Article
Immunology
Kayleigh Walker, Roberta Baravalle, Rachel Holyfield, Jacqueline Kalms, Helena Wright, Chitra Seewooruthun, Frederick W. W. Muskett, Anthony Scott-Tucker, Andy Merritt, Alistair Henry, Alastair D. G. Lawson, Gareth Hall, Christine Prosser, Mark D. D. Carr
Summary: In this study, a diverse panel of single-domain antibodies (VHHs) that bind to IL-13 and inhibit downstream IL-13 signaling were identified. The study also revealed a novel allosteric mechanism of inhibition and potential small-molecule drug targets for IL-13.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Irina A. Favorskaya, Dmitry V. Shcheblyakov, Ilias B. Esmagambetov, Inna V. Dolzhikova, Irina A. Alekseeva, Anastasia I. Korobkova, Daria V. Voronina, Ekaterina I. Ryabova, Artem A. Derkaev, Anna V. Kovyrshina, Anna A. Iliukhina, Andrey G. Botikov, Olga L. Voronina, Daria A. Egorova, Olga V. Zubkova, Natalia N. Ryzhova, Ekaterina I. Aksenova, Marina S. Kunda, Denis Y. Logunov, Boris S. Naroditsky, Alexandr L. Gintsburg
Summary: Virus-neutralizing antibodies are crucial in treating COVID-19, but the emergence of SARS-CoV-2 variants with reduced sensitivity to existing antibody therapies necessitates the development of more potent and broad-spectrum neutralizing antibodies. This study isolated a panel of single-domain antibodies that specifically bind to the receptor-binding domain of the SARS-CoV-2 S glycoprotein. By generating dimeric molecules from the selected antibodies, the researchers achieved a significant increase in neutralizing activity, with the most potent heterodimeric molecule effectively neutralizing all major SARS-CoV-2 variants of concern. These findings highlight the potential of this heterodimeric molecule as a promising candidate for COVID-19 treatment.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Mehdi Arbabi-Ghahroudi
Summary: Since the discovery of camelid heavy-chain antibodies in 1993, there has been great interest in the research and application of nanobodies. After years of effort, the first nanobody drug was approved in 2018-2019 for the treatment of rare blood clotting disorders. Despite intellectual property restrictions, nanobodies have the potential to become mainstream biotherapeutics in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Julia Hambach, Anna Marei Mann, Peter Bannas, Friedrich Koch-Nolte
Summary: This article discusses the specificity and effectiveness of nanobody-based constructs in targeting CD38-expressing myeloma cells. Promising results have been obtained in preclinical studies, warranting further clinical research to evaluate the potential of these CD38-specific nanobody-based constructs for the treatment of multiple myeloma.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Liping Liao, Wenzhen Dang, Tingting Lin, Jinghua Yu, Tonghai Liu, Wen Li, Senhao Xiao, Lei Feng, Jing Huang, Rong Fu, Jiacheng Li, Liping Liu, Mingchen Wang, Hongru Tao, Hualiang Jiang, Kaixian Chen, Xingxing Diao, Bing Zhou, Xiaoyan Shen, Cheng Luo
Summary: PGK1 plays a role in immunometabolism by influencing metabolic status. The inhibitor DC-PGKI can suppress the activity of PGK1 and alleviate symptoms of inflammatory bowel disease.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Cell & Tissue Engineering
Chenyang Liu, Yan Xu, Yichi Lu, Pan Du, Xiaoxiao Li, Chengchun Wang, Peng Guo, Ling Diao, Guozhong Lu
Summary: The study demonstrates that S-IT MSCs can effectively induce M2 polarization of macrophages via IL-6 dependent signaling pathways and accelerate wound healing. Results show that S-IT MSCs significantly enhance the ability of M2 phenotype to promote vascularization and collagen deposition, ultimately improving wound healing quality.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Oncology
Elisa C. Toffoli, Abdolkarim Sheikhi, Roeland Lameris, Lisa A. King, Amanda van Vliet, Bruce Walcheck, Henk M. W. Verheul, Jan Spanholtz, Jurriaan Tuynman, Tanja D. de Gruijl, Hans J. van der Vliet
Summary: A novel bispecific single domain antibody (VHH) targeting CD16 on NK cells and EGFR on tumor cells enhanced NK cell activation and cytotoxicity, showing potential for cancer therapy. This approach demonstrated efficacy in controlling tumor growth in colorectal cancer cell co-cultures, suggesting promise for further exploration in NK cell-based therapeutic approaches.
