Article
Biochemistry & Molecular Biology
Zhiyuan Sui, Zhishuai Zhang, Yongjie Zhang, Jihu Zhang, Qingjin Li, Feng Xing
Summary: This study focused on the correlation between the methylation status of CpG islands in the promoter region of the Lin28B gene and different growth periods in Dolang sheep's hypothalamus. The results showed that the Lin28B gene promoter region contained a CpG island with 15 transcription factor binding sites and 12 CpG sites. Methylation levels increased from prepuberty to postpuberty, while Lin28B expression levels decreased, indicating a negative correlation between Lin28B expression and promoter methylation levels. Variance analysis revealed significant differences in the methylation status of CpG5, CpG7, and CpG9 between pre- and postpuberty.
DNA AND CELL BIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Dahui Wang, Yifan Wen, Zijing Zhang, Shizhen Yang, Xian Liu, Cuicui Cai, Qingming An, Shijie Lyu, Hua He, Jianliang Xie, ChuZhao Lei, Hong Chen, Baorui Ru, Eryao Wang, Yongzhen Huang
Summary: DNA methylation plays a key role in gene expression and muscle tissue development. This study examined DNA methylation and gene expression in adipose and muscle tissues simultaneously, focusing on the differentially methylated region (DMR) in SERPINA3. The results showed significant differences in DNA methylation patterns and mRNA levels between Jiaxian cattle and other breeds, suggesting an important role of DNA methylation in tissue development.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lei Zhao, Xiaohong Wu, Junnian Zheng, Dong Dong
Summary: In this study, an efficient workflow LCM-mu WGBS was introduced to profile the DNA methylome of circulating tumor cells from lung cancer patients. A unique DNA methylation signature of CTCs distinct from primary lung cancer tissues was identified, with promoter hypermethylation of epithelial genes as a hallmark of stable epithelial-mesenchymal transition. Furthermore, CTCs may exhibit a stemness-related feature during dissemination and metastasis, providing potential noninvasive CTC DNA methylation biomarkers for clinical diagnosis.
Article
Oncology
Hossein Dehdari, Fatemeh Moradian, Ali Barzegar, Mohammed Ali Ebirahimzadeh
Summary: This study found that increased transcription of CYP1A1 in breast cancer is correlated with hypermethylation of a specific CpG block and upregulation of antiapoptotic protein BCL-2. The hypermethylation of this CpG block may serve as a potential biomarker for diagnosis and progression of breast cancer.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Medicine, Research & Experimental
Donghyun D. Lee, Martin Komosa, Sumedha Sudhaman, Ricardo Leao, Cindy H. Zhang, Joana D. Apolonio, Thomas Hermanns, Peter J. Wild, Helmut Klocker, Farshad Nassiri, Gelareh Zadeh, Bill H. Diplas, Hai Yan, Steven Gallinger, Trevor J. Pugh, Vijay Ramaswamy, Michael D. Taylor, Pedro Castelo-Branco, Nuno Miguel Nunes, Uri Tabori
Summary: The research found that allele-specific DNA methylation within the TERT promoter plays a significant regulatory role in cancer, especially more common in adult cancers and enriched in tumors with TERT promoter mutations. This allele-specific DNA methylation is essential for the regulation of TERT expression in cancer.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Agriculture, Dairy & Animal Science
Ewelina Semik-Gurgul, Tomasz Zabek, Ewelina Kawecka-Grochocka, Magdalena Zalewska, Ewa Kosciuczuk, Emilia Bagnicka
Summary: Mastitis is a common disease in dairy cows, leading to economic losses. Pathogen invasion is the main cause of elevated milk somatic cell count. Staphylococcus aureus and coagulase-negative staphylococci are major pathogens responsible for mastitis.
