Article
Microbiology
Michael Marner, Laura Kolberg, Julia Horst, Nils Boehringer, Johannes Huebner, I. Dewa M. Kresna, Yang Liu, Ute Mettal, Lei Wang, Melanie Meyer-Buehn, Sanja Mihajlovic, Matthias Kappler, Till F. Schaeberle, Ulrich von Both
Summary: Antimicrobial resistance poses an increasing threat to healthcare. To address the need for innovative treatment of respiratory infections, antibiotics with new modes of action need to be developed. This study generated the antibiogram of clinical isolates from cystic fibrosis patients and identified bicyclic heptapeptides that inhibit the outer membrane protein BamA, showing strong activity against multidrug-resistant strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Haley J. Appaneal, Aisling R. Caffrey, Vrishali Lopes, Emily C. Piehl, Laura A. Puzniak, Kerry L. LaPlante
Summary: We studied antibiotic co-resistance patterns in a national group of hospitalized patients with multidrug-resistant Pseudomonas aeruginosa infections. Co-resistance to commonly used antibiotic classes was common, making it difficult to select appropriate antibiotics for treatment. Poor outcomes were observed for all patients, regardless of different patterns of co-resistance. New antibiotics are needed to help treat hospitalized patients with multidrug-resistant P. aeruginosa infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Fernanda Jimenez Otero, Dianne K. Newman, Leonard M. Tender
Summary: Biofilms provide a protective environment but also present challenges to the cells living within them. Pseudomonas aeruginosa overcomes oxygen limitation by secreting redox active phenazines, which act as electron shuttles. Blocking the re-oxidation of one of these electron shuttles, pyocyanin, decreases cell survival within biofilms and enhances the effect of gentamicin in killing cells. The study highlights the importance of redox cycling of electron shuttles in P. aeruginosa biofilms.
Article
Infectious Diseases
Tomasz Bogiel, Malgorzata Prazynska, Joanna Kwiecinska-Pirog, Agnieszka Mikucka, Eugenia Gospodarek-Komkowska
Summary: The study compared the frequency of selected enzymatic virulence factor genes among non-duplicated carbapenem-resistant P. aeruginosa isolates, finding a higher prevalence of alkaline protease gene and a 37.4% detection rate for neuraminidase-1 gene. Significant differences in the prevalence of lasB and nan1 were observed between MBL-positive and MBL-negative strains.
Article
Biotechnology & Applied Microbiology
James Redfern, Janine Wallace, Alex van Belkum, Magali Jaillard, Elliot Whittard, Roobinidevi Ragupathy, Joanna Verran, Peter Kelly, Mark Charles Enright
Summary: Pseudomonas aeruginosa is a common environmental microorganism that can form biofilms on indwelling device surfaces, promoting adhesion and survival. Research identified accessory genes and core-genome SNPs associated with enhanced biofilm production at 22 degrees C, including a 165 kb genomic island with heavy metal resistance genes.
Article
Microbiology
Yiwei Zhu, Jie Chen, Han Shen, Zhongju Chen, Qi-Wen Yang, Jin Zhu, Xi Li, Qing Yang, Feng Zhao, Jingshu Ji, Heng Cai, Yue Li, Linghong Zhang, Sebastian Leptihn, Xiaoting Hua, Yunsong Yu
Summary: Klebsiella pneumoniae carbapenemase (KPC)-producing Pseudomonas aeruginosa (KPC-PA) strains were found in 40.4% of carbapenem-resistant P. aeruginosa (CRPA) isolates, with half of them resistant to ceftazidime-avibactam (CAZ-AVI). Eight plasmid types and two mobile genetic elements mediating bla(KPC-2) transmission were identified through sequencing. Curing bla(KPC-2) plasmids in 28 strains restored CAZ-AVI susceptibility, indicating its role in resistance.
Article
Respiratory System
Said M. Daboor, John R. Rohde, Zhenyu Cheng
Summary: This study demonstrated the alginate lyase AlyP1400 purified from a marine Pseudoalteromonas bacterium had anti-biofilm activity against Pseudomonas aeruginosa biofilms, which, when combined with antibiotics, reduced biofilm biomass and enhanced the bactericidal activity of antibiotics. The findings suggest the potential value of combinational use of alginate lyase and antibiotics for treating CF lung infections or other P. aeruginosa biofilm-related infections.
JOURNAL OF CYSTIC FIBROSIS
(2021)
Article
Infectious Diseases
Katie A. Parsels, Keri A. Mastro, Jeffrey M. Steele, Stephen J. Thomas, Wesley D. Kufel
Summary: Cefiderocol is a novel siderophore cephalosporin that has shown activity against clinically relevant MDR Gram-negative bacteria. FDA-approved indications include complicated urinary tract infections and hospital-acquired bacterial pneumonia. Clinical trials have demonstrated non-inferiority to standard treatments, but higher all-cause mortality was observed in certain cases.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Chemistry, Physical
Jong Min An, Sangrim Kang, Chang Woo Koh, Sungnam Park, Myung Sook Oh, Dokyoung Kim
Summary: A new nano-antibiotic formulation based on sceptrin-Au nano-aggregates (SANA) has been developed to combat drug-resistant bacterial infections. SANA showed superior antibiotic/antibiofilm activity with low toxicity to red blood cells and endothelial cells. The feasibility of SANA as a new nano-antibiotic was demonstrated in CRPA-contaminated medical supplies.
