Article
Agriculture, Dairy & Animal Science
Manuela Heimann, Mohamed I. Elashry, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: The investigation of mesenchymal stem cells has become an interesting scientific field in regenerative medicine and organ transplantation. This study focused on establishing an adipogenic differentiation protocol suitable for different mammalian species, and found that using rosiglitazone instead of traditional protocols significantly improves cell viability and adipogenic differentiation capacity. The study also revealed differences between species in terms of fat formation and fat-regulating gene expression. This information is essential for selecting a stem cell specification program and improving clinical targeting of obesity and metabolic disorders in veterinary medicine.
Article
Biochemistry & Molecular Biology
Victor J. B. van Santen, Angela P. Bastidas Coral, Jolanda M. A. Hogervorst, Jenneke Klein-Nulend, Astrid D. Bakker
Summary: A new, biologically relevant in vitro 3D-model was developed to study the bone regeneration potential of MSCs. This model is likely more suitable for the screening of novel factors to enhance bone regeneration than standard cell cultures.
Article
Immunology
Jintao Cheng, Yuan Feng, Xiao Feng, Donghao Wu, Xu Lu, Zhihua Rao, Cuiping Li, Nan Lin, Changchang Jia, Qi Zhang
Summary: The immunomodulatory function of mesenchymal stem cells (MSCs) can be improved with the help of small-molecule compounds, leading to enhanced therapeutic effects and stronger anti-inflammatory properties in mouse models.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Physical
Chunhui Sheng, Xiaodong Guo, Zhuqing Wan, Xiaoqiang Bai, Hao Liu, Xiao Zhang, Ping Zhang, Yunsong Liu, Wenyue Li, Yongsheng Zhou, Longwei Lv
Summary: This study investigated the potential of exosomes derived from human adipose-derived stem cells (hASCs) in promoting osteogenic differentiation for osteoporosis treatment. The study identified the key differentially expressed microRNA between two exosomes and their potential target gene. Results showed that exosomes from osteogenic induction medium had a better effect on promoting osteogenic differentiation than those from proliferation medium. Moreover, miR-335-3p inhibitor-optimized exosomes enhanced osteogenic differentiation in vitro and bone density in vivo, suggesting the potential of engineered exosomes in optimizing exosome-based therapy for osteoporosis.
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Nanoscience & Nanotechnology
Gerard Rubi-Sans, Agata Nyga, Elena Rebollo, Soledad Perez-Amodio, Jorge Otero, Daniel Navajas, Miguel A. Mateos-Timoneda, Elisabeth Engel
Summary: The study introduced a novel method to produce mesenchymal cell-derived matrices (CDMs) aimed at mimicking the fibrotic tumor microenvironment, showing that they closely mimic the composition, structure, and mechanical properties of human fibrotic ECM. CDMs have highly reproducible composition and tunable mechanical properties, and exhibit increased doxorubicin resistance in cancer cells following chemotherapeutic treatment in comparison to 3D culture in collagen hydrogels.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Cell Biology
Domenico Aprile, Nicola Alessio, Ibrahim H. Demirsoy, Tiziana Squillaro, Gianfranco Peluso, Giovanni Di Bernardo, Umberto Galderisi
Summary: The study demonstrated that multi-lineage stem cells can be isolated from a mouse model, offering a valid alternative for disease research and cell therapy, and showing potential applications for these cells.
Article
Biochemistry & Molecular Biology
Szu-Hsien Wu, Yu-Ting Liao, Chi-Han Huang, Yi-Chou Chen, En-Rung Chiang, Jung-Pan Wang
Summary: ADSCs tend to spontaneously differentiate into neuron-like cells during high confluence culture, with enhanced expression of neuronal markers and neurotrophins, while BMSCs do not show this tendency. Additionally, ADSCs do not exhibit spontaneous adipogenic and osteogenic differentiation during this process.
