Letter
Gastroenterology & Hepatology
Sohyun Lee, Nanjoo Park, Sujung Yun, Eunseon Hur, Jiwon Song, Hanna Lee, Yongsug Kim, Sangryeol Ryu
Summary: The study identified the presence of plasmid-mediated quinolone resistance (PMQR) genes in Salmonella enterica isolated from human salmonellosis patients in South Korea, with fluoroquinolone resistance mainly attributed to Salmonella 4,[5],12:i:- and Salmonella Typhimurium carrying the qnr gene.
Article
Multidisciplinary Sciences
Tania S. Darphorn, Keshia Bel, Belinda B. Koenders-van Sint Anneland, Stanley Brul, Benno H. Ter Kuile
Summary: Resistance plasmids from foodborne E. coli isolates often carry resistance to multiple antimicrobials and belong to the IncI, IncF, and IncX incompatibility groups. Conserved and variable regions exist in each plasmid, with resistance genes located in clusters within the variable regions. Addiction systems were found in most plasmids to ensure their presence in the cell.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Multidisciplinary
Wang Wen-jing, Wang Yi-fu, Jin Ya-jie, Song Wu-qiang, Lin Jia-meng, Zhang Yan, Tong Xin-ru, Tu Jian, Li Rui-chao, Li Tao
Summary: An extensively drug-resistant (XDR) Escherichia coli strain 258E was identified in an anal swab sample from a chicken farm in Anhui Province, China. The strain 258E carries an IncN plasmid pEC258-3, which contains multiple resistance genes and is highly similar to a plasmid found in Klebsiella pneumoniae. Conjugation experiments confirm the horizontal transferability of plasmid pEC258-3, posing a significant potential threat in animals, community, and hospital settings.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Infectious Diseases
Ji-Hyun Choi, Md. Sekendar Ali, Bo-Youn Moon, Hee-Young Kang, Su-Jeong Kim, Hyun-Ju Song, Abraham Fikru Mechesso, Dong-Chan Moon, Suk-Kyung Lim
Summary: A total of 836 Escherichia coli isolates were recovered from dogs and cats between 2018 and 2019, with 17.1% and 21.2% of isolates resistant to cefovecin and enrofloxacin, respectively. Higher resistance rates were observed in dog isolates compared to cat isolates. The detection of the ST131 clone carrying bla(CTX-M-14/15) in companion animals poses a public health threat.
Article
Multidisciplinary Sciences
Songwei Ni, Baiyuan Li, Kaihao Tang, Jianyun Yao, Thomas K. Wood, Pengxia Wang, Xiaoxue Wang
Summary: The study reveals that the type II TA system, Pseudoalteromonas rubra plasmid toxin-antitoxin PrpT/PrpA, directly controls plasmid replication by acting as a negative regulator. The antitoxin PrpA binds to iterons in the plasmid origin to inhibit the binding of the replication initiator, preventing overreplication of the plasmid. This TA system is conserved and widespread on many conjugative plasmids.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Matthew T. Bird, David R. Greig, Satheesh Nair, Claire Jenkins, Gauri Godbole, Saheer E. Gharbia
Summary: This study used sequencing to determine the origins and transmission mechanisms of antimicrobial resistance in Escherichia coli causing travellers' diarrhoea, providing valuable insights into the spread of resistant genes in bacterial populations.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Lili Li, Rikke Heidemann Olsen, Jian Xiao, Hecheng Meng, Shifu Peng, Lei Shi
Summary: This study is the first to report a multidrug-resistant Salmonella enterica serovar Saintpaul isolate recovered from fish in China that harbors resistance genes to fluoroquinolones and extended-spectrum cephalosporins. The study identified the presence of acquired antibiotic resistance genes and mobile genetic elements on a plasmid, as well as the transferability of these resistance genes to Escherichia coli. Genome sequence comparisons linked the isolate with strains from the United Kingdom.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Vincent van Almsick, Franziska Schuler, Alexander Mellmann, Vera Schwierzeck
Summary: This study presents whole genome sequencing data of two multidrug resistant bacteria isolates, showing the horizontal plasmid exchange between the two species, which explains their correlated resistance phenotypes.
