pi, Yiping Chen
Summary: A novel microparticle counting immunosensor utilizing polystyrene microspheres and tyramine signal amplification was developed for sensitive detection of Aflatoxin B1 (AFB1) in contaminated food, greatly improving the sensitivity for trace detection of this toxin.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Qing Liu, Yuanyu Huang, Zhengping Li, Lele Li, Yuliang Zhao, Mengyuan Li
Summary: This study presents a method that combines enzymatically triggered catalytic hairpin assembly with lipid nanoparticle delivery strategy for tumor-specific activation of signal amplification and sensitive miRNA imaging. By introducing lipid nanoparticles, the system enables amplified miRNA imaging in vivo with improved tumor-to-background contrast.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)