期刊
CHEMELECTROCHEM
卷 5, 期 3, 页码 429-433出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/celc.201700983
关键词
microarray; DNA sequencing; microRNA; cancer diagnosis; blood test; electrochemical analysis
资金
- NIH/NCI [CA186788-01, CA199058-01]
- Nebraska Department of Economic Development [16-01-171, 15-01-055]
Circulating microRNA are promising diagnostic and prognostic biomarkers of disease in quantitative blood tests. A label-free, PCR-free, electrochemical microarray technology on a monolith electrode is described, with 10 attomolar (aM) sensitivity and responsiveness to binding of <1 zeptomole of target to immobilized ssDNA probes with zero background. Specificity is 100% in a mixture with five nonspecific miRNA each with a 10(3)-fold higher concentration. Direct measurement on plasma-derived miRNA without cDNA conversion and PCR demonstrated multiplexing and near-ideal quantitative correlation with an equivalent pure sample. The dynamic range is a target concentration ranging from 10(-2) to 10(3) femtomolar (fM). This PCR-free novel technology can be applied as a test for cancer diagnosis/prognosis to detect 10(3) copies of a miRNA sequence in RNA extracted from 100 mu L of plasma.
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