Article
Food Science & Technology
Anna Katharina Atanasoff-Kardjalieff, Lena Studt
Summary: This review summarizes the latest advances in secondary metabolite (SM) research in the economically important plant pathogen Fusarium, with a focus on chromatin structure.
Article
Biochemistry & Molecular Biology
Amandine Barral, Gabrielle Pozo, Lucas Ducrot, Giorgio L. Papadopoulos, Sandrine Sauzet, Andrew J. Oldfield, Giacomo Cavalli, Jerome Dejardin
Summary: A study revealed the presence of dual regions with both H3K9me3 and H3K36me3 marks in mouse embryonic stem cells, which lose these marks and gain enhancer signatures upon removal of SETDB1, suggesting an important role for heterochromatin in gene control. In differentiated tissues, some of these dual domains become destabilized and enriched in enhancer marks, providing insight into heterochromatin involvement in cell identity maintenance.
Article
Multidisciplinary Sciences
Haipeng Guan, Pei Wang, Pei Zhang, Chun Ruan, Yutian Ou, Bo Peng, Xiangdong Zheng, Jianlin Lei, Bing Li, Chuangye Yan, Haitao Li
Summary: The Rpd3S complex recognizes and deacetylates histones H3 and H4 at transcribed regions, guided by H3K36me3 modifications. Cryo-electron microscopy structures of Rpd3S in free and H3K36me3 nucleosome-bound states reveal a unique architecture. Multivalent recognition of H3K36me3 marks, nucleosomal DNA, and linker DNAs positions the catalytic center for deacetylation. Combinatorial readout of unmethylated H3K4 and H3K36me3 directs specific histone H3 deacetylation.
Article
Genetics & Heredity
Simone Bachleitner, Michael Sulyok, Jens Laurids Sorensen, Joseph Strauss, Lena Studt
Summary: Fusarium fujikuroi and Fusarium graminearum are important plant pathogens infecting staple food plants, with chromatin regulation playing a crucial role in their growth, virulence, and secondary metabolism.
FUNGAL GENETICS AND BIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Hao-Nan Wang, Xia Ke, Jun-Ping Zhou, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This article reviews the latest advances in the molecular regulation of GA(3) biosynthesis in F. fujikuroi and presents a comprehensive network involving nitrogen depression, global regulators, histone modification, and G protein signaling pathways. Furthermore, the article proposes bioengineering strategies, such as conventional random mutation, genetic manipulating platform development, metabolic editing, and fermentation optimization.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Na Li, Hong Yang, Ke Liu, Liwei Zhou, Yuting Huang, Danyan Cao, Yanlian Li, Yaoliang Sun, Aisong Yu, Zhiyan Du, Feng Yu, Ying Zhang, Bingyang Wang, Meiyu Geng, Jian Li, Bing Xiong, Shilin Xu, Xun Huang, Tongchao Liu
Summary: Researchers have developed a potent inhibitor 38 that selectively targets the NSD2-PWWP1 domain. This inhibitor has been shown to affect the expression of genes regulated by NSD2.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Yu Liu, Yang Sun, Yang Bai, Xin Cheng, Hui Li, Xing Chen, Yu Chen
Summary: This study investigated the resistance of the rice bakanae disease pathogen, F. fujikuroi, to the novel succinate dehydrogenase inhibitor Pydiflumetofen (Pyd). Through the generation of resistant mutants, different levels of resistance were identified, along with specific genotypes associated with resistance. The study suggests that the risk of resistance to Pyd in F. fujikuroi is moderate and comes with significant fitness cost.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Giha Song, Seungmin Son, Suhyeon Nam, Eun-Jung Suh, Soo In Lee, Sang Ryeol Park
Summary: Invasive pathogens cause significant damage to crops annually, and identifying genes that confer broad-spectrum resistance is crucial for plant breeding. This study reveals that OsWRKY114 enhances innate immunity in rice against the fungal pathogen Fusarium fujikuroi, in addition to its previously known role against the bacterial pathovar Xanthomonas oryzae pv. oryzae. Overexpression of OsWRKY114 in rice reduces the expression of susceptibility-related genes and increases resistance against F. fujikuroi.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Ling Hao, Mengjuan Zhang, Chi Yang, Xiaohua Pan, Dandan Wu, Hong Lin, Dongmei Ma, Yanfang Yao, Wangzhuo Fu, Jiarui Chang, Yanling Yang, Zhenhong Zhuang
