期刊
FRONTIERS IN MICROBIOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2017.02418
关键词
LA-MRSA CC398; biocide; prophage; Phi Sa3; phage transfer
类别
资金
- Danish Research Council Sapere Aude grant
- Deutsche Forschungsgemeinschaft [GRK1708]
- Faculty of Health and Medical Sciences, University of Copenhagen
- Lundbeck Foundation [R140-2013-13496, R77-2010-6772] Funding Source: researchfish
- NNF Center for Biosustainability [Bacterial Synthetic Biology] Funding Source: researchfish
- Novo Nordisk Fonden [NNF10CC1016517, NNF13SA0009311, NNF13SA0006019, NNF14OC0011335] Funding Source: researchfish
Human strains of Staphylococcus aureus commonly carry the bacteriophage Phi Sa3 that encodes immune evasion factors. Recently, this prophage has been found in livestock-associated, methicillin resistant S. aureus (MRSA) CC398 strains where it may promote human colonization. Here, we have addressed if exposure to biocidal products induces phage transfer, and find that during co-culture, Phi 13 from strain 8325, belonging to Phi Sa3 group, is induced and transferred from a human strain to LA-MRSA CC398 when exposed to sub-lethal concentrations of commercial biocides containing hydrogen peroxide. Integration of Phi Sa3 in LA-MRSA CC398 occurs at multiple positions and the integration site influences the stability of the prophage. We did not observe integration in hlb encoding b -hemolysin that contains the preferred Phi Sa3 attachment site in human strains, and we demonstrate that this is due to allelic variation in CC398 strains that disrupts the phage attachment site, but not the expression of b-hemolysin. Our results show that hydrogen peroxide present in biocidal products stimulate transfer of Phi Sa3 from human to LA-MRSA CC398 strains and that in these strains prophage stability depends on the integration site. Knowledge of Phi Sa3 transfer and stability between human and livestock strains may lead to new intervention measures directed at reducing human infection by LA-MRSA strains.
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