Article
Medicine, General & Internal
Li Xue, Biao Wang, Jianhong Zhu, Qian He, Fang Huang, Wei Wang, Li Tao, Yan Wang, Nan Xu, Ni Yang, Li Jin, Hua Zhang, Ning Gao, Ke Lei, Yanping Zhang, Chaoliang Xiong, Jing Lei, Ting Zhang, Yan Geng, Ming Li
Summary: This study aimed to investigate the key circRNAs related to rheumatoid arthritis (RA) by identifying differentially expressed circRNAs (DEcircRNAs) in peripheral blood mononuclear cells (PBMCs) from RA patients compared to controls and osteoarthritis (OA) patients. Real-time PCR validation and correlation analysis with laboratory indices were also performed. The functional annotation of host genes of the DEcircRNAs in RA revealed their involvement in metabolic pathways, ECM-receptor interaction, and the PI3K-Akt signalling pathway. These findings contribute to a better understanding of the role of circRNAs in the specific mechanism underlying RA.
ANNALS OF MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Amit Kumar Rai, K. Shanmugha Rajan, Malik Bisserier, Agnieszka Brojakowska, Aimy Sebastian, Angela C. Evans, Matthew A. Coleman, Paul J. Mills, Arsen Arakelyan, Shizuka Uchida, Lahouaria Hadri, David A. Goukassian, Venkata Naga Srikanth Garikipati
Summary: During spaceflight, astronauts experience physical and psychological stressors that can negatively impact their health. Researchers have found that changes in small nucleolar RNA (snoRNA) may be associated with these effects. By studying extracellular vesicles and mononuclear cells in astronauts' peripheral blood, they discovered altered snoRNA expression after spaceflight.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Gwendolyn van Gorkom, Birgit Gijsbers, Erik-Jan Ververs, Ahmed El Molla, Cindy Sarodnik, Celine Riess, Will Wodzig, Gerard Bos, Catharina Van Elssen
Summary: Given the growing interest in ascorbic acid (AA), a method to measure AA levels in the human body is needed. This study presents a method using hydrophilic interaction liquid chromatography (HILIC) to measure AA in peripheral blood mononuclear cells (PBMCs). The method is also applicable to plasma and other leukocyte subsets. The study found that the method is reliable and accurate in measuring AA in PBMCs and plasma.
Article
Health Care Sciences & Services
Mario Giosue Balzanelli, Pietro Distratis, Rita Lazzaro, Ernesto D'Ettorre, Andrea Nico, Francesco Inchingolo, Gianna Dipalma, Diego Tomassone, Emilio Maria Serlenga, Giancarlo Dalagni, Andrea Ballini, Kieu Cao Diem Nguyen, Ciro Gargiulo Isacco
Summary: The COVID-19 pandemic continues to pose a severe threat, with over 280,000 deaths worldwide. There are no specific treatments or conclusive vaccine available yet. Efforts to define therapeutic protocols are ongoing, but the disease remains critical with a high mortality rate. Treatment with plasma from survivors is being explored as a potential effective solution.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Oncology
Zhuyan Jiang, Zhiting Zhong, Qingqing Miao, Yue Zhang, Bing Ni, Mengjie Zhang, Jun Tang
Summary: High-throughput RNA sequencing revealed differential expression of circRNAs in RA patients compared to HCs, with circPTPN22 identified as a common differentially expressed gene in RA and SLE. The expression of circPTPN22 demonstrated high diagnostic value in distinguishing RA patients from HCs and SLE patients. Furthermore, circPTPN22 levels were positively correlated with various RA-related factors in PBMCs.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Biology
Shanwen Chen, Yunfan Jin, Siqi Wang, Shaozhen Xing, Yingchao Wu, Yuhuan Tao, Yongchen Ma, Shuai Zuo, Xiaofan Liu, Yichen Hu, Hongyan Chen, Yuandeng Luo, Feng Xia, Chuanming Xie, Jianhua Yin, Xin Wang, Zhihua Liu, Ning Zhang, Zhenjiang Zech Xu, Zhi John Lu, Pengyuan Wang
Summary: The utility of cell-free nucleic acids in monitoring cancer has been recognized. By profiling cfRNAs in plasma samples, human and microbe-derived plasma cfRNAs were found to have diagnostic value for cancer detection. Adding microbial features improved the accuracy of tumor classification.
Article
Biochemistry & Molecular Biology
Manuel Sanchez, Lorena Suarez, Gabriela Banda, Eva Barreiro-Alonso, Ignacio Rodriguez-Una, Jose Manuel Rubin, Begona Cantabrana
Summary: This study analyzed the age-dependent variations in polyamine content in peripheral blood cells and plasma, and found that the levels of polyamines decreased with age. Specifically, the 60-70-year-old age group showed significant decreases in polyamine content in blood cells and plasma. Further studies should investigate whether supplementation of polyamines can restore the decreased levels and provide long-term biological benefits.
