Review
Biology
Ricardo Mir, Antonio Calabuig-Serna, Jose M. Segui-Simarro
Summary: This review compiles the latest advances in doubled haploid plant production, the application of doubled haploid lines in breeding programs, and future perspectives for alternative technologies for doubled haploid generation in eggplant. Eggplant is globally cultivated for its edible fruit, and breeding programs aim to produce F1 hybrids by crossing highly homozygous pure lines. Doubled haploid individuals can be induced from haploid cells in a single generation, offering a more efficient alternative to traditional self crossing methods.
Article
Plant Sciences
Antonio Calabuig-Serna, Carolina Camacho-Fernandez, Ricardo Mir, Rosa Porcel, Esther Carrera, Isabel Lopez-Diaz, Jose M. Segui-Simarro
Summary: During eggplant microspore embryogenesis, the transition from embryos to calli instead of developing as true embryos is a bottleneck. Studies show that there are no genotype-specific endogenous GR profiles associated with a high embryogenic response. The role of GRs in the development of microspore-derived embryos in eggplant cultures is highlighted, with different GR accumulation patterns influencing the embryogenic response.
PLANT GROWTH REGULATION
(2022)
Article
Plant Sciences
Andrea D. Koehler, Monica L. Rossi, Vera T. C. Carneiro, Glaucia B. Cabral, Adriana P. Martinelli, Diva M. A. Dusi
Summary: Brachiaria, a widely cultivated forage plant species in Brazil, has significant agronomical importance. Within the species, the apomictic tetraploid cultivar B. brizantha cv. Marandu (syn. Urochloa brizantha) is particularly valued due to its extensive cultivation. However, the difference in ploidy levels between sexual and apomictic plants hinders breeding programs for Brachiaria. The induction of haploids and double haploids is therefore of great interest for generating novel genotypes for intraspecific crosses.
Review
Plant Sciences
Brett Hale, Alison M. R. Ferrie, Sreekala Chellamma, J. Pon Samuel, Gregory C. Phillips
Summary: Androgenesis is widely used in plant breeding programs to redirect cell fate and study cell cycle regulation, meiotic recombination, and embryogenesis. The focus has shifted from protocol development to understanding how protocols work in order to extend their application to other genotypes and species. Transcriptomic and epigenetic analyses have revealed cellular and molecular responses associated with androgenesis, and gene transfer and genome editing technologies show promise for enhancing the genetic composition of microspores used in breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Samira Tajedini, Baratali Fakheri, Mohsen Niazian, Nafiseh Mahdinezhad, Abdelbagi Mukhtar Ali Ghanim, Ali Kazemi Pour, Ivan Ingelbrecht, Mehran E. Shariatpanahi
Summary: Two different androgenesis pathways, shed-microspore culture (SMC) and anther culture (AC), were developed for haploid induction in mutagenized M-1 rice cultivars. The effects of various factors on induction and regeneration phases were evaluated. The SMC method showed better results in terms of the number of regenerated embryos. Chromosome counting and flow cytometry analysis confirmed the presence of haploid plants, providing a valuable foundation for rice breeding research.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Review
Agronomy
Agnieszka Kielkowska, Waldemar Kiszczak
Summary: This paper presents the historical background and current status of haploid induction in carrot, and discusses the potential of haploidization technology in carrot breeding. The methods of androgenesis, gynogenesis, and induced parthenogenesis, as well as the manipulation of the centromere-specific histone 3 variant (CENH3) in carrot, are also acknowledged.
Article
Plant Sciences
Monika Gajecka, Marek Marzec, Beata Chmielewska, Janusz Jelonek, Justyna Zbieszczyk, Iwona Szarejko
Summary: This study investigated the genotype-dependent molecular differences in chloroplast differentiation leading to the formation of green and albino regenerants in microspore culture of barley. The results showed that the failed transition from NEP-dependent to PEP-dependent transcription in plastids was associated with albino regenerants, while a high activity of PEP polymerase and increased expression of certain genes correlated with the production of green regenerants. Understanding these molecular mechanisms may help overcome the issue of albinism in cereal androgenesis.
