Article
Microbiology
Jens Bo Andersen, Kasper Norskov Kragh, Louise Dahl Hultqvist, Morten Rybtke, Martin Nilsson, Tim Holm Jakobsen, Michael Givskov, Tim Tolker-Nielsen
Summary: A decade of research has shown the importance of c-di-GMP in bacteria, with high levels associated with biofilm formation and low levels with planktonic bacteria. Inducing specific phosphodiesterases in P. aeruginosa can lead to biofilm dispersal.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Immunology
Siying Lin, Shuzhen Chen, Li Li, Huiluo Cao, Ting Li, Ming Hu, Lisheng Liao, Lian-Hui Zhang, Zeling Xu
Summary: This study characterized a uropathogenic Pseudomonas aeruginosa strain, PA_HN002, and found that its high intracellular c-di-GMP content contributed to enhanced biofilm formation. Analysis of the c-di-GMP metabolizing enzymes in PA_HN002 revealed multiple mutations in phosphodiesterases (PDEs), which resulted in a shifted expression profile and elevated intracellular c-di-GMP levels, promoting biofilm production.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Calvin K. Lee, William C. Schmidt, Shanice S. Webster, Jonathan W. Chen, George A. O'Toole, Gerard C. L. Wong
Summary: This study reveals that changes in c-di-GMP levels in bacterial biofilms are not the sole factor determining surface commitment, as oscillations in c-di-GMP levels can propagate across generations. Additionally, the study demonstrates that modulation of c-di-GMP signals and cooperative surface commitment can be influenced by varying the interaction strength between PilO and SadC.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Yu-Ming Cai, Kai-Wei Yu, Ji-Hong Liu, Zhao Cai, Zun-Hao Zhou, Yang Liu, Tian-Fu Wang, Liang Yang
Summary: The c-di-GMP signaling pathways in P. aeruginosa are highly organized and play important roles in the transition between motile and sessile lifestyles. This study identified a phosphodiesterase, PipA, that regulates bacteriophage production, and uncovered a potential signaling circuit for biofilm treatment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jacob Blacutt, Ziyang Lan, Elizabeth M. Cosgriff-Hernandez, Vernita D. Gordon
Summary: This study presents a new method for measuring statistically significant differences in c-di-GMP signaling associated with different PEGDA gel types and the surface-exposed protein PilY1.
ROYAL SOCIETY OPEN SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Fiorenza Rancan, Jana Jurisch, Cemre Guenday, Emre Tuereli, Ulrike Blume-Peytavi, Annika Vogt, Christoph Schaudinn, Nazende Guenday-Tuereli
Summary: The study found that surfactants significantly improved the penetration of PLGA particles in wound tissue and enhanced the antimicrobial efficacy of antibiotics, offering a potential safe and effective option for topical antimicrobial management of chronic wound infections.
Review
Biotechnology & Applied Microbiology
Anming Xu, Xiaoxiao Zhang, Tong Wang, Fengxue Xin, Luyan Z. Ma, Jie Zhou, Weiliang Dong, Min Jiang
Summary: Pseudomonas aeruginosa is known for its excellent ecological flexibility and can survive in diverse niches. The adaptive mutants RSCVs have been frequently isolated from chronic infections, sparking interest in investigating their formation, persistence, and pathogenesis. RSCVs are believed to play a significant role in biofilm formation and high adaptability.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Immunology
Dingbin Li, Li Zhang, Jinhua Liang, Wusheng Deng, Qingjun Wei, Ke Wang
Summary: This study established a rabbit model of septic arthritis caused by Pseudomonas aeruginosa, demonstrating the formation of biofilms in the knee joint cavity. Scanning electron microscopy and PNA-FISH assays were used to observe the biofilm structures and fiber deposition in the synovial membrane. The findings suggest that c-di-GMP plays a key role in biofilm formation in the rabbit models.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Julia E. E. Dreifus, Lindsey O'Neal, Holly M. M. Jacobs, Adithya S. S. Subramanian, P. Lynne Howell, Daniel J. J. Wozniak, Matthew R. R. Parsek
Summary: The DGC SiaD regulates the production of cell-associated EPS Psl through posttranslational mechanisms in Pseudomonas aeruginosa. The Sia system is important for producing low levels of c-di-GMP and maintaining cell-associated Psl in planktonic populations.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Microbiology
Ying Hu, Jeremy Stephen Webb, Shi-qi An
