期刊
ACS SENSORS
卷 2, 期 2, 页码 210-217出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.6b00581
关键词
interdigitated electrode array; electrochemical impedance spectroscopy; interferon gamma; aptamer capture probe; ternary surface monolayer; equivalent circuit model
资金
- Roy J. Carver Trust Foundation [15-4615]
- department of Mechanical Engineering, Veterinary Medical School of Iowa State University
- College of Engineering, Veterinary Medical School of Iowa State University
A label-free electrochemical impedance spectroscopy (EIS) aptasensor for rapid detection (<35 min) of interferon-gamma (IFN-gamma) was fabricated by immobilizing a RNA aptamer capture probe (ACP), selective to IFN-gamma, on a gold interdigitated electrode array (Au IDE). The ACP was modified with a thiol group at the 5' terminal end and subsequently co immobilized with 1,6-hexanedithiol (HDT) and 6-mercapto-l-hexanolphosphate (MCH) to the gold surface through thiol gold interactions. This ACP/HDT-MCH ternary surface monolayer facilitates efficient hybridization with IFN-gamma and displays high resistance to nonspecific adsorption of nontarget proteins [i.e., fetal bovine serum (FBS) and bovine serum albumin (BSA)]. The Au IDE functionalized with ACP/HDT-MCH was able to measure IFN-gamma in actual FBS solution with a linear sensing range from 22.22 pM to 0.11 nM (1-5 ng/mL) and a detection limit of 11.56 pM. The ability to rapidly sense IFN-gamma within this sensing range makes the developed electrochemical platform conducive toward in-field disease detection of a variety of diseases including paratuberculosis (i.e., Johne's Disease). Furthermore, experimental results were numerically validated with an equivalent circuit model that elucidated the effects of the sensing process and the influence of the immobilized ternary monolayer on signal output. This is the first time that ternary surface monolayers have been used to selectively capture/detect IFN-gamma on Au IDEs.
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