Article
Medicine, General & Internal
Ikhwan Rinaldi, Mondastri Korib Sudaryo, Nurhayati Adnan Prihartono
Summary: This study found that the prevalence of DIC in septic patients was 20.2%. Low albumin, respiratory infection, and antibiotic treatment >= 1 h were identified as risk factors for the development of DIC in septic patients.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Hematology
Kasper Adelborg, Julie B. Larsen, Anne-Mette Hvas
Summary: Disseminated intravascular coagulation (DIC) is a systemic activation of the coagulation system, leading to microvascular thrombosis and hemorrhage. Underlying conditions like infection, cancer, or obstetrical complications initiate and propagate the DIC process. Timely and accurate diagnosis and treatment of DIC and its underlying causes are essential for prognosis.
BRITISH JOURNAL OF HAEMATOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ramona D'Amico, Francesco Monaco, Rosalba Siracusa, Marika Cordaro, Roberta Fusco, Alessio Filippo Peritore, Enrico Gugliandolo, Rosalia Crupi, Salvatore Cuzzocrea, Rosanna Di Paola, Daniela Impellizzeri, Tiziana Genovese
Summary: The study found that um-PEA can reduce changes in coagulation markers and proinflammatory cytokine release caused by LPS, as well as prevent the formation of fibrin deposition and lung damage. Furthermore, um-PEA can also reduce the number of mast cells and the release of serine proteases, which are necessary for SARS-CoV-2 infection. These results suggest that um-PEA could be a potential therapeutic approach in managing DIC and associated clinical implications of coagulopathy and lung dysfunction, such as COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Ivanka Jimenez-Dinamarca, Yolanda Prado, Pablo Tapia, Sebastian Gatica, Clemens Alt, Charles P. Lin, Cristian Reyes-Martinez, Carmen G. Feijoo, Cristobal Aravena, Alejandra Gonzalez-Canacer, Simon Correa, Diego Varela, Claudio Cabello-Verrugio, Felipe Simon
Summary: This study found that TRPM7 in endothelial cells regulates coagulation during sepsis, contributing to inflammation and increased mortality. Additionally, TRPM7 expression in circulating endothelial cells accurately predicts mortality in septic shock patients, making it a potential prognostic biomarker and target for treating sepsis-related coagulation disorders.
BIOLOGICAL RESEARCH
(2023)
Review
Medicine, General & Internal
Junjun Wu, Huaming Li, Yufang Wang, Rong Xu
Summary: This case report highlights the importance of early diagnosis of disseminated intravascular coagulation (DIC) and reviews the clinical indicators of E coli infection and DIC. The patient, a 48-year-old man with symptoms of diarrhea, nausea, vomiting, fever, and loss of consciousness, was diagnosed with acute gastroenteritis complicated by DIC. Timely assessment of platelet count, procalcitonin level, coagulation function, and rational use of antibiotics can improve the prognosis of patients.
Review
Biochemistry & Molecular Biology
Irene Canovas-Cervera, Elena Nacher-Sendra, Rebeca Osca-Verdegal, Enric Dolz-Andres, Jesus Beltran-Garcia, Maria Rodriguez-Gimillo, Carolina Ferrando-Sanchez, Nieves Carbonell, Jose Luis Garcia-Gimenez
Summary: Disseminated Intravascular Coagulation (DIC) is a type of tissue and organ dysregulation in sepsis, with underlying molecular mechanisms that are not fully understood. Current biomarkers for DIC provide poor diagnosis and prognosis, thus non-coding RNAs are studied as potential biomarkers. This review focuses on the relationship between non-coding RNAs and DIC, particularly the PI3K/AKT/mTOR axis, and identifies several ncRNAs involved in its regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Critical Care Medicine
Masaharu Imaura, Fumihiko Katagiri, Satoshi Nagase, Saori Hatoyama-Tanaka, Hiroyuki Takahashi, Risa Takayanagi, Hiroshi Kanno, Yasuhiko Yamada
Summary: The optimal therapeutic plasma concentration of TM alfa for treating DIC associated with sepsis has been determined to be 1,010 ng/mL, based on the cutoff value calculated in this study. Patients with a plasma trough concentration above this cutoff value showed a significantly higher 90-day survival rate compared to those below the cutoff value.
