Article
Agronomy
Marzieh Attarianfar, Azam Mikani, Mohammad Mehrabadi
Summary: Insect growth regulators (IGR) are a good option for insect pest management due to their minimal adverse effects on humans and animals. This study investigated the effects of the JH analog fenoxycarb on the antiviral immunity pathway and susceptibility to Helicoverpa armigera nuclear polyhedrosis virus (HaNPV) in H. armigera larvae. The results showed that fenoxycarb decreased the expression of core genes involved in miRNA, siRNA, and apoptosis, and increased mortality and replication of HaNPV in the larvae.
PEST MANAGEMENT SCIENCE
(2023)
Article
Infectious Diseases
Yung-Che Chen, Chang -Chun Hsiao, Chao-Chien Wu, Tung-Ying Chao, Sum-Yee Leung, Yu -Ping Chang, Chia-Cheng Tseng, Chiu-Ping Lee, Po-Yuan Hsu, Ting-Ya Wang, Po-Wen Wang, Ting-Wen Chen, Meng-Chih Lin
Summary: This study explores a new approach for treating active tuberculosis (TB) by screening and validating microRNAs with immune-regulatory functions. The research identified differentially expressed microRNAs associated with TB and revealed their roles in regulating autophagy, apoptosis, and macrophage phagocytosis. The findings provide evidence for microRNA-based host-directed immunotherapy and offer new therapeutic targets for treatment-refractory TB.
JOURNAL OF INFECTION
(2022)
Article
Cell Biology
Lu Li, Peike Sheng, Tianqi Li, Christopher J. Fields, Nicholas M. Hiers, Yuzhi Wang, Jianping Li, Casey M. Guardia, Jonathan D. Licht, Mingyi Xie
Summary: Li et al. systematically analyzed Argonaute-CLASH data to identify candidate target RNA-directed miRNA degradation (TDMD) triggers that induce nontemplated nucleotide addition at the miRNA 3' end. They found widespread TDMD triggers in target RNAs that can cooperate with encoded proteins to induce miRNA degradation.
GENES & DEVELOPMENT
(2021)
Review
Cell Biology
Shintaro Komatsu, Hiroki Kitai, Hiroshi I. Suzuki
Summary: MicroRNAs (miRNAs) act as versatile regulators of gene expression at the post-transcriptional level. Recent studies have uncovered the mechanisms involved in miRNA biogenesis, including the processing of both canonical and non-canonical miRNAs. Additionally, the complex interactions between miRNAs and their targets have been explored, revealing target-mediated miRNA degradation and cooperative regulation by multiple miRNAs. These findings highlight the coordinated regulation of miRNA biogenesis and miRNA-target interaction in miRNA biology.
Article
Immunology
Abdollah Jafarzadeh, Alma Naseri, Layla Shojaie, Maryam Nemati, Sara Jafarzadeh, Hossein Bannazadeh Baghi, Michael R. Hamblin, Seyed Amirreza Akhlagh, Hamed Mirzaei
Summary: MiR-155 plays a crucial role in regulating immune responses during viral infections, affecting both innate and adaptive immunity. Proper regulation of miR-155 expression is essential for an effective anti-virus immune response and viral clearance.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Weihong Xie, Shouheng Jin, Chenqiu Zhang, Shuai Yang, Yaoxing Wu, Yong Zhao, Zhou Songyang, Jun Cui
Summary: Our study identifies E3 ubiquitin ligase NEDD4 as a negative regulator of type I interferon signaling by targeting TBK1 for degradation at the late stage of viral infection. Mechanistically, NEDD4 catalyzes the K27-linked poly-ubiquitination of TBK1 at K344, providing a recognition signal for NDP52-mediated selective autophagic degradation. This reveals the regulatory role of NEDD4 in balancing TBK1-centered type I IFN activation and provides insights into the crosstalk between selective autophagy and antiviral signaling.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Immunology
Hongliang Zuo, Xinxin Liu, Mengting Luo, Linwei Yang, Zhiming Zhu, Shaoping Weng, Jianguo He, Xiaopeng Xu
Summary: A novel miRNA named miR-10c was identified in Litopenaeus vannamei, playing a crucial regulatory role in shrimp immune responses by targeting LvToll3 to inhibit its expression and influencing the activation of IRF, thereby facilitating white spot syndrome virus infection. The study provides insights into the participation of miRNA in immune response regulation and enhances understanding of Toll-induced immune responses in L. vannamei.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Junxia Cui, Weiwei Zheng, Tianjun Xu, Yuena Sun
Summary: Long noncoding RNAs (lncRNAs) play an important role in the immune response of fish to pathogen infections. This study found that MIR122HG can negatively regulate the antiviral immune response in miiuy croaker and prevent excessive immune response. Furthermore, MIR122HG promotes viral replication.