Article
Oncology
Qing Luo, Xue Li, Biqi Fu, Lu Zhang, Le Fang, Cheng Qing, Yang Guo, Zikun Huang, Junming Li
Summary: The study identified dysregulated circRNAs in peripheral blood of patients with SLE, with hsa_circ_0082688-hsa_circ_0082689 combination showing potential diagnostic value.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Ivan Yu Kompaneets, Evgeny A. Ermakov, Valentina N. Buneva, Georgy A. Nevinsky
Summary: Human milk contains various components that promote newborn growth and provide immune protection. These components include antibodies that hydrolyze peptides, proteins, DNAs, RNAs, and oligosaccharides. Non-coding microRNAs in human milk have different biological functions, and IgAs antibodies in milk have higher miRNA-hydrolyzing activities compared to IgG antibodies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Jerome Salignon, Omid R. Faridani, Tasso Miliotis, Georges E. Janssens, Ping Chen, Bader Zarrouki, Rickard Sandberg, Pia Davidsson, Christian G. Riedel
Summary: By analyzing proteins and small RNAs in human blood plasma samples, it was found that combining protein and miRNA data can result in more accurate age-predictive models, possibly capturing a broader range of age-related physiological changes.
Article
Orthopedics
Yufeng Chen, Xianghe Xu, Xuegang Li, Junlong Zhong, Biao Wu, Jie Shang, Ning Jiang, Bin Wang, Shuping Zhong, Huading Lu
Summary: The study found that the expression of hsa_circ_0140271 in female RA was significantly higher than in female HC, and it was positively correlated with antistreptolysin (ASO). The ROC curve indicated that hsa_circ_0140271 could distinguish female RA from female HC, ankylosing spondylitis (AS) or osteoarthritis (OA) patients. Combined diagnosis of Anti-CCP + hsa_circ_0140271 could improve diagnostic accuracy. KEGG pathway enrichment analysis showed hsa_circ_0140271 may act as a microRNA sponge and participate in fatty acid metabolism pathways.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2021)
Article
Multidisciplinary Sciences
Ruijuan Guan, Suonan Angxiu, Ling Li, Zefeng Kang, Xin Yan
Summary: This study identified differentially expressed circRNAs (DEcircRNAs) in peripheral blood samples from patients with glaucoma through transcriptome sequencing, suggesting that DEcircRNAs are involved in the pathogenesis of glaucoma and may serve as potential biomarkers and therapeutic targets.
SCIENTIFIC REPORTS
(2023)
Article
Food Science & Technology
Rosalind Davies, Janna A. van Diepen, Lauren R. Brink, Sabina Bijlsma, Karen-Anne McVey Neufeld, John F. Cryan, Siobhain M. O'Mahony, Ivana Bobeldijk, Gabriele Gross
Summary: This study investigates the bioavailability of bovine milk fat globule membrane (bMFGM) and its effect on lipid composition in the plasma and brain of rodents. The results show that a bMFGM-enriched diet can alter plasma phospholipid composition, predominantly increasing sphingomyelin levels. However, no significant changes were observed in the brain lipidome. These findings suggest that the changes induced by bMFGM may contribute to the beneficial effects on neurodevelopment during early life.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Immunology
Xi Zhou, Xuan Lu, Jing He, Ziwei Xu, Qian Li, Pian Ye, Zhaodong Zhong, Wei Shi, Han Yan, Yong You, Yu Hu, Huafang Wang
Summary: This study investigates the application of intracellular and extracellular Epstein-Barr virus (EBV) DNA in allogeneic hematopoietic stem cell transplantation (allo-HSCT). By monitoring the EBV DNA load and qualitative detection in the blood, the risk of EBV-related posttransplant lymphoproliferative disorders (EBV-PTLD) can be more accurately differentiated, and the transplant outcomes can be stratified based on the risk of EBV DNA level. The study also reveals that patients with intermediate-level of EBV DNA load have better overall survival and lower transplant-related mortality. The high-level of EBV DNA load is associated with poor T cell reconstitution and abnormal increase in IL-10 level. Furthermore, patients with peak EBV DNA load in peripheral blood mononuclear cells (PBMCs) during 3-12 months after transplantation are more prone to chronic graft versus host disease (GVHD). In conclusion, EBV DNA plays a significant role in predicting the risk of EBV-PTLD and GVHD, and intermediate-level of EBV DNA may contribute to favorable prognosis of allo-HSCT.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biology
Steffen M. Recktenwald, Greta Simionato, Marcelle G. M. Lopes, Fabia Gamboni, Monika Dzieciatkowska, Patrick Meybohm, Kai Zacharowski, Andreas von Knethen, Christian Wagner, Lars Kaestner, Angelo D'Alessandro, Stephan Quint
Summary: The flow properties and shape of red blood cells in COVID-19 patients are significantly impaired, but these changes are reversible when suspended in healthy plasma. The study also reveals a new role of red blood cells in maintaining plasma equilibrium. These findings provide a framework for further investigations into the treatment of COVID-19 and other infectious diseases.