Review
Immunology
Kristian Daniel Ralph Roth, Esther Veronika Wenzel, Maximilian Ruschig, Stephan Steinke, Nora Langreder, Philip Alexander Heine, Kai-Thomas Schneider, Rico Ballmann, Viola Fuehner, Philipp Kuhn, Thomas Schirrmann, Andre Frenzel, Stefan Duebel, Maren Schubert, Gustavo Marcal Schmidt Garcia Moreira, Federico Bertoglio, Giulio Russo, Michael Hust
Summary: Antibodies are crucial for the diagnosis and treatment of diseases caused by pathogens. Their importance has been highlighted during the COVID-19 pandemic, and new technologies like antibody phage display have advanced the discovery of human monoclonal antibodies for therapeutic purposes. These technologies have expanded the possibilities for generating antibodies against a wide range of pathogens and toxins.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Oncology
Yasaman Asaadi, Fatemeh Fazlollahi Jouneghani, Sara Janani, Fatemeh Rahbarizadeh
Summary: By comparing the structure and properties of camelid antibodies (VHHs) and single-chain variable fragments (scFvs), it can be determined which one is more suitable for their respective therapeutic, diagnostic, and research applications.
BIOMARKER RESEARCH
(2021)
Article
Plant Sciences
Adel M. Zakri, Abdullah A. Al-Doss, Ahmed A. Ali, Emad M. Samara, Basem S. Ahmed, Mohammed A. Al-Saleh, Ali M. Idris, Omar A. Abdalla, Markus Sack
Summary: This study isolated two camel-derived nanobodies against Tomato leaf curl Sudan virus (ToLCSDV) using phage display technology. The nanobodies also cross-reacted with Tomato yellow leaf curl virus and showed significant enzyme-linked immunosorbent assay reactivity with plants infected with typical begomovirus symptoms. These results pave the way for developing diagnostics for begomovirus detection, design of novel nanomaterials based on virus-like particles, and nanobody-mediated begomovirus resistance in economically important crops.
Article
Biochemistry & Molecular Biology
Charles A. Swofford, Sarah A. Nordeen, Lu Chen, Mahaam M. Desai, Joanna Chen, Stacy L. Springs, Thomas U. Schwartz, Anthony J. Sinskey
Summary: Misuse of antibiotics in aquaculture poses a public health risk and leads to the emergence of multiresistant pathogens. Rapid test kits are used to monitor food supply chains but lack accuracy. Researchers have developed single-domain antibodies that can improve the reliability and shelf-life of these test kits for small molecule antibiotics.
Article
Biochemistry & Molecular Biology
Charles A. Swofford, Sarah A. Nordeen, Lu Chen, Mahaam M. Desai, Joanna Chen, Stacy L. Springs, Thomas U. Schwartz, Anthony J. Sinskey
Summary: Antibiotics in aquaculture are important for preventing bacterial infections in fish, but their misuse poses a public health risk and contributes to the unintentional creation of multiresistant pathogens. Current rapid test kits for these drugs lack high accuracy, but discovering and engineering single-domain antibodies (VHHs) can improve the reliability and shelf-life of these test kits. By developing a novel anti-chloramphenicol VHH with improved binding affinity, the structure of VHH-hapten interactions provides insights for future engineering of VHHs against additional targets.
