Article
Biochemistry & Molecular Biology
Zhuoyang Li, Diyi Fu, Xi Wang, Rong Zeng, Xuan Zhang, Jinge Tian, Shuaisong Zhang, Xiaohong Yang, Feng Tian, Jinsheng Lai, Yiting Shi, Shuhua Yang
Summary: This study identifies a negative regulator, bZIP68, that negatively regulates cold tolerance in maize and reveals its differential expression in response to cold stress between maize and its wild ancestor teosinte, which is attributed to natural variation in the bZIP68 promoter.
Article
Plant Sciences
Kora Vyse, Stephanie Schaarschmidt, Alexander Erban, Joachim Kopka, Ellen Zuther
Summary: This study investigates the metabolic and transcriptomic changes in plants during cold priming and triggering. The research findings suggest that genes involved in cell wall remodeling and stress response regulation are induced during triggering, while the expression of late embryogenesis abundant proteins and antifreeze proteins is higher during priming. Moreover, the study indicates that a cold triggering stimulus might be perceived as a milder stress compared to priming in plants.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Biotechnology & Applied Microbiology
Attila Farkas, Bernadett Pap, Otto Zsiros, Roland Patai, Prateek Shetty, Gyozo Garab, Tibor Biro, Vince Ordog, Gergely Maroti
Summary: High salinity poses a challenge for microorganisms in freshwater ecosystems. This study investigated the abilities of freshwater green algae from the Chlorophyta clade to survive and adapt to increased salt concentrations. Different algae species showed varying responses to the elevated salt levels, with Chlorella sp. MACC-360 displaying high salt tolerance and maintaining normal growth and photosynthetic efficiency even at 600 mM NaCl concentration. The tested algae exhibited diverse physiological responses, including changes in growth capacity, morphology, photosynthetic machinery, and reactive oxygen species production. Special alterations were also observed in lipid and exopolysaccharide production patterns. Overall, Chlorella sp. MACC-360 with its outstanding salt tolerance and lipid-producing phenotype holds promise for biotechnological applications.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Agricultural Engineering
Kaiquan Mao, Jianlong Li, Shuhua Wu, Jiajia Qian, Yinyin Liao, Lanting Zeng, Dachuan Gu
Summary: Cold stress can have a serious impact on the yield and quality of spring tea, leading to decreased economic benefits. This study found that treating mature tea plants with exogenous melatonin can protect them from cold stress in tea gardens without significantly affecting the quality of spring tea. Furthermore, the combination of melatonin and cover treatments was effective in promoting the growth of spring tea.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Nelso P. Navarro, Pirjo Huovinen, Jocelyn Jofre, Ivan Gomez
Summary: Early stages of macroalgae are more vulnerable to environmental changes, with diploid carpospores showing higher photosynthetic activity under UV radiation compared to haploid tetraspores. Different responses to radiation between haploid and diploid propagules highlight the ecophysiological versatility of different life-cycle phases in coping with light stress factors for successful recruitment and survival.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Xue Wang, Wen-Cheng Liu, Xue-Wei Zeng, Sa Yan, Yi-Min Qiu, Jin-Bo Wang, Xi Huang, Hong-Mei Yuan
Summary: The study identifies HbSnRK2.6 as a key component in the cold stress response of rubber trees, showing its role in enhancing cold tolerance by regulating the transcriptional activity of HbICE2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Siri Birkeland, Tanja Slotte, Anne Krag Brysting, A. Lovisa S. Gustafsson, Torgeir Rhoden Hvidsten, Christian Brochmann, Michael D. Nowak
Summary: Little is known about the evolution of cold tolerance in polar plant species and how they differ from temperate relatives. This study compared the molecular basis of cold response in three Arctic Brassicaceae species and found that while most differentially expressed genes were species-specific, a significant portion of the cold response was also shared among species. These results suggest that cold response differs between Arctic Brassicaceae species, but builds on a conserved basis found across the family.
