期刊
ELIFE
卷 6, 期 -, 页码 -出版社
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.26120
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资金
- Fundacao para a Ciencia e Tecnologia [SRFH/BD/52172/2013]
- FCT Investigator grant [IF/00851/2012/CP0185/CT0004 WP1]
- Marie Curie Career Integration Grant [MCCIG321883/CCC]
- EMBO Installation Grant [IG2778]
- European Research Council Starting Grant [ERC-2014-STG-638917]
- Fundação para a Ciência e a Tecnologia [IF/00851/2012/CP0185/CT0004] Funding Source: FCT
Mitotic chromosome assembly remains a big mystery in biology. Condensin complexes are pivotal for chromosome architecture yet how they shape mitotic chromatin remains unknown. Using acute inactivation approaches and live-cell imaging in Drosophila embryos, we dissect the role of condensin I in the maintenance of mitotic chromosome structure with unprecedented temporal resolution. Removal of condensin I from pre-established chromosomes results in rapid disassembly of centromeric regions while most chromatin mass undergoes hyper-compaction. This is accompanied by drastic changes in the degree of sister chromatid intertwines. While wild-type metaphase chromosomes display residual levels of catenations, upon timely removal of condensin I, chromosomes present high levels of de novo Topoisomerase II (TopoII)-dependent re-entanglements, and complete failure in chromosome segregation. TopoII is thus capable of re-intertwining previously separated DNA molecules and condensin I continuously required to counteract this erroneous activity. We propose that maintenance of chromosome resolution is a highly dynamic bidirectional process.
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