Article
Environmental Sciences
Mingwei Shi, Xiaotong Xiao, Haili Ma, Li Li, Yujue Wang, Caixia Dong, Yang Ding, Meixun Zhao
Summary: This study investigated the sources and distribution patterns of ladderanes in suspended particulates from estuaries and the inner area of Laizhou Bay in North China. It proposed a new index, R-lad, to evaluate the sources of ladderanes. The results showed that ladderanes mainly originated from terrestrial input and in situ production in oxygen-depleted estuaries, rather than the marine environment. Additionally, the study revealed that high concentrations of ladderanes synthesized by terrestrial anammox bacteria could significantly contribute to coastal seas, suggesting an underestimation of the anammox process in river-estuary-bay systems under anthropogenic influences.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Tesshin Uegaki, Taisei Takei, Shuhei Yamaguchi, Keisuke Fujiyama, Yusuke Sato, Tomoya Hino, Shingo Nagano
Summary: This study reveals the crystal structure of a SAM-dependent methyltransferase (AmxMT1) from anammox bacteria, providing evidence that the enzyme transfers methyl group from SAM to the substrate connected to AmxACP, resulting in the production of ladderane lipids. Although the enzymes responsible for constructing the ladder-like structure are still unknown, this study supports the involvement of SAM-dependent enzymes in processing biosynthetic intermediates connected to AmxACP for ladderane lipid synthesis in anammox bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agricultural Engineering
Yunlong Su, Rui Du, Jiao Wang, Xiyao Li, Qiong Zhang, Xiaofei Xue, Yongzhen Peng
Summary: In this study, anammox bacteria were efficiently enriched and retained in a suspended sludge system for mainstream wastewater treatment. The system provided favorable conditions for the growth of anammox bacteria, including low nitrogen loading rate, low ammonia and nitrate concentrations, and anoxic environment. This research demonstrated the feasibility and potential of applying anammox technology without specific sludge seeding in mainstream wastewater treatment.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Zijian Zhu, Jiafu Qin, Zhenguo Chen, Yongxing Chen, Haochuan Chen, Xiaojun Wang
Summary: In this study, the thiosulfate-driven denitrification and Anammox process (TDDA) was successfully started and operated continuously for 146 days, achieving good nitrogen removal efficiency. The study also revealed the positive role of the Sulfammox process in nitrogen and sulfur removal, and identified the bacteria associated with this process through high-throughput analysis.
ENVIRONMENTAL RESEARCH
(2022)
Review
Environmental Sciences
Shenbin Cao, Rui Du, Yan Zhou
Summary: This study reviews the strategies to establish a competition balance between anammox and denitrification processes by analyzing the kinetics of anammox and denitrifying microorganisms, discussing the favorable conditions in establishing the coupled process, and evaluating the system recovery approaches. Additionally, the dynamics of the microbial communities in response to the organic matters are also presented.
CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2021)
Article
Engineering, Environmental
Ehui Tan, Ting-Chang Hsu, Wenbin Zou, Xiuli Yan, Zhixiong Huang, Bin Chen, Yongkai Chang, Zhenzhen Zheng, Liwei Zheng, Min Xu, Li Tian, Shuh-Ji Kao
Summary: The study reveals that sedimentary denitrification is the main nitrogen removal pathway in estuarine environments, with a minor contribution from anammox. Estuarine sediments play a relatively weak role in eliminating nitrogen input, while the excess nitrogen from rivers will eventually be removed through various biological processes.
