Review
Biochemistry & Molecular Biology
Masayuki Yamashita, Atsushi Iwama
Summary: This review summarizes the changes in hematopoietic stem cells (HSCs) during aging and how different clones of HSCs can be expanded. Research shows that in mice and humans, the clonal behavior of HSCs is closely related to the risk of hematological malignancies and cardiovascular diseases. Therefore, understanding the impact of aging on the hematopoietic system is critical to prevent or overcome age-related changes in the blood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Michael D. Kessler, Amy Damask, Sean O'Keeffe, Nilanjana Banerjee, Dadong Li, Kyoko Watanabe, Anthony Marketta, Michael Van Meter, Stefan Semrau, Julie Horowitz, Jing Tang, Jack A. Kosmicki, Veera M. Rajagopal, Yuxin Zou, Yariv Houvras, Arkopravo Ghosh, Christopher Gillies, Joelle Mbatchou, Ryan R. White, Niek Verweij, Jonas Bovijn, Neelroop N. Parikshak, Michelle G. LeBlanc, Marcus Jones, David J. Glass, Luca A. Lotta, Michael N. Cantor, Gurinder S. Atwal, Adam E. Locke, Manuel A. R. Ferreira, Raquel Deering, Charles Paulding, Alan R. Shuldiner, Gavin Thurston, Adolfo A. Ferrando, Will Salerno, Jeffrey G. Reid, John D. Overton, Jonathan Marchini, Hyun M. Kang, Aris Baras, Goncalo R. Abecasis, Eric Jorgenson
Summary: Clonal haematopoiesis, associated with ageing and adverse health outcomes, involves the expansion of certain blood cell lineages. This study identified novel germline genetic variations influencing predisposition to clonal haematopoiesis using exome sequence data from over 600,000 individuals. The study also found associations between clonal haematopoiesis and severe COVID-19 outcomes, cardiovascular disease, and malignancy.
Article
Multidisciplinary Sciences
Michele C. Buck, Lisa Bast, Judith S. Hecker, Jennifer Riviere, Maja Rothenberg-Thurley, Luisa Vogel, Dantong Wang, Immanuel Andrae, Fabian J. Theis, Florian Bassermann, Klaus H. Metzeler, Robert A. J. Oostendorp, Carsten Marr, Katharina S. Goetze
Summary: Clonal hematopoiesis of indeterminate potential (CHIP) is the acquisition of somatic mutations in hematopoietic stem/progenitor cells (HSPC) leading to clonal blood cell expansion. Computational modeling shows that alternative hierarchies may better describe HSPC kinetics in CHIP and MDS samples compared to the standard hematopoietic hierarchy. The reorganization of the HSPC compartment can already be detected in the premalignant CHIP state.
Article
Biology
Almut S. Eisele, Jason Cosgrove, Aurelie Magniez, Emilie Tubeuf, Sabrina Tenreira Bento, Cecile Conrad, Fanny Cayrac, Tamar Tak, Anne-Marie Lyne, Jos Urbanus, Leila Perie
Summary: This study used cellular barcoding to trace the differentiation of mouse hematopoietic stem and progenitor cells (HSPCs) after exposure to EPO. The researchers found that EPO can directly act on multipotent progenitor cells (MPP) and modulate fate by remodeling the clonal composition of the MPP pool.
Review
Cell Biology
Anna Cazzola, Giovanni Cazzaniga, Andrea Biondi, Raffaella Meneveri, Silvia Brunelli, Emanuele Azzoni
Summary: Childhood leukemia, the most common cancer in young age, may originate during in utero development. Research on the cellular origin of leukemias is crucial for understanding disease progression, refining animal models, and developing new therapeutic approaches.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Maria Xydia, Raheleh Rahbari, Eliana Ruggiero, Iain Macaulay, Maxime Tarabichi, Robert Lohmayer, Stefan Wilkening, Tillmann Michels, Daniel Brown, Sebastiaan Vanuytven, Svetlana Mastitskaya, Sean Laidlaw, Niels Grabe, Maria Pritsch, Raffaele Fronza, Klaus Hexel, Steffen Schmitt, Michael Mueller-Steinhardt, Niels Halama, Christoph Domschke, Manfred Schmidt, Christof von Kalle, Florian Schuetz, Thierry Voet, Philipp Beckhove
Summary: Regulatory T cells in breast cancer do not show clonal relationship with their circulating counterpart, but share a common origin with intratumoral antigen-experienced conventional T cells.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Jue Feng, Joseph N. Pucella, Geunhyo Jang, Marcela Alcantara-Hernandez, Samik Upadhaya, Nicholas M. Adams, Alireza Khodadadi-Jamayran, Colleen M. Lau, Marlon Stoeckius, Stephanie Hao, Peter Smibert, Aristotelis Tsirigos, Juliana Idoyaga, Boris Reizis
Summary: This study investigates the developmental origins of dendritic cells (DCs) and reveals the shared origin of pDCs and cDCs, suggesting a revised scheme of DC development.
