Article
Immunology
Su Jeong Lee, Bo Ruem Yoon, Hee Young Kim, Su-Jin Yoo, Seong Wook Kang, Won-Woo Lee
Summary: The study demonstrates that activated platelets induce CD16 expression on classical CD14(+)CD16(-) monocytes through soluble factors, contributing to antibody-dependent cellular phagocytosis (ADCP). CD14(+)CD16(-) monocytes treated with activated platelets preferentially differentiate into M2 macrophages, and elevated levels of the activated platelet marker sCD62P are found in plasma of rheumatoid arthritis patients.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Raghunathan Jagannathan, Thodur Madapusi Balaji, Saranya Varadarajan, B. Bhuvaneswari, C. J. Venkatakrishnan, S. Lakshmi Priya, Swaminathan Rajendran, Mohammed E. Sayed, Maryam H. Mugri, Shankargouda Patil
Summary: Periodontitis has been suggested as a significant risk factor for adverse pregnancy outcomes, with elevated levels of periodontal pathogenic bacteria and proinflammatory cytokines believed to contribute to preterm delivery through placental infection and premature rupture of the amniotic membrane. The role of CD14+CD16+ monocytes in this process has not been widely researched, but potential mechanistic links between periodontitis and adverse pregnancy outcomes are being explored.
MEDICAL HYPOTHESES
(2021)
Article
Medicine, General & Internal
Nik Syazana Izyan Saffery, Krishnamurithy Genasan, Chee Ken Chan, Khairul Anwar Ayob, Seow Hui Teo, Mohamed Zubair Mohamed Al-Fayyadh, Iekhsan Othman, Syafiq Asnawi Zainal Abidin, Murali Malliga Raman, Hanumantha Rao Balaji Raghavendran, Tunku Kamarul
Summary: This study found that osteoarthritis synovium has a specific protein profile that can cause migration, activation, and functional commitment of monocytes. These findings may have important implications for the treatment of osteoarthritis.
FRONTIERS IN MEDICINE
(2022)
Article
Immunology
Qinglei Yin, Dalong Song, Juan Chen, Guang Ning, Weiqing Wang, Shu Wang
Summary: CD14++CD16+ monocytes play a critical role in the pathogenesis of Graves' disease and promote the generation of potentially pathogenic Th responses in GD.
CLINICAL IMMUNOLOGY
(2022)
Article
Medicine, General & Internal
Abelardo Caballero, Teresa Vazquez-Sanchez, Pedro Ruiz-Esteban, Myriam Leon, Juana Alonso-Titos, Veronica Lopez, Eugenia Sola, Elena Gutierrez, Mercedes Cabello, Cristina Casas-Gonzalez, Rafael Pozo-Alvarez, Juan Delgado-Burgos, Domingo Hernandez
Summary: The study revealed that CD14++CD16+ monocytes in low-immunological-risk kidney transplant recipients were significantly lower in cases of subclinical borderline lesions, potentially serving as an early indicator for rejection.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Immunology
Reiko Takimoto, Tetsuo Suzawa, Atsushi Yamada, Kiyohito Sasa, Yoichi Miyamoto, Kentaro Yoshimura, Yuji Sasama, Motohiro Tanaka, Mitsuhiro Kinoshita, Kaori Ikezaki, Makoto Ichikawa, Matsuo Yamamoto, Tatsuo Shirota, Ryutaro Kamijo
Summary: Zoledronate induces acute-phase reactions in the immune system, requiring the involvement of gamma delta T cells. CD14(+) cells are responsible for the production of IL-1 beta, IL-6, and IFN-gamma in the blood exposed to zoledronate, indicating their essential role in APRs following N-BP administration.
Article
Medicine, General & Internal
Haoyang Sun, Raquel A. Binder, Borame Dickens, Paola Florez de Sessions, Maia A. Rabaa, Eliza Xin Pei Ho, Alex R. Cook, Fausto Bustos Carrillo, Jairo Carey Monterrey, Guillermina Kuan, Angel Balmaseda, Eng Eong Ooi, Eva Harris, October M. Sessions
Summary: Through analyzing viral genomes, it was found that community transmission, often involving long geographical distances, played a more significant role in the spread of the Zika epidemic in Nicaragua compared to household transmission. The study also identified potential super-spreading events and transmission events across long distances within the estimated transmission tree.
