Old Yellow Enzyme-Catalysed Asymmetric Hydrogenation: Linking Family Roots with Improved Catalysis

A thermophilic-like ene-reductase originating from an acidophilic iron oxidizer

(2016) Anika Scholtissek et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A survey of synthetic nicotinamide cofactors in enzymatic processes

(2016) Caroline E. Paul et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Characterization of an ene-reductase from Meyerozyma guilliermondii for asymmetric bioreduction of α,β-unsaturated compounds

(2016) Baoqi Zhang et al.   BIOTECHNOLOGY LETTERS

Loop-Grafted Old Yellow Enzymes in the Bienzymatic Cascade Reduction of Allylic Alcohols

(2016) Sabrina Reich et al.   CHEMBIOCHEM

Two “classical” Old Yellow Enzymes from Chryseobacterium sp. CA49: Broad substrate specificity of Chr-OYE1 and limited activity of Chr-OYE2

(2016) Xiao-Qiong Pei et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Identification and characterization of a novel Old Yellow Enzyme from Bacillus subtilis str.168

(2016) Xiqian Sheng et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Better than Nature: Nicotinamide Biomimetics That Outperform Natural Coenzymes

(2016) Tanja Knaus et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Donor–Acceptor Distance Sampling Enhances the Performance of “Better than Nature” Nicotinamide Coenzyme Biomimetics

(2016) Alexander Geddes et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The NADH:flavin oxidoreductase Nox fromRhodococcus erythropolisMI2 is the key enzyme of 4,4′-dithiodibutyric acid degradation

(2016) H. Khairy et al.   LETTERS IN APPLIED MICROBIOLOGY

Efficient In Situ Regeneration of NADH Mimics by an Artificial Metalloenzyme

(2016) Yasunori Okamoto et al.   ACS Catalysis

Light-driven biocatalytic reduction of α,β-unsaturated compounds by ene reductases employing transition metal complexes as photosensitizers

(2016) Martyn K. Peers et al.   Catalysis Science & Technology

Opposite Enantioselectivity in the Bioreduction of (Z )-β-Aryl-β-cyanoacrylates Mediated by the Tryptophan 116 Mutants of Old Yellow Enzyme 1: Synthetic Approach to (R )- and (S )-β-Aryl-γ-lactams

(2015) Elisabetta Brenna et al.   ADVANCED SYNTHESIS & CATALYSIS

Enzymatic Synthesis of Optically Active Lactones via Asymmetric Bioreduction using Ene-Reductases from the Old Yellow Enzyme Family

(2015) Nikolaus G. Turrini et al.   ADVANCED SYNTHESIS & CATALYSIS

Applications of protein engineering to members of the old yellow enzyme family

(2015) Erica D. Amato et al.   BIOTECHNOLOGY ADVANCES

The Organic-Synthetic Potential of Recombinant Ene Reductases: Substrate-Scope Evaluation and Process Optimization

(2015) Tina Reß et al.   ChemCatChem

Substrate Scope Evaluation of the Enantioselective Reduction of β-Alkyl-β-arylnitroalkenes by Old Yellow Enzymes 1-3 for Organic Synthesis Applications

(2015) Mattia Bertolotti et al.   ChemCatChem

Reduction of α,β-Unsaturated Ketones by Old Yellow Enzymes: Mechanistic Insights from Quantum Mechanics/Molecular Mechanics Calculations

(2015) Richard Lonsdale et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Functional characterization and stability improvement of a ‘thermophilic-like’ ene-reductase from Rhodococcus opacus 1CP

(2015) Anika Riedel et al.   Frontiers in Microbiology

Nitrile as Activating Group in the Asymmetric Bioreduction of β-Cyanoacrylic Acids Catalyzed by Ene-Reductases

(2014) Christoph K. Winkler et al.   ADVANCED SYNTHESIS & CATALYSIS

Isolation and characterization of a thermotolerant ene reductase from Geobacillus sp. 30 and its heterologous expression in Rhodococcus opacus

(2014) Naoto Tsuji et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Pichia stipitis OYE 2.6 variants with improved catalytic efficiencies from site-saturation mutagenesis libraries

(2014) Athéna Patterson-Orazem et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Identification, Characterization, and Application of Three Enoate Reductases from Pseudomonas putida in In Vitro Enzyme Cascade Reactions

(2014) Christin Peters et al.   ChemCatChem

New developments in ‘ene’-reductase catalysed biological hydrogenations

(2014) Helen S Toogood et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

An ene reductase from Clavispora lusitaniae for asymmetric reduction of activated alkenes

(2014) Yan Ni et al.   ENZYME AND MICROBIAL TECHNOLOGY

Identification and characterization of a novel “thermophilic-like” Old Yellow Enzyme from the genome of Chryseobacterium sp. CA49