Article
Biochemistry & Molecular Biology
Yan Zhang, Congqing Wu, Lan Li, Ankit Pandeya, Guoying Zhang, Jian Cui, Daniel Kirchhofer, Jeremy P. Wood, Susan S. Smyth, Yinan Wei, Zhenyu Li
Summary: Histones can trigger coagulation activation, and lytic cell death is a novel mechanism of histone-induced coagulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Tadashi Matsuoka, Kazuma Yamakawa, Toshiaki Iba, Koichiro Homma, Junichi Sasaki
Summary: In septic DIC, individuals with a high risk of death, persistent DIC, and late-onset DIC are poor-prognostic subgroups. However, high-risk DIC is also a subgroup that can obtain more benefits from anticoagulant therapy.
THROMBOSIS AND HAEMOSTASIS
(2023)
Review
Cell Biology
Ahsanullah Unar, Lorenzo Bertolino, Fabian Patauner, Raffaella Gallo, Emanuele Durante-Mangoni
Summary: Sepsis is a global health problem caused by an uncontrolled host response to infection, leading to organ failure. Disseminated intravascular coagulation (DIC) is a complication of sepsis that significantly increases mortality. Currently, there is no definitive treatment for sepsis, but early detection and treatment of sepsis-related DIC are important for improving prognosis. This article provides a comprehensive overview of sepsis-related DIC, including its causes and diagnoses, to guide healthcare providers in the care of septic patients.
Review
Hematology
Narcis Popescu, Cristina Lupu, Florea Lupu
Summary: Disseminated intravascular coagulation (DIC) is a syndrome characterized by excessive thrombin generation and widespread fibrinogen conversion triggered by infectious and noninfectious pathologies. Recognizing DIC is critical for reducing the pathological burden and improving patient outcomes.
Review
Critical Care Medicine
Toshiaki Iba, Julie Helms, Jean Marie Connors, Jerrold H. Levy
Summary: The International Society on Thrombosis and Haemostasis (ISTH) released overt DIC diagnostic criteria in 2001. However, DIC includes early stages with systemic activation in coagulation, and thus the ISTH recently released SIC criteria that can diagnose the compensated-phase of coagulopathy.
JOURNAL OF INTENSIVE CARE
(2023)
Article
Medicine, Research & Experimental
Shishuai Meng, Kai Kang, Dongsheng Fei, Songlin Yang, Quankuan Gu, ShangHa Pan, Mingyan Zhao
Summary: In patients with septic DIC, circulating MP levels are positively correlated with DIC scores and negatively correlated with coagulation factors, while TF+-MP activity is not correlated with clotting factor levels and TFPI.
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
(2021)
Review
Hematology
Takeshi Wada, Satoshi Gando
Summary: The two phenotypes of disseminated intravascular coagulation (DIC) are thrombotic and fibrinolytic, characterized by thrombosis and hemorrhage respectively. Thrombotic DIC is caused by coagulation activation, insufficient anticoagulation, and inhibition of fibrinolysis, leading to microvascular fibrin thrombosis and organ dysfunction. Fibrinolytic DIC is characterized by extensive thrombin generation and systemic pathologic hyperfibrinolysis, resulting in severe bleeding.