Article
Plant Sciences
Jorge Lora, Andres Garcia-Lor, Pablo Aleza
Summary: Seedlessness is a crucial agronomic trait in mandarins, and creating triploid plants is an important strategy for developing new seedless citrus varieties. However, little is known about the key steps of pollen development in polyploids. A study was conducted to investigate the effect of ploidy level on pollen development in diploid and tetraploid genotypes. The findings revealed significant differences in cell area, shape, starch hydrolysis, and carbohydrate accumulation during microgametogenesis, which resulted in the infertility of pollen grains in tetraploid Clemenules clementine. This poses challenges in using tetraploid genotypes as male parents for hybridization in order to produce large progenies of triploid hybrids.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Alberto Galan-Avila, Edgar Garcia-Fortea, Jaime Prohens, Francisco Javier Herraiz
Summary: In Cannabis sativa, the development of double haploids is a challenging and important research topic, involving the entire process of anther and pollen grain formation, microsporogenesis, and microgametogenesis. Furthermore, cold pretreatment can alter the gametophytic pathway, inducing embryogenic development in male flowers.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Vineeta Shrivastava, Arockiasamy Savarimuthu, Manoj Patil, Purbasha Sarkar, Sainath Hadole, Santanu Dasgupta
Summary: Development of haploid and doubled haploids in Jatropha microspore culture was achieved through the establishment of an efficient isolated microspore culture system and optimization of various factors affecting microspore embryogenesis. Microscopic and flow cytometric analyses confirmed the success of gametic embryogenesis in Jatropha microspore culture.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Multidisciplinary Sciences
Maria de los Angeles Bohorquez-Quintero, Daicy Yaneth Galvis-Tarazona, Diana Marcela Arias-Moreno, Zaida Zarely Ojeda-Perez, Sergio Ochatt, Luis Ernesto Rodriguez-Molano
Summary: This study reports for the first time the characterization of anther development and pollen formation in the yellow potato cultivar Criolla Colombia, providing valuable information for understanding the genetic resource of diploid potatoes. The study also proposes a guide for efficient obtaining of haploids by determining the optimal bud stage for haploid production.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Brett Hale, Callie Phipps, Naina Rao, Caroline Kelley, Gregory C. Phillips
Summary: The study successfully diverted isolated soybean microspores from a gametophytic pathway through temperature stress, as indicated by transcriptomic analysis. Cytological characteristics observed in isolated microspore culture supported previous claims of cell fate redirection in soybean microspores. This research provides insights into gametophytic embryogenesis induction and may be useful for soybean androgenesis investigation.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2021)
Article
Plant Sciences
Dong-Rui Kong, Melanie Schori, Lu Li, Yan Luo, De-Chang Hu
Summary: This study investigates the anther and ovule developmental characters of Pittosporopsis and compares them with other members of Metteniusaceae and Icacinaceae. The study provides new insights into the morphological synapomorphies of Metteniusaceae and the embryology of early diverging campanulid clades.
Article
Agronomy
Ade Mulyana, Bambang S. Purwoko, Iswari S. Dewi, Awang Maharijaya
Summary: Eggplant is a highly produced and valuable vegetable crop. Anther culture is used to produce pure lines for hybrid breeding. This study compared six anther culture methods and found that the Modification 1 method was the most effective in producing in vitro androgenic regenerated plants in eggplant. The ploidy level of regenerants was confirmed using flow cytometry.
Article
Plant Sciences
Mariana Ferreira Alves, Fabio Pinheiro, Karen Lucia Gama De Toni, Jose Fernando Andrade Baumgratz
Summary: The study on Epidendrum species belonging to the subgenus Amphyglottium revealed that their pollinia and caudicle have a stable characteristic, regardless of their different pollination strategies and habitats. The male gametophyte formation process shows no interspecific variation among these species, indicating a stable trait for the genus.
BRAZILIAN JOURNAL OF BOTANY
(2023)
Article
Biotechnology & Applied Microbiology
Sara Sohrabi, Mohammad Reza Abdollahi, Asghar Mirzaie-Asl, Hassan Ebrahimi Koulaei, Mohsen Aghaeezadeh, Jose M. Segui-Simarro
Summary: The study found that pretreatments with 0.5 M mannitol and 50μM 5-AzaC, as well as the use of MS basal medium and 60 g/L sucrose, notably improved the efficiency of haploid induction and plant regeneration through ovule culture in sugar beet. This new method could potentially be applicable to other crops as well.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Biotechnology & Applied Microbiology
Esin Ari, Hilal Bedir, Ismail Gokhan Deniz, Ilker Genc, Jose M. Segui-Simarro
Summary: The study focused on exploring the androgenic potential of different wild Turkish V. hispanica genotypes as a basis for identifying bottlenecks in future studies. While almost all genotypes responded to microspore embryogenesis, three main bottlenecks were identified: foam-producing saponins in flowers hindered efficient isolation of microspores, embryos had reduced germination ability, and a dense network of hairy roots prevented the formation of functional root systems.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Review
Biology
Ricardo Mir, Antonio Calabuig-Serna, Jose M. Segui-Simarro
Summary: This review compiles the latest advances in doubled haploid plant production, the application of doubled haploid lines in breeding programs, and future perspectives for alternative technologies for doubled haploid generation in eggplant. Eggplant is globally cultivated for its edible fruit, and breeding programs aim to produce F1 hybrids by crossing highly homozygous pure lines. Doubled haploid individuals can be induced from haploid cells in a single generation, offering a more efficient alternative to traditional self crossing methods.
