期刊
FRONTIERS IN MICROBIOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2017.01888
关键词
metagenome; virome; outbreak; RNAlater; Paramyxovirus
类别
Outbreaks of infectious diseases may occur in animal and human populations; this underlines the need for suitable One Health approaches. During outbreak situations, straightforward identification of etiological agents is indispensable for taking countermeasures. A recently published protocol for metagenomic virus detection in clinical specimens (TUViD-VM) was developed for snap-frozen tissues which can be challenging to obtain. Here, we describe the use of RNALater (R)-treated tissue at ambient temperatures for virome sequencing. This study demonstrates that samples stored in RNALater (R) buffer yield similar results to those stored snap-frozen.
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