ANNALS OF ANIMAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Andrea Lauria, Guohua Meng, Valentina Proserpio, Stefania Rapelli, Mara Maldotti, Isabelle Laurence Polignano, Francesca Anselmi, Danny Incarnato, Anna Krepelova, Daniela Donna, Chiara Levra Levron, Giacomo Donati, Ivan Molineris, Francesco Neri, Salvatore Oliviero
Summary: The establishment of DNA methylation patterns during mouse early development is crucial for cell fate determination. However, the specific molecular targets and mechanisms of de novo methylation machinery during differentiation are not fully understood. In this study, the researchers identified DNMT3B-dependent regulatory elements that play a critical role in cell fate determination. They found that DNMT3B-dependent DNA methylation is essential for proper differentiation and lineage determination, and ectopic expression of DNMT3B can restore normal gene expression and cell fate. This study provides important insights into the role of DNA methylation in early development and cell fate determination.
NATURE COMMUNICATIONS
(2023)
Article
Genetics & Heredity
Ryan H. Miller, Chad A. Pollard, Kristin R. Brogaard, Andrew C. Olson, Ryan C. Barney, Larry I. Lipshultz, Erica B. Johnstone, Yetunde O. Ibrahim, James M. Hotaling, Enrique F. Schisterman, Sunni L. Mumford, Kenneth I. Aston, Tim G. Jenkins
Summary: Complex diseases are challenging to diagnose due to their multifactorial etiologies. This study developed a diagnostic tool that analyzes a comprehensive network of epigenetic profiles to identify global shifts associated with complex diseases. The tool was validated by assessing tissue-specific epigenetic variability, differences between diseased and normal tissue, and its utility in male infertility, showing potential for clinical use. This approach expands diagnostic research beyond single genetic targets and provides insights into the role of epigenetic variability in complex diseases.
FRONTIERS IN GENETICS
(2023)
Article
Biotechnology & Applied Microbiology
Ryan J. Corbett, Andrea M. Luttman, Juber Herrera-Uribe, Haibo Liu, Nancy E. Raney, Jenna M. Grabowski, Crystal L. Loving, Christopher K. Tuggle, Catherine W. Ernst
Summary: In this study, whole-genome bisulfite sequencing was performed to assess DNA methylation patterns in pig immune cell populations. The study identified differentially methylated regions (DMRs) and observed correlations between differential methylation and gene expression across immune cell populations. Cell-specific low methylation was found to disproportionately impact genes exhibiting enriched expression in the same cell type. The study also revealed cell type-specific enrichment of transcription factor binding motifs in lowly methylated regions. Furthermore, DMRs were enriched for immune capacity GWAS SNPs, suggesting their importance in immune cell development and function. These findings improve the functional annotation of the porcine genome and enhance the understanding of the links between genotype and phenotype.
Article
Biochemistry & Molecular Biology
Baohua Tan, Chen Zhou, Xupeng Zang, Xinming Zhao, Liyao Xiao, Jiekang Zeng, Linjun Hong, Zhenfang Wu, Ting Gu
Summary: In this study, whole-genome bisulfite sequencing and RNA sequencing were used to study DNA methylation and gene expression changes in Tibetan pig placenta during three stages of development. The results revealed significant changes in morphology and histological structures at the uterine-placental interface. Transcriptome analysis identified differentially expressed genes and revealed key transcriptional properties. The study also found a correlation between DNA methylation level in gene promoters and gene expression, as well as differentially methylated regions associated with placental developmental genes and transcription factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pathology
Yuri Tolkach, Romina Zarbl, Simone Bauer, Manuel Ritter, Joerg Ellinger, Stephan Hauser, Laura Hueser, Sabine M. Klauck, Peter Altevogt, Holger Sultmann, Dimo Dietrich, Glen Kristiansen
Summary: CD24 is overexpressed in many human cancers, including prostate cancer, and its up-regulation can be influenced by DNA methylation of the CD24 promoter. Higher levels of CD24 expression are associated with poorer outcomes in prostate cancer patients, including shorter biochemical recurrence-free survival. Overexpression of ERG and PTEN deficiency are also correlated with increased CD24 expression levels in prostate cancer.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Cell Biology
Samatha Bhat, Shama Prasada Kabekkodu, Divya Adiga, Rayzel Fernandes, Vaibhav Shukla, Poonam Bhandari, Deeksha Pandey, Krishna Sharan, Kapaettu Satyamoorthy
Summary: Cervical cancer is a major cause of cancer-related deaths in developing countries, with zinc-finger protein 471 (ZNF471) identified as a potential tumor suppressor through its epigenetic regulation and targeting of epithelial-mesenchymal transition signaling pathways. High methylation of ZNF471 and low expression are associated with poor prognosis, and ZNF471 overexpression inhibits tumor growth and key signaling pathways in vitro and in vivo.