Article
Environmental Sciences
Youcai Zhou, Yongjin He, Zhihua Zhou, Xuehua Xiao, Mingzi Wang, Bilian Chen
Summary: This study established a practical approach to efficiently remove ammonium nitrogen from rare earth elements (REEs) wastewater using the indigenous microalga Chlamydomonas sp. YC. Continuous cultivation of Chlamydomonas sp. YC showed superior performance in NH4+-N removal rates and biomass production, making it a promising method for the simultaneous removal of NH4+-N and production of valuable biomass for sustainable biofuels.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Plant Sciences
Huizhen Dong, Qiming Chen, Yuqin Dai, Wenjie Hu, Shaoling Zhang, Xiaosan Huang
Summary: A comprehensive analysis identified the bHLH genes in Chinese white pear, revealing their important roles in response to abiotic stresses, with PbrbHLH195 playing a key role in producing ROS under cold stress, indicating the essential function of PbrbHLH family members in stress tolerance of pear.
Article
Biochemistry & Molecular Biology
Jelena Muncan, Balasooriya Mudiyanselage Siriwijaya Jinendra, Shinichiro Kuroki, Roumiana Tsenkova
Summary: The development of non-destructive methods for early detection of cold stress of plants and the identification of cold-tolerant cultivars is highly needed in crop breeding programs. In this study, soybean leaves' spectra were obtained to understand the role of the water molecular system in the early cold stress response of all cultivars. The results showed major changes in the water molecular structure of the soybean leaves, altered carbohydrate and oxidative metabolism, and differences in the cold stress response of different cultivars.
Article
Multidisciplinary Sciences
Muhammad Fasih Khalid, Sajjad Hussain, Muhammad Akbar Anjum, Raphael Morillon, Shakeel Ahmad, Shaghef Ejaz, Mubshar Hussain, Hawa Z. E. Jaafar, Sara T. Alrashood, Alexe Nicolae Ormenisan
Summary: The study found that tetraploid volkamer lemon rootstock (VM4) exhibited better tolerance to water deficit stress compared to diploid (VM2) by maintaining stronger antioxidant defense mechanisms.
Article
Plant Sciences
Xiaohan Yang, Yunchen Luo, Huiru Bai, Xin Li, Si Tang, Xiaoqin Liao, Lei Zhang, Qinglin Liu
Summary: In this study, a cold-induced MYB transcription factor, DgMYB2, was found to enhance cold tolerance in chrysanthemum by increasing the activity of glutathione peroxidase and reducing reactive oxygen species accumulation.
HORTICULTURE RESEARCH
(2022)
Article
Plant Sciences
Xiaoqin Liao, Xingsu Zhang, Xin Li, Yuchen Tian, Qing Yang, Yongyan Wang, Si Tang, Xuanling Luo, Fan Zhang, Lei Zhang, Beibei Jiang, Qinglin Liu
Summary: This study reports the discovery of a newly identified posttranslational modification called lysine malonylation (Kmal), and its role in the response to abiotic stress in plants. By overexpressing and CRISPR-Cas9-mediated gene editing of DgnsLTP1, a nonspecific lipid transfer protein, it was found to confer cold tolerance in chrysanthemum. Further investigation revealed that DgnsLTP1 interacts with a plasma membrane intrinsic protein (PIP) called DgPIP, and through the regulation of DgGPX expression and activity, it enhances chrysanthemum's low-temperature stress tolerance.
Article
Plant Sciences
Yan Yan, Sun Mintao, Ma Si, Feng Qian, Wang Yijia, Di Qinghua, Zhou Mengdi, He Chaoxing, Li Yansu, Gao Lihong, Yu Xianchang
Summary: This study reveals the mechanism of G protein Ga subunit (CsGPA1) in regulating cold tolerance of plants. The results show that CsGPA1 interacts with CsCDL1 and CsCOR413PM2, and positively regulates the expression of brassinolide-related genes. Additionally, CsGPA1 or CsCOR413PM2 inhibition affects Ca (2+) influx at low temperature and subsequently modulates the expression of CsICE-CsCBF.