Article
Plant Sciences
Antonio Calabuig-Serna, Carolina Camacho-Fernandez, Ricardo Mir, Rosa Porcel, Esther Carrera, Isabel Lopez-Diaz, Jose M. Segui-Simarro
Summary: During eggplant microspore embryogenesis, the transition from embryos to calli instead of developing as true embryos is a bottleneck. Studies show that there are no genotype-specific endogenous GR profiles associated with a high embryogenic response. The role of GRs in the development of microspore-derived embryos in eggplant cultures is highlighted, with different GR accumulation patterns influencing the embryogenic response.
PLANT GROWTH REGULATION
(2022)
Article
Plant Sciences
Carolina Camacho-Fernandez, Jose M. Segui-Simarro, Ricardo Mir, Kim Boutilier, Patricia Corral-Martinez
Summary: Different types of embryogenic structures exhibit specific cell wall characteristics that determine their developmental fate. Highly embryogenic structures have thin walls rich in specific components, while less embryogenic structures have thick walls and lower values of cell wall components.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Hilal Bedir, Esin Ari, Gulsun Elif Vural, Jose M. Segui-Simarro
Summary: Vaccaria hispanica, an interesting species with attractive agronomic properties and valuable bioactive compounds, has potential applications in various fields. However, there is a lack of studies on in vitro clonal population production. This study investigates the possibilities of regenerating clonal plants from two wild Turkish genotypes through somatic embryogenesis and organogenesis, and identifies suitable explant types and plant growth regulator combinations for inducing different morphogenic processes.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Plant Sciences
Antonio Calabuig-Serna, Ricardo Mir, Paloma Arjona, Jose Maria Segui-Simarro
Summary: In this study, the role of calcium in somatic embryogenesis in carrot was investigated. It was found that high calcium levels and the formation of callose layer are indicators of embryogenesis induction. Ca2+ patterns vary in different embryogenic structures. Modulating intracellular Ca2+ levels can effectively regulate the efficiency of somatic embryogenesis in carrot. Additionally, Ca2+-calmodulin signaling is associated with transcriptional remodeling necessary for embryo progression.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Antonio Calabuig-Serna, Ricardo Mir, Jose M. Segui-Simarro
Summary: In this study, somatic embryogenesis in Arabidopsis was induced using IZEs as explants. The process was characterized at the microscopic level, and specific aspects such as WUS expression, callose deposition, and Ca2+ dynamics were studied. The results showed that somatic embryos are produced from WUS-expressing cells in a finger-like appendix, and Ca2+ levels and callose deposition serve as early markers of the embryogenic regions. The study also found that Ca2+ homeostasis in this system is strictly maintained and cannot be altered to modulate embryo production.
Article
Plant Sciences
Antonio Calabuig-Serna, Ricardo Mir, Rosa Porcel, Jose M. Segui-Simarro
Summary: Brassica napus is a species of high agronomic interest commonly used for studying various processes, including microspore embryogenesis. DH4079 and DH12075 lines, with high and low embryogenic response respectively, are ideal for investigating the mechanisms controlling embryogenesis induction. This study examined the genetic transformation ability of DH4079 plants and compared it with the already studied DH12075 plants. Results showed that DH12075 plants responded to genetic transformation, while DH4079 plants remained recalcitrant, failing to produce any regenerant. The study also tested transient transformation with Agrobacterium rhizogenes, where only DH12075 showed a response. These findings indicate that DH4079 is recalcitrant to Agrobacterium-mediated transformation.
Article
Agronomy
Jose M. Segui-Simarro, Nathanael M. A. Jacquier, Thomas Widiez
Summary: Doubled haploids (DH) have become a powerful tool used by breeding companies to produce pure lines for hybrid seed production. The biotechnological revolution has led to the development of multiple approaches for producing haploids and DHs in various species, offering flexibility and reliability in the process.
DOUBLED HAPLOID TECHNOLOGY, VOL. 1: General Topics, Alliaceae, Cereals
(2021)
