Article
Microbiology
Farzaneh Hassanshahi, Mojgan Noroozi Karimabad, Elahe Miranzadeh, Gholamhossein Hassanshahi, Seyedeh Atekeh Torabizadeh, Ali Jebali
Summary: The study investigated the association between Brucella infection and the chemokines CXCL9, 10, and 11. The results showed that the serum levels of these chemokines were significantly increased in patients with acute brucellosis, suggesting their potential as markers for the disease.
CURRENT MICROBIOLOGY
(2023)
Article
Immunology
Wout de Mey, Hanne Locy, Kirsten De Ridder, Phaedra De Schrijver, Dorien Autaers, Asma Lakdimi, Arthur Esprit, Lorenzo Franceschini, Kris Thielemans, Magali Verdonck, Karine Breckpot
Summary: The maturation of dendritic cells (DCs) using TriMix mRNA can induce an antibacterial transcriptional program, while the use of TetraMix mRNA can redirect DCs into an antiviral transcriptional program and induce tumor antigen-specific T cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ildefonso Sanchez-Cerrillo, Diego Calzada-Fraile, Ana Triguero-Martinez, Marta Calvet-Mirabent, Olga Popova, Cristina Delgado-Arevalo, Mariel Valdivia-Mazeyra, Marta Ramirez-Huesca, Enrique Vazquez de Luis, Alberto Benguria, Teresa Acena-Gonzalo, Roberto Moreno-Vellisca, Magdalena Adrados de Llano, Hortensia de la Fuente, Ilya Tsukalov, Pablo Delgado-Wicke, Elena Fernandez-Ruiz, Emilia Roy-Vallejo, Reyes Tejedor-Lazaro, Almudena Ramiro, Salvador Iborra, Francisco Sanchez-Madrid, Ana Dopazo, Isidoro Gonzalez Alvaro, Santos Castaneda, Enrique Martin-Gayo
Summary: This study reveals the increased proportion of CD16(+) CD56hi NK cell subset and CD64(+) conventional dendritic cells (cDC2) in pSS patients, suggesting their involvement in the progression of the disease. The cDC2 can activate NK cells ex vivo and are found in proximity to NK cells in the salivary glands. The expression of NKG2D ligands on cDC2 from pSS patients is regulated by the IFN signatures associated with the RIG-I/DDX60 pathway. Injection of poly I:C leads to increased proportions of CD64hi RAE-1(+) cDC2 and NKG2D(+) CD11b(+) CD27(+) NK cells in the salivary glands.
Article
Dermatology
Tangxiele Liu, Ji Li, Zhili Deng, Mengting Chen, Ke Sha, Wenqin Xiao, Hongfu Xie, Zhixiang Zhao
Summary: This study examined the serum levels of various chemokines in rosacea patients and found that the concentrations of CCL3, CXCL8, CXCL9, and CXCL10 were significantly increased. The levels of these chemokines correlated with the severity of rosacea. The expression of their corresponding receptors was also elevated in the skin lesions of rosacea patients and mouse model. These findings may contribute to the clinical diagnosis and treatment of rosacea.
JOURNAL OF DERMATOLOGY
(2022)
Article
Cell Biology
Paolo Strati, Xubin Li, Qing Deng, Mario L. Marques-Piubelli, Jared Henderson, Grace Watson, Laurel Deaton, Taylor Cain, Haopeng Yang, Vida Ravanmehr, Luis E. Fayad, Swaminathan P. Iyer, Loretta J. Nastoupil, Frederick B. Hagemeister, Edwin R. Parra, Neeraj Saini, Koichi Takahashi, Nathan H. Fowler, Jason R. Westin, Raphael E. Steiner, Ranjit Nair, Christopher R. Flowers, Linghua Wang, Sairah Ahmed, Gheath Al-Atrash, Francisco Vega, Sattva S. Neelapu, Michael R. Green
Summary: Autologous anti-CD19 CAR T therapy shows high effectiveness in rrLBCL, but its toxicities result in delayed recovery. The pathophysiology of prolonged cytopenia after CAR T infusion is poorly understood. Single-cell RNA sequencing reveals that clonally expanded CX3CR1(hi) cytotoxic T cells with high IFN-g and cytokine signaling are associated with prolonged cytopenia, suggesting a potential mechanism-based approach for its treatment.
CELL REPORTS MEDICINE
(2023)
Article
Multidisciplinary Sciences
Pavithra Sampath, Anuradha Rajamanickam, Kannan Thiruvengadam, Alangudi Palaniappan Natarajan, Syed Hissar, Madhavan Dhanapal, Bharathiraja Thangavelu, Lavanya Jayabal, Paranchi Murugesan Ramesh, Uma Devi Ranganathan, Subash Babu, Ramalingam Bethunaickan
Summary: Diagnosing tuberculosis (TB) continues to be a challenge, especially with current immune-based diagnostic methods such as Interferon Gamma Release Assay. Chemokines, which play a role in immune responses, are considered important markers for defining the disease status of TB. In this study, the levels of chemokines were examined in individuals with drug-resistant, drug-sensitive, and latent TB compared to healthy individuals. The results showed differential levels of chemokines between the study groups, with CXCL10 and CXCL9 identified as potential markers for drug-resistant and drug-sensitive TB.