Article
Food Science & Technology
Sergey E. Sedykh, Lada V. Purvinish, Evgeniya E. Burkova, Pavel S. Dmitrenok, Valentin V. Vlassov, Elena I. Ryabchikova, Georgy A. Nevinsky
Summary: The study presents the method and results of describing exosomes in horse milk for the first time, obtaining vesicle-rich samples using standard separation techniques, conducting various analytical methods, and confirming the presence of specific proteins in the exosomes.
INTERNATIONAL DAIRY JOURNAL
(2021)
Editorial Material
Chemistry, Multidisciplinary
Elena Ryabchikova
Article
Biology
Maya Dymova, Maria Dmitrieva, Elena Kuligina, Vladimir Richter, Sergey Savinov, Ivan Shchudlo, Tatiana Sycheva, Iuliia Taskaeva, Sergey Taskaev
Summary: In boron neutron capture therapy, the total absorbed dose is the sum of four dose components with different relative biological effectiveness: boron dose, nitrogen dose, fast neutron dose and gamma-ray dose. A new method for measuring the first three doses is presented in this study, along with details and results of employing the proposed method.
RADIATION RESEARCH
(2021)
Article
Cell Biology
Natalia S. Vasileva, Elena Kuligina, Maya A. Dymova, Yulya Savinovskaya, Nikita D. Zinchenko, Alisa B. Ageenko, Sergey Mishinov, Anton S. Dome, Grigory A. Stepanov, Vladimir A. Richter, Dmitry Semenov
Summary: Glioma, the most common and heterogeneous primary brain tumor, requires the development of new preclinical models. Comparing adherent and neurosphere cell cultures, this study revealed the activation of specific transcription factors during neurosphere formation and identified common signaling feedback regulators in gliomas for potential therapeutic strategies against invasion and progression.
Article
Biochemistry & Molecular Biology
Sergey E. Sedykh, Lada V. Purvinsh, Evgeniya E. Burkova, Pavel S. Dmitrenok, Elena I. Ryabchikova, Georgy A. Nevinsky
Summary: This study purified highly purified vesicle preparations from horse milk samples using different techniques, and analyzed their composition and characteristics. The results revealed the uneven distribution of tetraspanins and the presence of peptides on the surface of horse milk exosomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Vera Morozova, Yuliya Kozlova, Ghadeer Jdeed, Artem Tikunov, Tatyana Ushakova, Alevtina Bardasheva, Elena Zhirakovskaia, Yuliya Poletaeva, Elena Ryabchikova, Nina V. Tikunova
Summary: Aeromonas popoffii is an environmental species that can cause urinary tract infections. The discovery of the A. popoffii bacteriophage AerP_220 in river water suggests a potential new species within the Autographiviridae family.
Article
Chemistry, Multidisciplinary
Anna V. Epanchintseva, Julia E. Poletaeva, Anton S. Dome, Ilya S. Dovydenko, Inna A. Pyshnaya, Elena I. Ryabchikova
Summary: Small interfering RNAs (siRNAs) are powerful tools for suppressing protein synthesis in the cell, but they face challenges such as low resistance to nucleases and inability to enter target cells. In this study, researchers designed multilayer nanoconstructs (MLNCs) with gold nanoparticle cores carrying chemically modified siRNAs. By introducing chemical modifications, the efficiency of siRNA adsorption onto the gold core was increased. The stability of modified siRNA on the gold core was also tested, and MLNCs with the most effective siRNA cargo were selected. These MLNCs showed increased biological activity compared to control MLNCs.
Article
Cell Biology
Ekaterina Patrakova, Mikhail Biryukov, Olga Troitskaya, Pavel Gugin, Elena Milakhina, Dmitriy Semenov, Julia Poletaeva, Elena Ryabchikova, Diana Novak, Nadezhda Kryachkova, Alina Polyakova, Maria Zhilnikova, Dmitriy Zakrevsky, Irina Schweigert, Olga Koval
Summary: Cold atmospheric plasma (CAP) is a widely studied approach for treating malignant neoplasms. This study determined the optimal discharge regime for direct CAP treatment of cancer cells and found that CAP weakly activated pro-survival autophagy in irradiated cells. The addition of the autophagy inhibitor chloroquine (CQ) promoted CAP-dependent cell death.
