期刊
FRONTIERS IN MICROBIOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2017.01573
关键词
oil field; seawater; sulfate reduction; nitrate reduction; microbial community
类别
资金
- NSERC
- BP America Production Co.
- Baker Hughes Canada
- Computer Modeling Group Limited
- ConocoPhillips Company
- Dow Microbial Control
- Enbridge Inc.
- Enerplus Corporation
- Intertek Commercial Microbiology
- Oil Search (PNG) Limited
- Shell Global Solutions International
- Suncor Energy Inc.
- Yara Norge AS
- Alberta Innovates
Oil fields can experience souring, the reduction of sulfate to sulfide by sulfate-reducing microorganisms. At the Terra Nova oil field near Canada's east coast, with a reservoir temperature of 95 degrees C, souring was indicated by increased hydrogen sulfide in produced waters (PW). Microbial community analysis by 16S rRNA gene sequencing showed the hyperthermophilic sulfate-reducing archaeon Archaeoglobus in Terra Nova PWs. Growth enrichments in sulfate-containing media at 55-70 degrees C with lactate or volatile fatty acids yielded the thermophilic sulfate-reducing bacterium (SRB) Desulfotomaculum. Enrichments at 30-45 degrees C in nitrate-containing media indicated the presence of mesophilic nitrate-reducing bacteria (NRB), which reduce nitrate without accumulation of nitrite, likely to N-2. Thermophilic NRB (tNRB) of the genera Marinobacter and Geobacillus were detected and isolated at 30-50 degrees C and 40-65 degrees C, respectively, and only reduced nitrate to nitrite. Added nitrite strongly inhibited the isolated thermophilic SRB (tSRB) and tNRB and SRB could not be maintained in co-culture. Inhibition of tSRB by nitrate in batch and continuous cultures required inoculation with tNRB. The results suggest that nitrate injected into Terra Nova is reduced to N-2 at temperatures up to 45 degrees C but to nitrite only in zones from 45 to 65 degrees C. Since the hotter zones of the reservoir (65-80 degrees C) are inhabited by thermophilic and hyperthermophilic sulfate reducers, souring at these temperatures might be prevented by nitrite production if nitrate-reducing zones of the system could be maintained at 45-65 degrees C.
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