期刊
GENES
卷 8, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/genes8010034
关键词
site-directed RNA editing; ADAR; guideRNA; genetic disease; RNA repair
资金
- University of Tubingen
- Deutsche Forschungsgemeinschaft [STA 1053/3-2]
- European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program [647328]
- European Research Council (ERC) [647328] Funding Source: European Research Council (ERC)
Site-directed RNA editing is an approach to reprogram genetic information at the RNA level. We recently introduced a novel guideRNA that allows for the recruitment of human ADAR2 to manipulate genetic information. Here, we show that the current guideRNA design is already able to recruit another human deaminase, ADAR1, in both isoforms, p110 and p150. However, further optimization seems necessary as the current design is less efficient for ADAR1 isoforms. Furthermore, we describe hotspots at which the guideRNA itself is edited and show a way to circumvent this auto-editing without losing editing efficiency at the target. Both findings are important for the advancement of site-directed RNA editing as a tool in basic biology or as a platform for therapeutic editing.
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