Review
Biochemistry & Molecular Biology
Izabela Kern-Zdanowicz
Summary: pCTX-M3 is the archetype of IncM incompatibility group of conjugative plasmids responsible for worldwide dissemination of antibiotic resistance genes and has a limited host range within Enterobacteriaceae, while its conjugation system has a wide range of recipients including Alpha-, Beta-, and Gammaproteobacteria, and Firmicutes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Yan Zhang, Yin-Huan Sun, Jiang-Yang Wang, Man-Xia Chang, Qiu-Yun Zhao, Hong-Xia Jiang
Summary: The study elucidated the prevalence of bla(CTX-M-27)-producing Escherichia coli and transmission mechanisms in swine farms in China. The main mechanism of transmission was through plasmids, with a novel structure identified that increased the stability of the bla(CTX-M-27) gene. Over time, the predominant vehicle for bla(CTX-M-27) transmission has diversified.
Article
Microbiology
Zhiyuan He, Sirui Yang, Yulin Ma, Shuyuan Zhang, Zhijun Cao
Summary: Newborn dairy calves are often colonized by multidrug-resistant ESBL-EC, with dairy colostrum potentially serving as a source of these resistant bacteria. The study found similar antibiotic resistance patterns in E. coli strains isolated from colostrum fed to dairy calves and their feces. The detection of ESBL-EC strains carrying bla(CTX-M-15) and bla(TEM-171) genes, isolated from feces and colostrum fed to the calves, represents a novel finding.
Article
Genetics & Heredity
Aya Takashima, Hibiki Kawano, Tomomi Ueda, Chiho Suzuki-Minakuchi, Kazunori Okada, Hideaki Nojiri
Summary: The study identified a TA system (prcA/T) that can improve the stability of unstable plasmids in P. resinovorans and E. coli strains.
Article
Chemistry, Multidisciplinary
Zhigang Yu, Emily C. A. Goodall, Ian R. R. Henderson, Jianhua Guo
Summary: This study reveals that plasmid-free bacterial cells exhibit filamentation and asymmetrical cell division under exposure to sub-inhibitory concentrations of antibiotics, while plasmid-bearing cells do not show such morphological changes. Higher expression of sulA is observed in plasmid-free cells. The benefits of plasmid carriage include less DNA damage, stronger efflux pump activities, and higher antibiotic tolerance.
Article
Medicine, Research & Experimental
Christopher O. Fakorede, Kehinde O. Amisu, Morteza Saki, Kabiru O. Akinyemi
Summary: This study investigated antibiotic resistance and the carriage of bla(CTX-M-9) and bla(CTX-M-15) among Salmonella species from hospitalised patients in Lagos, Nigeria. It found a high level of resistance to cephalosporins, penicillin, and some carbapenems, indicating the need for prudent use of cephalosporins.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Immunology
Chongmei Tian, Mengyu Xing, Yaping Zhao, Xueyu Fan, Yongfeng Bai, Liping Fu, Siwei Wang
Summary: This study identified a carbapenem-resistant Klebsiella pneumoniae strain producing OXA-232 with chromosomal bla(CTX-M-15) and virulence plasmid, mainly isolated from China and showing evolutionary trends in recent years. Early detection of this high-risk clone is recommended to prevent its widespread dissemination.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Vincenzo Di Pilato, Romina Papa-Ezdra, Adriana Chiarelli, Virginia Garcia-Fulgueiras, Lucia Pallecchi, Rafael Vignoli
Article
Infectious Diseases
Romina Papa-Ezdra, Fabio Grill Diaz, Mariela Vieytes, Virginia Garcia-Fulgueiras, Leticia Caiata, Pablo Avila, Maria Brasesco, Ines Christophersen, Nicolas F. Cordeiro, Gabriela Algorta, Antonio Galiana, Rafael Vignoli
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2020)
Article
Infectious Diseases
Paula Da Cunda, Victoria Iribarnegaray, Romina Papa-Ezdra, Ines Bado, Maria Jose Gonzalez, Pablo Zunino, Rafael Vignoli, Paola Scavone
MICROBIAL DRUG RESISTANCE
(2020)
Article
Microbiology
Virginia Garcia-Fulgueiras, Yuliana Zapata, Romina Papa-Ezdra, Pablo Avila, Leticia Caiata, Veronica Seija, Ana E. Rojas Rodriguez, Carmen Magallanes, Carolina Marquez Villalba, Rafael Vignoli
REVISTA ARGENTINA DE MICROBIOLOGIA
(2020)
Letter
Infectious Diseases
Nicolas F. Cordeiro, Andres Iriarte, Lucia Yim, Laura Betancor, Jose Alejandro Chabalgoity, Teresa Camou, Rafael Vignoli
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2020)
Article
Infectious Diseases
Virginia Garcia-Fulgueiras, Carmen Magallanes, Victoria Reyes, Camila Cayota, Antonio Galiana, Mariela Vieytes, Rafael Vignoli, Carolina Marquez