SCIENTIFIC REPORTS
(2023)
Article
Gastroenterology & Hepatology
Shuai Wang, Qinchuan Wu, Tianchi Chen, Rong Su, Caixu Pan, Junjie Qian, Hechen Huang, Shengyong Yin, Haiyang Xie, Lin Zhou, Shusen Zheng
Summary: In addition to the classical DC-T cell axis, CD47 blockade significantly enhances the ability of CD103(+) DCs to take up tumor DNA, resulting in the stimulation of the cGAS-STING pathway and the promotion of NK cell infiltration and activation in liver cancer.
JOURNAL OF HEPATOLOGY
(2022)
Article
Oncology
Romain Banchereau, Avantika S. Chitre, Alexis Scherl, Thomas D. Wu, Namrata S. Patil, Patricia de Almeida, Edward E. Kadel, Shravan Madireddi, Amelia Au-Yeung, Chikara Takahashi, Ying-Jiun Chen, Zora Modrusan, Jacqueline McBride, Rhea Nersesian, Ehab A. El-Gabry, Mark D. Robida, Jeffrey C. Hung, Marcin Kowanetz, Wei Zou, Mark McCleland, Patrick Caplazi, Shadi Toghi Eshgi, Hartmut Koeppen, Priti S. Hegde, Ira Mellman, W. Rodney Mathews, Thomas Powles, Sanjeev Mariathasan, Jane Grogan, William E. O'Gorman
Summary: CD8+ tissue-resident memory T (T-RM) cells expressing CD103 (ITGAE) are believed to actively suppress cancer progression, and their presence in tumors may predict response to immunotherapy. This study found that in inflamed tumors, CD103+ CD8+ T-RM cells exhibit a complex phenotype characterized by increased clonal expansion, expression of checkpoint regulators, and cytotoxic proteins, suggesting their involvement in antitumor response.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Oncology
Snehanshu Chowdhury, Anwesha Kar, Debaleena Bhowmik, Anupam Gautam, Debashree Basak, Ishita Sarkar, Puspendu Ghosh, Deborpita Sarkar, Alvina Deka, Paramita Chakraborty, Asima Mukhopadhyay, Shikhar Mehrotra, Soumen Basak, Sandip Paul, Shilpak Chatterjee
Summary: IL-12 stimulation increases intracellular acetyl CoA levels in CD8(+) T cells, allowing them to maintain effector function in nutrient-deprived tumor microenvironments. Inhibition of ATP-citrate lyase, an enzyme involved in the glucose-citrate-acetyl CoA pathway, impairs IFN gamma production and viability of CD8(+) T cells in nutrient-restricted conditions. CD8(+) T cells cultured in high pyruvate-containing media acquire metabolic features of IL-12-stimulated CD8(+) T cells and display improved antitumor potential.
Article
Cell Biology
A. Helena Jonsson, Fan Zhang, Garrett Dunlap, Emma Gomez-Rivas, Gerald F. M. Watts, Heather J. Faust, Karishma Vijay Rupani, Joseph R. Mears, Nida Meednu, Runci Wang, Gregory Keras, Jonathan S. Coblyn, Elena M. Massarotti, Derrick J. Todd, Jennifer H. Anolik, Andrew McDavid, Kevin Wei, Deepak A. Rao, Soumya Raychaudhuri, Michael B. Brenner
Summary: CD8 T cells play a significant role in the pathogenesis of rheumatoid arthritis by producing pro-inflammatory cytokines. These CD8 T cells are abundant in synovial tissue and belong to an effector population characterized by high expression of granzyme K and low expression of granzyme B and perforin. They are clonally expanded in synovial tissues and maintain their characteristics in blood, suggesting their enrichment in tissues. They also exist in other tissues and diseases, indicating their role as tissue-associated T cells.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
Ella Bhagyaraj, Hongbin Wang, Xinghong Yang, Carol Hoffman, Ali Akgul, Zakia Goodwin, David W. Pascual
Summary: znBAZ infection induces an increase and activation of lung NK cells in mice, which play a crucial role in promoting lung DC activation, migration, and the development of protective CD8(+) T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Dermatology
Pawit Phadungsaksawasdi, Toshiharu Fujiyama, Kazuo Kurihara, Taisuke Ito, Tetsuya Honda, Yoshiki Tokura
Summary: In human psoriasis, PD-1 expressing CD8(+) CD103(+) T cells in the skin are associated with disease severity and histopathology, indicating a potential pathogenic subset that may play a role in psoriasis pathogenesis.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Oncology
Hemavathi Dhandapani, Hascitha Jayakumar, Abirami Seetharaman, Shirley Sunder Singh, Selvaluxmy Ganeshrajah, Nirmala Jagadish, Anil Suri, Rajkumar Thangarajan, Priya Ramanathan
Summary: Dendritic cell-based immunotherapy utilizing recombinant human SPAG9 shows promise in stimulating CD4(+) and CD8(+) T cell responses for cervical cancer patients. The study suggests rhSPAG9 as a strong immunogen for DC-based immunotherapy and highlights the potential of a combinatorial regimen with cisplatin for therapeutic intervention.
