Article
Chemistry, Multidisciplinary
Seunghun S. Lee, Nicole Kleger, Gisela A. Kuhn, Helen Greutert, Xiaoyu Du, Thijs Smit, Andre R. Studart, Stephen J. Ferguson
Summary: Digital Light Processing is used to fabricate a hive-structured assemblable bespoke scaffold (HIVE) that can be manually assembled and customized for specific defect. It can be filled with different biological factors in different areas to provide site-specific treatments. Through in vitro and in vivo experiments, it has been shown to induce osteogenesis and angiogenesis in a controlled manner.
ADVANCED MATERIALS
(2023)
Article
Engineering, Environmental
Shengnan Cheng, Yuxuan Li, Chenggong Yu, Zongwu Deng, Jie Huang, Zhijun Zhang
Summary: In this study, a biomimetic 3D co-culture metastatic model was used to accurately simulate the behavior of native breast cancer cells and investigate the interactive effects of cancer, vascular, and bone cells. The model allowed for a better understanding of drug resistance in breast cancer cells.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
P-C Chang, Z-J Lin, H-T Luo, C-C Tu, W-C Tai, C-H Chang, Y-C Chang
Summary: Recent research has focused on developing scaffolds with intricate configurations and manipulating extracellular matrix stiffness towards osteogenesis for maxillofacial defect regeneration. The study proposed infusing a degradable RGD-functionalized alginate matrix (RAM) with osteoid-like stiffness into a rigid 3D-printed hydroxyapatite scaffold. In vitro and in vivo results showed that RAM with osteoid-like stiffness, particularly when oxidized, significantly enhanced bone marrow stem cell viability, migration, and osteogenic differentiation, ultimately promoting robust bone formation in maxillofacial regeneration.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Wanheng Zhang, Yan Chen, Mengyuan Li, Shucheng Cao, Nana Wang, Yingjian Zhang, Yongtao Wang
Summary: 2D cell culture plays a crucial role in cancer research and drug discovery, but it inadequately models the biology of tumors in vivo. 3D tumor culture systems provide a better mimicry of tumor characteristics and pose significant challenges. In this study, polydopamine-modified decellularized lung scaffolds are designed to study tumor progression, drug screening, and mimic the tumor microenvironment. The modified scaffolds with enhanced hydrophilicity and cell compatibility promote cell growth and proliferation. The observed higher survival rates in the modified scaffolds after treatment with anticancer drugs suggest their potential application in drug resistance and immunotherapy screening.
Article
Pharmacology & Pharmacy
Yang Zhou, Gillian Pereira, Yuanzhang Tang, Matthew James, Miqin Zhang
Summary: Natural polymer-based porous scaffolds with tunable pore size have been developed as three-dimensional tumor models for high-throughput screening of cancer therapeutics. The CHA scaffold-based platform showed potential for mimicking the in vivo malignancy and revealed the influence of pore size on cell growth kinetics, tumor spheroid morphology, gene expression and drug response. The CHA scaffold-based HTS platform demonstrated comparable performance with commercial tissue culture plates and may provide an improved alternative for future cancer study and drug discovery.
Article
Anatomy & Morphology
Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yassin Panahi
Summary: Tissue engineering research has focused on the characteristics and biocompatibility of decellularized sheep tracheal scaffolds, as well as the differentiation of Adipose-derived stem cells (AD-MSCs) into tracheal cells. The prepared scaffolds show potential for engineering applications and provide a suitable environment for stem cell differentiation. This research serves as a foundation for future studies on tracheal decellularization scaffolds and paves the way for their use in organ regeneration and clinical medicine.
Article
Biotechnology & Applied Microbiology
Mariye Ahmadian, Saadi Hosseini, Atefeh Alipour, Mehdi Jahanfar, Naser Farrokhi, Shahin Homaeigohar, Hosein Shahsavarani
Summary: Tumor microenvironment plays a crucial role in drug response, and a 3D scaffold made from decellularized plant tissues is an ideal platform to mimic the microenvironment for testing novel anticancer drugs on liver cancer cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Shamapto Guha Anthon, Karolina Papera Valente
Summary: Traditional 2D cell culture models fail to accurately replicate the cellular environment in vivo, leading to discrepancies between preclinical and in vivo testing. Recent advancements in tissue engineering have enabled the development of 3D cell culture systems that can accurately mimic the cellular and tissue microenvironment. However, the lack of perfusion systems limits their application as drug screening platforms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Luis P. Ferreira, Vitor M. Gaspar, Luis Mendes, Iola F. Duarte, Joao F. Mano
Summary: The study explores a method for generating organotypic microtumors enriched with dECM, enabling self-assembly with tunable morphological features, with potential applications in drug screening.
Review
Oncology
Kavitha Unnikrishnan, Lynda Velutheril Thomas, Ram Mohan Ram Kumar
Summary: The lack of traditional cancer treatments has led to a demand for new clinical techniques. Utilizing 3D culture systems allows for the recreation of in vivo tumor microenvironments, leading to better modeling of human tumors and improved therapy results. The use of 3D scaffold models in cancer tissue engineering has the potential to enhance preclinical studies and facilitate clinical translation.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Nicola Contessi Negrini, Claudio Ricci, Federica Bongiorni, Luisa Trombi, Delfo D'Alessandro, Serena Danti, Silvia Fare
Summary: Development of new therapeutics to treat osteosarcoma is important, and 3D in vitro models are gaining momentum as reproducible and biomimetic tools to study tumor progression and assess new treatments. A 3D printed polyurethane scaffold enriched by in vitro pre-generated bone extracellular matrix was designed to replicate an osteosarcoma model, using osteo-differentiated human mesenchymal stromal cells. This model provides a suitable microenvironment for osteosarcoma cells and can be useful in tumor biology studies and treatment development.