Article
Biochemistry & Molecular Biology
Fabian Kern, Ernesto Aparicio-Puerta, Yongping Li, Tobias Fehlmann, Tim Kehl, Viktoria Wagner, Kamalika Ray, Nicole Ludwig, Hans-Peter Lenhof, Eckart Meese, Andreas Keller
Summary: Researching which genes, gene sets or pathways are regulated by specific miRNAs can be time consuming and labor intensive, especially for researchers without computational experience. MiRTargetLink 2.0 offers a comprehensive solution for analyzing and visualizing miRNA networks in human, mouse and rat.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Xinglu Chen, Longmei Qiu, Xirui Si, Xiaolin Zhang, Baoying Guo, Zhi Liao, Xiaojun Yan, Pengzhi Qi
Summary: This study investigated the association between Mc-novel_miR_145 and IL-17 homolog (McIL-17-3) and their immunomodulatory effects in Mytilus coruscus. The results showed that McIL-17-3 was highly expressed in immune-associated tissues and responded to bacterial challenges. McIL-17-3 could activate downstream NF-kappa b and was negatively regulated by Mc-novel_miR_145. Moreover, Mc-novel_miR_145 could alleviate LPS-induced apoptosis by negatively regulating McIL-17-3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Antonella Antignani, Maria Teresa Bilotta, Jacob S. Roth, Daniel J. Urban, Min Shen, Matthew D. Hall, David FitzGerald
Summary: A study found that IAPs are a barrier to the efficacy of immunotoxin treatment, which can be overcome with combination treatments that include Smac mimetics.
Article
Fisheries
Lise Chaumont, Bertrand Collet, Pierre Boudinot
Summary: The interferon-inducible double-stranded RNA-dependent protein kinase (PKR) is a key antiviral mechanism of the innate immune system. It gets activated upon binding viral double stranded RNA and phosphorylates eukaryotic translation initiation factor 2 alpha (eIF2 alpha), resulting in protein shut-down that limits viral replication. Fish Pkr and fish-specific paralogue Z-DNA-dependent protein kinase (Pkz) also play important roles in antiviral defense.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Enzo Z. Poirier, Michael D. Buck, Probir Chakravarty, Joana Carvalho, Bruno Frederico, Ana Cardoso, Lyn Healy, Rachel Ulferts, Rupert Beale, Caetano Reis e Sousa
Summary: The discovery of a Dicer isoform called antiviral Dicer provides protection for tissue stem cells against RNA viruses, shedding light on the molecular regulation of antiviral RNAi in mammalian innate immunity. Different cell-intrinsic antiviral pathways can be tailored to the differentiation status of cells.
Article
Cell Biology
Xibao Zhao, Qianqian Di, Juan Yu, Jiazheng Quan, Yue Xiao, Huihui Zhu, Hongrui Li, Jing Ling, Weilin Chen
Summary: Research has shown that USP19 plays a crucial role in promoting TBK1 lysosomal degradation through chaperone-mediated autophagy, contributing to immune response and infectious diseases. This study uncovers a novel role for USP19 in CMA-mediated TBK1 degradation.
