期刊
PROCESSES
卷 5, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/pr5020032
关键词
cross-feeding; cyanobacteria; elementary flux mode analysis; irradiance; resource allocation; RuBisCO; stress acclimation
资金
- Office of the Provost at Montana State University through the Molecular Biosciences Program
- NSF [DMS-1361240]
- Linus Pauling Distinguished Postdoctoral Fellowship
- DOE by Battelle Memorial Institute [DE-AC05-76RLO 1830]
- BER [49356]
Metabolic acclimation to photosynthesis-associated stresses was examined in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 using integrated computational and photobioreactor analyses. A genome-enabled metabolic model, complete with measured biomass composition, was analyzed using ecological resource allocation theory to predict and interpret metabolic acclimation to irradiance, O-2, and nutrient stresses. Reduced growth efficiency, shifts in photosystem utilization, changes in photorespiration strategies, and differing byproduct secretion patterns were predicted to occur along culturing stress gradients. These predictions were compared with photobioreactor physiological data and previously published transcriptomic data and found to be highly consistent with observations, providing a systems-based rationale for the culture phenotypes. The analysis also indicated that cyanobacterial stress acclimation strategies created niches for heterotrophic organisms and that heterotrophic activity could enhance cyanobacterial stress tolerance by removing inhibitory metabolic byproducts. This study provides mechanistic insight into stress acclimation strategies in photoautotrophs and establishes a framework for predicting, designing, and engineering both axenic and photoautotrophic-heterotrophic systems as a function of controllable parameters.
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