Summary: This study revealed the crucial role of a protein called Set9 in the growth, reproduction, and mycotoxin production of Aspergillus flavus. It was found that Set9 regulates various physiological responses and metabolic production of the fungus through catalyzing specific histone modifications, while also affecting its colonization ability on crop kernels by adjusting sensitivity to oxidation stress and cell wall integrity stress. Furthermore, this study identified the key amino acid in the active center of Set9 and discovered its regulation of the aflatoxin gene cluster through a different protein.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Yixiu Song, Xing Chen, Jiazhi Sun, Yang Bai, Ling Jin, Yujie Lin, Yang Sun, Haiqun Cao, Yu Chen
Summary: In this study, the baseline sensitivity of Fusarium fujikuroi to azoxystrobin was established by testing 100 isolates from Anhui Province of China. Azoxystrobin exhibited strong activity against the isolates, with no cross-resistance observed for other fungicides. Furthermore, a mutation in cyt b gene (G143A) was found to confer resistance to azoxystrobin.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Microbiology
Hubiao Jiang, Na Wu, Shaomin Jin, Temoor Ahmed, Hui Wang, Bin Li, Xiaobi Wu, Yidan Bao, Fei Liu, Jing-Ze Zhang
Summary: This study isolated 421 strains of Fusarium species from 25 rice samples collected from three provinces in China, with the majority identified as F. fujikuroi. Phylogenetic analysis revealed six different species, and pathogenicity tests showed varying abilities in causing bakanae disease in rice seedlings. Seed germination assays demonstrated different effects of certain isolates on seed germination and seed and bud rot. Additionally, a LAMP-based assay and a species-specific marker were developed for efficient and sensitive detection of F. fujikuroi.
Article
Biochemistry & Molecular Biology
Xuheng Gao, Qin Peng, Kang Yuan, Yun Li, Mengru Shi, Jianqiang Miao, Xili Liu
Summary: Rice bakanae disease caused by Fusarium fujikuroi is a destructive seed-borne disease worldwide. Prochloraz resistance in China is severe, with reduced fitness in resistant populations. There is no cross-resistance between prochloraz and tebuconazole or hexaconazole, and resistant isolates remain sensitive to fludioxonil, phenamacril, and pydiflumetofen. Highly resistant isolates show S312T mutation in Ffcyp51b or overexpression of Ffcyp51a and Ffcyp51b. Attention should be paid to the genotype carrying S312T mutation when monitoring and managing prochloraz resistance in F. fujikuroi.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Plant Sciences
Fang Jing Li, Ryoji Komura, Chiharu Nakashima, Masafumi Shimizu, Koji Kageyama, Haruhisa Suga
Summary: Fusarium fujikuroi, the pathogen of rice bakanae disease, can be subclassified into two groups: gibberellin (G) and fumonisin (F). Thiophanate-methyl (TM), a benzimidazole fungicide, has been widely used to control F. fujikuroi. In Japan, it was found that F-group strains are TM sensitive (TMS), while most G-group strains are TM resistant (TMR). The TMR strains have mutations E198K and F200Y in beta(2)-tubulin. A diagnostic method using loop-mediated isothermal amplification with fluorescent loop primers was developed and applied to identify these mutations in 37 TMR strains and 56 TMS strains. The results showed that 100% of the TMR strains had either the E198K mutation (41%) or the F200Y mutation (59%), while none of the TMS strains had these mutations. Interestingly, a TMR strain in the F group with an F200Y mutation was discovered. These findings suggest that the E198K and F200Y mutations in beta(2)-tubulin contribute to TM resistance in F. fujikuroi.