Article
Biochemistry & Molecular Biology
Anastasiya V. Tupitsyna, Alina E. Grigorieva, Svetlana E. Soboleva, Nadezhda A. Maltseva, Sergey E. Sedykh, Julia Poletaeva, Pavel S. Dmitrenok, Elena I. Ryabchikova, Georgy A. Nevinsky
Summary: We have studied the possibility of obtaining appropriate preparations of EVs from whole-body extract of holothuria Eupentacta fraudatrix using centrifugation and ultracentrifugation. However, the preparations were heavily polluted and subsequent purification didn't increase vesicle concentration to a necessary level. To confirm the presence of EVs, including exosomes, in holothurian cells, we used transmission electron microscopy and found late endosomes in the coelom epithelium and parenchyma cells of visceral organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alesya Fokina, Yulia Poletaeva, Svetlana Dukova, Kristina Klabenkova, Zinaida Radkova, Anastasia Bakulina, Timofei Zatsepin, Elena Ryabchikova, Dmitry Stetsenko
Summary: A template-assisted assembly approach to a C24 fullerene-like double-stranded DNA polyhedral shell is proposed. The assembly employed a supramolecular oligonucleotide dendrimer as a 3D template that was obtained via the hybridization of siRNA strands and a single-stranded DNA oligonucleotide joined to three- or four-way branched junctions. The article discusses the use of a photolabile protecting group to prevent premature auto-hybridization of the self-complementary oligonucleotides, as well as the incorporation of cleavable linkers to guide the post-assembly disconnection of the shell. The study also explores the modification of siRNA strands to enhance enzymatic stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Alina E. Grigor'eva, Alevtina V. Bardasheva, Elena S. Ryabova, Anastasiya V. Tupitsyna, Danila A. Zadvornykh, Lyudmila S. Koroleva, Vladimir N. Silnikov, Nina V. Tikunova, Elena I. Ryabchikova
Summary: Understanding the relationship between the effects of antibacterial compounds and their chemical structure is crucial for the purposeful development of synthetic antibacterial compounds. In this study, we examined the ultrastructural changes in S. aureus cells caused by the polycationic amphiphile DL(4)12, ciprofloxacin, and their hybrid DL(5)Cip6. Our findings revealed that DL(4)12 damaged the bacterial cell wall and disrupted the cytoplasm, while ciprofloxacin altered the cell membrane and cytoplasm and inhibited bacterial division. The ultrastructural characteristics of S. aureus cells damaged by the hybrid differed from those under ciprofloxacin or DL(4)12 action. Furthermore, we observed clear differences in the effects of these compounds on the morphology of S. aureus biofilm.
Article
Biochemistry & Molecular Biology
Mikhail Biryukov, Dmitriy Semenov, Nadezhda Kryachkova, Alina Polyakova, Ekaterina Patrakova, Olga Troitskaya, Elena Milakhina, Julia Poletaeva, Pavel Gugin, Elena Ryabchikova, Dmitriy Zakrevsky, Irina Schweigert, Olga Koval, Pankaj Attri, Jamoliddin Razzokov, Maksudbek Yusupov
Summary: This study uncovers the molecular mechanisms behind the selective death of tumor cells under cold atmospheric plasma (CAP) exposure. The response to CAP treatment was analyzed in lung adenocarcinoma cells and lung-fibroblast cells, revealing common trends in both cell lines but with variable amplitude in the tumor cells. CAP treatment resulted in DNA damage, cell-cycle arrest, and ER lumens dysfunction in the tumor cells. These findings suggest that nuclear stress and ER stress contribute to the differential sensitivity of tumor and healthy cells to CAP exposure.
Article
Biochemistry & Molecular Biology
Yulya I. I. Savinovskaya, Anna A. A. Nushtaeva, Anna V. V. Savelyeva, Vitaliy V. V. Morozov, Elena I. I. Ryabchikova, Elena V. V. Kuligina, Vladimir A. A. Richter, Dmitriy V. V. Semenov
Summary: Extracellular vesicles (EVs) are heterogeneous in size and composition, and changes in their RNA sets can be used for diagnostic and therapeutic approaches. This study obtained EVs from the blood of healthy donors and analyzed their RNA composition. The results suggest that EVs can trigger the NF-kB signaling pathway in lung adenocarcinoma cells, leading to the transcription of genes involved in cytokines, adhesion molecules, cell cycle regulation, and cell survival.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maya A. Dymova, Natalia S. Vasileva, Elena Kuligina, Yulya Savinovskaya, Nikita D. Zinchenko, Alisa B. Ageenko, Sergey Mishinov, Grigory A. Stepanov, Vladimir A. Richter, Dmitry Semenov
Summary: The formation of neurospheres in GBM cells is accompanied by changes in the expression of miRNAs and their target mRNAs. Upregulated miRNAs are associated with decreased levels of their target mRNAs, while downregulated miRNAs are associated with increased levels of their target mRNAs. These miRNAs and their target mRNAs may play important roles in the regulation of malignant growth and invasion in GBM.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)