Article
Genetics & Heredity
Meysam Ganjibakhsh, Fereshteh Mehraein, Morteza Koruji, Zahra Bashiri
Summary: The differentiation of human adipose-derived MSCs into male germ cells provides a valuable in vitro platform for studying human germ cell development, offering potential clinical application for treating azoospermia in infertile patients.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Lysann M. Kroschwald, Felix Allerdt, Anne Bernhardt, Sandra Rother, Kai Zheng, Iram Maqsood, Norbert Halfter, Christiane Heinemann, Stephanie Moeller, Matthias Schnabelrauch, Michael C. Hacker, Stefan Rammelt, Aldo R. Boccaccini, Vera Hintze
Summary: The study demonstrates that aECM containing BGN can promote osteogenic differentiation of hMSC while delaying cellular growth, with a stimulating effect on ALP activity and calcium deposition. Some donors show a synergistic effect between sGAG and BGN, contributing to bone regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Stine Bangsgaard Hansen, Lisbeth Drozd Hojgaard, Jens Kastrup, Annette Ekblond, Bjarke Follin, Morten Juhl
Summary: In this study, flow cytometric analysis of lymphocyte proliferation was used to quantify the inhibitory effect of MSC. Specific and unspecific stimulation were tested, and it was found that combining multiple donors enhanced the proliferative response. The study emphasizes the importance of developing robust assays for potency assessment.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marta Cadenas-Martin, Francisco Arnalich-Montiel, Maria P. De Miguel
Summary: This study developed a protocol for deriving human limbal stem cells (LSCs) from adipose-derived mesenchymal stem cells (ADSCs) that is compatible with the standard carrier human amniotic membrane. The best protocol achieved optimal differentiation efficiency and will benefit patients with LSC deficiency, aiding in the restoration of vision.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Jong Bo Park, Ji Yeon Ahn, Woo Sub Yang, Seong Chae Park, Young Jin Jung, Myungook Lee, Byung Hee Hong, Jeong Mook Lim
Summary: This study investigated the effect of nanoscale spacing by stacked graphene film on osteogenic differentiation of human adipose-derived mesenchymal stromal cells. The results showed that osteogenic differentiation was promoted as the stacking layer increased, with the most significant effects observed in the three layers. The study demonstrated that multilayer-stacked graphene created nanoscale spaces promote calcium deposit and cytoskeletal integrity in hADSC-related, in vitro-osteogenesis.
Article
Biochemistry & Molecular Biology
Rokas Miksiunas, Ruta Aldonyte, Agne Vailionyte, Tadas Jelinskas, Romuald Eimont, Gintare Stankeviciene, Vytautas Cepla, Ramunas Valiokas, Kestutis Rucinskas, Vilius Janusauskas, Siegfried Labeit, Daiva Bironaite
Summary: Dilated cardiomyopathy (DCM) is a common type of heart disease characterized by ventricular dilation and impaired contraction. Research has shown that human mesenchymal stem/stromal cells (hmMSCs) from DCM patients can be induced to differentiate towards cardiac muscle direction with the use of HDAC inhibitor SAHA, potentially offering therapeutic benefits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Ejaz R. Dar, Mudasir B. Gugjoo, Moien Javaid, Shahid Hussain, Mujeeb R. Fazili, Kuldeep Dhama, Taha Alqahtani, Ali M. Alqahtani, Riaz A. Shah, Talha Bin Emran
Summary: The study evaluates the influence of ambient temperature, donor physiological status, and tissue source on sheep mesenchymal stem cells. Results show that adipose tissue-derived MSCs from full term pregnant female sheep exhibit higher potential for proliferation and differentiation compared to male sheep-derived MSCs. Cryopreserved MSCs have similar characteristics to fresh cells. Therefore, it is important to consider tissue type and donor physiological status when applying MSCs in clinical settings.
Article
Environmental Sciences
Barsha Roy, Hemamalini Chandrasekaran, Suresh Palamadai Krishnan, Natarajan Chandrasekaran, Amitava Mukherjee
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2018)
Article
Education, Scientific Disciplines
Suresh Palamadai Krishnan, Kaustubh Sunil Hiray, Siddhant Vyas
INDIAN JOURNAL OF PHARMACEUTICAL EDUCATION AND RESEARCH
(2018)
Article
Spectroscopy
P. K. Suresh, Divya Naik, Nidhi Shah, R. Rajasekaran
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2018)
Article
Biochemistry & Molecular Biology
Abhishek Sinha, P. K. Suresh
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2019)
Article
Biochemical Research Methods
Karthiga Durairaj, Barsha Roy, Natarajan Chandrasekaran, Suresh Palamadai Krishnan, Amitava Mukherjee
IET NANOBIOTECHNOLOGY
(2020)
Article
Education, Scientific Disciplines
Kaustubh Sunil Hiray, Suresh Palamadai Krishnan
INDIAN JOURNAL OF PHARMACEUTICAL EDUCATION AND RESEARCH
(2020)
Article
Engineering, Environmental
Barsha Roy, Khushboo Kadam, Suresh Palamadai Krishnan, Chandrasekaran Natarajan, Amitava Mukherjee
Summary: The study found that the binary mixture of titanium dioxide nanoparticles and tetracycline significantly increased cytotoxicity in plants, especially under UVA pre-irradiation. It also showed that both compounds had genotoxic potential in Allium cepa, leading to different chromosomal abnormalities.
FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING
(2021)
Article
Environmental Sciences
Barsha Roy, P. K. Suresh, Natarajan Chandrasekaran, Amitava Mukherjee
Summary: The study showed that the toxicity of a binary mixture of tetracycline and TiO2 nanoparticles on freshwater algae increased significantly, especially when used in combination with UV pre-irradiated nanoparticles.
Article
Engineering, Environmental
Barsha Roy, P. K. Suresh, Natarajan Chandrasekaran, Amitava Mukherjee
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2020)
Article
Education, Scientific Disciplines
Vembuli A. Varadharaj, P. K. Suresh
INDIAN JOURNAL OF PHARMACEUTICAL EDUCATION AND RESEARCH
(2020)
Review
Cell Biology
Suresh Palamadai Krishnan
