Article
Plant Sciences
Rafael Zuccarelli, Marta Rodriguez-Ruiz, Patricia J. Lopes-Oliveira, Grazieli B. Pascoal, Sonia C. S. Andrade, Claudia M. Furlan, Eduardo Purgatto, Jose M. Palma, Francisco J. Corpas, Magdalena Rossi, Luciano Freschi
Summary: The study found that NO has a significant impact on the ripening process of tomato fruit, including regulating ripening-related genes, affecting metabolite content, and limiting ethylene production. Additionally, NO intensified oxidative stress and nitro-oxidative events, impacting the biosynthesis of compounds such as ascorbate, flavonoids, and lycopene.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Agronomy
Ying Liu, Rob E. Schouten, Yury Tikunov, Xinxuan Liu, Richard G. F. Visser, Fei Tan, Arnaud Bovy, Leo F. M. Marcelis
Summary: This study investigated the effect of light intensity and spectrum on anthocyanin accumulation in purple-fruited Capsicum genotypes. The results showed that increasing the blue light fraction can increase anthocyanin levels and delay fruit ripening. Transcriptome analysis also revealed that fruits illuminated with higher blue light fractions expressed fewer genes related to ripening and senescence.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Salvador Gonzalez-Gordo, Maria A. Munoz-Vargas, Jose M. Palma, Francisco J. Corpas
Summary: The study identified 75 CaPOD genes in sweet pepper based on the existing genome, but only 10 genes were found to be active in the fruit transcriptome. Two genes were upregulated during fruit ripening, seven were downregulated, and one gene was unaffected. Nitric oxide treatment triggered the upregulation of two CaPOD genes while the others were unaffected. In vitro experiments showed that CaPOD IV activity was strongly inhibited by peroxynitrite, NO donors, and reducing agents.
Review
Biochemical Research Methods
Jaclyn A. Adaskaveg, Barbara Blanco-Ulate
Summary: Fruit quality directly affects fruit marketability and consumer acceptance. Breeders have focused on extending shelf life through fruit texture but neglected other qualities such as flavor and nutrition. Integrative biotechnology and consumer-minded approaches have emerged to develop flavorful, long-lasting fruit by targeting specific transcription factors and hormones involved in fruit ripening. Overall, addressing grower and industry needs must be balanced with consumer-based traits.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Salvador Gonzalez-Gordo, Marta Rodriguez-Ruiz, Alberto Paradela, Antonio Ramos-Fernandez, Francisco J. Corpas, Jose M. Palma
Summary: The physiological process of fruit ripening is closely related to the role of mitochondrial proteome, especially in mitochondrial electron transport chain and tricarboxylic acid cycle. Proteomic analysis revealed the key genes and pathways involved in fruit ripening. Moreover, the synthesis of antioxidant ascorbate in the mitochondrial proteome also plays a crucial role in fruit ripening.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Charlotte Steelheart, Matias L. Alegre, Pierre Baldet, Christophe Rothan, Cecile Bres, Daniel Just, Yoshihiro Okabe, Hiroshi Ezura, Inti M. Ganganelli, Gustavo E. Gergoff Grozeff, Carlos G. Bartoli
Summary: This study investigated the role of H2O2 in tomato ripening and found that H2O2 participates in this process through its association with the ethylene signaling pathway. The experiments showed that high irradiance treatment enhanced H2O2 production and accelerated fruit ripening. These results may be attributed to changes in the expression of H2O2-related genes and ethylene-related genes.
Article
Plant Sciences
Ju Hui Do, Seo Young Park, Se Hee Park, Hyun Min Kim, Sang Hoon Ma, Thanh Dat Mai, Jae Sung Shim, Young Hee Joung
Summary: Ascorbate is an essential antioxidant for humans, and increasing its content in tomato fruits can improve their nutritional value. The gene SlAPX4 is specifically induced during fruit ripening, and its mutation leads to elevated ascorbate content in ripe tomato fruits without adverse effects on plant growth.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Irene Murgia, Alessia Midali, Sara Cimini, Laura De Gara, Ekaterina Manasherova, Hagai Cohen, Alexis Paucelle, Piero Morandini
Summary: Plants synthesize ascorbate (ASC) via the D-mannose/L-galactose pathway, while animals produce ASC and H2O2 via the UDP-glucose pathway. A study investigated the role of GULLO2, an isoform of Gulono-1,4 gamma-lactone oxidases, in iron (Fe) nutrition in Arabidopsis thaliana. The findings suggest that GULLO2 is involved in ASC synthesis and Fe(III) reduction, which are crucial for Fe transport and suberin biosynthesis in developing seeds.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Helene Roehricht, Jonathan Przybyla-Toscano, Joachim Forner, Clement Boussardon, Olivier Keech, Nicolas Rouhier, Etienne H. Meyer
Summary: This study identified an atypical mitochondrial ferredoxin (mFDX-like) that plays a crucial role in the assembly of complex I and formation of complex I-containing supercomplexes.