CELL BIOLOGY AND TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
Rose Ghemrawi, Aya Al Qassem, Azza Ramadan, Raghad Aldulaymi, Nour Sammani, Walaa K. Mousa, Mostafa Khair
Summary: Protein and DNA methylation play important roles in various biological functions. Abnormal regulation of methyltransferases has been implicated in cancer, but its association with autophagy and carcinogenesis in breast and lung cancer is not well understood. In this study, we used UALCAN to analyze breast and lung cancer databases and found that 17 methyltransferases are upregulated in these cancers. We investigated the effects of methylation inhibition on breast and lung cancer cell lines and found that inhibiting methylation reduced cell migration, growth, and autophagy activity. This suggests that targeting methylation ability could be a potential treatment for breast and lung cancer.
Article
Genetics & Heredity
Haihua Xing, Furui Zhang, Ruobing Han, Heping Li
Summary: The biological role of the OPN gene in sika deer antler growth was explored by analyzing different tissues at different growth periods. The methylation level of the OPN promoter decreased as the antler grew, and it was negatively correlated with mRNA expression level.
Article
Oncology
Theophilos Tzaridis, Niklas Schaefer, Johannes Weller, Joachim-Peter Steinbach, Uwe Schlegel, Sabine Seidel, Michael Sabel, Peter Hau, Clemens Seidel, Dietmar Krex, Roland Goldbrunner, Joerg-Christian Tonn, Oliver Grauer, Sied Kebir, Matthias Schneider, Christina Schaub, Hartmut Vatter, Christoph Coch, Martin Glas, Rolf Fimmers, Torsten Pietsch, Guido Reifenberger, Ulrich Herrlinger, Joerg Felsberg
Summary: The CeTeG/NOA-09 trial demonstrated a survival benefit for glioblastoma patients with MGMT promoter methylation ratios >2 who received combined CCNU/TMZ therapy. The prognostic value of qMSP ratio, as well as the concordance of MGMT methylation results obtained by different methods, were evaluated. Different qMSP ratio cutoffs may impact treatment efficacy, with lower ratios potentially showing larger benefits from CCNU/TMZ therapy. Acceptable concordance rates were observed among different MGMT methylation assays, allowing for potential consideration of alternative methods for therapeutic decision-making.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Agriculture, Dairy & Animal Science
W. H. Dong, C. H. Dai, L. Sun, J. Wang, S. Y. Sun, G. Q. Zhu, S. L. Wu, W. B. Bao
Article
Genetics & Heredity
Chaohui Dai, Haifei Wang, Guoqiang Zhu, Shenglong Wu, Wenbin Bao
Article
Biochemistry & Molecular Biology
C. H. Dai, J. Y. Wu, C. X. Zhao, L. H. Yu, W. B. Bao, S. L. Wu
GENETICS AND MOLECULAR RESEARCH
(2017)
Article
Biotechnology & Applied Microbiology
Chaohui Dai, Li Sun, Riwei Xia, Shouyong Sun, Guoqiang Zhu, Shenglong Wu, Wenbin Bao
Article
Biochemistry & Molecular Biology
Li Sun, Sen Wu, Chao-Hui Dai, Shou-Yong Sun, Guo-Qiang Zhu, Sheng-Long Wu, Wen-Bin Bao
BIOSCIENCE REPORTS
(2018)
Article
Agriculture, Dairy & Animal Science
