Article
Biochemistry & Molecular Biology
Katharina Woess, Yuchen Sun, Hanae Morio, Anna Stierschneider, Anna Kaufmann, Stefan Hainzl, Lisa Trattner, Thomas Kocher, Birgit Tockner, Victoria Leb-Reichl, Markus Steiner, Gabriele Brachtl, Andrew P. South, Johann W. Bauer, Julia Reichelt, Tomomi Furihata, Verena Wally, Ulrich Koller, Josefina Pinon Hofbauer, Christina Guttmann-Gruber
Summary: Conventional anti-cancer therapies lack tumor specificity and can result in iatrogenic effects. Spliceosome-mediated RNA trans-splicing (SMaRT) is a promising approach that can target tumor cells while sparing normal cells. This study demonstrates the potential of SMaRT using the RNA trans-splicing molecule RTM44 to target the cancer gene Ct-SLCO1B3 in aggressive skin cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Changdong Wang, Yanxi Shen, Yongping Ma
Summary: This study comprehensively identified and quantified protein expression profiling in gastric cancer cells treated with BF-TK/GCV for the first time. The results showed that BF-TK/GCV inhibited tumor metastasis and downregulated the expression of metastasis-related proteins, as demonstrated by immunohistochemistry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Etienne E. Muller, Dumisile Maseko, Ranmini S. Kularatne
Summary: This study conducted phenotypic and genotypic surveillance on ACV resistance in HSV-2 derived from genital ulcer samples collected in a primary healthcare facility in South Africa. The results revealed no resistance-associated mutations, but identified several known natural polymorphisms and amino acid changes of unknown significance. All cultivable HSV-2 isolates showed susceptibility to ACV.
ANTIVIRAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Katherine Kaugars, Joseph Dardick, Anna Paula de Oliveira, Kayla A. Weiss, Regy Lukose, John Kim, Lawrence Leung, Saranathan Rajagopalan, Sydney Wolin, Leor Akabas, David M. Knipe, Goran Bajic, William R. Jacobs Jr
Summary: Despite annual vaccination efforts, influenza continues to cause significant morbidity and mortality worldwide. To improve vaccine efficacy, researchers are exploring the use of antibodies that can activate immune cell effector functions, such as ADCC. Evidence suggests that ADCC is crucial for protection against influenza and other infectious diseases, leading to the development of innovative vaccine strategies such as using Delta gD-2 as a vector for a broadly protective influenza vaccine.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Jie Wang, Kun-Te Shang, Qiong-Hong Ma, Zhao-Ying Dong, Yi-Hong Chen, Yu-Feng Yao
Summary: This study aimed to determine whether HSV-1 can be transmitted through TNTs and to investigate the effect of inhibiting the Arp2/3 complex on the intercellular transmission of HSV-1. The results showed that HSV-1 can be transmitted through TNTs and that inhibiting the Arp2/3 complex reduces the number of TNTs and the spread of HSV-1. This finding provides new insights into the transmission mode of HSV-1 and suggests a potential new antiviral target.
Article
Pharmacology & Pharmacy
Yun He, Chenchen Wang, Qi Liang, Rongjie Guo, Jiaxuan Jiang, Wenhao Shen, Kai Hu
Summary: PKHB1 peptide shows potential in treating HSK by reducing HSV-1 levels, alleviating severity of HSK, and promoting recruitment and maturation of APCs.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fanghui Ren, Ryo Narita, Ahmad S. Rashidi, Stefanie Fruhwurth, Zongliang Gao, Rasmus O. Bak, Martin K. Thomsen, Georges M. G. M. Verjans, Line S. Reinert, Soren R. Paludan
Summary: Neurotropic viruses such as herpes simplex virus (HSV) can infect neurons and cause severe diseases. HSV-induced neuronal cell death is mediated by gasdermin E (GSDME) and involves endoplasmic reticulum stress, caspase activation, and mitochondria-dependent apoptosis. The necrotic neurons release alarmins, triggering inflammatory responses in microglia.