Article
Agricultural Engineering
Yueru Zhao, Qiong Zhang, Yi Peng, Yongzhen Peng, Xiyao Li, Hao Jiang
Summary: This study achieved efficient operation of Anammox treating low ammonia sewage through the PND-SAD process, with key measures including control of dissolved oxygen concentration and monitoring of nitrate concentration. In the system, the nitrite accumulation rate reached 97.6% and nitrogen removal efficiency was as high as 97.3%.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Environmental
Vojtech Kouba, Kamila Hurkova, Klara Navratilova, Dana Kok, Andrea Benakova, Michele Laureni, Patricie Vodickova, Tomas Podzimek, Petra Lipovova, Laura van Niftrik, Jana Hajslova, Mark C. M. van Loosdrecht, David Gregory Weissbrodt, Jan Bartacek
Summary: In the study of the performance of anammox reactions under low-temperature conditions, membrane composition and cultivation temperature were found to be closely related to the activity of anammox biomasses. The adaptation of mesophilic cultures to low-temperature conditions may take several months or even up to 5 years. An interesting finding in these studies is that biomass enriched in the marine genus Candidatus Scalindua showed outstanding potential for nitrogen removal from cold streams.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Green & Sustainable Science & Technology
Parin Izadi, Pooja Sinha, Mehran Andalib, Caroline Samberger, Geno Lehman, Katerina Messologitis, Joseph Jacangelo
Summary: In recent years, wastewater treatment plants have been challenged to adopt innovative processes due to nutrient management and stringent regulatory limits. The Partial Denitrification Anammox (PDNA) process has successfully transitioned from pilot scale to full-scale within a short period of time, offering a sustainable solution with significant savings in carbon, energy, and footprint. This review examines the recent advances in the PD process and its coupling to the anammox process, analyzing the fundamental mechanisms and operational strategies responsible for stable PDNA process. It also highlights the importance of controlling nitrate to nitrite conversion and the neglected significance of operational factors such as bacterial ecology shift, C/N ratio, SRT decoupling, and feast-famine conditions in achieving stable PDNA process.
JOURNAL OF CLEANER PRODUCTION
(2023)
Review
Engineering, Environmental
Yang-Fan Deng, Fei-xiang Zan, Hao Huang, Di Wu, Wen-tao Tang, Guang-Hao Chen
Summary: This review systematically summarizes and discusses the sulfur-based denitrification with anammox (SDA) process, including its biochemistry, biokinetic reactions, reactor performance, and application. The dominant functional bacteria and microbial interactions in the SDA process are also discussed. The paper provides an in-depth insight into the coupling mechanism of SDA and its potential application in biological nitrogen removal.
Article
Engineering, Environmental
Depeng Wang, Qi Luo, Kailong Huang, Xu-Xiang Zhang
Summary: This study investigated the assembly processes and interactions of functional bacteria in anammox and denitrification coupling systems using lab-scale up-flow bioreactors. The results showed that the community composition of functional bacteria differed between the systems, and the community assembly was mainly governed by stochastic processes and homogeneous selection. Moreover, the network structures of anammox and denitrifying bacteria varied between the systems.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Hannah Waterhouse, Bhavna Arora, Nicolas F. Spycher, Peter S. Nico, Craig Ulrich, Helen E. Dahlke, William R. Horwath
Summary: The study used a reactive transport model to analyze the impact of Agricultural Managed Aquifer Recharge (AgMAR) on nitrate (NO3-) leaching to groundwater under different management strategies. Simulation results indicated that finer textured sediments are crucial for providing conditions needed for denitrification, while applying water all-at-once may exacerbate groundwater quality issues but also increase denitrification within the vadose zone. The research concludes that AgMAR can be designed to enhance denitrification in the subsurface and reduce NO3- leaching to groundwater.
WATER RESOURCES RESEARCH
(2021)
Review
Agricultural Engineering
Yujie Chen, Guangze Guo, Yu -You Li
Summary: This review introduces the application of anaerobic ammonia oxidation (anammox) process in nitrogen removal and summarizes methods to improve nitrogen removal rate. Recently developed integrated systems aim to enhance nitrogen removal rate and efficiency, providing new insights for achieving efficient nitrogen removal.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Environmental
Hafiz Adeel Ahmad, Shakeel Ahmad, Linjie Gao, Sherif Ismail, Zhibin Wang, Amro El-Baz, Shou-Qing Ni
Summary: This study establishes a stable connection between PD and anammox, achieving continuous nitrite generation and stable denitrification process under the influence of a low-strength magnetic field. The nitrogen removal efficiency is improved, indicating the potential of this technique for energy-efficient and lower carbon nitrogen removal from wastewater.
Article
Engineering, Environmental
Lijie Zhou, Bikai Zhao, Wei-Qin Zhuang
Summary: The double-edged sword effects of Dissimilatory nitrate reduction to ammonium (DNRA) bacteria on anammox bacteria were investigated. It was found that the overgrowth of DNRA bacteria had a negative impact on the nitrogen removal efficiency of anammox bacteria.
