Article
Microbiology
Jose Angel Moreno-Cabezuelo, Guadalupe Gomez-Baena, Jesus Diez, Jose Manuel Garcia-Fernandez
Summary: This study compared the effects of different concentrations of glucose on the proteome and metabolome of Synechococcus sp. and Prochlorococcus sp. strains. The results showed that these cyanobacteria metabolize glucose primarily through oxidative pentoses and Calvin pathways, and no evidence was found for the involvement of the Entner-Doudoroff pathway. Differences in glucose effects were observed between genera and between Prochlorococcus MED4 and SS120 strains, possibly due to their specific adaptations to the environment. Additionally, fermentation was observed in Prochlorococcus sp. strain SS120 and Synechococcus sp. strain WH8102 after 5 mM glucose addition.
MICROBIOLOGY SPECTRUM
(2023)
Review
Microbiology
J. Diez, A. Lopez-Lozano, M. A. Dominguez-Martin, G. Gomez-Baena, M. C. Munoz-Marin, Y. Melero-Rubio, J. M. Garcia-Fernandez
Summary: Marine picocyanobacteria, such as Prochlorococcus and Synechococcus, have developed unique adaptive mechanisms in nitrogen metabolism to thrive in oligotrophic oceans, including strategies such as reducing GC and protein contents, utilizing truncated proteins, and perceiving nanomolar nitrate concentrations. These adaptations contrast with the knowledge obtained in freshwater cyanobacteria and are essential for the ecological success of these microorganisms.
FEMS MICROBIOLOGY REVIEWS
(2023)
Article
Plant Sciences
Chananwat Kortheerakul, Hakuto Kageyama, Rungaroon Waditee-Sirisattha
Summary: The study identified and characterized the GST gene family in the halotolerant cyanobacterium Halothece sp. PCC7418. One of the GSTs, H3557, showed significant upregulation under salt stress and exhibited GST activity towards CDNB and GSH with a broad range of enzymatic activity. This salt-adaptive enzyme remained catalytically active in the presence of NaCl and conferred tolerance to H2O2-induced oxidative stress and salt stress in heterologous expression systems.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Mara Simonazzi, Laura Pezzolesi, Paola Galletti, Chiara Gualandi, Rossella Pistocchi, Nicole De Marco, Zoe Paganelli, Chiara Samori
Summary: The study successfully optimized the production conditions for PHB by the cyanobacterium Anabaena sp., demonstrating efficient PHB production under specific conditions. After large-scale cultivation, PHB accounted for 46% of the biomass, with high quality attributes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Environmental Sciences
Neha Arora, George P. Philippidis
Summary: The study reveals that Chlorella vulgaris exhibits better nutrient stress tolerance and redox balancing under mixotrophic conditions, leading to higher biomass and lipid production.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemical Research Methods
Hannah De los Santos, Kristin P. Bennett, Jennifer M. Hurley
Summary: This study used the MOSAIC application, which combines model selection and joint modeling of multiple omics types, to successfully recover circadian rhythms in organisms, showing excellent performance both in the proteome and transcriptome. Additionally, this approach allows for the recognition of the diversity of circadian regulation compared to non-circadian regulation.
Article
Biotechnology & Applied Microbiology
Pragasit Itthirit, Aran Incharoensakdi, Tanakarn Monshupanee
Summary: The study optimized the single-stage cultivation of a photoheterotrophic cyanobacterium to evaluate the mass conversion efficiency of an organic substrate to both PHB and glycogen. Through adjustment of culture conditions, organic substrate supply, and cultivation time, the mass CE of glucose and acetate to these two biopolymers was higher than previous reports in photoheterotrophic microbes.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Multidisciplinary Sciences
Vanessa Krauspe, Matthias Fahrner, Philipp Spat, Claudia Steglich, Nicole Frankenberg-Dinkel, Boris Macek, Oliver Schilling, Wolfgang R. Hess
Summary: The protein NblD plays a crucial role in the degradation of phycobilisomes in cyanobacteria under nitrogen-limited conditions. Studies have shown that NblD is essential for maintaining normal growth of cyanobacterial cells during nitrogen limitation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Green & Sustainable Science & Technology
Nermin El Semary, Amira Mohamed Abd El-Sattar, Eman Zakaria Ahmed, Munirah Aldayel
Summary: The use of algae as biofertilizers is becoming widespread to meet the high demands for agricultural products. To achieve this, the nutritional components of algae must be optimized through mixotrophic conditions.