Article
Health Care Sciences & Services
Kun-Han Yang, Jen-Yang Tang, Yan-Ning Chen, Ya-Ting Chuang, I-Hsuan Tsai, Chien-Chih Chiu, Li-Jie Li, Tsu-Ming Chien, Yuan-Bin Cheng, Fang-Rong Chang, Ching-Yu Yen, Hsueh-Wei Chang
Summary: The study demonstrates that ethyl acetate Nepenthes extract (EANT) from Nepenthes thorellii x (ventricosa x maxima) selectively kills oral cancer cells through oxidative stress-mediated cell death, triggering a mixture of necrosis and apoptosis. Pretreatment with NAC, NEC1, and Z-VAD-FMK influences the death pathways induced by EANT in oral cancer cells.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Microbiology
Xiao Hu, Qiongzhen Zeng, Ji Xiao, Shurong Qin, Yuan Wang, Tianhao Shan, Di Hu, Yexuan Zhu, Kaisheng Liu, Kai Zheng, Yifei Wang, Zhe Ren
Summary: This study revealed that the activation of NLRP3 inflammasomes triggers Gasdermin D-dependent pyroptosis in mouse microglia, leading to inflammation caused by HSV-1. The expression levels of NLRP3 and IL-1 beta were significantly increased in a mouse model of herpes simplex encephalitis. These findings provide potential treatment targets for viral inflammation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Kerry J. Laing, Werner J. D. Ouwendijk, Victoria L. Campbell, Christopher L. McClurkan, Shahin Mortazavi, Michael Elder Waters, Maxwell P. Krist, Richard Tu, Nhi Nguyen, Krithi Basu, Congrong Miao, D. Scott Schmid, Christine Johnston, Georges M. G. M. Verjans, David M. Koelle
Summary: This study reveals that varicella-zoster virus-specific T cells preferentially persist as tissue-resident-memory T cells in the rash-involved skin after recovery from zoster, indicating their importance in controlling skin infections.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Narayan Bashyal, Tae-Young Lee, Da-Young Chang, Jin-Hwa Jung, Min Gyeong Kim, Rakshya Acharya, Sung-Soo Kim, Il-Hoan Oh, Haeyoung Suh-Kim
Summary: Research has shown that MSC-TK(A168H) cells modified with the HSV-TK(A168H) gene exhibit safety in pre-clinical trials, making them suitable for therapeutic use. This genetic modification makes allogeneic MSC-based ex vivo therapy safer by eliminating transplanted cells during SAEs such as uncontrolled cell proliferation.
MOLECULES AND CELLS
(2022)
Article
Oncology
Federica Foglietta, Vanessa Pinnelli, Francesca Giuntini, Nadia Barbero, Patrizia Panzanelli, Gianni Durando, Enzo Terreno, Loredana Serpe, Roberto Canaparo
Summary: The study aims to evaluate whether the new anticancer approach SDT can selectively target cancer cells while sparing normal cells. Results show that SDT has different effects on cancer cells and normal cells, indicating a potential for treatment selectivity.
Article
Chemistry, Medicinal
Houren Zhou, Hui Liu, Yue Zhang, Ying Xin, Chi Huang, Mingzhong Li, Xiaoyun Zhao, Pingtian Ding, Zhijun Liu
Summary: This study developed a nano-ultrasound contrast agent targeted at gastric tumors, which effectively improved the transfection efficiency of suicide genes in gastric cancer cells and induced significant apoptosis.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Review
Virology
Thomas Hennig, Lara Djakovic, Lars Doelken, Adam W. Whisnant
Summary: The review highlights the various impacts of HSV on RNA Polymerase II in infected cells, including changes in Pol II holoenzymes, post-translational modifications, and regulation by viral proteins. The discussion also covers specific activities such as promoter-proximal pausing, splicing, histone repositioning, and termination in relation to host genes. Technological innovations have recently reshaped understanding of previous observations and provide insights for future research directions and considerations.
Article
Pharmacology & Pharmacy
Soniya Peter, Manju Maria Mathews, Fels Saju, Swapna Paul
Summary: The purpose of this study was to optimize, formulate, and characterize Eudragit-based nanosuspension of Ganciclovir (GCV-NPs) as a carrier for ocular drug delivery. The formulated GCV-NPs showed advantages such as suitable pH, enhanced permeation, and sustained release of medication, indicating their potential role in ocular drug delivery.
JOURNAL OF PHARMACEUTICAL INNOVATION
(2023)