Review
Genetics & Heredity
Xianmei Zhong, Jie Chen, Bo Wen, Xu Wu, Mingxing Li, Fukuan Du, Yu Chen, Shuai Deng, Yueshui Zhao, Jing Shen, Zhangang Xiao
Summary: Immunosenescence, characterized by immunodeficiency and inflammation, occurs with age. This review explores the potential role of mesenchymal stem cells (MSCs) in protecting against T cell aging, providing new insights for intervening in immune system aging.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Cell & Tissue Engineering
Asmaa A. Zidan, Griffith B. Perkins, Mohammed Al-Hawwas, Ahmed Elhossiny, Jianyu Yang, Larisa Bobrovskaya, Ghada M. Mourad, Xin-Fu Zhou, Plinio R. Hurtado
Summary: USCs have the ability to inhibit proliferation and induce apoptosis of activated T lymphocytes, while also activating, proliferating, and inducing cytokine and antibody production of B lymphocytes.
Article
Cell Biology
Qiuli Liu, Xiaoyong Chen, Chang Liu, Lijie Pan, Xinmei Kang, Yanli Li, Cong Du, Shuai Dong, Andy Peng Xiang, Yan Xu, Qi Zhang
Summary: CHI3L1 is a novel immunosuppressive factor secreted by MSCs, which plays a crucial role in inhibiting T cell activation and inflammatory responses in immune-mediated liver injury. Downregulating CHI3L1 in hUC-MSCs mitigates their therapeutic effects on liver injury by affecting T cell function. Additionally, CHI3L1 exerts its immunosuppressive effects by upregulating PPAR δ to inhibit the JAK/STAT signaling pathway in T cells.
CELL DEATH & DISEASE
(2021)
Review
Immunology
Yongsheng Luo, Jingjing Guo, Pingbao Zhang, Yin Celeste Cheuk, Yamei Jiang, Jiyan Wang, Shihao Xu, Ruiming Rong
Summary: The increase in Th17-mediated pro-inflammatory response and decrease in Treg-mediated anti-inflammatory effect aggravate renal tubular epithelial cell injury. MSC can control the imbalance between Th17 and Treg by inhibiting mTOR signaling, promoting Treg generation while restraining Th17 differentiation. This suggests that MSC-mediated Th17-to-Treg polarization could serve as a new immunotherapy approach for kidney injury.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yue Ke, Yu Ye, Jintao Wu, Yanxia Ma, Yuxin Fang, Fei Jiang, Jinhua Yu
Summary: A phosphoserine-loaded chitosan membrane was synthesized to improve the osteogenic effect of chitosan membranes. The incorporation of phosphoserine into chitosan membranes was confirmed and the phosphoserine-chitosan membranes significantly stimulated bone regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jiali Fang, Zheng Chen, Xingqiang Lai, Wei Yin, Yuhe Guo, Weiting Zhang, Junjie Ma, Guanghui Li, Lei Zhang
Summary: Pancreatic islet transplantation is hindered by hypoxia-induced cell death. This study demonstrates that HIF-1α-EVs derived from MSCs can enhance the viability of pancreatic beta cells under hypoxic conditions by promoting protective autophagy and inhibiting apoptosis and senescence.
BIOORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Hala M. ElBadre, Sahar E. M. El-Deek, Haidi Karam-Allah Ramadan, Mohamed M. Elbadr, Dina Sabry, Noran M. Ahmed, Amr M. Ahmed, Reham El-Mahdy
Summary: In this study, the effects of umbilical cord mesenchymal stem cells (UC-MSCs) derived exosomes on HepG2 cell line and the underlying mechanism of controlling hepatocellular carcinoma (HCC) proliferation were investigated. The results showed that treatment with exosomes significantly reduced the survival of HepG2 cells and altered the expression levels of SIRT-1, SDF-1, CXCR-4, TNF-α, and caspase-3. Furthermore, the anti-proliferative, apoptotic, and anti-angiogenic effects of exosomes on HepG2 cells were time-dependent. These findings suggest that exosomes could be a potential novel therapy for HCC, but further large-scale studies are needed to confirm this conclusion.
