Article
Public, Environmental & Occupational Health
Jedas Veronica Daag, Michelle Ylade, Ramesh Jadi, Cameron Adams, Anna Maureen Cuachin, Riacarl Alpay, Emma Teresa Carmela Aportadera, In-Kyu Yoon, Aravinda M. de Silva, Anna Lena Lopez, Jacqueline Deen
Summary: The use of dried blood spots for detecting dengue antibodies showed good performance in sensitivity, specificity, positive predictive value, and negative predictive value compared to standard serum collection methods, making it a practical option for large-scale serosurveys in dengue populations.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Chemistry, Analytical
Yojana J. P. Carreon, Mary Luz Gomez-Lopez, Orlando Diaz-Hernandez, Pamela Vazquez-Vergara, Rosario E. Moctezuma, Jose M. Saniger, Jorge Gonzalez-Gutierrez
Summary: The study demonstrates that high concentrations of unfolded conformational BSA result in an eye-like pattern, while low concentrations lead to the formation of amorphous aggregates in dried droplets.
Article
Immunology
Fernando Echegaray, Peter Laing, Samantha Hernandez, Sully Marquez, Amanda Harris, Ian Laing, Adam Chambers, Neil McLennan, Victor A. Sugiharto, Hua-Wei Chen, Sandra Vivero Villagran, Abigail Collingwood, Magelda Montoya, Fausto Bustos Carrillo, Mark P. Simons, Philip J. Cooper, Andrea Lopez, Gabriel Trueba, Joseph Eisenberg, Shuenn-Jue Wu, William Messer, Eva Harris, Josefina Coloma, Leah C. Katzelnick
Summary: The study showed that both existing and newly developed anti-flavivirus rapid diagnostic tests can effectively screen for prior DENV infection to reduce the risk of severe dengue induced by vaccination, and quantitative evaluation can further optimize the rapid diagnostic tests for this purpose.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Aidan M. Nikiforuk, Brynn McMillan, Sofia R. Bartlett, Ana Citlali Marquez, Tamara Pidduck, Jesse Kustra, David M. Goldfarb, Vilte Barakauskas, Graham Sinclair, David M. Patrick, Manish Sadarangani, Gina S. Ogilvie, Mel Krajden, Muhammad Morshed, Inna Sekirov, Agatha N. Jassem
Summary: Quantitative multiplex anti-IgG assay tested on finger prick capillary dried blood spot (DBS) samples is accurate in detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies after infection or vaccination. It has high sensitivity and specificity in unvaccinated individuals and even higher sensitivity in COVID-19 vaccine recipients. Modeling analysis showed that DBS testing has a high positive predictive value in a population with high seroprevalence.
MICROBIOLOGY SPECTRUM
(2022)
Article
Cell Biology
Andreana N. Holowatyj, Biljana Gigic, Christy A. Warby, Jennifer Ose, Tengda Lin, Petra Schrotz-King, Cornelia M. Ulrich, Jamie J. Bernard
Summary: The study found that human serum has the potential to stimulate growth in non-tumorigenic cells, but circulating levels of FGF2 were not significantly correlated. The transforming effects of human serum may require FGF2 activation of FGFR1.
Article
Engineering, Chemical
Monserrat Ancheyta-Palacios, Iris G. G. Velasco-Teran, Yojana J. P. Carreon, Jorge Gonzalez-Gutierrez
Summary: Hyperlipidemia is the elevated concentration of lipids in the blood, which increases the risk of circulatory system complications. This study investigates the formation of patterns in dried blood droplets with varying concentrations of ultrapure water. By calculating the entropy of the GLCM and analyzing ROC curves, solutions with 4% and 12% hematocrit exhibit over 95% accuracy in differentiating high lipid concentrations. These findings show promise for the development of diagnostic methods based on diluted blood coatings.
Article
Environmental Sciences
Tran Thanh Tam Toan, Do Mai Nguyen, Doan Manh Dung, Dang Thi Ngoc Hoa, Le Thi Thanh Nhi, Nguyen Mau Thanh, Nguyen Nho Dung, Yasser Vasseghian, Nasrin Golzadeh
Summary: In this study, RGO was synthesized using AA as a reducing agent and modified on GCE to create RGO/GCE for the detection of TB in urine samples. Voltammetric analysis, specifically DP-ASV, demonstrated higher sensitivity and selectivity compared to spectrophotometric methods in detecting TB. The RGO/GCE showed excellent repeatability, reproducibility, and long-term stability, making it more effective in detecting TB in urine samples than bare GCE.
