期刊
BIOTECHNOLOGY JOURNAL
卷 12, 期 11, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201700110
关键词
Issatchenkia orientalis; multiple tolerance; fermentation; metabolomics; bioethanol
资金
- National Research Foundation of Korea (NRF) - Korean Ministry of Science, ICT, and Future Planning [2016R1A2B4010842]
- R&D Program of MOTIE/KEIT [10049675]
- National Research Foundation of Korea [2016R1A2B4010842] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Yeast with multiple tolerance onto harsh conditions has a number of advantages for bioethanol production. In this study, an alcohol yeast of Issatchenkia orientalis MTY1 is isolated in a Korean winery and its multiple tolerance against high temperature and acidic conditions is characterized in microaerobic batch cultures and by metabolomic analysis. In a series of batch cultures using 100 g L-1 glucose, I. orientalis MTY1 possesses wider growth ranges at pH 2-8 and 30-45 degrees C than a conventional yeast of Saccharomyces cerevisiae D452-2. Moreover, I. orientalis MTY1 showes higher cell growth and ethanol productivity in the presence of acetic acid or furfural than S. cerevisiae D452-2. I. orientalis MTY1 produces 41.4 g L-1 ethanol with 1.5 g L-1 h(-1) productivity at 42 degrees C and pH 4.2 in the presence of 4 g L-1 acetic acid, whereas a thermo-tolerant yeast of Kluyvermyces marxianus ATCC36907 does not grow. By metabolomics by GC-TOF MS and statistical analysis of 125 metabolite peaks, it is revealed that the thermo-tolerance of I. orientalis MTY1 might be ascribed to higher contents of unsaturated fatty acids, purines and pyrimidines than S. cerevisiae D452-2. Conclusively, I. orientalis MTY1 could be a potent workhorse with multiple tolerance against harsh conditions considered in cellulosic bioethanol production.
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