Article
Biochemistry & Molecular Biology
Svetlana Sharifulina, Valentina Dzreyan, Valeria Guzenko, Svetlana Demyanenko
Summary: The study found that the levels and intracellular localization of DNMT1 and G9a proteins changed in penumbra neurons and astrocytes in the rat cerebral cortex after stroke. Inhibition of DNMT1 and G9a protected penumbra cells from apoptosis and reduced the volume of cerebral infarction induced by stroke.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Qin Peng, Muhammad Waqas Younas, Jikun Yang, Hongwei Zhu, Jianqiang Miao, Biao Gu, Xili Liu
Summary: This study investigated the development of Prochloraz resistance in Fusarium fujikuroi and analyzed the fitness of different resistant strains. The majority of the F. fujikuroi strains collected from Heilongjiang Province showed resistance to Prochloraz, with a resistance frequency of 92.1%. The resistant strains demonstrated higher disease severity and there was no cross-resistance between Prochloraz and other tested fungicides. These results indicate that Prochloraz resistance in F. fujikuroi is severe and could potentially lead to yield loss.
Article
Cardiac & Cardiovascular Systems
Ling Li, Shichao Pang, Lingyao Zeng, Ulrich Gueldener, Heribert Schunkert
Summary: Genetic components of intelligence affect measured intelligence and educational attainment, which subsequently influence the prevalence of coronary artery disease through a series of unfavorable risk factor profiles.
CLINICAL RESEARCH IN CARDIOLOGY
(2021)
Article
Plant Sciences
Michael Mentges, Anika Glasenapp, Marike Boenisch, Sascha Malz, Bernard Henrissat, Rasmus J. N. Frandsen, Ulrich Gueldener, Martin Muensterkoetter, Joerg Bormann, Marc-Henri Lebrun, Wilhelm Schaefer, Ana Lilia Martinez-Rocha
MOLECULAR PLANT PATHOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Maria J. Tavares, Ulrich Gueldener, Ana Mendes-Ferreira, Nuno P. Mira
Summary: Resequencing of S. ludwigii UTAD17 genome using PacBio resulted in 20 contigs totaling 13 Mb of assembled DNA, representing 95% of the DNA content. Annotation of the assembled genome predicted 4644 protein-encoding genes, including 213 proteins unique to this species.
Article
Biotechnology & Applied Microbiology
Leonie Baumann, Tyler Doughty, Verena Siewers, Jens Nielsen, Eckhard Boles, Mislav Oreb
Summary: In-depth study of cell physiology during octanoic acid production in Saccharomyces cerevisiae revealed increased production levels of octanoic acid and other chain length fatty acids, alongside decreased growth rate in the production strain. Transcriptomic response to internally produced octanoic acid was notably different from response to externally supplied octanoic acid, with identification of a gene, RPL40B, previously unknown to be involved in fatty acid biosynthesis or medium-chain fatty acid tolerance. Overexpression of RPL40B led to a 40% increase in octanoic acid titers.
FEMS YEAST RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Florian Wernig, Leonie Baumann, Eckhard Boles, Mislav Oreb
Summary: Redirecting glucose flux towards the oxidative branch of the pentose phosphate pathway and overexpressing a heterologous phosphoketolase/phosphotransacetylase shunt improved OA yield by over 60% compared to the parental strain. Toxicity assays suggest that the inhibitory effects of OA on cell growth may impose an upper limit on achievable OA yields. Further investigation of genetic engineering targets is needed to overcome limitations in OA production in yeast.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemical Research Methods
Leonie Baumann, Stefan Bruder, Johannes Kabisch, Eckhard Boles, Mislav Oreb
Summary: The study focuses on microbial biosynthesis of octanoic acid through yeast, utilizing a biosensor for high-throughput screening of new genetic targets and successfully identifying KCS1 and FSH2 as genes that can increase octanoic acid titers.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Food Science & Technology
Asja Ceranic, Thomas Svoboda, Franz Berthiller, Michael Sulyok, Jonathan Matthew Samson, Ulrich Gueldener, Rainer Schuhmacher, Gerhard Adam
Summary: This study identified and characterized the genes encoding fusaproliferin biosynthetic enzymes in Fusarium proliferatum, showing the significant roles of specific enzymes for fusaproliferin production. The knock-out mutants further confirmed the functions of these enzymes and provided insights into the biosynthesis of fusaproliferin and its novel derivatives.