Summary: The rapid emergence of bacterial resistance to antibiotics poses a significant threat to global healthcare systems. Recent research has focused on targeting regulatory pathways, such as the intracellular molecule c-di-GMP, to identify new effective anti-bacterial agents. Altering the cellular concentrations of this molecule has the potential to reduce biofilm formation or increase susceptibility of bacteria to antibiotics.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Derek Fleming, Brandon Niese, Whitni Redman, Emily Vanderpool, Vernita Gordon, Kendra P. Rumbaugh
Summary: The absence of the exopolysaccharides Pel and Psl in Pseudomonas aeruginosa did not significantly affect the severity or wound closure rates of wound infections in a mouse model. However, the size and distribution of biofilm aggregates in the wound tissue were different, and the mutants' ability to survive antibiotic treatment was impaired.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Erik Gerner, Sofia Almqvist, Peter Thomsen, Maria Werthen, Margarita Trobos
Summary: Inhibition of quorum sensing by sodium salicylate in Pseudomonas aeruginosa strains influences biofilm formation and tolerance to silver, making biofilms more susceptible to silver and reducing levels of virulence factors associated with biofilm development. This suggests that sodium salicylate allows antiseptics to become more readily available to cells by reducing cell aggregation in biofilms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Han -Shin Kim, So-Young Ham, Hwa-Soo Ryoo, Do-Hyung Kim, Eun-Tae Yun, Hee-Deung Park, Jeong-Hoon Park
Summary: Biofilms, composed of bacteria and extracellular polymeric substances (EPSs), increase antibiotic resistance by hindering antibiotic transport to bacterial cells. Citrus peel extract from Jeju Island (CPEJ) was found to inhibit biofilm formation without affecting bacterial growth. CPEJ reduced EPS production and increased bacterial motility. These effects were attributed to the reduction in intracellular cyclic di-guanosine monophosphate (c-di-GMP) levels through increased phosphodiesterase activity. This new finding suggests that CPEJ has potential as a biofilm inhibitor in pharmaceutical and industrial applications.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Biomedical
Keren Zhou, Vinay Kammarchedu, Derrick Butler, Pouya Soltan Khamsi, Aida Ebrahimi
Summary: This study developed a flexible electrochemical sensor based on phenazines for early diagnosis of P. aeruginosa infection and stable monitoring of phenazine generation in biofilms.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Microbiology
Zhexian Liu, Sarzana S. Hossain, Zayda Morales Moreira, Cara H. Haney
Summary: Pseudomonas aeruginosa can regulate biofilm formation by synthesizing and catabolizing polyamines. Both putrescine and its biosynthetic precursor, arginine, promote biofilm formation by increasing the level of c-di-GMP in the cells.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chao Huang, Kiera P. Fisher, Sandra S. Hammer, Julia V. Busik
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Microbiology
David B. Needle, Jacqueline L. Marr, Cooper J. Park, Cheryl P. Andam, Annabel G. Wise, Roger K. Maes, Rebecca P. Wilkes, Eman A. Anis, Inga F. Sidor, Dalen Agnew, Julie C. Ellis, Patrick Tate, Abigail Mathewson, Christopher Benton, Robert Gibson
Article
Biology
Joshua T. Smith, Sharlene Amador, Colin J. McGonagle, David Needle, Robert Gibson, Cheryl P. Andam
COMMUNICATIONS BIOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Julia Busik
Summary: This review article discusses the potential benefits of lipid-lowering drugs for the prevention and treatment of diabetic retinopathy. While fibrates have shown improvement in DR, there is a disconnect between their effects in the retina and blood lipid levels. The article also highlights lipid and lipoprotein-independent mechanisms that may play a role in retinal protection.
JOURNAL OF LIPID RESEARCH
(2021)
Article
Endocrinology & Metabolism
Sandra S. Hammer, Cristiano P. Vieira, Delaney McFarland, Maximilian Sandler, Yan Levitsky, Tim F. Dorweiler, Todd A. Lydic, Bright Asare-Bediako, Yvonne Adu-Agyeiwaah, Micheli S. Sielski, Mariana Dupont, Ana Leda Longhini, Sergio Li Calzi, Dibyendu Chakraborty, Gail M. Seigel, Denis A. Proshlyakov, Maria B. Grant, Julia V. Busik
Summary: Intermittent fasting (IF) can effectively prevent microvascular and bone marrow dysfunction in diabetes patients by activating the SIRT1 signaling pathway, aiding in improving metabolic health and visual function impairment.
Review
Endocrinology & Metabolism
Alicia J. Jenkins, Maria B. Grant, Julia Busik
Summary: Clinical studies provide limited and sometimes conflicting evidence on the relationship between circulating lipid levels and the development of diabetic retinopathy. Trials focused on the cardiovascular system suggest that lipid-lowering therapies may have a protective effect, but trials with ocular primary endpoints are still ongoing.
