Targeted genome editing in Caenorhabditis elegans using CRISPR/Cas9

Diversity and evolution of class 2 CRISPR–Cas systems

(2017) Sergey Shmakov et al.   NATURE REVIEWS MICROBIOLOGY

Characterization of the interplay between DNA repair and CRISPR/Cas9-induced DNA lesions at an endogenous locus

(2017) Anne Bothmer et al.   Nature Communications

One-step homozygosity in precise gene editing by an improved CRISPR/Cas9 system

(2016) Pei Zhao et al.   CELL RESEARCH

CRISPR/Cas9 Mutagenesis Reveals Versatile Roles of Hox Genes in Crustacean Limb Specification and Evolution

(2016) Arnaud Martin et al.   CURRENT BIOLOGY

Functional Conservation and Divergence ofdaf-22Paralogs inPristionchus pacificusDauer Development

(2016) Gabriel V. Markov et al.   MOLECULAR BIOLOGY AND EVOLUTION

New CRISPR–Cas systems from uncultivated microbes

(2016) David Burstein et al.   NATURE

Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA

(2016) Christopher D Richardson et al.   NATURE BIOTECHNOLOGY

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Empower multiplex cell and tissue-specific CRISPR-mediated gene manipulation with self-cleaving ribozymes and tRNA

(2016) Li Xu et al.   NUCLEIC ACIDS RESEARCH

Distinct patterns of Cas9 mismatch tolerancein vitroandin vivo

(2016) Becky X.H. Fu et al.   NUCLEIC ACIDS RESEARCH

CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci

(2016) Yi Fu et al.   Nature Communications

Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9

(2016) Satoru Iwata et al.   Scientific Reports

Polymerase θ is a robust terminal transferase that oscillates between three different mechanisms during end-joining

(2016) Tatiana Kent et al.   eLife

Nucleosome breathing and remodeling constrain CRISPR-Cas9 function

(2016) R Stefan Isaac et al.   eLife

Nucleosomes impede Cas9 access to DNA in vivo and in vitro

(2016) Max A Horlbeck et al.   eLife

Nucleosomes Inhibit Cas9 Endonuclease Activity in Vitro

(2015) John M. Hinz et al.   BIOCHEMISTRY

CRISPR-ERA: a comprehensive design tool for CRISPR-mediated gene editing, repression and activation: Fig. 1.

(2015) Honglei Liu et al.   BIOINFORMATICS

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Regulation of transcriptionally active genes via the catalytically inactive Cas9 in C. elegans and D. rerio

(2015) Lijiang Long et al.   CELL RESEARCH

Anillin Regulates Neuronal Migration and Neurite Growth by Linking RhoG to the Actin Cytoskeleton

(2015) Dong Tian et al.   CURRENT BIOLOGY

Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans

(2015) R. T. Bell et al.   GENETICS

Computer-Assisted Transgenesis of Caenorhabditis elegans for Deep Phenotyping

(2015) C. L. Gilleland et al.   GENETICS

Efficient Genome Editing inCaenorhabditis eleganswith a Toolkit of Dual-Marker Selection Cassettes

(2015) Adam D. Norris et al.   GENETICS

Dramatic Enhancement of Genome Editing by CRISPR/Cas9 Through Improved Guide RNA Design

(2015) Behnom Farboud et al.   GENETICS

Targeted Chromosomal Translocations and Essential Gene Knockout Using CRISPR/Cas9 Technology in Caenorhabditis elegans

(2015) X. Chen et al.   GENETICS

High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes

(2015) A. Paix et al.   GENETICS

Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette

(2015) Daniel J. Dickinson et al.   GENETICS

Somatic CRISPR–Cas9-induced mutations reveal roles of embryonically essential dynein chains inCaenorhabditis eleganscilia

(2015) Wenjing Li et al.   JOURNAL OF CELL BIOLOGY

Crescerin uses a TOG domain array to regulate microtubules in the primary cilium

(2015) Alakananda Das et al.   MOLECULAR BIOLOGY OF THE CELL

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

(2015) Miguel A Moreno-Mateos et al.   NATURE METHODS

Polymerase Θ is a key driver of genome evolution and of CRISPR/Cas9-mediated mutagenesis

(2015) Robin van Schendel et al.   Nature Communications

Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies

(2015) Xueyan Li et al.   Nature Communications

Development of a mono-promoter-driven CRISPR/Cas9 system in mammalian cells

(2015) Shin Yoshioka et al.   Scientific Reports

CRISPR/Cas9 Genome Editing in Caenorhabditis elegans : Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair

(2015) Iskra Katic et al.   G3-Genes Genomes Genetics

Efficient Genome Editing inCaenorhabditis eleganswith a Toolkit of Dual-Marker Selection Cassettes

(2015) Adam D. Norris et al.   GENETICS

Dramatic Enhancement of Genome Editing by CRISPR/Cas9 Through Improved Guide RNA Design

(2015) Behnom Farboud et al.   GENETICS

Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette

(2015) Daniel J. Dickinson et al.   GENETICS

A Candida albicans CRISPR system permits genetic engineering of essential genes and gene families

(2015) V. K. Vyas et al.   Science Advances

Genome Engineering with Targetable Nucleases

(2014) Dana Carroll   Annual Review of Biochemistry

A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging

(2014) Marvin E. Tanenbaum et al.   CELL

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

(2014) Luke A. Gilbert et al.   CELL

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Oligonucleotide-based targeted gene editing in C. elegans via the CRISPR/Cas9 system