CANCER CELL INTERNATIONAL
(2021)
Article
Microbiology
Tej Pratap Singh, Augusto M. Carvalho, Lais Amorim Sacramento, Elizabeth A. Grice, Phillip Scott
Summary: This study demonstrates the involvement of IL-17A-producing ILCs in microbiota-driven immunopathology in cutaneous leishmaniasis. Skin microbiota promotes the generation of ROR gamma t(+) IL-17A-producing ILCs, which exacerbate skin inflammation in the disease.
Article
Cell Biology
Mallory Paynich Murray, Catherine M. Crosby, Paola Marcovecchio, Nadine Hartmann, Shilpi Chandra, Meng Zhao, Archana Khurana, Sonja P. Zahner, Bjorn E. Clausen, Fadie T. Coleman, Joseph P. Mizgerd, Zbigniew Mikulski, Mitchell Kronenberg
Summary: This study investigates the roles of innate-like T cells during pulmonary pneumococcal infection. The researchers found that these T cells rapidly increase in the lung tissue following infection and participate in the immune response. Specifically, interleukin-17-producing NKT17 and gamma delta T cells play a major role in the infection, with NKT17 cells being preferentially located in lung tissue prior to infection and interacting with CD103(+) dendritic cells. These studies demonstrate the importance of specific cellular interactions and functional subsets of innate-like T cells in protecting against lung infection.
Article
Oncology
Shih-Chung Wang, Kuender D. Yang, Ching-Yuang Lin, Alex Y. Huang, Chien-Chou Hsiao, Ming-Tsan Lin, Yi-Giien Tsai
PEDIATRIC BLOOD & CANCER
(2020)
Article
Biochemistry & Molecular Biology
Stefania De Santis, Giulio Verna, Grazia Serino, Raffaele Armentano, Elisabetta Cavalcanti, Marina Liso, Manuela Dicarlo, Sergio Coletta, Mauro Mastronardi, Antonio Lippolis, Angela Tafaro, Angelo Santino, Aldo Pinto, Pietro Campiglia, Alex Y. Huang, Fabio Cominelli, Theresa T. Pizarro, Marcello Chieppa
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Chemistry, Analytical
Deborah S. Barkauskas, Gregory Medley, Xiaowen Liang, Yousuf H. Mohammed, Camilla A. Thorling, Haolu Wang, Michael S. Roberts
METHODS AND APPLICATIONS IN FLUORESCENCE
(2020)
Article
Oncology
Lei Wang, Shuiliang Yu, Ernest R. Chan, Kai-Yuan Chen, Cui Liu, Danian Che, Amad Awadallah, Jay Myers, David Askew, Alex Y. Huang, Ivan Maillard, Dan Huang, Wei Xin, Lan Zhou
Summary: The study found that Notch2 signaling plays an important role in cDC1-mediated antitumor immunity by regulating DC terminal differentiation, CCR7 expression, and antigen presentation, leading to inhibition of inflammation-associated colon cancer development. Suppressed cDC1 gene signature expression in human colorectal cancer is associated with poor prognosis.
CANCER IMMUNOLOGY RESEARCH
(2021)
Meeting Abstract
Oncology
Katherine Daunov, Michael Daunov, Kara Noskoff, Hilary Gan, Simon Davies, Olivia Larbi, Megan Farrell, Whitney Hadley, Amelia Renee Baffa, Jennifer Giesel, Rachel A. Egler, Alex Yee-Chen Huang, John J. Letterio, Richard T. Lee
JOURNAL OF CLINICAL ONCOLOGY
(2021)
Article
Oncology
Sarah B. Whittle, Katharine Offer, Ryan D. Roberts, Amy LeBlanc, Cheryl London, Robbie G. Majzner, Alex Y. Huang, Peter Houghton, E. Alejandro Sweet Cordero, Patrick J. Grohar, Michael Isakoff, Michael W. Bishop, Elizabeth Stewart, Emily K. Slotkin, Emily Greengard, Scott C. Borinstein, Fariba Navid, Richard Gorlick, Katherine A. Janeway, Damon R. Reed, Pooja Hingorani
Summary: Osteosarcoma is the most common bone tumor in children and young adults with poor prognosis for metastatic and relapsed disease. The Children's Oncology Group Bone Tumor Committee established a task force to prioritize agents for clinical trials, identifying multitargeted tyrosine kinase inhibitors as the top agents of interest. Only these inhibitors met all criteria for frontline evaluation and have been incorporated into an upcoming phase III study concept. The task force will continue to reassess identified agents and evaluate novel agents using this method as new data become available.