Article
Cell Biology
Hyebeen Hong, Jongeun Lee, Guk-Yeol Park, Soeun Kim, Jiho Park, Jong Seok Park, Youngkwon Song, Sujin Lee, Tae Jin Kim, You Jeong Lee, Tae-Young Roh, Seung-Ki Kwok, Sung Won Kim, Qiumin Tan, Yoontae Lee
Summary: CIC is a key transcription factor that limits the population of B-1a cells in the adult stage and balances the formation of B-1 and B-2 cells.
Article
Oncology
Shintaro Kazama, Kazuaki Yokoyama, Toshimitsu Ueki, Hiroko Kazumoto, Hidetoshi Satomi, Masahiko Sumi, Ichiro Ito, Nozomi Yusa, Rika Kasajima, Eigo Shimizu, Rui Yamaguchi, Seiya Imoto, Satoru Miyano, Yukihisa Tanaka, Tamami Denda, Yasunori Ota, Arinobu Tojo, Hikaru Kobayashi
Summary: This case report presents a rare occurrence of concurrent LCH and AML, sharing the same driver mutations and suggesting a common clonal origin. The presence of this case supports the proposal to classify histiocytosis, including LCH, as a myeloid/myeloproliferative malignancy.
FRONTIERS IN ONCOLOGY
(2022)
Review
Immunology
Jennifer J. Trowbridge, Daniel T. Starczynowski
Summary: The aging population requires new therapeutic strategies to improve hematopoietic aging disorders; dysregulation of innate immune and inflammatory signaling leads to competitive advantage and clonal dominance; emerging concepts will reveal critical biology and therapeutic opportunities.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Biochemical Research Methods
Giulia Pais, Giulio Spinozzi, Daniela Cesana, Fabrizio Benedicenti, Alessandra Albertini, Maria Ester Bernardo, Bernhard Gentner, Eugenio Montini, Andrea Calabria
Summary: Longitudinal clonal tracking studies in gene therapy applications require comprehensive and high-throughput analysis tools. ISAnalytics is a new R package that allows users to assess safety and efficacy of treatments and analyze hematopoietic stem cell biology using vector integration sites as markers. It provides efficient data structures, reproducibility, and analytical control, making it suitable for various clonal tracking applications.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Biochemical Research Methods
Giulia Pais, Giulio Spinozzi, Daniela Cesana, Fabrizio Benedicenti, Alessandra Albertini, Maria Ester Bernardo, Bernhard Gentner, Eugenio Montini, Andrea Calabria
Summary: Comprehensive and high-throughput clonal tracking studies using vector integration sites as markers of cellular identity are supported by ISAnalytics, a new R package. ISAnalytics addresses the computational challenges of monitoring patients over a long-term follow-up and provides a wide range of functionalities for assessing safety and efficacy and supporting stem cell biology. The package integrates seamlessly with laboratory workflows and standard procedures, offering efficient data structures and interactive web reports for reproducibility and analytical control.
BRIEFINGS IN BIOINFORMATICS
(2023)
Review
Biochemistry & Molecular Biology
Shokrollah Elahi
Summary: Under physiological conditions, HSPCs in the bone marrow niches are responsible for regulating hematopoiesis, but they can be influenced by microbial agents and infection-induced mediators. This article reviews the impact of these factors on hematopoiesis and discusses how SARS-CoV-2 infection alters the hematopoietic system, including lymphopenia, thrombocytopenia, and hypercoagulability.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Allergy
Linda Quatrini, Nicola Tumino, Francesca Besi, Cecilia Ciancaglini, Federica Galaverna, Antonio Giacomo Grasso, Pietro Merli, Franco Locatelli, Paola Vacca, Lorenzo Moretta
Summary: This study investigates the effect of glucocorticoids (GCs) on the development of innate lymphoid cells (ILCs). The results demonstrate that GCs impair lymphoid development while promoting the differentiation of NK cells. Additionally, recipients of hematopoietic stem cell transplantation treated with GCs show a significant reduction in circulating hILCs.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Review
Hematology
Mariam T. Nawas, Johannes Schetelig, Frederik Damm, Ross L. Levine, Miguel-Angel Perales, Sergio A. Giralt, Marcel R. VanDenBrink, Maria E. Arcila, Ahmet Zehir, Elli Papaemmanuil, Anja Klussmeier, Alexander H. Schmidt, Stephanie Maiwald, Kelly L. Bolton, Roni Tamari
Summary: Clonal hematopoiesis refers to somatic mutations in hematopoietic stem and progenitor cells leading to clonal expansion, which increases with age; its role in hematopoietic cell transplantation is becoming increasingly important; post-transplant hematopoiesis is influenced by various factors, requiring more large-scale studies to establish a management approach for CH.