Article
Food Science & Technology
Jirarat Kantakhoo, Yoshihiro Imahori
Summary: The study found that treating red sweet pepper fruit with hot water for 1 minute can reduce chilling injury, electrolyte leakage, and weight loss. This treatment activates the ascorbate-glutathione cycle by enhancing antioxidant levels and the activity of related enzymes, ultimately increasing the fruit's tolerance to cold damage.
Article
Plant Sciences
Priya Gambhir, Utkarsh Raghuvanshi, Adwaita Prasad Parida, Stuti Kujur, Shweta Sharma, Sudhir K. Sopory, Rahul Kumar, Arun Kumar Sharma
Summary: Methylglyoxal detoxification, catalyzed by glutathione-dependent glyoxalase I and regulated by the MADS-box transcription factor RIPENING INHIBITOR, is crucial for fruit ripening. The decline of MG levels during fruit ripening is mainly mediated through a glutathione-dependent MG detoxification pathway and primarily catalyzed by a Glyoxalase I enzyme encoded by the SlGLYI4 gene. Silencing of SlGLYI4 leads to drastic MG overaccumulation at ripening stages of transgenic fruits and interferes with the ripening process.
Review
Plant Sciences
Francisco J. Corpas, Marta Rodriguez-Ruiz, Maria A. Munoz-Vargas, Salvador Gonzalez-Gordo, Russel J. Reiter, Jose M. Palma
Summary: Melatonin plays a crucial role in fruit ripening by interacting with nitric oxide and hydrogen peroxide, expanding the regulatory networks of these molecules.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Jorge Taboada, Salvador Gonzalez-Gordo, Maria A. Munoz-Vargas, Jose M. Palma, Francisco J. Corpas
Summary: NADPH is an essential cofactor involved in various physiological processes. In pepper fruits, five NADP-ME genes were identified and four of them were found to be expressed. The expression of these genes was differentially modulated during fruit ripening, with CaNADP-ME3 and CaNADP-ME5 upregulated, and CaNADP-ME2 and CaNADP-ME4 downregulated. Exogenous NO gas treatment triggered the downregulation of CaNADP-ME4. Protein fraction containing CaNADP-ME enzyme activity was obtained and four isozymes were identified.
Article
Biochemistry & Molecular Biology
Salvador Gonzalez-Gordo, Amanda Canas, Maria A. Munoz-Vargas, Jose M. Palma, Francisco J. Corpas
Summary: In this study, the distribution pattern of LOX isozymes in various organs and different pungency levels of sweet pepper fruits was investigated. The results showed that the number of LOX isozymes was positively correlated with the capsaicin content in fruits, and their expression was differentially regulated by fruit ripening and NO treatment. Furthermore, a regulatory mechanism via S-nitrosation was identified for LOX IV isoenzyme activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Salvador Gonzalez-Gordo, Jose M. Palma, Francisco J. Corpas
Summary: The proteomic composition of peroxisomes in sweet pepper fruits was analyzed, revealing significant differences in peroxisomal proteins involved in antioxidant metabolism and beta-oxidation pathway. These findings provide new insights into the metabolic functions of peroxisomes during fruit ripening.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Curro Polo-Castellano, Rosa Maria Mateos, Francisco Visiedo, Miguel Palma, Gerardo F. Barbero, Marta Ferreiro-Gonzalez
Summary: Moringa oleifera Lam. is a tree with significant antioxidant properties, making the development of an optimal extraction method crucial for obtaining pharmacological products based on its bioactive compounds. Enzymatic extraction conditions were optimized using Plackett-Burman and Box-Behnken designs, with normalized areas and relative mass as response and quantification variables, respectively. Under the optimal extraction conditions, enzyme extractions were performed and evaluated for antioxidant power, total phenolic compounds, repeatability, and intermediate precision.
Article
Plant Sciences
Salvador Gonzalez-Gordo, Jose M. Palma, Francisco J. Corpas
Summary: Small heat shock proteins (sHSPs) are upregulated in plants under environmental stresses and act as molecular chaperones to protect other proteins. This study identified 41 sHSP genes in the genome of sweet pepper fruits, with 19 genes found in previous transcriptome data. The expression analysis during fruit ripening showed that 6 genes were upregulated, 7 genes were downregulated, and 6 genes were unchanged. In addition, nitric oxide treatment caused the upregulation of 7 genes, downregulation of 3 genes, and no change in 9 genes. These findings indicate the role of sHSPs in pepper fruit ripening and nitro-oxidative stress.
