期刊
FEBS OPEN BIO
卷 7, 期 3, 页码 358-366出版社
WILEY
DOI: 10.1002/2211-5463.12179
关键词
cotton fiber; fast cell growth; phosphorylation; pyruvate kinase; ubiquitination
资金
- State Key Basic Research and Development Plan [2010CB126003]
- National Transgenic Animals and Plants Research Projec [2011ZX08005-003, 2011ZX08009-003]
- China Postdoctoral Science Foundation [2014M550074]
- State Key Laboratory of Cotton Biology Open Fund [CB2015A01]
Pyruvate kinase (PK, EC 2.7.1.40) is an important glycolytic enzyme involved in multiple physiological and developmental processes. In this study, we demonstrated that cotton cytosolic pyruvate kinase 6 (GhPK6) was phosphorylated at serines 215 and 402. Phosphorylation of GhPK6 at serine 215 inhibited its enzyme activity, whereas phosphorylation at both serine sites could promote its degradation. The phosphorylation-mediated ubiquitination of GhPK6 was gradually attenuated during the cotton fiber elongation process, which sufficiently explained the increase in the protein/mRNA ratios. These results collectively provided experimental evidence that cotton fiber elongation might be regulated at the post-translational level.
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