Article
Oncology
Saisai Chen, Kai Lu, Yue Hou, Zonghao You, Chuanjun Shu, Xiaoying Wei, Tiange Wu, Naipeng Shi, Guangyuan Zhang, Jianping Wu, Shuqiu Chen, Lihua Zhang, Wenchao Li, Dingxiao Zhang, Shenghong Ju, Ming Chen, Bin Xu
Summary: The study found that high expression of YY1 is closely associated with M2 macrophages in prostate cancer, promoting tumor development. The study also revealed that treatment targeting YY1 can suppress tumor metastasis and generate synergistic anti-tumor effects. Furthermore, the study revealed that YY1 upregulates IL-6 expression by regulating enhancer-promoter interactions, thereby promoting tumor progression.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Review
Biochemistry & Molecular Biology
Huaping Tang, Yuan Gao, Jiangyuan Han
Summary: The camelid-derived single chain antibody (sdAb), or nanobody, is a unique functional heavy-chain antibody that has high solubility and similar antigen-binding affinity to conventional antibodies, making it a promising tool in biomedicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Sunkyung Kim, Jing Chen, Suin Jo, Feiya Ou, Stephen T. Ferris, Tian-Tian Liu, Ray A. Ohara, I. I. I. David A. Anderson, Renee Wu, Michael Y. Chen, William E. Gillanders, William E. Gillanders, Theresa L. Murphy, Kenneth M. Murphy
Summary: We demonstrate that tumor-derived IL-6 inhibits the development of type 1 classical dendritic cells (cDC1) by inducing C/EBPβ expression in common dendritic cell progenitors (CDPs). This leads to the upregulation of Zeb2 expression in the -165 kb enhancer, preventing the specification of pre-cDC1. Our findings explain how tumor-associated IL-6 suppresses cDC1 development and suggest that targeting abnormal C/EBPβ induction in CDPs could enhance antitumor immunity.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Gastroenterology & Hepatology
Bosse Jessen, Yasmina Rodriguez-Sillke, Elena Sonnenberg, Michael Schumann, Andrey Kruglov, Inka Freise, Franziska Schmidt, Jochen Maul, Anja A. Kuehl, Rainer Glauben, Donata Lissner, Britta Siegmund
Summary: The study suggests that high levels of tumor necrosis factor produced by peripheral blood mononuclear cells in patients with inflammatory bowel disease can predict their response to infliximab therapy. Measuring TNF concentration before treatment can help identify patients who are most likely to benefit from the therapy.
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Biographical-Item
Oncology
Robert Peter Gale, Grigory A. Efimov
Article
Allergy
Ines Zettl, Tatiana Ivanova, Maria R. Strobl, Christina Weichwald, Oksana Goryainova, Evgenia Khan, Marina Rutovskaya, Margarete Focke-Tejkl, Anja Drescher, Barbara Bohle, Sabine Flicker, Sergei Tillib
Summary: The study identified high-affinity Bet v 1-specific nanobodies that can recognize an important IgE epitope and reduce allergen-induced basophil activation, indicating that allergen-specific nanobodies are useful tools for future treatment of pollen allergy.
Article
Gastroenterology & Hepatology
Munevver Demir, Sonja Lang, Phillipp Hartmann, Yi Duan, Anna Martin, Yukiko Miyamoto, Marina Bondareva, Xinlian Zhang, Yanhan Wang, Philipp Kasper, Corinna Bang, Christoph Roderburg, Frank Tacke, Hans-Michael Steffen, Tobias Goeser, Andrey Kruglov, Lars Eckmann, Peter Starkel, Derrick E. Fouts, Bernd Schnabl
Summary: The study found that the fecal fungal composition of patients with non-alcoholic fatty liver disease (NAFLD) is associated with disease severity. Non-obese NAFLD patients have a distinct fungal ratio in their feces compared to other types of patients. Antifungal treatment in a mouse model can reduce liver damage from steatohepatitis.
JOURNAL OF HEPATOLOGY
(2022)
Article
Immunology
Justus Ninnemann, Caroline Winsauer, Marina Bondareva, Anja A. Kuehl, Laura Lozza, Pawel Durek, Donata Lissner, Britta Siegmund, Stefan H. E. Kaufmann, Mir-Farzin Mashreghi, Sergei A. Nedospasov, Andrey A. Kruglov
Summary: Successful treatment of chronic inflammatory diseases requires both the cessation of inflammation and the promotion of tissue repair. Targeting tumor necrosis factor (TNF) can induce tissue repair in inflammatory bowel disease (IBD) patients, but the underlying molecular mechanisms have been unclear. Using an experimental colitis model, this study found that TNF interferes with tissue repair by inducing a soluble antagonist of IL-22. Furthermore, membrane-bound TNF expressed by T cells perpetuates colonic inflammation, while soluble TNF produced by epithelial cells inhibits colonic epithelial repair.
MUCOSAL IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lilia I. Mukhametova, Sergei A. Eremin, Dmitrii A. Arutyunyan, Oksana S. Goryainova, Tatiana I. Ivanova, Sergei V. Tillib
Summary: Due to its unique structure and properties, human breast milk lactoferrin (hLF) has many nutritional and health-promoting functions in infants. Noncompetitive polarization fluorescence immunoassay (FPIA) is a promising method for hLF quantification in milk and dairy products, which does not require the separation of the bound and free protein and allows to avoid time-consuming sample preparation.