Summary: The study investigated genetic relatedness between three isolates of K. pneumoniae from the same patient, showing they all belonged to the same clone, carried various resistance genes, and exhibited different susceptibility profiles. The findings demonstrate the potential plasticity of the ST258 K. pneumoniae clone and describe the first instance of bla(NDM-1)/bla(KPC-2)-producing K. pneumoniae ST258 in Latin America.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Veterinary Sciences
Nadia Coppola, Bibiana Freire, Ana Umpierrez, Nicolas F. Cordeiro, Pablo Avila, Gustavo Trenchi, Gustavo Castro, Maria Laura Casaux, Martin Fraga, Pablo Zunino, Ines Bado, Rafael Vignoli
FRONTIERS IN VETERINARY SCIENCE
(2020)
Article
Infectious Diseases
R. Papa-Ezdra, L. Caiata, R. Palacio, M. Outeda, L. Cabezas, A. Balsamo, R. Vignoli, I Bado, V Seija
Summary: This study aimed to characterize carbapenemase-producing enterobacteria (CPE) isolates obtained from an outbreak-free setting in Uruguay. The results showed that NDM-1, VIM-2, and KPC-2 were the most prevalent carbapenemases among the isolates, which were resistant to most antibiotics but susceptible to fosfomycin. Association between various resistance genes and their presence in transferable plasmids were observed, raising concerns about the coexistence of multiple resistance mechanisms.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Infectious Diseases
R. Papa-Ezdra, N. F. Cordeiro, V. Di Pilato, A. Chiarelli, L. Pallecchi, V. Garcia-Fulgueiras, R. Vignoli
Summary: This study sequenced two IncA/C plasmids carrying bla(CTX-M-2) in Klebsiella pneumoniae clinical isolates and identified complex transposons mediating resistance to multiple antibiotics. The plasmids confer resistance to oxyimino-cephalosporins, gentamicin, and chloramphenicol. The findings contribute to a better understanding of the resistance islands in these plasmids.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Editorial Material
Veterinary Sciences
Josefina Leon-Felix, Gabriel Gutkind, Gabriel Arriagada, Rafael Vignoli
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Infectious Diseases
Romina Papa-Ezdra, Nicolas F. Cordeiro, Matilde Outeda, Virginia Garcia-Fulgueiras, Lucia Araujo, Veronica Seija, Juan A. Ayala, Ines Bado, Rafael Vignoli
Summary: This study describes two novel resistance regions responsible for conferring multidrug resistance phenotype to two clinical isolates of carbapenem-resistant Pseudomonas aeruginosa. Both isolates were resistant to multiple antibiotics and the resistance regions were flanked by inverted repeats. It is important to monitor the emergence of such genetic structures to preserve therapeutic resources.
Article
Infectious Diseases
Papa-Ezdra Romina, Araujo Lucia, Caiata Leticia, Ferreira Federica, Avila Pablo, Seija Veronica, Galiana Antonio, Bado Ines, Vignoli Rafael
Summary: This work describes the in vitro performance of the combined activity of ceftazidime-avibactam and aztreonam against carbapenemase-producing Enterobacterales. The study shows that the combination of ceftazidime-avibactam and aztreonam has a synergistic effect.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Veterinary Sciences
Ana Umpierreza, Deborah Ernst, Andrea Cardozo, Alexia Torres, Magali Fernandez, Martin Fraga, Rafael Vignoli, Ines Bado, Roberto Vidal, Pablo Zunino
Summary: The study aimed to investigate the characteristics of Shiga toxin-producing Escherichia coli (STEC) from calf origin and found differences in genotypes, serogroups, and antibiotic susceptibility in non-O157 STEC isolates. The results of the study suggest that non-O157 STEC pose a potential risk to humans in terms of their virulence profiles and serogroups.
AUSTRAL JOURNAL OF VETERINARY SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Nadia Coppola, Nicolas F. Cordeiro, Gustavo Trenchi, Fernanda Esposito, Bruna Fuga, Danny Fuentes-Castillo, Nilton Lincopan, Andres Iriarte, Ines Bado, Rafael Vignoli
Summary: This study investigates the presence of priority pathogens carrying transferable resistance genes in 1-day-old chickens imported from Brazil to Uruguay, highlighting the potential role of imported chicks in the dissemination of multidrug-resistant pathogens and the spread of antimicrobial resistance.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Nicolas F. Cordeiro, Bruno D'Alessandro, Andres Iriarte, Derek Pickard, Lucia Yim, Jose Alejandro Chabalgoity, Laura Betancor, Rafael Vignoli
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2018)