Article
Cell Biology
Agnese Fiori, Stefanie Uhlig, Harald Kluter, Karen Bieback
Summary: Mesenchymal stromal cells (MSC) have immunomodulatory effects on various immune cells. In this study, human adipose tissue-derived MSC (ASC) were found to inhibit CD4 + T cell proliferation, induce Treg cells, and modulate cytokine secretion, suggesting a potential role in immune regulation. These findings highlight the complex interplay between ASC and CD4 + T cells.
Review
Cell & Tissue Engineering
Zhiping Wei, Jintao Yuan, Gaoying Wang, Dickson Kofi Wiredu Ocansey, Zhiwei Xu, Fei Mao
Summary: Research has shown that MSCs have potential therapeutic effects in autoimmune diseases. In addition to treating fractures and tissue repair, MSCs can help restore immune homeostasis by regulating T cell subsets.
STEM CELLS INTERNATIONAL
(2021)
Article
Cell Biology
Denise Zujur, Ziadoon Al-Akashi, Anna Nakamura, Chengzhu Zhao, Kazuma Takahashi, Shizuka Aritomi, William Theoputra, Daisuke Kamiya, Koichi Nakayama, Makoto Ikeya
Summary: We developed a stepwise differentiation method to generate matrix-rich cartilage spheroids from induced pluripotent stem cell-derived mesenchymal stem/stromal cells (iMSCs) via the induction of neural crest cells under xeno-free conditions. By using a thienoindazole derivative, TD-198946, we achieved enhanced chondrogenesis in iMSCs. Our findings provide a novel cell source for stem cell-based cartilage repair and offer the potential for biofabrication of larger cartilage tissues using chondrogenic spheroids and technologies such as Kenzan Bioprinting.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Retraction
Cell & Tissue Engineering
Zhanjun Ma, Xue Cui, Li Lu, Guohu Chen, Yang Yang, Yan Hu, Yubao Lu, Zhangqi Cao, Yan Wang, Xuexi Wang
Summary: The article has been retracted. More details can be found in the Retraction Notice at https://doi.org/10.1186/s13287-019-1149-5.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Dong Hoon Kwak, Ji Hye Park, Eul Sig Choi, Seong Hyun Park, Seo-Yeon Lee, Seoul Lee
Summary: This study analyzed the role of gangliosides in the osteogenesis of Lmna dysfunctional MSCs. The results showed that ganglioside GD1a played an important role in enhancing osteogenic differentiation in Lmna-mutated MSCs.
Review
Pharmacology & Pharmacy
Andrea Cassinotti, Francesco Passamonti, Sergio Segato
Summary: Cell-based therapies have been explored in immune-mediated inflammatory diseases, including inflammatory bowel disease (IBD). The most studied treatment in IBD involves stem cells, with recent studies also focusing on regulatory cells such as T-reg and Tr1 cells. Preclinical research is also being conducted on induced pluripotent stem cells in animal models related to colitis.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Paula Laranjeira, Francisco dos Santos, Maria Joao Salvador, Irina N. Simoes, Carla M. P. Cardoso, Barbara M. Silva, Helena Henriques-Antunes, Luisa Corte-Real, Sofia Couceiro, Filipa Monteiro, Carolina Santos, Tania Santiago, Jose A. P. da Silva, Artur Paiva
Summary: Systemic sclerosis (SSc) is an immune-mediated disease with poor prognosis and limited therapeutic options. Mesenchymal-stem/stromal-cell (MSC)-based therapies have potential benefits for SSc patients due to their immunomodulatory, anti-fibrotic, and pro-angiogenic properties. This study found that MSCs downregulated the activation of T cell subsets and affected their polarization in SSc patients, including subsets implicated in SSc's pathogenesis. This provides further support for the potential of MSC-based therapies to regulate T cells in SSc.