Article
Chemistry, Physical
Thomas Ritschel, Katharina Lehmann, Michaela Brunzel, Juergen Vitz, Ivo Nischang, Ulrich S. Schubert, Kai U. Totsche
Summary: The study demonstrates that tailor-made synthetic polymers can accurately predict the transport behavior of PEG in various porous media, indicating the potential of functionalized PEGs as customizable and non-toxic tracers.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Geosciences, Multidisciplinary
Nico Ueberschaar, Katharina Lehmann, Stefanie Meyer, Christian Zerfass, Beate Michalzik, Kai Uwe Totsche, Georg Pohnert
Summary: The passage of surface-sourced organic compounds in infiltrating waters and percolates undergoes transformations through biotic and abiotic processes, leading to changing chemical environments in subsurface compartments. Different types of lysimeters are available for collecting soil solution samples, and solid phase extraction followed by GC-MS analysis can effectively reduce background signals. Multivariate statistics can be used for monitoring chemical differences in various land use sites, allowing for the detection of organic compounds such as sugars, fatty acids, and phenolic acids.
FRONTIERS IN EARTH SCIENCE
(2021)
Article
Biochemical Research Methods
Annika Blohm, Swatantar Kumar, Andreas Knebl, Martina Herrmann, Kirsten Kuesel, Jurgen Popp, Torsten Frosch
Summary: This study aimed to investigate substrate preferences for two mixotrophic denitrifying bacterial strains, Acidovorax delafieldii and Hydrogenophaga taeniospiralis, under heterotrophic, autotrophic, or mixotrophic conditions. The results demonstrated that Acidovorax delafieldii preferentially performed heterotrophic denitrification in the mixotrophic sub-experiments, while Hydrogenophaga taeniospiralis preferred autotrophic denitrification in the mixotrophic incubation. Advanced Raman multi-gas analysis proved useful in studying substrate use and electron donor preferences in denitrification based on comprehensive quantification of complex microbial gas exchange processes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Tom Guhra, Thomas Ritschel, Kai Uwe Totsche
Summary: A comprehensive modeling study on the evolution of an aggregated three-dimensional structure due to diffusion, surface interactions, and gravity revealed three mechanisms of aggregation and showed how the balance of different interactions determines the rate of aggregation. The 3D visualization tool allowed tracking of subtle interplay of mechanisms and dynamic hierarchical self-assembly of aggregates.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Engineering, Environmental
Lijuan Yan, Syrie M. Hermans, Kai Uwe Totsche, Robert Lehmann, Martina Herrmann, Kirsten Kuesel
Summary: Time series analyses are crucial in understanding the temporal dynamics of bacterial communities in groundwater. The study revealed that bacterial communities in shallow fractured rock groundwater are not stable over time, with the dissimilarity increasing with successive recharge events. The impact of recharge events on the groundwater microbiome is linked to the strength of recharge and local environmental selection, highlighting the unique driving factors for microbial patterns in groundwater.
Article
Soil Science
Simon Zech, Thomas Ritschel, Nadja Ray, Kai Uwe Totsche, Alexander Prechtel
Summary: Microaggregates, as hot spots of microbial activity, pose challenges in direct observation due to their small scale. Mathematical models combining organic matter transport mechanisms and turnover processes can help understand soil microbial dynamics at these scales. This study showed that the heterogeneous distribution of substrate and bacteria in microaggregates impacts overall biodegradation kinetics and CO2 output, with smaller microaggregate scales having a more significant impact.
Article
Environmental Sciences
Marie Mollenkopf, Andreas Fritzsche, Daniela Montalvo, Maria Diez-Ortiz, Veronica Gonzalez-Andres, Erik Smolders, Rainer Meckenstock, Kai Uwe Totsche
Summary: The research shows that the adsorption affinity of HA-Goe towards arsenic, copper, zinc, nickel, and cobalt decreases, and the immobilization effect on Daphnia magna weakens as the exposure time in groundwater increases.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Soil Science
Tom Guhra, Katharina Stolze, Kai Uwe Totsche
Summary: Soil organisms play important roles as ecosystem engineers in promoting soil aggregation, enhancing soil quality, and nutrient cycling. The complex composition of biogenically excreted organic matter can either promote or inhibit aggregation, affecting aggregate formation, stability, and transport through various functional roles.