Article
Biochemistry & Molecular Biology
Kirsten Glaser, David Kern, Christian P. Speer, Nicolas Schlegel, Michael Schwab, Ulrich H. Thome, Christoph Haertel, Clyde J. Wright
Summary: Developmentally regulated features of innate immunity in preterm and term infants may increase the risk of infection and inflammation-related morbidity. This study investigated the cytokine expression and monocyte subsets in cord blood monocytes of preterm and term infants compared to adult controls. The results showed robust pro-inflammatory but attenuated anti-inflammatory responses, as well as imbalanced cytokine ratios, in cord blood monocytes of preterm and term infants. Intermediate monocytes, known for their pro-inflammatory features, may contribute to this inflammatory state.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Chang-Han Chuang, Chi-Chung Kuo, Yueh-Feng Chiang, Pei-Yuan Lee, Fu-Hui Wang, Chia-Ying Hsieh, Ching- Shen, Yu-Hsuan Chung, Kuan-Der Lee, Shih-Fang Wu, Hong-Lin Su, Chih-Lung Lin
Summary: This study examines the effect of peripheral blood-derived mononuclear cells (PBMNCs) on patients with knee osteoarthritis (KOA). The study found that intra-articular injection of autologous PBMNCs significantly attenuated knee pain and motor dysfunction in patients, and the therapeutic effect could be stably maintained for up to 24 months.
CELL TRANSPLANTATION
(2023)
Article
Multidisciplinary Sciences
He Zhang, Shi-lei Wang, Tao Sun, Jia Liu, Ping Li, Jing-ci Yang, Fang Gao
Summary: Collateral formation in patients with acute myocardial infarction (AMI) is associated with CD14++CD16+ monocytes and vascular endothelial growth factors (VEGFs). In a study of 49 AMI patients, it was found that patients with early collateral formation had significantly higher levels of CD14++CD16+ monocytes and serum VEGFs compared to patients with late collateral formation. Additionally, serum VEGF levels were positively correlated with CD14++CD16+ monocytes. These results suggest that regulating CD14++CD16+ monocytes and VEGFs is crucial for collateral formation and AMI healing.
Article
Medicine, General & Internal
Vincent M. Tutino, Haley R. Zebraski, Hamidreza Rajabzadeh-Oghaz, Muhammad Waqas, James N. Jarvis, Konrad Bach, Maxim Mokin, Kenneth V. Snyder, Adnan H. Siddiqui, Kerry E. Poppenberg
Summary: PBMCs play a crucial role in the inflammation associated with intracranial aneurysm (IA) pathophysiology. By analyzing PBMC RNA from patients with and without IAs, researchers identified a signature of 54 genes associated with IA, which could potentially serve as blood-based biomarkers for the disease. Further bioinformatics analyses highlighted key processes and pathways involved in IA pathophysiology, and correlation analysis revealed certain genes associated with rupture risk, indicating their potential as predictive biomarkers.
Article
Multidisciplinary Sciences
Ryosuke Shirakawa, Takayuki Nakajima, Aya Yoshimura, Yukako Kawahara, Chieko Orito, Miwako Yamane, Haruka Handa, Shingo Takada, Takaaki Furihata, Arata Fukushima, Naoki Ishimori, Masao Nakagawa, Isao Yokota, Hisataka Sabe, Satoshi Hashino, Shintaro Kinugawa, Takashi Yokota
Summary: Systemic inflammation is associated with the link between obesity and nonalcoholic fatty liver disease (NAFLD). In this study, researchers investigated changes in leukocytes' mitochondria in obese individuals and their correlation with NAFLD. The findings suggest that obese individuals have increased mitochondrial respiratory capacity in peripheral blood mononuclear cells (PBMCs), and this enhanced oxidative metabolism is associated with hepatic steatosis in obese young adults.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Zhen Wang, Lijian Xie, Guohui Ding, Sirui Song, Liqin Chen, Guang Li, Min Xia, Dingding Han, Yue Zheng, Jia Liu, Tingting Xiao, Hong Zhang, Yujuan Huang, Yixue Li, Min Huang
Summary: Immune cell changes are associated with Kawasaki disease pathogenesis, with monocyte inflammatory genes over-expressed and oligoclonal expansions observed in TCR and BCR clonotype sequences after IVIG therapy. The study provides insights into the pathogenesis and therapy of KD by profiling PBMC from patients before and after treatment, identifying differential gene expression and changes in B cell and T cell percentages, as well as oligoclonal expansions.
NATURE COMMUNICATIONS
(2021)
Article
Cardiac & Cardiovascular Systems
Karin Anne Lydia Mueller, Carolin Langnau, Manina Guenter, Simone Poeschel, Sarah Gekeler, Alvaro Petersen-Uribe, Klaus-Peter Kreisselmeier, Karin Klingel, Hans Boesmueller, Bo Li, Philippa Jaeger, Tatsiana Castor, Dominik Rath, Meinrad Paul Gawaz, Stella E. Autenrieth
Summary: Decreased numbers of CD14(dim)CD16(+) monocytes are associated with an increased risk of rapidly progressive respiratory failure in patients with both coronary artery disease (CAD) and SARS-CoV-2 infection. Intensified risk assessments involving monocyte subtypes and phenotypes may help identify patients at higher risk for respiratory failure.