Article
Nanoscience & Nanotechnology
Nan-Fu Chiu, Ming-Jung Tai, Devi Taufiq Nurrohman, Ting-Li Lin, Ying-Hao Wang, Chen-Yu Chen
Summary: A highly sensitive SPR biosensor using carboxyl-MoS2 film was developed for evaluating PAPP-A2 levels in maternal serum samples for Down's syndrome screening. The technology showed acceptable accuracy and recovery rates, suggesting potential advantages over conventional ELISA.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Multidisciplinary Sciences
Toshifumi Imagawa, Masahiko Ito, Mami Matsuda, Kenji Nakashima, Yuhei Tokunaga, Isao Ohta, Tian-Cheng Li, Ryosuke Suzuki, Tetsuro Suzuki
Summary: This study developed a novel vaccine platform based on tetravalent dengue virus-like particles, which successfully induced neutralizing activity against all four DENV serotypes in mice, showing promise for future vaccine development against dengue and other emerging pandemics.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Braulio M. Valencia, Ponsuge C. Sigera, Praveen Weeratunga, Nicodemus Tedla, Deepika Fernando, Senaka Rajapakse, Andrew R. Lloyd, Chaturaka Rodrigo
Summary: A previous infection with the Zika virus may increase the severity of a subsequent dengue virus infection. A study in Sri Lanka found that individuals with prior exposure to both dengue and Zika, or to dengue alone, were at a higher risk of severe dengue symptoms. However, the effect of prior Zika exposure alone could not be determined due to a small number of primary dengue infections with prior Zika exposure.
SCIENTIFIC REPORTS
(2022)
Article
Virology
Bastian Fischer, Christoph Lichtenberg, Lisa Muller, Jorg Timm, Johannes Fischer, Cornelius Knabbe
Summary: The determination of anti-SARS-CoV-2 neutralizing antibodies is crucial for evaluating the effectiveness of convalescent plasma therapy. A novel ELISA-based surrogate virus neutralization test (sVNT) is shown to be suitable for easily measuring the inhibition-capability of NAbs in plasma. By comparing and combining results from different assays, high-titer plasma samples can be reliably detected while reducing the risk of misidentification.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Food Science & Technology
Florence E. Buytaers, Bavo Verhaegen, Mathieu Gand, Jolien D'aes, Kevin Vanneste, Nancy H. C. Roosens, Kathleen Marchal, Sarah Denayer, Sigrid C. J. De Keersmaecker
Summary: This proof-of-concept study explores the feasibility of using metagenomics to obtain whole genome sequences of norovirus from contaminated food and perform strain level characterization, as well as relate to human cases. The study compares different sequencing methods and demonstrates that shotgun metagenomics is effective for phylogenetic analysis while hybrid capture is suitable for lower contamination. The study also shows the potential of shotgun metagenomics to detect multiple viruses in food samples.
Article
Biochemical Research Methods
Kristina Allgoewer, Shuvadeep Maity, Alice Zhao, Lauren Lashua, Moti Ramgopal, Beni N. Balkaran, Liyun Liu, Savita Purushwani, Maria T. Arevalo, Ted M. Ross, Hyungwon Choi, Elodie Ghedin, Christine Vogel
Summary: Distinguishing between Zika and dengue virus infections is crucial for accurate treatment. Using next-generation proteomics, the study identified 13 differentially expressed proteins related to infection, wound healing, pregnancy, and brain function. Three of these proteins showed potential for predicting Zika virus infection, and temporal changes in protein signatures can help differentiate diagnoses and indicate past infections.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Chemistry, Multidisciplinary
Ghada AlMisned, Tariq Al-Abdullah, Fatai Liadi, Adil Hawsawi
Summary: Prompt gamma-ray neutron activation analysis (PGNAA) is a non-destructive method for determining element concentrations in natural materials. This study improved the detection sensitivity by optimizing the setup.
APPLIED SCIENCES-BASEL
(2022)
Article
Biology
Afrina Andriani Sebayang, Hilda Fahlena, Vizda Anam, Damian Knopoff, Nico Stollenwerk, Maira Aguiar, Edy Soewono
Summary: Dengue fever is a major global public health concern with over one-third of the world's population at risk of infection. Disease severity is influenced by the individual's immunological status before natural infection and the different serotypes of the virus. A secondary dengue infection with a different serotype increases the risk of severe disease. Developing a modeling framework to describe the immune responses to dengue virus can provide insights into the immunopathogenesis and help evaluate the impact of dengue vaccines.