Review
Biochemistry & Molecular Biology
Szilvia Lukacsi, Zsolt Farkas, Eva Saskoi, Zsuzsa Bajtay, Krisztina Takacs-Vellai
Summary: Endocytosis provides cellular nutrition and homeostasis, while pathogens often exploit this entry point to infect host cells. Phagocytosis plays a key role in protecting against invading microbes, with initial recognition of infection differing between mammals and C. elegans. However, molecules involved in efferocytosis are highly conserved between the two species, making C. elegans a powerful model for studying phagocytic mechanisms. Additionally, studies in both mammals and worms show that defective apoptotic cell clearance can lead to the activation of innate immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Geng Yang, Jiajia Wang, Tao Luo, Xiaobo Zhang
Article
Biochemistry & Molecular Biology
Siyuan Zhang, Xiaobo Zhang
Article
Biochemistry & Molecular Biology
Yuechao Sun, Yu Zhang, Xiaobo Zhang
Article
Cell & Tissue Engineering
Fan Yang, Pei Cui, Yu Lu, Xiaobo Zhang
STEM CELL RESEARCH & THERAPY
(2019)
Article
Fisheries
Wenlin Wu, Congjie Dai, Xunwei Duan, Cuifang Wang, Xiaosi Lin, Jiaying Ke, Yixuan Wang, Xiaobo Zhang, Haipeng Liu
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Oncology
Yi Gong, Geng Yang, Qizhi Wang, Yumeng Wang, Xiaobo Zhang
MOLECULAR CANCER RESEARCH
(2020)
Article
Medicine, Research & Experimental
Song Zhang, Cuilian Liu, Xiaobo Zhang
MOLECULAR THERAPY-NUCLEIC ACIDS
(2019)
Article
Cell & Tissue Engineering
Wenlin Wu, Chenxi Xu, Xiaobo Zhang, An Yu, Le Shu
STEM CELL RESEARCH & THERAPY
(2020)
Article
Microbiology
Xumei Sun, Junyi Yang, Minhui Zheng, Xiaobo Zhang
Summary: The study developed a biocoenosis generator that continuously released methane on the 1000-m deep-sea floor of the South China Sea, artificially constructing a deep-sea ecosystem biocoenosis. It was found that deep-sea biocoenosis consisted of anaerobic methane-oxidizing archaea, symbiotic archaea, bacteria, and animals, with viruses coexisting during the origin of the ecosystem.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Medicine, Research & Experimental
Yalei Cui, Haitao Wan, Xiaobo Zhang
Summary: The study identified shrimp miR-34 as a potential antiviral miRNA against WSSV infection and suppressor of cancer progression, suggesting that animal antiviral miRNAs may have anti-tumor activity in humans.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Cell Biology
Chenxi Xu, Jianjian Zhuang, Xiaobo Zhang
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Siyuan Zhang, Fan Xin, Xiaobo Zhang
Summary: Compounds packaged in virions of white spot syndrome virus can trigger the viral life cycle by enhancing host glycolysis, leading to virus infection. This process involves the binding of palmitic amide to triosephosphate isomerase and the upregulation of HIF-1, enhancing glycolysis and promoting virus infection.
Review
Fisheries
Yi Gong, Xiaobo Zhang
Summary: Shrimp have developed innate immunity to fight against more than 20 viruses, with RNA interference (RNAi) playing a key role. Short interfering RNAs (siRNAs) and microRNAs (miRNAs) are important in virus-host interactions. The non-seed sequence in miRNAs is essential for their targeting function in regulating virus-host interactions.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Mengqi Chu, Haitao Wan, Xiaobo Zhang
Summary: The study revealed that hnRNP A2B1 is significantly upregulated in melanoma stem cells, playing an important role in promoting the expression of anti-apoptosis genes and suppressing pro-apoptosis genes by regulating their splicing. This ultimately leads to cell cycle arrest in G2 phase and apoptosis of melanoma stem cells, highlighting the crucial requirement of hnRNP A2B1 in tumorigenesis and providing novel insights into the role of hnRNPs in cancer stem cells.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Chemistry, Analytical
Jianjian Zhuang, Haitao Wan, Xiaobo Zhang
Summary: A biosensor using AuNP-modified AuE and CPs was developed for the detection of miR-100 in GC patients' sera. The biosensor showed high specificity and sensitivity in distinguishing miR-100 variants, with a detection range from 100 aM to 10 pM and a LOD of 100 aM. The cutoff for gastric cancer detection was determined to be 5 fM based on testing 100 human sera samples.