Article
Multidisciplinary Sciences
Juan Jose Pierella Karlusich, Eric Pelletier, Fabien Lombard, Madeline Carsique, Etienne Dvorak, Sebastien Colin, Marc Picheral, Francisco M. Cornejo-Castillo, Silvia G. Acinas, Rainer Pepperkok, Eric Karsenti, Colomban de Vargas, Patrick Wincker, Chris Bowler, Rachel A. Foster
Summary: This study provides an improved global overview of diazotroph abundance, diversity, and distribution by combining a quantitative image analysis pipeline with metagenomic mining using Tara Oceans datasets. Nitrogen fixation by diazotrophs is critical for marine primary production, with larger diazotrophs dominating tropical belts and unicellular cyanobacterial and non-cyanobacterial diazotrophs globally distributed in surface and mesopelagic layers.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Medicinal
Despoina Konstantinou, Rafael Popin, David P. Fewer, Kaarina Sivonen, Spyros Gkelis
Summary: Sponges form symbiotic relationships with diverse and abundant microbial communities, with cyanobacteria playing important roles. Genomic analysis of the cyanobacterial genus Leptothoe revealed sponge-associated strains showing features of symbiotic lifestyle despite genome reduction, harboring biosynthetic gene clusters for novel natural products. Comparisons with chemically rich cyanobacteria suggest Leptothoe as a promising candidate for biosynthesis of novel natural products in marine environments.
Article
Plant Sciences
Yumeng Zhang, Yaqi Wang, Wei Wei, Min Wang, Shuzhao Jia, Mingkun Yang, Feng Ge
Summary: Protein homeostasis in cyanobacteria is regulated by a proteolytic complex called ClpXP, which consists of ATPase ClpX and peptidase ClpP. This study aimed to investigate the functions and regulatory networks of ClpX in Synechocystis by identifying 172 differentially expressed proteins upon clpX interruption. The results provide a comprehensive ClpX-regulated protein network and new candidates for future functional studies on ClpX in cyanobacteria.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Pia H. Moisander, Meaghan C. Daley, Katyanne M. Shoemaker, Vaishnavi Kolte, Gaurav Sharma, Kelsey Garlick
Summary: This study unexpectedly discovered mass accumulations of Hydrocoleum sp. outside the known habitat range in a coastal lagoon in the Western temperate North Atlantic Ocean, raising intriguing questions about diazotrophic cyanobacterial adaptations and transitions on the benthic-pelagic continuum.
JOURNAL OF PHYCOLOGY
(2022)
Article
Evolutionary Biology
Theophile Grebert, Laurence Garczarek, Vincent Daubin, Florian Humily, Dominique Marie, Morgane Ratin, Alban Devailly, Gregory K. Farrant, Isabelle Mary, Daniella Mella-Flores, Gwenn Tanguy, Karine Labadie, Patrick Wincker, David M. Kehoe, Frederic Partensky
Summary: Synechococcus cyanobacteria play a significant role in marine primary productivity, and their light-harvesting complexes (PBS) exhibit genomic variability that contributes to the wide diversity of pigment types. The study reveals the evolutionary history and allelic exchange of PBS rod genes, and highlights the importance of incomplete lineage sorting in maintaining pigment diversity in different Synechococcus lineages.