Article
Biochemistry & Molecular Biology
Jose Abrahan Ramirez-Pool, Beatriz Xoconostle-Cazares, Berenice Calderon-Perez, Enrique Ibarra-Laclette, Emanuel Villafan, Rosalia Lira-Carmona, Roberto Ruiz-Medrano
Summary: This study investigated the relationship between symptoms caused by Citrus tristeza virus (CTV) and virus strains through transcriptomic analysis. It identified polymorphisms in the coat protein encoding gene that distinguish between mild and severe strains. Additionally, the study found the presence of other viroids in trees with severe symptoms, providing insights into citrus-virus interaction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Youyi Fong, Jessica Markby, Mauro Andreotti, Ingrid Beck, Thomas Bourlet, Don Brambilla, Lisa Frenkel, Rosalia Lira, Julie A. E. Nelson, Georgios Pollakis, Sandrine Reigadas, Douglas Richman, Souleymane Sawadogo, Laura Waters, Chunfu Yang, Clement Zeh, Meg Doherty, Lara Vojnov
Summary: The study reviewed 23 studies comparing the diagnostic accuracy of dried plasma spots and liquid plasma, finding that dried plasma spots had sensitivity and specificity greater than 90% and misclassification rates below 10% across all treatment failure thresholds. Dried plasma spots performed well at treatment failure thresholds.
JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
(2022)
Letter
Pediatrics
Cristina de Jesus Herrera-Castillo, Angelica Maldonado-Rodriguez, Othon Rojas-Montes, Ana Maria Cevallos, Jose Guillermo Vazquez-Rosales, Rosalia Lira
WORLD JOURNAL OF PEDIATRICS
(2022)
Article
Agriculture, Dairy & Animal Science
Rocio Gomez, Rocio M. Neri-Bazan, Araceli Posadas-Mondragon, Pablo A. Vizcaino-Dorado, Jonathan J. Magana, Jose Leopoldo Aguilar-Faisal
Summary: In this study, 15 hypervariable molecular markers were selected and analyzed in 210 captive dolphins to evaluate their usefulness. The proposed set of markers can help maintain genetic diversity and can be applied to other endangered species in conservation programs.
Article
Chemistry, Multidisciplinary
Angelica Perez-Juarez, Jose Leopoldo Aguilar-Faisal, Araceli Posadas-Mondragon, Jose Angel Santiago-Cruz, Cornelio Barrientos-Alvarado, Maria Angelica Mojica-Villegas, German Alberto Chamorro-Cevallos, Jose A. Morales-Gonzalez
Summary: This study evaluated the protective properties of Spirulina against ethanol-induced toxicity. The administration of Spirulina extract restored the biochemical and histopathological changes caused by ethanol, and regulated antioxidant enzyme levels.
APPLIED SCIENCES-BASEL
(2022)
Review
Plant Sciences
Nancy Vargas-Mendoza, Eduardo Madrigal-Santillan, Isela Alvarez-Gonzalez, Eduardo Madrigal-Bujaidar, Liliana Anguiano-Robledo, Jose Leopoldo Aguilar-Faisal, Mauricio Morales-Martinez, Luis Delgado-Olivares, Elda Victoria Rodriguez-Negrete, Angel Morales-Gonzalez, Jose A. Morales-Gonzalez
Summary: Muscle health is crucial for human mobility and can be affected by various factors such as aging, oxidative stress, malnutrition, and lack of exercise. Nutritional factors play a vital role in maintaining the balance necessary for muscle function and recovery. Phytochemicals, particularly curcumin and sulforaphane, have shown positive effects on muscle function, recovery, and prevention of muscle atrophy. Future research may explore novel compounds as potential treatments for muscle-related medical conditions.
Article
Infectious Diseases
Lucero Rojas-Luna, Araceli Posadas-Modragon, Amanda M. Avila-Trejo, Veronica Alcantara-Farfan, Lorena Rodriguez-Paez, Jose Angel Santiago-Cruz, Marvin O. Pastor-Alonso, J. Leopoldo Aguilar-Faisal
Summary: This study found that N-& omega;-chloroacetyl-L-ornithine (NCAO) inhibits CHIKV replication by reducing the synthesis of intracellular polyamines, leading to a decrease in the production of infectious viral particles.