Article
Medicine, Research & Experimental
Laurens Raes, Melissa Pille, Aranit Harizaj, Glenn Goetgeluk, Jelter Van Hoeck, Stephan Stremersch, Juan C. Fraire, Toon Brans, Olivier Gerrit de Jong, Roel Maas-Bakker, Enrico Mastrobattista, Pieter Vader, Stefaan C. De Smedt, Bart Vandekerckhove, Koen Raemdonck, Kevin Braeckmans
Summary: The study successfully demonstrated Cas9 RNP transfection in various cell types using VNB photoporation, including refractive cells and achieving high gene knock-out levels. Gene knock-out according to user-defined spatial patterns was shown in stem cells and T cells. VNB photoporation is a scalable and versatile intracellular delivery method with great promise for CRISPR-Cas9-mediated ex vivo engineering of cell therapy products.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Biochemistry & Molecular Biology
Young-In Kim-Hoehamer, Janice M. Riberdy, Fei Zheng, Jeoungeun J. Park, Na Shang, Jean-Yves Metais, Timothy Lockey, Catherine Willis, Salem Akel, Jennifer Moore, Michael M. Meagher, M. Paulina Velasquez, Brandon M. Triplett, Aimee C. Talleur, Stephen Gottschalk, Sheng Zhou
Summary: We have successfully developed a current good manufacturing practice-compliant process to manufacture allogeneic CD19-CAR T cells, which have shown potent antitumor activity in vitro and in vivo.
Article
Multidisciplinary Sciences
Nikhil Hebbar, Rebecca Epperly, Abishek Vaidya, Unmesha Thanekar, Sarah E. Moore, Masayuki Umeda, Jing Ma, Sagar L. Patil, Deanna Langfitt, Sujuan Huang, Cheng Cheng, Jeffery M. Klco, Stephen Gottschalk, M. Paulina Velasquez
Summary: The study demonstrates that targeting cell surface GRP78-positive AML with CAR T cells is feasible and shows significant anti-AML activity in preclinical models. The findings suggest that GRP78 could be a suitable target for CAR-T therapy in patients with acute myeloid leukemia.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Hong Mu-Mosley, Lauren Ostermann, Muharrem Muftuoglu, Abishek Vaidya, Challice L. Bonifant, Mireya Paulina Velasquez, Stephen Gottschalk, Michael Andreeff
Summary: In this study, transgenic expression of IL15 was found to significantly improve the expansion, persistence, and anti-AML activity of CD123-ENG T-cells, promoting a naive/TSCM-like phenotype. However, targeting a single tumor antigen may lead to immune escape, emphasizing the need for approaches targeting multiple antigens. Furthermore, the evaluation of autologous T cells in AML PDX models was shown to be feasible.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Jaquelyn T. Zoine, Sarah E. Moore, M. Paulina Velasquez
Summary: This paper discusses preclinical modeling of leukemia ACT and emphasizes the importance of syngeneic mouse models. Syngeneic models can better evaluate the impact of immunosuppressive leukemia microenvironment on treatment outcomes, contributing to the improvement of leukemia therapy strategies.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Hematology
Aimee C. Talleur, Amr Qudeimat, Jean-Yves Metais, Deanna Langfitt, Ewelina Mamcarz, Jeremy Chase Crawford, Sujuan Huang, Cheng Cheng, Caitlin Hurley, Renee Madden, Akshay Sharma, Ali Suliman, Ashok Srinivasan, M. Paulina Velasquez, Esther A. Obeng, Catherine Willis, Salem Akel, Seth E. Karol, Hiroto Inaba, Allison Bragg, Wenting Zheng, Sheng M. Zhou, Sarah Schell, Macal Tuggle-Brown, David Cullins, Sagar L. Patil, Ying Li, Paul G. Thomas, Caitlin Zebley, Benjamin Youngblood, Ching-Hon Pui, Timothy Lockey, Terrence L. Geiger, Michael M. Meagher, Brandon M. Triplett, Stephen Gottschalk
Summary: CD19-specific CAR T cells have potent antileukemia activity in pediatric and adult B-ALL patients, but not all patients achieve a complete response. This clinical study evaluated a new CD19-CAR T cell product in pediatric patients and showed that the treatment was safe and effective, with disease burden before infusion affecting the treatment outcome.
Article
Hematology
Abdullah Freiwan, Jaquelyn T. Zoine, Jeremy Chase Crawford, Abishek Vaidya, Stefan A. Schattgen, Jacquelyn A. Myers, Sagar L. Patil, Mahsa Khanlari, Hiroto Inaba, Jeffery M. Klco, Charles G. Mullighan, Giedre Krenciute, Peter J. Chockley, Swati Naik, Deanna M. Lang, Maksim Mamonkin, Esther A. Obeng, Paul G. Thomas, Stephen Gottschalk, Paulina Velasquez
Summary: CD7(-) T cells can serve as a promising subset for CAR-T cell therapy of T-ALL and other hematological malignancies.
