期刊
FRONTIERS IN PLANT SCIENCE
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2017.00006
关键词
Nelumbo nucifera; small RNA; microRNAs; submergence; high-throughput sequencing
资金
- National Natural Science Foundation of China [31501795, 31400600]
- Fundamental Research Funds for the Central Universities [KJQN201659]
- China Postdoctoral Science Foundation [2014M560432, 2015T80563]
- agricultural science and technology innovation of Jiangsu Province, China [CX(15)1030, CX(16)1024]
- Natural Science Foundation of Jiangsu Province in China [BK20151229, BK20140695]
MicroRNAs (miRNAs) are endogenous non-coding RNAs with important regulatory functions in plant development and stress responses. However, their population abundance in lotus (Nelumbo nucifera Gaertn) has so far been poorly described, particularly in response to stresses. In this work, submergence-related miRNAs and their target genes were systematically identified, compared, and validated at the transcriptome-wide level using high-throughput sequencing data of small RNA, Mrna, and the degradome. A total of 128 known and 20 novel miRNAs were differentially expressed upon submergence. We identified 629 target transcripts for these submergence-responsive miRNAs. Based on the miRNA expression profiles and GO and KEGG annotation of miRNA target genes, we suggest possible molecular responses and physiological changes of lotus in response to submergence. Several metabolic, physiological and morphological adaptations-related miRNAs, i.e., NNU_far-miR159, NNU_gma-miR393h, and NNU_aly-miR319c-3p, were found to play important regulatory roles in lotus response to submergence. This work will contribute to a better understanding of miRNA-regulated adaption responses of lotus to submergence stress.
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