Article
Microbiology
Yingying Kong, Chao Li, Hangfei Chen, Wei Zheng, Qingyang Sun, Xinyou Xie, Jun Zhang, Zhi Ruan
Summary: This study isolated a pair of carbapenem-resistant Klebsiella pneumoniae isolates from the same patient before and after colistin treatment, named KP1-1 and KP1-2. The Gln30* alteration in KP1-2 was found to be responsible for colistin resistance, and complementation with wild-type mgrB gene restored colistin susceptibility. Overexpression of phoP, phoQ, and pmrD genes in KP1-2 compared to KP1-1 confirmed the role of MgrB alterations in increased expression levels of these genes, providing direct in vivo evidence for colistin resistance in K. pneumoniae clinical strains.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Xi Jin, Qiong Chen, Fang Shen, Yan Jiang, Xueqing Wu, Xiaoting Hua, Ying Fu, Yunsong Yu
Summary: This study reported a case of hv-CRKP-associated infection and revealed the in-host evolution of resistance to tigecycline and polymyxin during clinical therapy. The results suggest that the hypervirulent ST11-KL64 clone poses a potential threat to anti-infection treatment with rapid and diverse evolution of resistance.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Microbiology
Jie Sheng, Rory Cave, Mary M. Ter-Stepanyan, Nune Kotsinyan, Jiazhen Chen, Li Zhang, Taijiao Jiang, Hermine V. Mkrtchyan
Summary: This study reports the first whole-genome sequencing of a multidrug-resistant Klebsiella pneumoniae isolate from Armenia. The isolate exhibited resistance to multiple antibiotics and carried several antibiotic resistance genes. The study emphasizes the importance of genomic surveillance and implementation of infection prevention and control measures for emerging pathogens.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Angela W. Muraya, Cecilia Kyany'a, Shahiid Kiyaga, Hunter J. Smith, Caleb Kibet, Melissa J. Martin, Josephine Kimani, Lillian Musila
Summary: This study analyzed the epidemiology, resistance, and virulence genes of Klebsiella pneumoniae strains in Kenya. The results revealed diverse resistance and virulence genes in both clinical and hospital environmental isolates, highlighting the potential for unmanageable infection outbreaks.
Article
Microbiology
Mengli Tang, Jun Li, Zhaojun Liu, Fengjun Xia, Changhang Min, Yongmei Hu, Haichen Wang, Mingxiang Zou
Summary: This study collected eight polymyxin B-resistant K. pneumoniae isolates from a Chinese teaching hospital and analyzed their resistance and virulence characteristics. It was found that these bacteria were highly virulent and transmissible, posing a serious challenge to public health in China.
Article
Virology
Zhen Liang, Yun-Long Shi, Yuan Peng, Chen Xu, Cheng Zhang, Yu Chen, Xiao-Qiang Luo, Qi-Meng Li, Cheng-Lei Zhao, Jie Lei, Zhi-Qiang Yuan, Yi-Zhi Peng, Bao-Qiang Song, Ya-Li Gong
Summary: This study isolated and evaluated a phage BL02 that is resistant to both carbapenem and polymyxin-B in CRKP, which poses a threat to public health in Southwestern China. BL02 showed high lytic activity against CRKP isolates and has potential applications in treating polymyxin-B resistant K. pneumoniae.
Article
Pharmacology & Pharmacy
Haibin Li, Lang Sun, Han Qiao, Zongti Sun, Penghe Wang, Chunyang Xie, Xinxin Hu, Tongying Nie, Xinyi Yang, Guoqing Li, Youwen Zhang, Xiukun Wang, Zhuorong Li, Jiandong Jiang, Congran Li, Xuefu You
Summary: Polymyxin B and polymyxin E are last-line antibiotics for multidrug-resistant Gram-negative infections, but resistance is increasing. Polymyxin S2, a new analogue, shows high antibacterial activity and lower toxicity. Resistance mechanism of S2 is similar to polymyxin B and polymyxin E.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Infectious Diseases
Yunjia Song, Jinge Yang, Yingjiao Ju, Dejun Liu, Yang Wang, Xutao Sun, Yuan Lv
Summary: This study generated a draft genome sequence for a CoRKp isolate, revealing that the colistin resistance was mainly due to the total deletion of the mgrB gene. This is the first report of the whole genome sequence of such an unusual colistin-resistant strain.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Microbiology
Xinyi Chen, Jingjing Tian, Can Luo, Xiaofan Wang, Xianping Li, Min Wang
Summary: This study investigates the key biological processes associated with polymyxin B (PB) resistance in carbapenem-resistant Klebsiella pneumoniae (CRKP) using quantitative proteomics and metabolomics analysis. The study finds that membrane remodeling and inhibition of central carbon metabolism contribute to the development of PB resistance in K. pneumoniae. Additionally, PB treatment leads to increased reactive oxygen species (ROS) levels and abnormal lipid metabolism in K. pneumoniae. These findings enhance our understanding of the mechanisms of PB resistance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Manuela Tietgen, Lisa Sedlaczek, Paul G. G. Higgins, Heike Kaspar, Christa Ewers, Stephan Goettig
Summary: This study explores common colistin resistance mechanisms in Klebsiella pneumoniae isolates from animals, finding that the resistance mechanisms were diverse alterations of MgrB in both human and animal isolates. Phylogenetic analysis indicated that colistin resistance mechanisms developed independently in human and animal isolates. Further analysis of resistance mechanisms is crucial due to the limited antibiotics available to treat infections with multidrug-resistant bacteria.
