News Item
Biochemistry & Molecular Biology
Kanishk Jain, Brian D. Strahl
Summary: High-throughput biochemical and biological analyses of disease-associated histone mutations reveal key residues in globular cores that affect chromatin remodeling, nucleosome stability, and stem cell pluripotency.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Nesreen Elathram, Bryce E. Ackermann, Evan T. Clark, Shelby R. Dunn, Galia T. Debelouchina
Summary: In the nucleus, silent genes are segregated into heterochromatin compartments, consisting of nucleosomes with H3 Lys9 trimethylation and HP1 alpha protein. Phosphorylated HP1 alpha protein can form liquid droplets and potentially organize heterochromatin through phase separation. Our study reveals that phosphorylated human HP1 alpha does not cause major rearrangements to the nucleosome core, but specifically interacts with methylated H3 tails and slows the dynamics of H4 tails.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Pathology
Hrishikesh Sathyamoorthy, Hanya Mahmood, Amir Zaki Abdullah Zubir, Paul Hankinson, Syed Ali Khurram
Summary: Oral epithelial dysplasia (OED) is a condition that can be diagnosed and graded using histological features. This study explores the importance of mitotic counting and PHH3 staining in OED. The researchers found that mitosis count increased with grade severity, and differences in mitosis type and location were observed among different grades. When combined with histological grading, mitotic number, type, and location were better predictors of transformation and recurrence than conventional grading.
Article
Biology
Sona Legartova, Gabriela Lochmanova, Eva Bartova
Summary: The study revealed differences in the levels and distribution of phosphorylated histone H1 and H3K9me3 during mitotic phases, and identified ESC-specific phosphorylation sites in a linker histone.
Article
Multidisciplinary Sciences
Akira Takazawa, Yasuo Yoshimura, Masanori Okamoto, Atsushi Tanaka, Munehisa Kito, Kaoru Aoki, Takeshi Uehara, Jun Takahashi, Hiroyuki Kato, Jun Nakayama
Summary: This study aimed to assess PHH3 immunohistochemistry as a potential biomarker for predicting the prognosis of MLS. It was found that the PHH3 index and proportion of round cell component were significant factors in determining the prognosis of MLS.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Yin Li, Qinyi Jiang, Xiaohua Zhou, Mengyuan Wu, Jian Chen, Hao Liu, Siming Dai, Ziyang Zheng, Xuan Zhao, Chenxi Zhang, Zhuoying Shi, Haitao Zhang, Jinyu Gu, Zhenfei Huang, Guoyong Yin, Shujie Zhao
Summary: This study investigated the predictive value of neutrophil extracellular traps (NETs) in postoperative deep venous thrombosis (DVT) formation and developed a predictive model based on clinical admission information. The results showed that higher body mass index (BMI), neutrophil levels, D-dimer, and citrullinated histone H3 (CitH3) were independent risk factors for postoperative DVT. The study provides new insights for the prediction and prevention of DVT in patients with lumbar fractures.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Pathology
Chaohui Lisa Zhao, Bahram Dabiri, Iman Hanna, Lili Lee, Xiaofei Zhang, Zarrin Hossein-Zadeh, Cao Wenqing, John Allendorf, Alex Pipas Rodriguez, Katherine Weng, Solomon Turunbedu, Adrienne Boyd, Mala Gupta
Summary: Surgery is the only known cure for sporadic pancreatic neuroendocrine tumors (PNETs). Predicting the biological aggressiveness of PNETs based on endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) has a significant impact on clinical management. Ki-67 and phosphorylated histone H3 (PHH3) can help predict the tumor's aggressiveness, while BCL-2 may contribute to tumorigenesis and neuroendocrine cell differentiation.