(2014) Meng-Yu Xu et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Comparative Structural Modeling of Six Old Yellow Enzymes (OYEs) from the Necrotrophic Fungus Ascochyta rabiei : Insight into Novel OYE Classes with Differences in Cofactor Binding, Organization of Active Site Residues and Stereopreferences

(2014) Shadab Nizam et al.   PLoS One

Engineering of Flexible Loops in Enzymes

(2014) Bettina M. Nestl et al.   ACS Catalysis

Residues Controlling Facial Selectivity in an Alkene Reductase and Semirational Alterations to Create Stereocomplementary Variants

(2014) Adam Z. Walton et al.   ACS Catalysis

Is Simpler Better? Synthetic Nicotinamide Cofactor Analogues for Redox Chemistry

(2014) Caroline E. Paul et al.   ACS Catalysis

Comprehensive genome-wide analysis reveals different classes of enigmatic old yellow enzyme in fungi

(2014) Shadab Nizam et al.   Scientific Reports

An enoate reductase Achr-OYE4 from Achromobacter sp. JA81: characterization and application in asymmetric bioreduction of C=C bonds

(2013) Hai-Bo Wang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Overcoming co-product inhibition in the nicotinamide independent asymmetric bioreduction of activated CC-bonds using flavin-dependent ene-reductases

(2013) Christoph K. Winkler et al.   BIOTECHNOLOGY AND BIOENGINEERING

Alternative Hydride Sources for Ene-Reductases: Current Trends

(2013) Helen S. Toogood et al.   ChemCatChem

NAD(P)H-Independent Asymmetric CC Bond Reduction Catalyzed by Ene Reductases by Using Artificial Co-substrates as the Hydrogen Donor

(2013) Christoph K. Winkler et al.   CHEMISTRY-A EUROPEAN JOURNAL

On the development of new biocatalytic processes for practical pharmaceutical synthesis

(2013) Gjalt W Huisman et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Unusual reactions mediated by FMN-dependent ene- and nitro-reductases

(2013) Katharina Durchschein et al.   GREEN CHEMISTRY

Heterologous expression and characterization of the ene-reductases from Deinococcus radiodurans and Ralstonia metallidurans

(2013) S. Litthauer et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Chemoenzymatic Asymmetric Synthesis of Pregabalin Precursors via Asymmetric Bioreduction of β-Cyanoacrylate Esters Using Ene-Reductases

(2013) Christoph K. Winkler et al.   JOURNAL OF ORGANIC CHEMISTRY

Variations in the stability of NCR ene reductase by rational enzyme loop modulation

(2013) Sabrina Reich et al.   JOURNAL OF STRUCTURAL BIOLOGY

X-ray Crystallography Reveals How Subtle Changes Control the Orientation of Substrate Binding in an Alkene Reductase

(2013) Yuri A. Pompeu et al.   ACS Catalysis

Stereochemical Outcome of the Biocatalysed Reduction of Activated Tetrasubstituted Olefins by Old Yellow Enzymes 1-3

(2012) Elisabetta Brenna et al.   ADVANCED SYNTHESIS & CATALYSIS

Ene Reductase-Catalysed Synthesis of (R)-Profen Derivatives

(2012) Jörg Pietruszka et al.   ADVANCED SYNTHESIS & CATALYSIS

Key Enzymes Enabling the Growth of Arthrobacter sp. Strain JBH1 with Nitroglycerin as the Sole Source of Carbon and Nitrogen

(2012) Johana Husserl et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Asymmetric bioreduction of activated alkenes by a novel isolate of Achromobacter species producing enoate reductase

(2012) Yan-Jie Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Protein design at the interface of the pre-biotic and biotic worlds

(2012) Liam M. Longo et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Comparative characterization of novel ene-reductases from cyanobacteria

(2012) Yilei Fu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Revisiting the Enantioselective Sequence Patterns in Enoate Reductases

(2012) Thomas Classen et al.   ChemCatChem

A Substrate-Driven Approach to Determine Reactivities of α,β-Unsaturated Carboxylic Esters Towards Asymmetric Bioreduction

(2012) Gábor Tasnádi et al.   CHEMISTRY-A EUROPEAN JOURNAL

Biochemical characterization and substrate profiling of a new NADH-dependent enoate reductase from Lactobacillus casei

(2012) Xiuzhen Gao et al.   ENZYME AND MICROBIAL TECHNOLOGY

Nicotinamide-Dependent Ene Reductases as Alternative Biocatalysts for the Reduction of Activated Alkenes

(2012) Katharina Durchschein et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Asymmetric bioreduction of activated alkenes to industrially relevant optically active compounds

(2012) Christoph K. Winkler et al.   JOURNAL OF BIOTECHNOLOGY

Mimicking Nature: Synthetic Nicotinamide Cofactors for C═C Bioreduction Using Enoate Reductases

(2012) Caroline E. Paul et al.   ORGANIC LETTERS

A novel ene-reductase from Synechococcus sp. PCC 7942 for the asymmetric reduction of alkenes