CARDIOVASCULAR RESEARCH
(2021)
Article
Medicine, General & Internal
Julia Vallve-Juanico, Ashley F. George, Sushmita Sen, Reuben Thomas, Min-Gyoung Shin, Divyashree Kushnoor, Joshua J. Vasquez, Kim Chi Vo, Juan C. Irwin, Nadia R. Roan, Alexis J. Combes, Linda C. Giudice
Summary: This study utilized mass cytometry and high dimensional analyses to study immune cell phenotypes, abundance, activation states, and functions in women with and without endometriosis in different cycle phases and disease stages. The findings revealed a greater inflammatory phenotype of endometrial macrophages in endometriosis and systemic aberrant functionality of the myeloid system.
Article
Multidisciplinary Sciences
Leah C. Katzelnick, Lionel Gresh, M. Elizabeth Halloran, Juan Carlos Mercado, Guillermina Kuan, Aubree Gordon, Angel Balmaseda, Eva Harris
Article
Microbiology
Dustin R. Glasner, Kalani Ratnasiri, Henry Puerta-Guardo, Diego A. Espinosa, P. Robert Beatty, Eva Harris
Article
Immunology
Diego A. Espinosa, P. Robert Beatty, Gabrielle L. Reiner, Kelsey E. Sivick, Laura Hix Glickman, Thomas W. Dubensky, Eva Harris
JOURNAL OF IMMUNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Diego A. Espinosa, P. Robert Beatty, Henry Puerta-Guardo, M. Nurul Islam, John T. Belisle, Rushika Perera, Eva Harris
JOURNAL OF GENERAL VIROLOGY
(2019)
Article
Multidisciplinary Sciences
Daniela Andrade, Colin Warnes, Ellen Young, Leah C. Katzelnick, Angel Balmaseda, Aravinda M. de Silva, Ralph S. Baric, Eva Harris
SCIENTIFIC REPORTS
(2019)
Article
Infectious Diseases
Cynthia A. Tschampl, Eduardo A. Undurraga, Robert J. Ledogar, Josefina Coloma, Jose Legorreta-Soberanis, Sergio Paredes-Solis, Jorge Arostegui, Carlos Hernandez-Alvarez, Eva Harris, Neil Andersson, Donald S. Shepard
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Multidisciplinary Sciences
Sara Borghi, Stylianos Bournazos, Natalie K. Thulin, Chao Li, Anna Gajewski, Robert W. Sherwood, Sheng Zhang, Eva Harris, Prasanna Jagannathan, Lai-Xi Wang, Jeffrey V. Ravetch, Taia T. Wang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Laura W. Alexander, Rotem Ben-Shachar, Leah C. Katzelnick, Guillermina Kuan, Angel Balmaseda, Eva Harris, Mike Boots
Summary: Research suggests that frequent boosting of antibodies in dengue-endemic areas can protect the immune response against symptomatic dengue virus infection, explaining the fluctuations in the ratio of asymptomatic to symptomatic infections. These findings highlight the importance of strengthening dengue control efforts.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Thomas P. Burke, Patrik Engstrom, Cuong J. Tran, Ingeborg M. Langohr, Dustin R. Glasner, Diego A. Espinosa, Eva Harris, Matthew D. Welch
Summary: Mice lacking both interferon receptors provide a tractable model for investigating rickettsiosis, virulence factors, and immunity. The study shows that the actin-based motility factor Sca2 is required for dissemination from the skin to internal organs, and the outer membrane protein OmpB contributes to eschar formation, revealing potential live-attenuated vaccine candidates. Differences in interferon signaling may underlie the discrepancies in susceptibility to rickettsial pathogens between mice and humans.
Article
Virology
Nicholas T. N. Lo, Susan Z. Roodsari, Nicole L. Tin, Marcus P. Wong, Scott B. Biering, Eva Harris
Summary: Flavivirus NS1 is a viral protein that is secreted into the bloodstream during an infection. It plays a role in severe dengue pathology such as endothelial dysfunction and vascular leak. In this study, we investigated the molecular determinants that allow some flavivirus NS1 proteins to bind to specific endothelial cells and cause vascular leak in a mouse model. We found that the wing domain of NS1 is a primary determinant for endothelial dysfunction and vascular leak, and narrowed down the contributing amino acid residues to a three-residue motif within the wing domain.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Veena S. Patil, Ariel Madrigal, Benjamin J. Schmiedel, James Clarke, Patrick O'Rourke, Aruna D. de Silva, Eva Harris, Bjoern Peters, Gregory Seumois, Daniela Weiskopf, Alessandro Sette, Pandurangan Vijayanand
SCIENCE IMMUNOLOGY
(2018)