HORTICULTURE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Hongjun Xie, Mingdong Zhu, Yaying Yu, Xiaoshan Zeng, Guohua Tang, Yonghong Duan, Jianlong Wang, Yinghong Yu
Summary: This study investigated the regulatory mechanism in two rice varieties under low temperature stress using comparative transcriptome analysis. The results showed that the TGMS variety (33S) had a unique cold-resistance system with differentially expressed genes involved in growth and development pathways. This study provides valuable information for understanding low temperature response mechanisms and improving cold-tolerant rice seedlings.
Article
Biotechnology & Applied Microbiology
Seungjib Jeon, Nam Kyu Kang, William I. Suh, Hyun Gi Koh, Bongsoo Lee, Yong Keun Chang
JOURNAL OF APPLIED PHYCOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Nam Kyu Kang, Eun Kyung Kim, Min-Gyu Sung, Young Uk Kim, Byeong-ryool Jeong, Yong Keun Chang
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Article
Biotechnology & Applied Microbiology
Hyun Gi Koh, Nam Kyu Kang, Seungjib Jeon, Sung-Eun Shin, Byeong-ryool Jeong, Yong Keun Chang
BIOTECHNOLOGY FOR BIOFUELS
(2019)
Article
Biotechnology & Applied Microbiology
Ae Jin Ryu, Nam Kyu Kang, Seungjib Jeon, Dong Hoon Hur, Eun Mi Lee, Do Yup Lee, Byeong-ryool Jeong, Yong Keun Chang, Ki Jun Jeong
BIOTECHNOLOGY FOR BIOFUELS
(2020)
Article
Microbiology
Seok Won Jeong, Kwon HwangBo, Jong Min Lim, Seung Won Nam, Bong Soo Lee, Byeong-ryool Jeong, Yong Keun Chang, Won-Joong Jeong, Youn-Il Park
Article
Plant Sciences
Yanhai Gong, Nam K. Kang, Young U. Kim, Zengbin Wang, Li Wei, Yi Xin, Chen Shen, Qintao Wang, Wuxin You, Jong-Min Lim, Suk-Won Jeong, Youn-Il Park, Hee-Mock Oh, Kehou Pan, Eric Poliner, Guanpin Yang, Yonghua Li-Beisson, Yantao Li, Qiang Hu, Ansgar Poetsch, Eva M. Farre, Yong K. Chang, Won-Joong Jeong, Byeong-ryool Jeong, Jian Xu
Review
Biochemical Research Methods
Jae Won Lee, Sangdo Yook, Hyungi Koh, Christopher Rao, Yong-Su Jin
Summary: Efforts have been concentrated on reprogramming regulatory networks to enhance xylose utilization by engineered yeast and activating and enhancing xylose metabolism in oleaginous yeasts. The potential benefits of using xylose for the production of value-added products have also been demonstrated.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Qintao Wang, Yanhai Gong, Yuehui He, Yi Xin, Nana Lv, Xuefeng Du, Yun Li, Byeong-Ryool Jeong, Jian Xu
Summary: We developed a method for serial and precise deletion of large genome fragments in the industrial microalga Nannochloropsis oceanica, which does not affect essential traits but may enhance growth rate and biomass productivity. This approach has the potential to accelerate the construction of minimal genomes for photosynthetic production.
Article
Biotechnology & Applied Microbiology
Seungjib Jeon, Hyun Gi Koh, Jun Muk Cho, Nam Kyu Kang, Yong Keun Chang
Summary: Overexpressing the NADP-dependent malic enzyme NsME1 in Nannochloropsis salina can increase biomass and fatty acid methyl ester (FAME) contents, leading to a higher FAME yield. This overexpression enhances carbon concentration and reducing power in cells, thus improving lipid and FAME yields of Nannochloropsis salina.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Qintao Wang, Yanbin Feng, Yandu Lu, Yi Xin, Chen Shen, Li Wei, Yuxue Liu, Nana Lv, Xuefeng Du, Wenqiang Zhu, Byeong-ryool Jeong, Song Xue, Jian Xu
Summary: This study identified methods to customize the chain length of fatty acids in Nannochloropsis, enabling the production of designer lipids with tailored properties. A mechanistic model was proposed to manipulate carbon distribution among fatty acids, laying the foundation for scalable production of designer lipids via industrial oleaginous microalgae.