ANNALS OF DIAGNOSTIC PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Karin Johanna Ferrari, Simona Amato, Roberta Noberini, Cecilia Toscani, Daniel Fernandez-Perez, Alessandra Rossi, Pasquale Conforti, Marika Zanotti, Tiziana Bonaldi, Simone Tamburri, Diego Pasini
Summary: The study found extensive cleavage of histone H3 N-terminal tails in differentiated cells of mouse intestinal epithelium, demonstrating that intestinal H3 clipping is the result of multiple proteolytic activities including Trypsins and Cathepsin L. These proteolytic activities are differentially affected by the PTM pattern of histone H3 tails.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Mariola Ferreras-Gutierrez, Belen Chaves-Arquero, Amaia Gonzalez-Magana, Nekane Merino, Ignacio Amusategui-Mateu, Sonia Huecas, Francisco J. Medrano, Francisco J. Blanco
Summary: Proteins of the ING family regulate chromatin transcription by recruiting remodeling complexes to sites with H3K4me3 modification. ING3, an oncoprotein, facilitates histone acetylation and forms homodimers with an antiparallel coiled-coil fold. The crystal structure of ING3's PHD domain reveals its interaction with H3K4me3 and the impact of mutations on histone recognition.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Cell Biology
Asmaa Ibrahim, Michael S. Toss, Shorouk Makhlouf, Islam M. Miligy, Fayyaz Minhas, Emad A. Rakha
Summary: Mitotic count in breast cancer is a significant prognostic marker, but manual counting of mitotic figures by pathologists has variation. This study developed a new technique using PHH3-H&E staining to improve reproducibility and accuracy in scoring mitotic figures, compared to traditional H&E staining. The results showed that PHH3-H&E staining detected more mitotic figures and had better agreement between pathologists and image analysis-assisted software, and PHH3 was identified as an independent predictor of survival.
Article
Cell Biology
Marion Bendayan, Liliana Caceres, Emine Sais, Nelly Swierkowski-Blanchard, Laura Alter, Amelie Bonnet-Garnier, Florence Boitrelle
Summary: This study found that spermatozoa carrying large vacuoles possess specific epigenetic marks that may influence embryo development. High-magnification microscopy can be used as a simple tool to estimate the sperm epigenetic profile, and selecting normal spermatozoa without vacuoles appears to be favorable for embryo development and safe offspring.
Article
Cell Biology
Fang Zhang, Mert Icyuz, Andrzej Bartke, Liou Y. Sun
Summary: The study showed that GH intervention during early life significantly altered histone H3 markers in Ames dwarf mice, resulting in tissue-specific changes in H3 lysine methylation and acetylation. These findings indicate an important epigenetic mechanism by which hormonal signals during early life can influence aging and longevity in mammals.
Article
Plant Sciences
Hasan Mehraj, Satoshi Takahashi, Naomi Miyaji, Ayasha Akter, Yutaka Suzuki, Motoaki Seki, Elizabeth S. Dennis, Ryo Fujimoto
Summary: Covalent modifications of histone proteins, such as H3K4me3 and H3K36me3, are found to regulate gene expression in Brassica rapa L. Two lines of this species showed enrichment of these histone marks at transcription start sites, with gene expression levels being influenced by them. Genes with both H3K4me3 and H3K36me3 typically showed constitutive high expression levels. Additionally, bivalent active and repressive histone modifications were observed in some genes.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Marine & Freshwater Biology
Fan Wei, Bo Pan, Jinghan Diao, Yuanyuan Wang, Yalan Sheng, Shan Gao
Summary: Clipping of the histone H3 N-terminal tail is a significant process in eukaryotes, with the Tetrahymena thermophila organism being the first to exhibit this activity. H3 clipping leads to changes in chromatin dynamics and gene expression, and is believed to be involved in epigenetic regulation and associated with histone modifications and cell cycle regulation.
MARINE LIFE SCIENCE & TECHNOLOGY
(2022)
Review
Oncology
Ting Wen, Qiao Yi Chen
Summary: This article discusses the translation regulatory mechanisms of histone H3.1 and H3.3 and their roles in tumorigenesis.