Article
Microbiology
Shuying Chen, Peiyao Zhou, Chunyang Wu, Jie Wang, Ying Zhou, Jiao Zhang, Bingjie Wang, Huilin Zhao, Lulin Rao, Meilan Li, Fangyou Yu, Chunchan Lin
Summary: The increasing prevalence of multidrug-resistant Gram-negative bacteria poses a major threat to public health. This study investigated the synergistic effect of polymyxin B and fusidic acid in killing bacteria. The results showed that the combination therapy was more effective than monotherapy and increased the sensitivity of multidrug-resistant strains to polymyxin B.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Alexandra Chiaverini, Alessandra Cornacchia, Gabriella Centorotola, Elga Ersilia Tieri, Nadia Sulli, Ilaria Del Matto, Giorgio Iannitto, Domenico Petrone, Antonio Petrini, Francesco Pomilio
Summary: This study investigates the occurrence of Klebsiella pneumoniae in wildlife and its antimicrobial resistance. The results show the presence of resistant strains in wild animals, indicating a link between humans, domestic animals, and wildlife. This highlights the lack of knowledge about this ecological context and underestimation of potential health risks.
Article
Infectious Diseases
Elsa de la Cadena, Maria Fernanda Mojica, Juan Carlos Garcia-Betancur, Tobias Manuel Appel, Jessica Porras, Christian Jose Pallares, Juan Sebastian Solano-Gutierrez, Laura J. Rojas, Maria Virginia Villegas
Summary: A study in Colombia identified clonal dissemination of polymyxin and carbapenem-resistant K. pneumoniae isolates, mainly associated with CG258 and bla(KPC-3), highlighting limited therapeutic options for carbapenem-resistant strains.
Article
Infectious Diseases
Qi Zhang, Wenjuan Yan, Yingjie Zhu, Nan Jing, Shanmei Wang, Youhua Yuan, Bing Ma, Junhong Xu, Yafei Chu, Jiangfeng Zhang, Qiong Ma, Baoya Wang, Wenbo Xu, Liqiang Zhu, Ying Sun, Caiqin Shi, Juan Fang, Yi Li, Shengqun Liu
Summary: This study evaluated the performance of five widespread commercial products for colistin and polymyxin B susceptibility testing in China. The results showed that Vitek 2 and Phoenix M50 performed well for mcr-1-positive Escherichia coli, while DL-96II, MA120, and the POL E-strip performed poorly. All systems showed unsatisfactory performance for mcr-8-positive Klebsiella pneumoniae isolates.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Microbiology
Radwa Abdelwahab, Munirah M. Alhammadi, Ehsan A. Hassan, Entsar H. Ahmed, Nagla H. Abu-Faddan, Enas A. Daef, Stephen J. W. Busby, Douglas F. Browning
Summary: Klebsiella pneumoniae is an important human pathogen in Egypt with high levels of antibiotic resistance, and strain KPE16 isolated from a child in Assiut carries multiple antimicrobial resistance genes, including a bla(NDM-1) carbapenemase and extended spectrum beta-lactamase genes. Comparison with other Egyptian isolates reveals common plasmids and resistance genes, suggesting a potential source of antibiotic resistance worldwide.