Article
Oncology
Taylor L. Wilson, Hyunjin Kim, Ching-Heng Chou, Deanna Langfitt, Robert C. Mettelman, Anastasia A. Minervina, E. Kaitlynn Allen, Jean-Yves Metals, Mikhail V. Pogorelyy, Janice M. Riberdy, M. Paulina Velasquez, Pratibha Kottapalli, Sanchit Trivedi, Scott R. Olsen, Timothy Lockey, Catherine Willis, Michael M. Meagher, Brandon M. Triplett, Aimee C. Talleur, Stephen Gottschalk, Jeremy Chase Crawford, Paul G. Thomas
Summary: By analyzing single-cell sequencing data, we identified unique effector precursor cells in preinfusion CAR T-cell products with higher functional potential and resistance to exhaustion.
Meeting Abstract
Hematology
Sarah E. Moore, Jaquelyn T. Zoine, Jeremy Chase Crawford, Deanna Langfitt, Juan M. Barajas, Sherif Abdelhamed, Ilaria Iacobucci, Dalia Haydar, Giedre Krenciute, Charles G. Mullighan, Jeffery M. Klco, Stephen Gottschalk, Paul G. Thomas, Mireya Paulina Velasquez
Meeting Abstract
Hematology
Lauren Scherer, Candise Tat, Tim Sauer, Haruko Tashiro, Swati Naik, Mireya Paulina Velasquez, Stephen Gottschalk, Cliona M. Rooney, Malcolm K. Brenner, Bilal Omer
Meeting Abstract
Hematology
Swati Naik, Renee M. Madden, Amanda Lipsitt, Timothy Lockey, Jennyfer Bran, Jeffrey E. Rubnitz, Jeffery Klco, Barry Shulkin, Sagar L. Patil, Sarah Schell, Jeoungeun John Park, Janice Riberdy, Na Shang, Jaquelyn T. Zoine, Jennifer Wallace, Elaine Harstead, Catherine Willis, Jean-Yves Metais, Deanna M. Langfitt, Sheng Zhou, Salem M. Akel, Michael M. Meagher, Brandon M. Triplett, Stephen Gottschalk, Mireya Paulina Velasquez
Letter
Biophysics
Thomas Pfeiffer, Ying Li, Emily Ashcraft, Seth E. Karol, Jeffrey E. Rubnitz, Rebecca Epperly, Renee Madden, Ewelina Mamcarz, Esther Obeng, Amr Qudeimat, Akshay Sharma, Ashok Srinivasan, Ali Suliman, Aimee C. Talleur, M. Paulina Velasquez, Stephen Gottschalk, Brandon M. Triplett, Swati Naik
BONE MARROW TRANSPLANTATION
(2023)
Editorial Material
Cell Biology
Verena van der Heide, Etienne Humblin, Abishek Vaidya, Alice Oliffson Kamphorst
Summary: Chronic antigen stimulation leads to T cell exhaustion, which is further exacerbated by nutrient restrictions and other suppressive factors in the tumor microenvironment. Understanding the heterogeneity and dynamics of exhausted CD8 T cells will guide the development of novel therapies to modulate T cell differentiation for more effective anti-tumor responses.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Meeting Abstract
Biotechnology & Applied Microbiology
Jaquelyn T. Zoine, Nikhil Hebbar, Sarah E. Moore, Deanna M. Langfitt, Jorge A. Ibanez, Lindsay Talbot, Giedre Krenciute, Stephen Gottschalk, M. Paulina Velasquez
Meeting Abstract
Oncology
Thomas Pfeiffer, Ying Li, Seth E. Karol, Jeffrey E. Rubnitz, Rebecca Epperly, Renee Madden, Ewelina Mamcarz, Esther A. Obeng, Amr Qudeimat, Akshay Sharma, Ashok Srinivasan, Ali Suliman, Aimee C. Talleur, Mireya Paulina Velasquez, Stephen Gottschalk, Brandon M. Triplett, Swati Naik
PEDIATRIC BLOOD & CANCER
(2022)