THROMBOSIS AND HAEMOSTASIS
(2023)
Review
Medicine, General & Internal
Ahsanullah Unar, Lorenzo Bertolino, Fabian Patauner, Raffaella Gallo, Emanuele Durante-Mangoni
Summary: Disseminated intravascular coagulation (DIC) is a significant complication of sepsis. Early diagnosis and effective treatment of sepsis-induced DIC are important for prognosis. However, healthcare providers worldwide still face challenges in treating this condition and further research is needed.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Minori Ishida, Fu Namai, Suguru Shigemori, Shoko Kajikawa, Masami Tsukagoshi, Takashi Sato, Tasuku Ogita, Takeshi Shimosato
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Fu Namai, Suguru Shigemori, Tasuku Ogita, Takashi Sato, Takeshi Shimosato
EXPERIMENTAL AND MOLECULAR MEDICINE
(2020)
Article
Cell Biology
Sayaka Shinji, Koji Umezawa, Yuma Nihashi, Shunichi Nakamura, Takeshi Shimosato, Tomohide Takaya
Summary: This study demonstrates that 18-base telomeric oligodeoxynucleotides designed from the Lactobacillus rhamnosus GG genome promote differentiation of skeletal muscle myoblasts by antagonizing nucleolin and modulating gene expression. The alkaloid berberine enhances the activity of the oligodeoxynucleotides by stabilizing and optimizing their conformation. These bacterial-derived oligodeoxynucleotides serve as aptamers and potential nucleic acid drugs that target myoblasts directly.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Physiology
Shunichi Nakamura, Shinichi Yonekura, Takeshi Shimosato, Tomohide Takaya
Summary: The study explores the use of novel myoDNs to promote the differentiation of myoblasts in diabetic patients, demonstrating that this approach effectively improves muscle wasting and has similar effects on healthy myoblasts exposed to high glucose or high lipid levels.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Immunology
Takuma Okajima, Suguru Shigemori, Fu Namai, Tasuku Ogita, Takashi Sato, Takeshi Shimosato
Summary: The study found that prophylactic feeding of ODNcap can reduce allergic airway inflammation, hyperresponsiveness, and goblet cell hyperplasia in an ovalbumin-induced asthma model. Transcriptomics-driven approaches demonstrated that ODNcap feeding inhibits injury of pulmonary vein cardiomyocytes. Additionally, airway antimicrobial peptide and fecal microbiota participate in the ODNcap-mediated effects.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Kanako Shinada, Takashi Sato, Saya Moriyama, Yu Adachi, Masahiro Shinoda, Shinichiro Ota, Miwa Morikawa, Masamichi Mineshita, Takayuki Matsumura, Yoshimasa Takahashi, Masaharu Shinkai
Summary: The use of favipiravir in the treatment of COVID-19 accelerates viral clearance, preserves the generation and persistence of neutralizing antibodies, and does not interfere with the maturation of neutralizing potency against both WT SARS-CoV-2 and its variants.
Article
Chemistry, Multidisciplinary
Yuma Nihashi, Mana Miyoshi, Koji Umezawa, Takeshi Shimosato, Tomohide Takaya
Summary: The study identified an 18-base oligodeoxynucleotide (ODN) called iSN40 that enhances the activity and gene expression of osteoblasts, promoting their differentiation and calcium deposition. iSN40 may serve as a potential nucleic acid drug for osteoporosis therapy.
Article
Biochemistry & Molecular Biology
Naoki Nohira, Sayaka Shinji, Shunichi Nakamura, Yuma Nihashi, Takeshi Shimosato, Tomohide Takaya
Summary: In this study, it was found that embryonal rhabdomyosarcoma (ERMS) cells express nucleolin and their growth can be inhibited by anti-nucleolin aptamers. Further investigation revealed that iSN04 and AS1411, the two anti-nucleolin aptamers, not only suppress the proliferation of ERMS cells, but also promote their differentiation and myogenesis. Additionally, these anti-nucleolin aptamers disrupt the formation of tumor spheres in three-dimensional culture. Therefore, anti-nucleolin aptamers have the potential to be used as nucleic acid drugs for chemotherapy against ERMS.