Article
Microbiology
Spencer A. Bruce, Joshua T. Smith, Jennifer L. Mydosh, John Ball, David B. Needle, Robert Gibson, Cheryl P. Andam
Summary: This study provides insights into the phylogenetic relationships and genetic resistance patterns of Staphylococcus pseudintermedius in New England and global contexts. Methicillin-resistant S. pseudintermedius with diverse resistance genes was found in the region, while the clonally expanding ST71 clone was not detected. The study highlights the importance of understanding the evolution and geographic spread of high-risk clones for the health of canine companions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Charles E. Alex, Marta Canuti, Maya S. Schlesinger, Kenneth A. Jackson, David Needle, Claire Jardine, Larissa Nituch, Laura Bourque, Andrew S. Lang, Patricia A. Pesavento
Summary: This study investigated the geographic distribution and genetic diversity of skunk amdoparvovirus (SKAV) in North America. SKAV was found to have a high prevalence in striped skunks and was detected in different locations across North America. The analysis of the viral genome revealed distinct differences in the evolutionary dynamics of the major structural and non-structural proteins. The virus was found in the epithelium of the gastrointestinal tract, urinary tract, and skin, suggesting multiple possible modes of transmission. The endemicity of SKAV and its genetic diversity suggest a long-term association with the host and a risk of cross-species transmission.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Multidisciplinary Sciences
Spencer A. Bruce, Joshua T. Smith, Jennifer L. Mydosh, John Ball, David B. Needle, Robert Gibson, Cheryl P. Andam
Summary: This study investigates the distribution of resistance and virulence genes in methicillin-resistant Staphylococcus aureus (MRSA) among different animal hosts. Whole genome sequencing of S. aureus isolates from various animal species revealed widespread sharing of antibiotic resistance and superantigen genes. The findings provide insights for developing strategies to combat high-risk clones.
SCIENTIFIC REPORTS
(2022)
Editorial Material
Chemistry, Medicinal
Robert B. Abramovitch
FUTURE MEDICINAL CHEMISTRY
(2022)
Article
Microbiology
Mariano Carossino, Fabio Del Piero, Jeongha Lee, David B. Needle, Jonathan M. Levine, Ronald R. Riis, Roger Maes, Annabel G. Wise, Keenan Mullaney, Jacqueline Ferracone, Ingeborg M. Langohr
Summary: Feline infectious peritonitis (FIP) virus is the most common infectious cause of uveitis in cats. The detection rate of viral antigen and/or RNA in FIP-induced uveitis is dependent on the type of inflammatory response. Predominance of plasmacytic inflammation reduces the likelihood of a confirmatory diagnosis through viral detection methods.
Article
Microbiology
Shelby J. Dechow, Jacob J. Baker, Megan Murto, Robert B. Abramovitch
Summary: It is puzzling why Mycobacterium tuberculosis cannot grow on glycerol at acidic pH, as it has a carbon source and oxygen, everything it needs to grow. It has been found that mutations in ppe51 gene promote glycerol uptake at acidic pH and enable growth. However, these mutations exhibit enhanced replication and reduced survival in activated macrophages, supporting the role of pH-dependent slowed growth during macrophage pathogenesis.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Infectious Diseases
John T. Williams, Robert B. Abramovitch
Summary: Mycobacteria species, including pathogenic bacteria like Mycobacterium tuberculosis and Mycobacterium leprae, rely on the essential protein MmpL3 for the transport of mycolic acids and lipids. Many recent studies have explored the function, regulation, and interactions of MmpL3 as a drug target. This review provides new findings and suggests future research directions in the field.
MICROBIAL DRUG RESISTANCE
(2023)
Article
Medicine, General & Internal
Ram Prasad, Yvonne Adu-Agyeiwaah, Jason L. Floyd, Bright Asare-Bediako, Sergio Li Calzi, Dibyendu Chakraborty, Angela Harbour, Aayush Rohella, Julia V. Busik, Qiuhong Li, Maria B. Grant
Summary: Intestinal lymphatic, known as lacteal, plays a critical role in maintaining intestinal homeostasis. This study examines the impact of type 1 diabetes on intestinal lymphatics and circulating lipids, and tests the impact of intervention with ACE-2-expressing probiotics on gut and retinal function.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Microbiology
Ifeanyichukwu E. Eke, John T. Williams, Elizabeth R. Haiderer, Veronica J. Albrecht, Heather M. Murdoch, Bassel J. Abdalla, Robert B. Abramovitch
Summary: Nitro-containing compounds are important agents in controlling tuberculosis. Several nitro-containing compounds have been identified and characterized for their mechanism of action against Mycobacterium tuberculosis. These compounds show different activities, targets, and bactericidal capabilities.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)