Article
Biochemistry & Molecular Biology
Alexander U. Bissell, Julia Rautschek, Sandra Hoefgen, Luka Raguz, Derek J. Mattern, Nauman Saeed, Slavica Janevska, Katarina Jojic, Ying Huang, Johann E. Kufs, Barbara Herboeck, Huijuan Guo, Falk Hillmann, Christine Beemelmanns, Vito Valiante
Summary: This study identified and elucidated the biosynthetic gene cluster responsible for the biosynthesis of sphingofungins B, C, and D in Aspergillus fumigatus, as well as the initial steps of sphingofungin biosynthesis. Furthermore, different aminomalonate derivatives were found to be used as substrates for various chemical variants of sphingofungins produced by Paecilomyces variotii, enhancing our understanding of the general biosynthesis of sphingolipid inhibitors in fungi.
ACS CHEMICAL BIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Ling Li, Zhifen Chen, Moritz von Scheidt, Shuangyue Li, Andrea Steiner, Ulrich Gueldener, Simon Koplev, Angela Ma, Ke Hao, Calvin Pan, Aldons J. Lusis, Shichao Pang, Thorsten Kessler, Raili Ermel, Katyayani Sukhavasi, Arno Ruusalepp, Julien Gagneur, Jeanette Erdmann, Jason C. Kovacic, Johan L. M. Bjorkegren, Heribert Schunkert
Summary: This study systematically identified susceptibility genes for coronary artery disease (CAD) using transcriptome-wide association studies (TWAS). The study prioritized candidate causal genes at known GWAS loci, identified 18 novel genes associated with CAD, and suggested potential tissues and pathways of action for these TWAS CAD genes.
BASIC RESEARCH IN CARDIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Chuhua Yang, Fabian Starnecker, Shichao Pang, Zhifen Chen, Ulrich Guldener, Ling Li, Matthias Heinig, Heribert Schunkert
Summary: While the prevalence of CAD is higher in Scotland compared to England, traditional risk factors and genetic risk scores based on common alleles do not fully explain this difference. Differences in the allele frequencies of individual risk variants suggest that geographically and ethnically related populations may still show relevant differences in the genetic architecture of a common disease.
BMC CARDIOVASCULAR DISORDERS
(2021)
Article
Microbiology
Paul J. McMurdie, Magdalena K. Stoeva, Nicholas Justice, Madeleine Nemchek, Christian M. K. Sieber, Surabhi Tyagi, Jessica Gines, Connor T. Skennerton, Michael Souza, Orville Kolterman, John Eid
Summary: This study demonstrates an increase in circulating butyrate and ursodeoxycholate following probiotic intervention in humans with T2D, suggesting a potential targeted microbiome-based approach for managing T2D. The efficient synthesis of UDCA by C. butyricum may also be of interest for probiotic use in other disease settings. Additionally, the inhibitory interaction between sulfonylurea drugs and gut microbiota should be carefully considered in future study designs.
Article
Biochemistry & Molecular Biology
Katrina D. Neiburga, Baiba Vilne, Sabine Bauer, Dario Bongiovanni, Tilman Ziegler, Mark Lachmann, Simon Wengert, Johann S. Hawe, Ulrich Gueldener, Annie M. Westerlund, Ling Li, Shichao Pang, Chuhua Yang, Kathrin Saar, Norbert Huebner, Lars Maegdefessel, Ruediger Lange, Markus Krane, Heribert Schunkert, Moritz von Scheidt
Summary: This study analyzed tissue-specific miRs derived from IMA samples from 192 CAD patients and identified 17 miRs significantly correlated with cardiovascular risk factors. Of particular interest, miR-5701 was positively correlated with hypertension, hypercholesterolemia, and diabetes, while miR-629-5p and miR-98-5p were significantly correlated with acute myocardial infarction. This first atlas of miR profiles in IMA samples from CAD patients may play an important role in improved risk assessment, mechanistic disease understanding, and local therapy of CAD.