Article
Hematology
Momoko Yoshimoto
INTERNATIONAL JOURNAL OF HEMATOLOGY
(2020)
Article
Biochemical Research Methods
Hisamichi Naito, Taku Wakabayashi, Masako Ishida, Chang-Hyun Gil, Tomohiro Iba, Fitriana Nur Rahmawati, Shota Shimizu, Mervin C. Yoder, Nobuyuki Takakura
Article
Multidisciplinary Sciences
Aneel R. Bhagwani, Daniela Farkas, Brennan Harmon, Kayla J. Authelet, Carlyne D. Cool, Martin Kolb, Elena Goncharova, Mervin C. Yoder, Matthias Clauss, Robert Freishtat, Laszlo Farkas
SCIENTIFIC REPORTS
(2020)
Letter
Oncology
Quy Le, Brandon Hadland, Soheil Meshinchi, Irwin Bernstein
Editorial Material
Cardiac & Cardiovascular Systems
James Palis, Mervin C. Yoder
CIRCULATION RESEARCH
(2020)
Article
Multidisciplinary Sciences
Christian Mandrycky, Brandon Hadland, Ying Zheng
Review
Cell & Tissue Engineering
Kyle Lewis, Momoko Yoshimoto, Takanori Takebe
Summary: This review discusses the importance of the fetal liver niche in HSC expansion, which occurs during development and has great clinical potential. Emerging approaches to generate expandable HSC in cell culture are also discussed, including the use of cells or organoid models in combination with engineering and systems biology approaches. Delivery of HSC by understanding the molecular and biological interactions between HSCs and fetal liver cells for their controlled maturation and expansion will be beneficial.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Chemistry, Analytical
Zeinab Ramshani, Fei Fan, Alicia Wei, Miguel Romanello-Giroud-Joaquim, Chang-Hyun Gil, Matt George, Mervin C. Yoder, Donny Hanjaya-Putra, Satyajyoti Senapati, Hsueh-Chia Chang
Summary: The AEM-based sensing platform is a low-cost, rapid, reliable multiplexed platform that enhances detection reliability and accuracy through optimized protocols for each target, with comparable detection sensitivity and dynamic range to ELISA, and significantly reduced detection time of 1 hour.
Article
Cell Biology
Jixin Ding, Angelo A. Cardoso, Momoko Yoshimoto, Michihiro Kobayashi
Summary: The study suggests that fetal-derived T-cell precursors may serve as leukemia initiating cells, presenting a potential mechanism for leukemia development in pediatric patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Developmental Biology
Nathalia Azevedo Portilho, Rebecca Scarfo, Elisa Bertesago, Ismail Ismailoglu, Michael Kyba, Michihiro Kobayashi, Andrea Ditadi, Momoko Yoshimoto
Summary: Research has shown that Notch signaling is not essential for the formation of B1 cells, while it has a promoting effect on the formation of B2 lymphoid cells. These findings are crucial for understanding the differentiation of B-cell lineages and for their in vitro generation for therapeutic applications.
Article
Multidisciplinary Sciences
Brandon Hadland, Barbara Varnum-Finney, Stacey Dozono, Tessa Dignum, Cynthia Nourigat-McKay, Adam M. Heck, Takashi Ishida, Dana L. Jackson, Tomer Itkin, Jason M. Butler, Shahin Rafii, Cole Trapnell, Irwin D. Bernstein
Summary: In this study, the authors used single cell RNA-sequencing to map the signaling interactions regulating embryonic HSC development. They also engineered a niche that supports HSC maturation in vitro based on this knowledge.
NATURE COMMUNICATIONS
(2022)
Review
Immunology
Michihiro Kobayashi, Momoko Yoshimoto
Summary: Drs. Herzenberg and Herzenberg proposed the layered immune system hypothesis, which suggests that different types of stem cells produce specific immune cells. Recent studies have supported this hypothesis by showing the presence of fetal-derived immune cells functioning in adults. However, it is still unknown whether these fetal-derived immune cells are produced by hematopoietic stem cells during the fetal to neonatal period.
IMMUNOLOGICAL REVIEWS
(2023)
Meeting Abstract
Immunology
Michihiro Kobayashi, Astrid Kosters, Sliver Eid Bou Ghosn, Momoko Yoshimoto
JOURNAL OF IMMUNOLOGY
(2021)
Meeting Abstract
Immunology
Momoko Yoshimoto, Chika Nishida, Samuel Cornelius, Noemi Valiente, Michihiro Kobayashi
JOURNAL OF IMMUNOLOGY
(2021)
Review
Cell Biology
Adam M. Heck, Takashi Ishida, Brandon Hadland
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)