(2014) Pei Zhao et al.   CELL RESEARCH

Heritable/conditional genome editing in C. elegans using a CRISPR-Cas9 feeding system

(2014) Pengpeng Liu et al.   CELL RESEARCH

Gene inactivation using the CRISPR/Cas9 system in the nematode Pristionchus pacificus

(2014) Hanh Witte et al.   DEVELOPMENT GENES AND EVOLUTION

Conditional Knockouts Generated by Engineered CRISPR-Cas9 Endonuclease Reveal the Roles of Coronin in C. elegans Neural Development

(2014) Zhongfu Shen et al.   DEVELOPMENTAL CELL

Scalable and Versatile Genome Editing Using Linear DNAs with Microhomology to Cas9 Sites in Caenorhabditis elegans

(2014) Alexandre Paix et al.   GENETICS

Rapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-Conversion and Inactivation of NHEJ Repair

(2014) Jordan D. Ward   GENETICS

A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis elegans

(2014) Heesun Kim et al.   GENETICS

Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans

(2014) J. A. Arribere et al.   GENETICS

Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells

(2014) Lior Nissim et al.   MOLECULAR CELL

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

(2014) Carolin Anders et al.   NATURE

Programmable RNA recognition and cleavage by CRISPR/Cas9

(2014) Mitchell R. O’Connell et al.   NATURE

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

(2014) Xuebing Wu et al.   NATURE BIOTECHNOLOGY

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon

(2014) Christian Frøkjær-Jensen et al.   NATURE METHODS

Landscape of target:guide homology effects on Cas9-mediated cleavage

(2014) Becky Xu Hua Fu et al.   NUCLEIC ACIDS RESEARCH

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

(2014) Tessa G. Montague et al.   NUCLEIC ACIDS RESEARCH

Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs

(2014) James A. Gagnon et al.   PLoS One

Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage

(2014) Regina Cencic et al.   PLoS One

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Implementation of the CRISPR-Cas9 system in fission yeast

(2014) Jake Z. Jacobs et al.   Nature Communications

Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans

(2014) Xiangyang Chen et al.   Scientific Reports

Enhanced Specificity and Efficiency of the CRISPR/Cas9 System with Optimized sgRNA Parameters in Drosophila

(2014) Xingjie Ren et al.   Cell Reports

Selection of chromosomal DNA libraries using a multiplex CRISPR system

(2014) Owen W Ryan et al.   eLife

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

(2013) Baohui Chen et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

(2013) Albert W Cheng et al.   CELL RESEARCH

Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans

(2013) Iskra Katic et al.   GENETICS

Precise and Heritable Genome Editing in Evolutionarily Diverse Nematodes Using TALENs and CRISPR/Cas9 to Engineer Insertions and Deletions

(2013) T.-W. Lo et al.   GENETICS

Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas

(2013) Hui Chiu et al.   GENETICS

CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans

(2013) Selma Waaijers et al.   GENETICS

Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins

(2013) Seung Woo Cho et al.   GENETICS

Self-processing of ribozyme-flanked RNAs into guide RNAsin vitroandin vivofor CRISPR-mediated genome editing

(2013) Yangbin Gao et al.   Journal of Integrative Plant Biology

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Efficient genome editing in zebrafish using a CRISPR-Cas system

(2013) Woong Y Hwang et al.   NATURE BIOTECHNOLOGY

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination

(2013) Daniel J Dickinson et al.   NATURE METHODS

Cas9 as a versatile tool for engineering biology

(2013) Prashant Mali et al.   NATURE METHODS

RNA-guided gene activation by CRISPR-Cas9–based transcription factors

(2013) Pablo Perez-Pinera et al.   NATURE METHODS

CRISPR RNA–guided activation of endogenous human genes

(2013) Morgan L Maeder et al.   NATURE METHODS

Heritable genome editing in C. elegans via a CRISPR-Cas9 system

(2013) Ari E Friedland et al.   NATURE METHODS

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination

(2013) Changchun Chen et al.   NUCLEIC ACIDS RESEARCH

Mechanism of Eukaryotic RNA Polymerase III Transcription Termination

(2013) S. Nielsen et al.   SCIENCE

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Improved Mos1-mediated transgenesis in C. elegans

(2012) Christian Frøkjær-Jensen et al.   NATURE METHODS

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Widespread occurrence of non-canonical transcription termination by human RNA polymerase III

(2011) Andrea Orioli et al.   NUCLEIC ACIDS RESEARCH

The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli

(2011) Rimantas Sapranauskas et al.   NUCLEIC ACIDS RESEARCH

Targeted Genome Editing Across Species Using ZFNs and TALENs

(2011) Andrew J. Wood et al.   SCIENCE

TAL effectors: finding plant genes for disease and defense

(2010) Adam J Bogdanove et al.   CURRENT OPINION IN PLANT BIOLOGY

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

Targeted gene deletions in C. elegans using transposon excision

(2010) Christian Frøkjær-Jensen et al.   NATURE METHODS

Genome editing with engineered zinc finger nucleases

(2010) Fyodor D. Urnov et al.   NATURE REVIEWS GENETICS

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

(2009) J. Boch et al.   SCIENCE

A Simple Cipher Governs DNA Recognition by TAL Effectors

(2009) M. J. Moscou et al.   SCIENCE

Single-copy insertion of transgenes in Caenorhabditis elegans

(2008) Christian Frøkjær-Jensen et al.   NATURE GENETICS