FRONTIERS IN ONCOLOGY
(2022)
Article
Anatomy & Morphology
Ilhan Elmaci, Ayhan Bilir, Aysel Ozpinar, Meric A. Altinoz
Review
Biochemistry & Molecular Biology
Meric A. Altinoz, Gulacti Topcu, Ahmet Hacimuftuoglu, Alp Ozpinar, Aysel Ozpinar, Emily Hacker, Ilhan Elmaci
NEUROCHEMICAL RESEARCH
(2019)
Review
Immunology
Meric A. Altinoz, Aysel Ozpinar, Alp Ozpinar, Emily Hacker, Ilhan Elmaci
INTERNATIONAL IMMUNOPHARMACOLOGY
(2020)
Article
Toxicology
G. Egimezer, U. Ustundag, P. S. Ates, I Unal, F. D. Ustundag, A. A. Alturfan, E. Emekli-Alturfan, M. A. Altinoz, I Elmaci
HUMAN & EXPERIMENTAL TOXICOLOGY
(2020)
Article
Neurosciences
Ismail Unal, Esin Caliskan-Ak, Unsal V. Ustundag, Perihan S. Ates, Ahmet A. Alturfan, Meric A. Altinoz, Ilhan Elmaci, Ebru Emekli-Alturfan
INTERNATIONAL JOURNAL OF NEUROSCIENCE
(2020)
Review
Clinical Neurology
Ilhan Elmaci, Meric A. Altinoz, Burcu Eylem Kahraman Ozlu, Ramazan Sari, Ozlem Er, Ayca Ersen Danyeli, Ercan Karaarslan
Summary: Benign metastatic leiomyomas (BML) are slowly growing metastatic tumors commonly associated with uterine leiomyomas in women of reproductive ages. Despite their benign pathological features, these tumors can cause significant morbidity and even mortality. Treatment options include hysterectomy and GnRH modifier therapies, with the potential for systemic estrogen antagonists to provide better tumor control.
NEUROSURGICAL REVIEW
(2021)
Review
Genetics & Heredity
Yucel Baspinar, Ilhan Elmaci, Aysel Ozpinar, Meric A. Altinoz
Article
Engineering, Biomedical
Ramazan Sari, Fatma Kubra Erbay Elibol, Eylem Burcu Kahraman Ozlu, Ilhan Elmaci, Teyfik Demir
CLINICAL BIOMECHANICS
(2020)
Review
Biochemistry & Molecular Biology
Meric A. Altinoz, Ilhan Elmaci, Ahmet Hacimuftuoglu, Alp Ozpinar, Emily Hacker, Aysel Ozpinar
Summary: This review study focuses on the potential benefits of the transcription factor PPAR? and its ligand erucic acid (EA) in the management of neuroectodermal tumors and Parkinson's Disease, suggesting that they may play a positive role in these conditions.
MOLECULAR ASPECTS OF MEDICINE
(2021)
Article
Oncology
Meric A. Altinoz, Yasemin Ucal, Muazzez C. Yilmaz, Irem Kiris, Ozan Ozisik, Ugur Sezerman, Aysel Ozpinar, Ilhan Elmaci
Article
Clinical Neurology
Ilhan Elmaci, Meric A. Altinoz, Gokhan Akdemir, Ramazan Sari, Ozdil Baskan, Alp Ozpinar, Emily Hacker, Aydin Sav
Summary: IHSP is a relatively novel entity of hypertrophic pachymeningitis that may present with neurological deficits and spinal compression, treatment involves medication and surgery for management.
CLINICAL NEUROLOGY AND NEUROSURGERY
(2021)
Article
Emergency Medicine
Ramazan Sari, Fatih Han Bolukbasi, Eylem Burcu Kahraman Ozlu, Nejat Isik, Melek Gura Celik, Ilhan Elmaci
ULUSAL TRAVMA VE ACIL CERRAHI DERGISI-TURKISH JOURNAL OF TRAUMA & EMERGENCY SURGERY
(2020)