Article
Multidisciplinary Sciences
Diana Angelica Tapia-Sidas, Brenda Yazmin Vargas-Hernandez, Jose Abrahan Ramirez-Pool, Leandro Alberto Nunez-Munoz, Berenice Calderon-Perez, Rogelio Gonzalez-Gonzalez, Luis Gabriel Brieba, Rosalia Lira-Carmona, Eduardo Ferat-Osorio, Constantino Lopez-Macias, Roberto Ruiz-Medrano, Beatriz Xoconostle-Cazares
Summary: The aim of this study was to develop and describe molecular detection systems for SARS-CoV-2 based on RT-LAMP. Recombinant DNA polymerase and reverse transcriptase were purified and used to set up fluorometric real time and colorimetric end-point RT-LAMP assays. The developed RT-LAMP diagnostic tests allowed a sensitive and specific detection of the nucleocapsid gene of SARS-CoV-2.
Article
Immunology
Ricardo Serna-Munoz, Alejandra Hernandez-Teran, Maribel Soto-Nava, Daniela Tapia-Trejo, Santiago Avila-Rios, Fidencio Mejia-Nepomuceno, Emma Garcia, Manuel Castillejos-Lopez, Anjarath Lorena Higuera-Iglesias, Arnoldo Aquino-Galvez, Ireri Thirion-Romero, Rogelio Perez-Padilla, Jose Leopoldo Aguilar-Faisal, Joel Armando Vazquez-Perez
Summary: The study aims to characterize the primary humoral immune host response in the early stages of SARS-CoV-2 infection and investigate its association with neutralizing antibodies, antibody levels, viral lineage, and clinical outcomes. The results suggest that developing an early and robust neutralizing response may increase survival probability in critical patients.
Article
Virology
Francisca Sosa-Jurado, Roxana Palencia-Lara, Cinthia Xicotencatl-Grijalva, Maribel Bernal-Soto, Alvaro Montiel-Jarquin, Yolanda Ibarra-Pichardo, Nora Hilda Rosas-Murrieta, Rosalia Lira, Paulina Cortes-Hernandez, Gerardo Santos-Lopez
Summary: To reduce the risk of virus transmission through blood transfusion, individual donor nucleic acid testing (ID-NAT) has been used in Puebla, Mexico. The data of 106,125 blood donors were analyzed to calculate the residual risk (RR) values for different viruses. ID-NAT has successfully increased the safety of blood reserves for HIV and HCV, but more research is needed to determine why the residual risk of HBV did not decrease as much. Therefore, ID-NAT is an important complementary tool that should be implemented in blood donor screening.
Review
Biochemistry & Molecular Biology
Amanda M. Avila-Trejo, Lorena I. Rodriguez-Paez, Veronica Alcantara-Farfan, J. Leopoldo Aguilar-Faisal, Toshio Hattori
Summary: Chronic cases of chikungunya fever pose a public health problem in countries where the virus is present. The disease can persist for years, causing debilitating pain and bone erosion. The mechanisms behind tissue damage and the development of different conditions are not fully understood. This review aims to analyze the molecular and cellular factors that contribute to tissue damage in various infectious conditions caused by the chikungunya virus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Julio Rodrigo Escartin-Gutierrez, Mariana Ponce-Figueroa, Miguel Angel Torres-Vega, Leopoldo Aguilar-Faisal, Paula Figueroa-Arredondo
Summary: This study investigates the role of VCC in activating the macrophage lineage and the mechanism of IL-1 beta production induced by VCC in human macrophages. The results suggest that VCC stimulates the MAPK signaling pathway and activates transcriptional regulators, leading to a pro-inflammatory response. The findings support the involvement of the monomeric soluble form of VCC in modulating the innate immune response and releasing IL-1 beta through the NLRP3 inflammasome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Luis Manuel Garcia-Feria, Leopoldo Aguilar-Faisal, Rosalia Pastor-Nieto, Juan Carlos Serio-Silva
Summary: The health of the gastrointestinal system is affected by changes in the microbiota and the host's adaptability to different habitat conditions. A study on black howler monkeys found that microbial diversity was lower in monkeys from flooded areas compared to non-flooded areas. The composition of the plant community and the fragmentation of the landscape can impact the intestinal microbial community of howler monkeys.
ACTA BIOLOGICA COLOMBIANA
(2023)