Article
Biotechnology & Applied Microbiology
Fu Namai, Shunsuke Sumiya, Natsumi Nomura, Takashi Sato, Takeshi Shimosato
Summary: We developed genetically modified lactic acid bacteria (gmLAB) that produce green fluorescent protein (GFP)-conjugating, anti-programmed death-ligand 1 (PD-L1) single-chain variable fragments (scFv) for targeted cancer therapy. The gmLAB was able to express the anti-PD-L1 scFv and show specificity towards PD-L1 protein. These findings suggest that gmLAB could serve as an affordable diagnostic and treatment tool for cancer research.
Article
Biochemistry & Molecular Biology
Suzuka Oshima, Fu Namai, Takashi Sato, Takeshi Shimosato
Summary: In this study, a scFv based on the amino acid sequence of CC12.1 was designed to bind to the RBD of SARS-CoV-2 and enter cells through ACE2 receptors. Compared with the original antibody, scFv has the advantages of high tissue penetration and low production cost. gmLAB were constructed to incorporate the designed scFv and could be used as microbial therapeutics against COVID-19.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Tomohiro Takano, Takashi Sato, Ryutaro Kotaki, Saya Moriyama, Shuetsu Fukushi, Masahiro Shinoda, Kiyomi Kabasawa, Nagashige Shimada, Mio Kousaka, Yu Adachi, Taishi Onodera, Kazutaka Terahara, Masanori Isogawa, Takayuki Matsumura, Masaharu Shinkai, Yoshimasa Takahashi
Summary: Takano et al. demonstrate that a heterologous booster using a SARS-CoV-2 recombinant spike protein vaccine induces a more sustained and broader anti-spike receptor-binding domain antibody response compared to a homologous booster using an mRNA vaccine. The study shows that the S-268019-b spike protein booster generates higher and longer-lasting IgG titers specific to the SARS-CoV-2 spike receptor-binding domain, with the ability to bind to antigenically distinct variants, while the BNT162b2 mRNA homologous booster has a weaker effect. Additionally, the S-268019-b booster enhances the production of RBD-angiotensin-converting enzyme 2 (ACE2) binding inhibitory antibodies, resulting in increased neutralizing activities against Omicron BA.1 and BA.5 variants.
NATURE COMMUNICATIONS
(2023)
Article
Medicine, General & Internal
Masahiro Shinoda, Shinichiro Ota, Yuto Yoshida, Takatomo Hirouchi, Kanako Shinada, Takashi Sato, Miwa Morikawa, Naoki Ishii, Masaharu Shinkai
Summary: High fever, widespread viral pneumonia, and pleural effusion may serve as easily measurable prognostic indicators in COVID-19 patients with similar backgrounds.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Merisa Sirisopapong, Takeshi Shimosato, Supattra Okrathok, Sutisa Khempaka
Summary: This study aimed to characterize LAB strains from the chicken digestive tract and determine their potential as probiotics. The researchers found that the L. ingluviei and L. salivarius strains exhibited probiotic properties and improved the growth of broiler chickens. Future studies should investigate their potential for growth improvement in broilers.
Article
Biochemistry & Molecular Biology
Mina Ishioka, Yuma Nihashi, Yoichi Sunagawa, Koji Umezawa, Takeshi Shimosato, Hiroshi Kagami, Tatsuya Morimoto, Tomohide Takaya
Summary: This study found that inhibition of nucleolin by iSN04 can promote the differentiation of cardiac mesoderm into cardiomyocytes but interferes with the differentiation of early mesoderm into the cardiac lineage. This is the first report on generating cardiomyocytes from pluripotent stem cells using a DNA aptamer. iSN04 does not induce hypertrophic responses in primary-cultured cardiomyocytes, making it useful and safe for regenerative therapy of heart failure using stem cell-derived cardiomyocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Yuma Nihashi, Sayaka Shinji, Koji Umezawa, Takeshi Shimosato, Tamao Ono, Hiroshi Kagami, Tomohide Takaya
Summary: Research has shown that certain myogenetic oligodeoxynucleotides (myoDNs) can promote the differentiation of chicken myoblasts into MHC-positive myotubes.
ANIMAL SCIENCE JOURNAL
(2021)