Chaohui Dai, Li Yang, Jian Jin, Haifei Wang, Shenglong Wu, Wenbin Bao
Article
Genetics & Heredity
Chao-Hui Dai, Fang Wang, Shi-Qin Wang, Zheng-Chang Wu, Sheng-Long Wu, Wen-Bin Bao
Article
Agriculture, Multidisciplinary
Dai Chao-hui, Cao Yue, Gao Zhong-cheng, Zhu Guo-qiang, Wu Sheng-long, Bao Wen-bin
JOURNAL OF INTEGRATIVE AGRICULTURE
(2020)
Article
Biochemistry & Molecular Biology
Chao-Hui Dai, Zhong-Cheng Gao, Jin-Hua Cheng, Li Yang, Zheng-Chang Wu, Sheng-Long Wu, Wen-Bin Bao
Summary: This study revealed the expression profiles of miRNAs, mRNAs, lncRNAs, and circRNAs in porcine alveolar macrophage cells infected with H1N1 and H3N2 swine influenza viruses. Two ceRNAs, TCONS_00166432-miR10391-MAN2A1 and novel_circ_0004733-miR10391-MAN2A1, were identified to regulate H1N1 and H3N2 infections. The study provided new insights into the molecular mechanism of 3D4/21 cells against swine influenza virus infection by reporting the ceRNA networks involved in the regulation of H1N1 and H3N2 infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Joan K. Lunney, Angelica Van Goor, Kristen E. Walker, Taylor Hailstock, Jasmine Franklin, Chaohui Dai
Summary: Pigs have significant potential as biomedical models for studying human developmental processes, congenital diseases, and pathogen response mechanisms, as well as serving as xenotransplant organ donors and tools for vaccine and drug design. The similarities between pigs and humans in anatomy, physiology, immunology, and genetics enhance their potential as human models. Therefore, utilizing animal models that closely resemble humans, such as pigs, is crucial for relevant and reproducible research.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Qiuli Tong, Hu Liu, Qianqian Qi, Chaohui Dai, Teddy Yang, Feng Qian
Summary: Glucocorticoid-induced TNF receptor-related (GITR) can be targeted to enhance immunotherapy efficacy by activating T cells and reducing regulatory T cells. In this study, a panel of fully human antibodies targeting GITR was generated and a specific antibody hab019e2 showed enhanced antitumor activity compared to Tab9H6V3. This antibody could be a potential candidate for developing novel drugs targeting GITR.
Article
Biochemistry & Molecular Biology
Yingying Gao, Teddy Yang, Hu Liu, Ningning Song, Chaohui Dai, Yu Ding
Summary: CD137 is an inducible T-cell costimulatory receptor with potential anti-tumor activity. The novel CD137 agonistic antibody PE0116 can promote T-cell proliferation and increase cytokine secretion in vitro, and shows strong anti-tumor activity in vivo.
Meeting Abstract
Immunology
Joan K. Lunney, Taylor Hailstock, Chaohui Dai, Jovan Aquino, Shannon Chick, Kristen Walker, Jean N. Manirarora, Renukaradhya J. Gourapura, Yvonne B. Sullivan, Joanna LaBresh
JOURNAL OF IMMUNOLOGY
(2022)
Meeting Abstract
Immunology
Renukaradhya J. Gourapura, Crystal L. Loving, Scott Kenney, Joanna LaBresh, Veerupaxagouda Patil, Kristen Byrne, Kristen Walker, Chaohui Dai, Taylor Hailstock, Joan K. Lunney
JOURNAL OF IMMUNOLOGY
(2021)