GENOME BIOLOGY AND EVOLUTION
(2022)
Article
Microbiology
Jan Karlsen, Johannes Asplund-Samuelsson, Michael Jahn, Dora Vitay, Elton P. Hudson
Summary: Metabolic engineered cyanobacteria have the potential to convert CO2 into renewable fuels and chemicals to reduce anthropogenic CO2 emissions. This study aims to understand the metabolic regulation in cyanobacteria, and experimental analysis reveals that translational regulation does not dampen protein oscillations, which can be attributed to slow protein turnover.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Rosemary E. Maher, Merita Maatta, Robert J. Beynon, Henna P. Laurila, Paul S. McNamara, Minna M. Rajamaki
Summary: Label-free proteomics allows discrimination between CIPF and healthy WHWTs, but does not provide clear evidence for gastrointestinal aspiration.
BMC VETERINARY RESEARCH
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Caroline A. Staunton, Euan D. Owen, Kay Hemmings, Malcolm J. Jackson, Richard Barrett-Jolley, Dean Hammond, Robert J. Beynon, Anne McArdle
Article
Biochemical Research Methods
Dean E. Hammond, Deborah M. Simpson, Catarina Franco, Marina Wright Muelas, John Waters, R. W. Ludwig, Mark C. Prescott, Jane L. Hurst, Robert J. Beynon, Edward Lau
Summary: This study compares the turnover rates of proteins in four mouse tissues using different labeling precursors, and finds that both methods yield similar results for long-lived proteins but require compensation for short-lived proteins. The determination of precursor enrichment kinetics greatly influences the numerical values of the derived protein turnover rates.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Microbiology
Maria Agustina Dominguez-Martin, Antonio Lopez-Lozano, Yesica Melero-Rubio, Guadalupe Gomez-Baena, Juan Andres Jimenez-Estrada, Kateryna Kukil, Jesus Diez, Jose Manuel Garcia-Fernandeza
Summary: Marine Synechococcus and Prochlorococcus are important contributors to primary production on Earth, and their distribution is influenced by various factors. This study reveals a specific response of Synechococcus to nanomolar concentrations of nitrate, which may provide an evolutionary advantage for this organism over Prochlorococcus in the natural environment.
MICROBIOLOGY SPECTRUM
(2022)
Review
Microbiology
J. Diez, A. Lopez-Lozano, M. A. Dominguez-Martin, G. Gomez-Baena, M. C. Munoz-Marin, Y. Melero-Rubio, J. M. Garcia-Fernandez
Summary: Marine picocyanobacteria, such as Prochlorococcus and Synechococcus, have developed unique adaptive mechanisms in nitrogen metabolism to thrive in oligotrophic oceans, including strategies such as reducing GC and protein contents, utilizing truncated proteins, and perceiving nanomolar nitrate concentrations. These adaptations contrast with the knowledge obtained in freshwater cyanobacteria and are essential for the ecological success of these microorganisms.
FEMS MICROBIOLOGY REVIEWS
(2023)
Article
Biology
Iris Wagner, Linda Grigoraki, Peter Enevoldson, Michael Clarkson, Sam Jones, Jane L. L. Hurst, Robert J. J. Beynon, Hilary Ranson
Summary: The rapid evaporative ionisation mass spectrometry (REIMS) method can accurately identify the species and age of mosquitoes, with high accuracy in both laboratory-reared and wild populations.