(2012) Yilei Fu et al.   PROCESS BIOCHEMISTRY

Reductive dehalogenation of β-haloacrylic ester derivatives mediated by ene-reductases

(2012) Gábor Tasnádi et al.   Catalysis Science & Technology

Old Yellow Enzyme-Catalyzed Dehydrogenation of Saturated Ketones

(2011) Matthias Schittmayer et al.   ADVANCED SYNTHESIS & CATALYSIS

A highly efficient ADH-coupled NADH-recycling system for the asymmetric bioreduction of carbon-carbon double bonds using enoate reductases

(2011) Katharina Tauber et al.   BIOTECHNOLOGY AND BIOENGINEERING

Stereopreferences of Old Yellow Enzymes: Structure Correlations and Sequence Patterns in Enoate Reductases

(2011) Gustav Oberdorfer et al.   ChemCatChem

Biocatalytic Reductions of Baylis–Hillman Adducts

(2011) Adam Z. Walton et al.   ACS Catalysis

The Substrate Spectra of Pentaerythritol Tetranitrate Reductase, Morphinone Reductase,N-Ethylmaleimide Reductase and Estrogen-Binding Protein in the Asymmetric Bioreduction of Activated Alkenes

(2010) Nicole J. Mueller et al.   ADVANCED SYNTHESIS & CATALYSIS

Asymmetric reduction of activated alkenes using an enoate reductase from Gluconobacter oxydans

(2010) Nina Richter et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Crystal structure of a thermostable Old Yellow Enzyme from Thermus scotoductus SA-01

(2010) Diederik J. Opperman et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Biocatalytic Reductions and Chemical Versatility of the Old Yellow Enzyme Family of Flavoprotein Oxidoreductases

(2010) Helen S. Toogood et al.   ChemCatChem

Towards preparative-scale, biocatalytic alkene reductions

(2010) Despina J. Bougioukou et al.   CHEMICAL COMMUNICATIONS

Biocatalysis—key to sustainable industrial chemistry

(2010) Roland Wohlgemuth   CURRENT OPINION IN BIOTECHNOLOGY

Determinants of Substrate Binding and Protonation in the Flavoenzyme Xenobiotic Reductase A

(2010) Olivia Spiegelhauer et al.   JOURNAL OF MOLECULAR BIOLOGY

Directed Evolution of an Enantioselective Enoate-Reductase: Testing the Utility of Iterative Saturation Mutagenesis

(2009) Despina J. Bougioukou et al.   ADVANCED SYNTHESIS & CATALYSIS

Asymmetric Reduction of Activated Alkenes by Pentaerythritol Tetranitrate Reductase: Specificity and Control of Stereochemical Outcome by Reaction Optimisation

(2009) Anna Fryszkowska et al.   ADVANCED SYNTHESIS & CATALYSIS

Kinetic Characterization of Xenobiotic Reductase A fromPseudomonas putida86

(2009) Olivia Spiegelhauer et al.   BIOCHEMISTRY

Biocatalysis with Thermostable Enzymes: Structure and Properties of a Thermophilic ‘ene’-Reductase related to Old Yellow Enzyme

(2009) Björn V. Adalbjörnsson et al.   CHEMBIOCHEM

Evidence To Support the Hypothesis That Promoting Vibrations Enhance the Rate of an Enzyme Catalyzed H-Tunneling Reaction

(2009) Christopher R Pudney et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-Saturation Mutagenesis of Tryptophan 116 of Saccharomyces pastorianus Old Yellow Enzyme Uncovers Stereocomplementary Variants

(2009) Santosh Kumar Padhi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Nicotinamide-independent asymmetric bioreduction of CC-bonds via disproportionation of enones catalyzed by enoate reductases

(2009) Clemens Stueckler et al.   TETRAHEDRON

Asymmetric Bioreduction of CC Bonds using Enoate Reductases OPR1, OPR3 and YqjM: Enzyme-Based Stereocontrol

(2008) Mélanie Hall et al.   ADVANCED SYNTHESIS & CATALYSIS

Structure-Based Insight into the Asymmetric Bioreduction of the CC Double Bond of α,β-Unsaturated Nitroalkenes by Pentaerythritol Tetranitrate Reductase

(2008) Helen S. Toogood et al.   ADVANCED SYNTHESIS & CATALYSIS

Asymmetric Bioreduction of Activated C=C Bonds UsingZymomonas mobilis NCR Enoate Reductase and Old Yellow Enzymes OYE 1–3 from Yeasts

(2008) Mélanie Hall et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Reduction of aliphatic nitroesters and N-nitramines by Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase

(2008) Henrikas Nivinskas et al.   FEBS Journal

A Novel Chromate Reductase from Thermus scotoductus SA-01 Related to Old Yellow Enzyme

(2008) D. J. Opperman et al.   JOURNAL OF BACTERIOLOGY