METABOLIC ENGINEERING
(2021)
Review
Agricultural Engineering
Nam Kyu Kang, Kwangryul Baek, Hyun Gi Koh, Christine Anne Atkinson, Donald R. Ort, Yong-Su Jin
Summary: Microalgae are promising sustainable resources that can convert CO2 into biofuels and chemicals directly, but their industrial production and economic feasibility are still limited. Metabolic engineering approaches have been used to enhance the productivity of microalgal bioproducts, with impressive advances in the last decade. Challenges remain in achieving effective production of chemicals and fuels via microalgal metabolic engineering.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Ae Jin Ryu, Byeong-ryool Jeong, Nam Kyu Kang, Seungjib Jeon, Min Gi Sohn, Hyo Jin Yun, Jong Min Lim, Seok Won Jeong, Youn-Il Park, Won Joong Jeong, Sunghoon Park, Yong Keun Chang, Ki Jun Jeong
Summary: Establishing a safe harboring transgene expression system in Nanno-chloropsis microalgae enabled significantly higher expression of the FAD12 gene and increased production of linoleic acid (LA) and eicosapentaenoic acid (EPA). This approach provides excellent proof of concept for successful genetic/metabolic engineering in microalgae and other organisms.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Minsik Kim, Seungjib Jeon, Nam Kyu Kang, Hyung-Gwan Lee, Won-Sub Shin, Hyun Gi Koh, Jin-Ho Yun, Chi-Yong Ahn, Hee-Mock Oh, Yong Keun Chang
Summary: The study investigated the fructose utilization of Ettlia sp. by analyzing the transcription levels of genes related to sugar transport and glycolysis pathway. Significant upregulation of fructokinase and glucokinase genes, as well as a sugar transporter gene (EttSTF11), was observed under fructose supplementation conditions. Phylogenetic analysis showed that EttSTF11 plays a critical role in fructose consumption and metabolism in Ettlia sp.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Plant Sciences
Eric Poliner, Andrea W. U. Busch, Linsey Newton, Young Uk Kim, Rachel Clark, Sofia C. Gonzalez-Martinez, Byeong-Ryool Jeong, Beronda L. Montgomery, Eva M. Farre
Summary: The study found that blue light induces the expression of fatty acid desaturase genes in stramenopile microalgae, promoting the synthesis of EPA. The promoters of blue light-induced genes are enriched with E-box-like motifs. Aureochromes, a specific protein in stramenopiles, act as blue light-regulated transcription factors by sensing blue light.
Article
Agricultural Engineering
Byeong-ryool Jeong, Junhwan Jang, EonSeon Jin
Summary: CRISPR-Cas has revolutionized genetic modification with its simplicity and accuracy, and can be applied at the genomic level. Microalgae are excellent feedstocks for biofuels and nutraceuticals due to their high levels of fatty acids, carotenoids, and other metabolites. However, microalgal engineering is not as advanced as other model organisms. This review proposes further genome engineering schemes, including the construction of sgRNA libraries, pan-genomic and epigenomic resources, and mini-genomes, to develop synthetic biology for carbon-based engineering in microalgae.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Sung-Yeoul Kim, Vikash Chandra Roy, Jin-Seok Park, Byung-Soo Chun
Summary: This study aims to extract functional substances from Undaria pinnatiida sporophyll using supercritical carbon dioxide (SC-CO2) and subcritical water extraction (SWE). The results showed that the consecutive green extraction method can yield extracts rich in fucoxanthin and beta-carotene, and the sporophyll of Undaria pinnatiida contains health-beneficial fatty acids. Additionally, SWE can extract substances with antioxidant, antibacterial, and antihypertensive activities.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2024)