Meeting Abstract
Biochemistry & Molecular Biology
Marta Rodriguez-Ruiz, Carmen Ramos, Maria Jesus Campos, Francisca Vicente, Francisco J. Corpas, Jose M. Palma
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Jorge Taboada, Salvador Gonzalez-Gordo, Jose M. Palma, Francisco J. Corpas
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Salvador Gonzalez-Gordo, Javier Lopez-Jaramillo, Jose M. Palma, Francisco J. Corpas
Summary: Hydrogen sulfide (H2S) and nitric oxide (NO) negatively affect the activity of soybean lipoxygenase type 1 (LOX 1), potentially through S-nitrosation and persulfidation. Among the thirty-five tyrosine residues present in LOX 1, only Y214 was exclusively nitrated by peroxynitrite (ONOO-), thereby affecting its interaction with W500 in the substrate binding site. Analysis of the protein structure reveals the existence of tunnels connecting the protein surface with internal cysteine residues, facilitating persulfidation (especially C429 and C127) and nitrosation (particularly of C127) by H2S and the hydrophilic, bulkier CysNO molecule, respectively.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Rafael Zuccarelli, Marta Rodriguez-Ruiz, Fernanda O. Silva, Leticia D. L. Gomes, Patricia J. Lopes-Oliveira, Agustin Zsogon, Sonia C. S. Andrade, Diego Demarco, Francisco J. Corpas, Lazaro E. P. Peres, Magdalena Rossi, Luciano Freschi
Summary: Research shows that endogenous nitric oxide (NO) plays a regulatory role in tomato shoot architecture, fruit set, and growth. Inhibiting S-nitrosoglutathione reductase (GSNOR) in tomato promotes shoot side branching and reduces fruit size, negatively impacting fruit yield. It was also found that GSNOR inhibition results in increased protein tyrosine nitration and S-nitrosation, affecting auxin production and signaling in leaf primordia and developing ovaries of fruits.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Endocrinology & Metabolism
Francisco Visiedo, Luis Vazquez-Fonseca, Jessica Abalos-Martinez, J. Roman Broullon-Molanes, Rocio Quintero-Prado, Rosa Maria Mateos, Fernando Bugatto
Summary: This study investigated the impact of maternal circulating inflammatory mediators on placental fatty acid metabolism in women with gestational diabetes mellitus (GDM). The results showed that GDM patients had higher levels of inflammatory cytokines and lower fatty acid oxidation capacity in the placenta, along with increased triglyceride levels. The findings suggest that the maternal inflammatory status may interfere with the delivery of maternal fat to the fetus via the placenta.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Salvador Gonzalez-Gordo, Maria A. Munoz-Vargas, Jose M. Palma, Francisco J. Corpas
Summary: The study identified 75 CaPOD genes in sweet pepper based on the existing genome, but only 10 genes were found to be active in the fruit transcriptome. Two genes were upregulated during fruit ripening, seven were downregulated, and one gene was unaffected. Nitric oxide treatment triggered the upregulation of two CaPOD genes while the others were unaffected. In vitro experiments showed that CaPOD IV activity was strongly inhibited by peroxynitrite, NO donors, and reducing agents.
Article
Plant Sciences
Jorge Taboada, Salvador Gonzalez-Gordo, Maria A. Munoz-Vargas, Jose M. Palma, Francisco J. Corpas
Summary: NADPH is an essential cofactor involved in various physiological processes. In pepper fruits, five NADP-ME genes were identified and four of them were found to be expressed. The expression of these genes was differentially modulated during fruit ripening, with CaNADP-ME3 and CaNADP-ME5 upregulated, and CaNADP-ME2 and CaNADP-ME4 downregulated. Exogenous NO gas treatment triggered the downregulation of CaNADP-ME4. Protein fraction containing CaNADP-ME enzyme activity was obtained and four isozymes were identified.
Article
Medicine, Research & Experimental
Alvaro Gonzalez-Dominguez, Thalia Belmonte, Jesus Dominguez-Riscart, Pablo Ruiz-Ocana, Ines Muela-Zarzuela, Ana Saez-Benito, Raul Montanez-Martinez, Rosa M. M. Mateos, Alfonso M. M. Lechuga-Sancho
Summary: This study found that insulin response is associated with oxidative stress and inflammation in children, and children with impaired insulin response have worse lipid and carbohydrate metabolism and are more likely to develop type 2 diabetes. Uric acid may mediate pathological inflammatory response by activating NLRP3 in peripheral blood mononuclear cells.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Marta Rodriguez-Ruiz, Maria C. Ramos, Maria J. Campos, Inmaculada Diaz-Sanchez, Bastien Cautain, Thomas A. A. Mackenzie, Francisca Vicente, Francisco J. J. Corpas, Jose M. Palma
Summary: Cancer, characterized by an alteration of the oxidative metabolism, is a major cause of human death worldwide. Many natural compounds from plant origin with anti-tumor attributes have been described, including capsaicin, the molecule responsible for the pungency in hot pepper fruits, which has shown anti-proliferative properties against cancer. The study found that the capsaicin content in pepper fruits did not correspond with their anti-proliferative activity, indicating the presence of other compounds with anti-tumor potentiality in pepper fruits.
Article
Plant Sciences
Maria A. Munoz-Vargas, Salvador Gonzalez-Gordo, Jorge Taboada, Jose M. Palma, Francisco J. Corpas
Summary: This study investigated the transcriptomic, molecular, and functional levels of the G6PDH system in pepper fruit during ripening. The results showed that different CaG6PDH genes were differentially regulated during different ripening stages. Exogenous treatment with NO gas downregulated CaG6PDH2, while positively regulating other genes. In-gel analysis identified two isozymes, CaG6PDH I and CaG6PDH II. The study provides insights into the modulation of CaG6PDHs at the gene and activity levels in pepper fruit during ripening and after NO post-harvest treatment.