SOIL BIOLOGY & BIOCHEMISTRY
(2022)
Article
Soil Science
Katharina Stolze, Andrew D. Barnes, Nico Eisenhauer, Kai U. Totsche
Summary: This study investigates the linkage between earthworms, roots, and soil properties with the number of biopores. It reveals complex relationships among roots, earthworms, biopores, and soil properties, with land-use intensity playing a significant role. The occurrence, frequency, and persistence of biopores are found to be influenced by these interdependencies.
Article
Engineering, Environmental
Christian Zerfass, Robert Lehmann, Nico Ueberschaar, Carlos Sanchez-Arcos, Kai Uwe Totsche, Georg Pohnert
Summary: Understanding the composition, transport and evolution of dissolved organic matter in groundwater is crucial for its protection and sustainable use. Groundwater metabolomics provides valuable insights into the response of groundwater ecosystems to external impacts and the effects of recharge dynamics.
Article
Environmental Sciences
Thomas Ritschel, Kai Uwe Totsche
Summary: Soil biogeochemistry is interconnected with the redox cycling of iron and manganese, where manganese oxides serve as electron acceptors and might be reduced by organic substances as electron donors. In this study, we investigated the impact of low-molecular-weight organic acids (LMWOA) on the transformation of birnessite. Our findings suggest that the reductive transformation of birnessite into more stable forms of manganese oxides is facilitated by the oxidation of LMWOA, which serves as electron donors. This process plays a critical role in controlling the abundance of LMWOA in natural systems.
Article
Agronomy
Tom Guhra, Arnold Wonneberger, Katharina Stolze, Thomas Ritschel, Kai Uwe Totsche
Summary: This study investigates the role of cutaneous earthworm mucus (CEM) in soil organic-mineral associations and aggregation. The results suggest that pH and concentration affect the adsorption of CEM to goethite. Furthermore, mucus-mineral associations contribute to soil structure and nutrient storage.
JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE
(2023)
Article
Environmental Sciences
Saqr Munassar, Guillaume Monteil, Marko Scholze, Ute Karstens, Christian Roedenbeck, Frank-Thomas Koch, Kai U. Totsche, Christoph Gerbig
Summary: This study analyzes the impact of atmospheric transport on estimating CO2 fluxes over Europe in 2018 using two atmospheric inversion systems. The main focus is to quantify the drivers of spread in CO2 estimates from atmospheric tracer inversions. The results show that differences in the mesoscale transport model contribute the most to the discrepancies in annual budget, followed by far-field contributions and the inversion systems. Varying the mesoscale transport leads to large discrepancies in spatial and temporal patterns, while changing the lateral boundary conditions result in more homogeneous impact. The choice of inversion system and prior error structure also have significant impacts on the CO2 estimates.
ATMOSPHERIC CHEMISTRY AND PHYSICS
(2023)
Article
Soil Science
Martina Herrmann, Katharina Lehmann, Kai Uwe Totsche, Kirsten Kuesel
Summary: The translocation of soil microorganisms across the soil-regolith boundary through seepage plays a vital role in connecting surface and subsurface habitats. This study investigated the factors influencing this translocation and found that the taxonomic identity of bacteria and the seasonal infiltration regime were the main drivers. The results highlight the continuous export of substantial bacterial biomass from soils to subsurface environments and suggest that the introduction of bacterivorous or potential pathogenic taxa from soil to groundwater may be an important factor in groundwater food webs.
SOIL BIOLOGY & BIOCHEMISTRY
(2023)
Article
Environmental Sciences
Saqr Munassar, Christian Rodenbeck, Frank-Thomas Koch, Kai U. Totsche, Michal Galkowski, Sophia Walther, Christoph Gerbig
Summary: Three-hourly net ecosystem exchange (NEE) estimation and uncertainty assessment on the European continent at a spatial scale of 0.25 degrees revealed significant impact from the selection of atmospheric stations, with smaller contributions from biosphere and ocean ensembles. Unprecedented temperature increase and drought events in 2018 and 2019 led to reduced CO2 uptake and weaker sink of CO2 in Europe.
ATMOSPHERIC CHEMISTRY AND PHYSICS
(2022)