Article
Biochemical Research Methods
Martin Rusilowicz, David W. Newman, Declan R. Creamer, James Johnson, Kareena Adair, Victoria M. Harman, Chris M. Grant, Robert J. Beynon, Simon J. Hubbard
Summary: Protein quantitation via mass spectrometry requires the use of peptide proxies, and accurate quantitation often relies on the addition of an external standard. AlacatDesigner is a tool that assists in selecting suitable peptide candidates for recombinant protein standards. It considers factors such as target protein, existing databases, literature occurrence, potential post-translational modifications, and ionization potential within the mass spectrometer.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Microbiology
Jose Angel Moreno-Cabezuelo, Guadalupe Gomez-Baena, Jesus Diez, Jose Manuel Garcia-Fernandez
Summary: This study compared the effects of different concentrations of glucose on the proteome and metabolome of Synechococcus sp. and Prochlorococcus sp. strains. The results showed that these cyanobacteria metabolize glucose primarily through oxidative pentoses and Calvin pathways, and no evidence was found for the involvement of the Entner-Doudoroff pathway. Differences in glucose effects were observed between genera and between Prochlorococcus MED4 and SS120 strains, possibly due to their specific adaptations to the environment. Additionally, fermentation was observed in Prochlorococcus sp. strain SS120 and Synechococcus sp. strain WH8102 after 5 mM glucose addition.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Jose Angel Moreno-Cabezuelo, Maria del Carmen Munoz-Marin, Antonio Lopez-Lozano, Diogo Athayde, Ana Simon-Garcia, Jesus Diez, Margarida Archer, Federico M. Issoglio, Jose Manuel Garcia-Fernandez
Summary: The expression and mutation of Prochlorococcus marinus glcH gene in Escherichia coli were studied. Structural modeling and mutagenesis experiments showed that amino acid residues D8, N117, R141, and W348 play important roles in glucose transport. These studies provide insights into the high affinity and multiphasic kinetics of the glucose transporter, paving the way for further analysis.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Article
Chemistry, Medicinal
Ines Ventura, Victoria Harman, Robert J. Beynon, Romana Santos
Summary: Biomedical adhesives still struggle with strong adhesion in wet environments. Marine invertebrates' biological adhesives show promising characteristics for underwater biomimetic adhesives. Through analysis of sea urchin Paracentrotus lividus adhesive proteins, researchers identified glycosylated protein candidates. The deeper characterization of these adhesive glycoproteins advances the development of sea urchin-inspired bioadhesives.
Article
Multidisciplinary Sciences
Guadalupe Gomez-Baena, Kieran C. Pounder, Josiah O. Halstead, Sarah A. Roberts, Amanda J. Davidson, Mark Prescott, Robert J. Beynon, Jane L. Hurst
Summary: Chemical communication by females in social species, such as laboratory rats, is important for competition and cooperation between females in determining reproductive success. Female rats target their deployment of scent information differently based on their sexual receptivity and the genetic identity of the individuals in the environment. The scent marks of female rats contain a complex mix of proteins, with clitoral gland secretion being dominant, and play a key role in female communication.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemical Research Methods
Leandro Xavier Neves, R. Alan Wilson, Philip Brownridge, Stephen W. W. Holman, Victoria M. M. Harman, Claire E. E. Eyers, Robert J. J. Beynon, William Castro-Borges
Summary: In this study, a detailed method for preserving the proteome of adult Schistosoma mansoni worms was described in order to enrich the tissues associated with the parasite's alimentary tract. The methodology employed label-free and QconCAT-based absolute quantification to detect S. mansoni oesophageal gland products. By stabilizing the proteome and minimizing sample degradation during dissection, the hidden proteome of target tissues, which are not readily available from total lysates due to their small volumes, could be accessed. This protocol can be used for the discovery of proteins with potential diagnostic and therapeutic utility in other Schistosoma species lacking quantitative proteomics characterization of specialized tissues.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2023)
Article
Biology
Jonathan P. Green, Catarina Franco, Amanda J. Davidson, Vicki Lee, Paula Stockley, Robert J. Beynon, Jane L. Hurst
Summary: Breeding females can cooperatively rear their offspring, but this may lead to risks of exploitation. In lactating mammals, communal rearing is more common among close relatives. This study used a dual isotopic tracer approach to track milk allocation in familiar pairs of sisters and unrelated house mice. The results showed that closely related pairs had lower energy demand and the pups had better access to non-maternal milk. However, there was a greater energetic cost for females when rearing offspring with an unrelated partner.
COMMUNICATIONS BIOLOGY
(2023)