Article
Infectious Diseases
Chaitra Shankar, Susmitha Karunasree, Abi Manesh, Balaji Veeraraghavan
MICROBIAL DRUG RESISTANCE
(2019)
Article
Infectious Diseases
Chaitra Shankar, Agila Kumari Pragasam, Shalini Anandan, Balaji Veeraraghavan
MICROBIAL DRUG RESISTANCE
(2019)
Article
Public, Environmental & Occupational Health
Chaitra Shankar, Sathish Kumar, Manigandan Venkatesan, Balaji Veeraraghavan
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2019)
Article
Microbiology
Chaitra Shankar, Purva Mathur, Manigandan Venkatesan, Agila Kumari Pragasam, Shalini Anandan, Surbhi Khurana, Balaji Veeraraghavan
Article
Immunology
Chaitra Shankar, Dhiviya Prabaa Muthuirulandi Sethuvel, Ayyan Raj Neeravi, Manigandan Venkatesan, Naveen Kumar Devanga Ragupathi, Shalini Anandan, Balaji Veeraraghavan
MICROBIAL PATHOGENESIS
(2020)
Meeting Abstract
Infectious Diseases
C. Shankar, P. Mathur, J. J. Jacob, C. Rodrigues, K. Walia, D. S. Chitnis, B. Veeraraghavan
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Immunology
Chaitra Shankar, Jobin John Jacob, Karthick Vasudevan, Rohit Biswas, Abi Manesh, Dhiviya Prabaa Muthuirulandi Sethuvel, Santosh Varughese, Indranil Biswas, Balaji Veeraraghavan
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2020)
Article
Infectious Diseases
Chaitra Shankar, Jobin John Jacob, Suganya Gopal Sugumar, Lavanya Natarajan, Camilla Rodrigues, Purva Mathur, Dip Narayan Mukherjee, Anita Sharma, D. S. Chitnis, Anudita Bharagava, Abi Manesh, Karthik Gunasekaran, Balaji Veeraraghavan
Summary: This study identified a high prevalence of multidrug-resistant K. pneumoniae in India, with OXA48-like carbapenemases being the main drivers of resistance. The predominant sequence type among OXA48-like producers was ST231, while NDM producers and NDM+OXA48-like producers were primarily associated with ST14. Additionally, a significant portion of CR K. pneumoniae exhibited colistin resistance, with plasmid analysis revealing diverse associations between carbapenemase-carrying genes and plasmid types.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Immunology
Chaitra Shankar, Soumya Basu, Binesh Lal, Sathiya Shanmugam, Karthick Vasudevan, Purva Mathur, Sudha Ramaiah, Anand Anbarasu, Balaji Veeraraghavan
Summary: The incidence of hypervirulent carbapenem-resistant Klebsiella pneumoniae (hvCRKp) is increasing globally, with high-risk clones acquiring a virulence plasmid and showing phenotypic changes. Resistance to carbapenems is mainly due to OXA-232 and NDM carbapenemases. The presence of non-functional RmpA2 challenges phenotypic screening, highlighting the need for reliable markers for identifying hvCRKp.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Chaitra Shankar, Karthick Vasudevan, Jobin John Jacob, Stephen Baker, Barney J. Isaac, Ayyan Raj Neeravi, Dhiviya Prabaa Muthuirulandi Sethuvel, Biju George, Balaji Veeraraghavan
Summary: This study analyzed a collection of MDR-HvKp (ST2096) strains carrying antimicrobial resistance and virulence genes, and found that they carried a large hybrid plasmid. The results suggest that MDR-HvKp is no longer limited to specific clones and continues to emerge globally, posing a major public health threat.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Abdoul Hamide, Rima Mahapatra, Adrian Keith Noronha, Balamurugesan Kandan, Chaitra Shankar, Balaji Veeraraghavan
Summary: Hypervirulent Klebsiella pneumoniae infection, commonly found in young healthy adults in Southeast Asia, is a rare but serious disease. This report describes a patient with uncontrolled diabetes mellitus who presented with symptoms of liver abscess and infective endocarditis. The patient ultimately died from the infection.
Meeting Abstract
Infectious Diseases
Balaji Veeraraghavan, Chaitra Shankar, Saranya Vijayakumar, Harthi Ragothaman, Abirami Shankar, Binesh Lal, Abi Manesh
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Review
Immunology
Balaji Veeraraghavan, Agila Kumari Pragasam, Yamuna Devi Bakthavatchalam, Shalini Anandan, Subramanian Swaminathan, Balasubramanian Sundaram
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2019)
Article
Immunology
Chaitra Shankar, Agile Kumari Pragasam, Balaji Veeraraghavan, Anushree Amladi
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2019)
Article
Immunology
Agila Kumari Pragasam, Balaji Veeraraghavan, Baby Abirami Shankar, Yamuna Devi Bakthavatchalam, Alice Mathuram, Biju George, Binila Chacko, Pritish Korula, Shalini Anandan
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2019)