PEDIATRIC BLOOD & CANCER
(2021)
Article
Multidisciplinary Sciences
Jeong-su Do, Daniel Zwick, Jonathan D. Kenyon, Fei Zhong, David Askew, Alex Y. Huang, Wouter Van't Hof, Marcie Finney, Mary J. Laughlin
Summary: Co-culturing human iTregs with MSCs enhances the stability of FOXP3 expression and suppressive function, with a key mechanism being MSC mt transfer via tunneling nanotubules. Mitochondrial metabolic function drives MSC mt transfer to proliferating iTregs, stabilizing FOXP3 expression and promoting suppressive function in inflammatory conditions.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Byung-Gyu Kim, Sung Hee Choi, John J. Letterio, Jie-Young Song, Alex Y. Huang
Summary: The study demonstrates that overexpression of VEGF in plasmacytoma inhibits tumor growth and enhances T-cell-mediated antitumor immune response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Lalitha Nayak, David R. Sweet, Asha Thomas, Stephanie D. Lapping, Kenneth Kalikasingh, Annmarie Madera, Vinesh Vinayachandran, Roshan Padmanabhan, Neelakantan T. Vasudevan, Jay T. Myers, Alex Y. Huang, Alvin Schmaier, Nigel Mackman, Xudong Liao, Andrei Maiseyeu, Mukesh K. Jain
Summary: Arterial and venous thrombosis are major global diseases with common mechanisms involving neutrophils. This study identified neutrophils as key effectors in thrombosis and demonstrated the feasibility of targeting them using immunoregulatory nanoparticles. Moreover, key molecular events and regulators of neutrophil activation were identified, providing potential targets for therapeutics against immunothrombosis.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Review
Cell Biology
Kristen A. A. VanHeyst, Sung Hee Choi, Daniel T. T. Kingsley, Alex Y. Y. Huang
Summary: Vascular Cell Adhesion Molecule-1 (VCAM-1) is a membrane protein with critical physiological functions in cellular immune response. It can be cleaved into a soluble form (sVCAM-1) and elevated concentrations of sVCAM-1 have been found in the peripheral blood of cancer patients. The VCAM-1/VLA-4 signaling plays a vital role in tumor metastasis and therapy resistance.
Article
Oncology
Veronique Roche, Victor Sandoval, Claire Wolford, Zachary Senders, Julian Anthony Kim, Susan Pereira Ribeiro, Alex Yicheng Huang, Rafick-Pierre Sekaly, Joshua Lyons, Mei Zhang
Summary: This study found that the carbohydrate ligand BG34-200 can bind to activated CD11b, resulting in significant effects on tumor-associated inflammatory monocytes (TAIMs) in three solid tumor backgrounds (osteosarcoma, advanced melanoma, and PDAC). This binding triggers structural biological changes in TAIMs, inducing F-actin cytoskeletal rearrangement, effective phagocytosis, and ICAM-1 clustering. These changes lead to the differentiation of TAIMs into monocyte-derived dendritic cells, enhancing T-cell activation in the tumor microenvironment and potentially improving immunotherapy for solid cancers.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Oncology
Mohammad Alshebremi, Suzanne L. Tomchuck, Jay T. Myers, Daniel T. Kingsley, Saada Eid, Muta Abiff, Melissa Bonner, Shahrazad T. Saab, Sung Hee Choi, Alex Yee-Chen Huang
Summary: The tumor cell-intrinsic STING pathway plays a crucial role in the effectiveness of cryoablation, and the expression of STING-related signaling components may serve as a potential therapy response biomarker.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Meeting Abstract
Oncology
Mohammad Abdulaziz Alshebremi, Jay T. Myers, Daniel T. Kingsley, Alex Y. Huang
Meeting Abstract
Immunology
Muta Abiff, Daniel Kingsley, Peter Rauhe, Bryan Benson, Agne Petrosiute, Alex Yee-Chen Huang
JOURNAL OF IMMUNOLOGY
(2020)
Meeting Abstract
Gastroenterology & Hepatology
Lei Wang, Shuiliang Yu, Cui Liu, Weihuan Wang, Danian Che, Luoyi Li, Jay Myers, David Askew, Ernest R. Chan, Alex Huang, Ivan Maillard, Ivaylo Ivanov, Wei Xin, Lan Zhou