Article
Genetics & Heredity
Krishna G. Aragam, Tao Jiang, Anuj Goel, Stavroula Kanoni, Brooke N. Wolford, Deepak S. Atri, Elle M. Weeks, Minxian Wang, George Hindy, Wei Zhou, Christopher Grace, Carolina Roselli, Nicholas A. Marston, Frederick K. Kamanu, Ida Surakka, Loreto Munoz Venegas, Paul Sherliker, Satoshi Koyama, Kazuyoshi Ishigaki, Bjorn O. Asvold, Michael R. Brown, Ben Brumpton, Paul S. de Vries, Olga Giannakopoulou, Panagiota Giardoglou, Daniel F. Gudbjartsson, Ulrich Gueldener, Syed M. Ijlal Haider, Anna Helgadottir, Maysson Ibrahim, Adnan Kastrati, Thorsten Kessler, Theodosios Kyriakou, Tomasz Konopka, Ling Li, Lijiang Ma, Thomas Meitinger, Soeren Mucha, Matthias Munz, Federico Murgia, Jonas B. Nielsen, Markus M. Noethen, Shichao Pang, Tobias Reinberger, Gavin Schnitzler, Damian Smedley, Gudmar Thorleifsson, Moritz von Scheidt, Jacob C. Ulirsch, David O. Arnar, Noel P. Burtt, Maria C. Costanzo, Jason Flannick, Kaoru Ito, Dong-Keun Jang, Yoichiro Kamatani, Amit V. Khera, Issei Komuro, Iftikhar J. Kullo, Luca A. Lotta, Christopher P. Nelson, Robert Roberts, Gudmundur Thorgeirsson, Unnur Thorsteinsdottir, Thomas R. Webb, Aris Baras, Johan L. M. Bjoerkegren, Eric Boerwinkle, George Dedoussis, Hilma Holm, Kristian Hveem, Olle Melander, Alanna C. Morrison, Marju Orho-Melander, Loukianos S. Rallidis, Arno Ruusalepp, Marc S. Sabatine, Kari Stefansson, Pierre Zalloua, Patrick T. Ellinor, Martin Farrall, John Danesh, Christian T. Ruff, Hilary K. Finucane, Jemma C. Hopewell, Robert Clarke, Rajat M. Gupta, Jeanette Erdmann, Nilesh J. Samani, Heribert Schunkert, Hugh Watkins, Cristen J. Willer, Panos Deloukas, Sekar Kathiresan, Adam S. Butterworth
Summary: This study identified and characterized over 250 risk loci for coronary artery disease (CAD) through a genome-wide association study, shedding light on the potential causal mechanisms for CAD. The research findings revealed the involvement of early developmental processes, cell cycle signaling, and vascular cell migration and proliferation in the pathogenesis of CAD.
Article
Medicine, General & Internal
Ulrich Gueldener, Thorsten Kessler, Moritz von Scheidt, Johann S. Hawe, Beatrix Gerhard, Dieter Maier, Mark Lachmann, Karl-Ludwig Laugwitz, Salvatore Cassese, Albert W. Schoemig, Adnan Kastrati, Heribert Schunkert
Summary: This study applied self-organizing maps (SOMs) to detect regular patterns in multi-layered data for better prediction of in-stent restenosis (ISR) after percutaneous coronary intervention with stenting. The SOM approach identified known and novel predictors for restenosis risk, but did not significantly improve the prediction of ISR compared to conventional multivariable models.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Computer Science, Artificial Intelligence
Johannes Krefting, Partho Sen, Diana David-Rus, Ulrich Gueldener, Johann S. Hawe, Salvatore Cassese, Moritz von Scheidt, Heribert Schunkert
Summary: Outcome research supporting guideline recommendations for primary and secondary preventions relies heavily on data from clinical trials and selected hospital populations. The increasing volume of real-world medical data could significantly enhance the prediction, prevention, and care of cardiovascular disease (CVD). This review highlights the potential of health insurance claims (HIC) data to improve healthcare provision and discusses the challenges faced by patients, physicians, health insurers, and policy makers in utilizing such data.
FRONTIERS IN ARTIFICIAL INTELLIGENCE
(2023)