BIOCHEMISTRY-MOSCOW
(2022)
Article
Biochemistry & Molecular Biology
Taisiya R. Yurakova, Ekaterina O. Gubernatorova, Ekaterina A. Gorshkova, Maxim A. Nosenko, Sergei A. Nedospasov, Marina S. Drutskaya
Summary: This study explores the immune-metabolic reprogramming of myeloid cells in the development of asthma and identifies the association between a specific immunometabolic phenotype, mitochondrial morphology, ROS production in macrophages, and the TLR4-dependent processes. These findings provide insight into the molecular mechanisms underlying asthma and may offer potential therapeutic strategies for its treatment.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Immunology
M. Bondareva, P. Letz, K. Karberg, E. Schrezenmeier, I Semin, H. Rincon-Arevalo, T. Doerner, M. F. Mashreghi, A-L Stefanski, A. A. Kruglov
Summary: Systemic vaccination against SARS-CoV-2 induces high levels of specific antibodies in the blood and oral cavity. However, patients with rheumatoid arthritis (RA) and undergoing B cell depletion treatment have a higher risk of severe COVID-19 manifestation and breakthrough infections. This study found that the levels of secreted antibodies in the oral cavity were correlated with B cell numbers in the blood. Importantly, a third dose vaccination efficiently induced anti-Spike antibody responses in the saliva of RA patients, similar to healthy individuals.
JOURNAL OF AUTOIMMUNITY
(2022)
Article
Immunology
Ines Zettl, Tatiana Ivanova, Mohammed Zghaebi, Marina Rutovskaya, Isabella Ellinger, Oksana Goryainova, Jessica Kollarova, Sergio Villazala-Merino, Christian Lupinek, Christina Weichwald, Anja Drescher, Julia Eckl-Dorna, Sergei Tillib, Sabine Flicker
Summary: This study reported the generation and characterization of ICAM-1-specific nanobodies, which showed high affinity binding to ICAM-1 and were not internalized by cells. The findings suggest that these nanobodies can be used in combination with allergen-specific nanobodies to develop topical treatments for pollen allergy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, General & Internal
Tatiana I. Ivanova, Ilya D. Klabukov, Ludmila I. Krikunova, Marina V. Poluektova, Natalia I. Sychenkova, Vera A. Khorokhorina, Nikolay V. Vorobyev, Margarita Ya. Gaas, Denis S. Baranovskii, Oksana S. Goryainova, Anastasiya M. Sachko, Peter V. Shegay, Andrey D. Kaprin, Sergei V. Tillib
Summary: This study evaluated the role of transferrin (Tf) in ovarian cancer (CrO) and related functional iron deficiency (FID), and found that Tf may be a promising tool for diagnosing both CrO and associated FID.
JOURNAL OF CLINICAL MEDICINE
(2022)
Meeting Abstract
Immunology
I. Zettl, T. Ivanova, M. Zghaebi, M. V. Rutovskaya, I. Ellinger, O. Goryainova, J. Kollarova, S. Villazala-Merino, C. Lupinek, C. Weichwald, A. Drescher, J. Eckl-Dorna, S. V. Tillib, S. Flicker
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Meeting Abstract
Immunology
C. Bauernfeind, I. Zettl, T. Ivanova, O. Goryainova, A. Drescher, S. V. Tillib, S. Flicker
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
S. Tillib, O. S. Goryainova, A. M. Sachko, T. I. Ivanova
Summary: This article presents the analysis of two variants of nanobodies obtained using a highly efficient technology, which can recognize human transferrin. It demonstrates the unique potential of these nanobodies for diagnostic studies.
Article
Biochemistry & Molecular Biology
S. Tillib, O. S. Goryainova, A. M. Sachko, T. I. Ivanova, M. Ya Gaas, N. V. Vorob'ev, A. D. Kaprin, P. Shegay
Summary: Nanobody-based tools have shown unique potential in the noninvasive diagnosis of bladder cancer by specifically enriching and removing certain antigenic proteins.
Article
Immunology
Maria Byazrova, Gaukhar Yusubalieva, Anna Spiridonova, Grigory Efimov, Dmitriy Mazurov, Konstantin Baranov, Vladimir Baklaushev, Alexander Filatov
Summary: The study analyzed the generation of plasmablasts and RBD-specific memory B cells in patients during the acute phase of COVID-19. The activation and proliferation of Bmem cells were induced which led to the generation of plasmablast phenotypic cells and RBD-specific ASCs. Additionally, it was found that SARS-CoV-2-specific Bmem cells are generated during the acute phase of COVID-19, providing a basis for further studies on the longevity of SARS-CoV-2-specific B-cell memory.
CLINICAL & TRANSLATIONAL IMMUNOLOGY
(2021)