Article
Pharmacology & Pharmacy
Samantha Sabetta, Davide Vecchiotti, Letizia Clementi, Mauro Di Vito Nolfi, Francesca Zazzeroni, Adriano Angelucci
Summary: Three-dimensional cell culture methods, particularly the 3D multicellular tumor spheroids model, enhance the predictive relevance of in vitro tumor models by better resembling tumor complexity and drug resistance. Recent advancements in 3D printing techniques and suitable biomaterials have provided new opportunities for developing reproducible and high-throughput 3D tissue cultures. In this study, the comparison of DU145 and U87 cancer cells cultured in the 3D spheroids model and the 3D bioprinting model showed that bioprinted cells demonstrated similar drug resistance to spheroids, suggesting a potential mechanism of drug resistance. The 3D bioprinted model utilizing alginate/gelatin hydrogel was proven to be a feasible, reproducible, and scalable model for drug screening when spheroid growth is required.
Article
Polymer Science
Adel Alshammari, Fahad Alabdah, Weiguang Wang, Glen Cooper
Summary: Large bone defects can be challenging clinically, and using polymer-based bone tissue engineered scaffolds shows promise as a solution. However, the clinical translation of such scaffolds is limited, and exploring the design space using virtual tools like mechanobiological modeling can improve this. This paper introduces a virtual design environment for scaffold development and demonstrates the potential of pore architecture in influencing bone tissue formation. The study shows that modifying pore architecture can significantly increase bone formation, suggesting that shape is as important as other design parameters for scaffold implants.
Review
Engineering, Biomedical
Xiurui Zhang, Yuwei Liu, Karen L. Clark, Anne-Marie Padget, Peter G. Alexander, Jianwu Dai, Weimin Zhu, Hang Lin
Summary: Mesenchymal stem cell-derived extracellular matrix (mECM) is gaining attention in tissue engineering due to its bioactivity, biocompatibility, and potential for autologous tissue engineering. However, current mECM methods have limitations such as low mechanical properties and lack of internal architecture. This review summarizes the development of mECM, recent methods to improve its properties, and proposes future studies for its application in regenerating tissues in humans.
BIOMEDICAL MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Wonbin Park, Ge Gao, Dong-Woo Cho
Summary: This review discusses the advances in musculoskeletal tissue engineering using 3D bioprinting technology and decellularized extracellular matrix bioink. It focuses on in vivo regeneration and in vitro modeling of musculoskeletal tissue, while also addressing current breakthroughs, limitations, and future prospects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Girdhari Rijal, Jing Wang, Ilhan Yu, David R. Gang, Roland K. Chen, Weimin Li
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Review
Biochemical Research Methods
Girdhari Rijal, Weimin Li
JOURNAL OF BIOLOGICAL ENGINEERING
(2018)
Editorial Material
Cell Biology
Song-Tao Liu, Gordon K. Chan, Weimin Li
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2019)
Article
Acoustics
Lokesh Basavarajappa, Girdhari Rijal, Kenneth Hoyt
Summary: The study investigated various multi-focused ultrasound (FUS) strategies for enhancing microvascular permeability in cancer therapy. Results showed that multi-FUS with temporal sequential excitation (multi-FUS-TSE) group exhibited significantly higher intratumoral dye accumulation and fluorescence measurements compared to other therapy groups. This demonstrates that the spatial and temporal aspects of FUS therapy play a crucial role in increasing microvascular permeability.
IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL
(2021)
Article
Biotechnology & Applied Microbiology
Girdhari Rijal
BIOENGINEERING-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Girdhari Rijal
Summary: This review paper discusses the migration, activation, differentiation, secretion, and signaling systems associated with fibroblasts and cancer-associated fibroblasts (CAFs) after implantation of a breast tumoroid, i.e., a lab-generated tumor tissue into an animal.
BIOENGINEERING-BASEL
(2021)
Proceedings Paper
Engineering, Electrical & Electronic
Mawia Khairalseed, Girdhari Rijal, Kenneth Hoyt
PROCEEDINGS OF THE 2020 IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM (IUS)
(2020)
Proceedings Paper
Engineering, Electrical & Electronic
Girdhari Rijal, Kenneth Hoyt
PROCEEDINGS OF THE 2020 IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM (IUS)
(2020)
Proceedings Paper
Engineering, Electrical & Electronic
Lokesh Basavarajappa, Shreya Reddy, Haowei Tai, Jane Song, Girdhari Rijal, Kevin J. Parker, Kenneth Hoyt
PROCEEDINGS OF THE 2020 IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM (IUS)
(2020)
Article
Oncology
Yoshinari Shinsato, Andrew D. Doyle, Weimin Li, Kenneth M. Yamada
Article
Oncology
Kelsey F. Ruud, William C. Hiscox, Ilhan Yu, Roland K. Chen, Weimin Li
BREAST CANCER RESEARCH
(2020)
Proceedings Paper
Engineering, Electrical & Electronic
Ipek Oezdemir, Kulsoom Javed, Girdhari Rijal, Kenneth Hoyt
2019 IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM (IUS)
(2019)
Proceedings Paper
Engineering, Electrical & Electronic
Lokesh Basavarajappa, Girdhari Rijal, Kenneth Hoyt
2019 IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM (IUS)
(2019)