NANOSCALE HORIZONS
(2022)
Article
Ophthalmology
Maria Cabrera-Aguas, Pauline Khoo, Stephanie L. Watson
Summary: The purpose of this study was to describe the demographics, predisposing factors, clinical features, treatment, and outcomes of microbial keratitis cases resistant to antimicrobials. The results showed that patients with resistant CoNS were younger and presented with smaller ulcers and moderate vision loss. Visual and clinical outcomes were significantly better for resistant CoNS than for those with resistant S. aureus or Corynebacterium spp.
Article
Microbiology
Kimberly K. Kajihara, Homer Pantua, Hilda Hernandez-Barry, Meredith Hazen, Kiran Deshmukh, Nancy Chiang, Rachana Ohri, Erick R. Castellanos, Lynn Martin, Marissa L. Matsumoto, Jian Payandeh, Kelly M. Storek, Kellen Schneider, Peter A. Smith, Michael F. T. Koehler, Siao Ping Tsai, Richard Vandlen, Kelly M. Loyet, Gerald Nakamura, Thomas Pillow, Dhaya Seshasayee, Sharookh B. Kapadia, Wouter L. W. Hazenbos
Summary: This study demonstrates that an anti-P. aeruginosa AAC can locally concentrate antibiotic and kill P. aeruginosa inside phagocytes, providing additional therapeutic options for antibiotics that are moderately active or have an unfavorable pharmacokinetics or toxicity profile. This approach may provide new therapeutic options for antibiotics that are dose limited.
Article
Multidisciplinary Sciences
Biljana Mojsoska, Melanie Ghoul, Gabriel G. Perron, Havard Jenssen, Fatima AlZahra'a Alatraktchi
Summary: This study explores the impact of sub-inhibitory antibiotic concentrations on the production of virulence factors in environmental Pseudomonas aeruginosa strains, showing that sub-inhibitory antibiotic concentrations can alter bacterial toxin production, with strain-specific effects. Additionally, biologically relevant measured toxin concentrations were found to decrease the viability of cultured epithelial cells. This suggests that antibiotic-induced virulence should be considered in routine pathogen testing.
Article
Multidisciplinary Sciences
Remi Le Guern, Teddy Grandjean, Sarah Stabler, Marvin Bauduin, Philippe Gosset, Eric Kipnis, Rodrigue Dessein
Summary: Gut microbiome dysbiosis caused by carbapenemase-producing Enterobacterales (CPE) colonisation is associated with worsened outcomes of Pseudomonas aeruginosa lung infection in mice. The dysbiosis is characterised by decreased alveolar macrophages and conventional dendritic cells, as well as a decrease in the Muribaculaceae family and lower levels of short-chain fatty acids. Faecal microbiota transplantation restores immune responses and lung infection outcomes, indicating the involvement of CPE-induced gut dysbiosis in altering the gut-lung immune axis.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Jonathan D. Cogen, Anna Faino, Frankline Onchiri, Lucas R. Hoffman, Matthew P. Kronman, David P. Nichols, Margaret Rosenfeld, Ronald L. Gibson
Summary: A retrospective cohort study found that there were no significant differences in respiratory and clinical outcomes between young patients with cystic fibrosis treated with one versus two intravenous antipseudomonal antibiotics for pulmonary exacerbations.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Immunology
Jonathan D. Cogen, Frankline M. Onchiri, Nicole Mayer Hamblett, Ronald L. Gibson, Wayne J. Morgan, Margaret Rosenfeld
Summary: The study showed that prolonged use of oral antibiotics in children with cystic fibrosis increased the risk of acquiring Achromobacter xylosoxidans, while treatment with intravenous antibiotics was associated with an increased risk of acquiring multidrug-resistant Pseudomonas aeruginosa and MRSA.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Agriculture, Dairy & Animal Science
H. -K. Wei, H. -X. Xue, Z. X. Zhou, J. Peng
Article
Veterinary Sciences
Yi Zou, Xiao Ming Hu, Ting Zhang, Hong Kui Wei, Yuan Fei Zhou, Zhong Xin Zhou, Jian Peng
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2017)
Article
Polymer Science
Xuliang Luo, Ziran Jiang, Niya Zhang, Zixin Yang, Zhongxin Zhou
Article
Biochemistry & Molecular Biology
Zhongxin Zhou, Anna Zheng, Jianjiang Zhong
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2011)
Article
Biotechnology & Applied Microbiology
Zhen-hua Wei, Ying-yi Duan, Yong-qing Qian, Xiao-feng Guo, Yan-jun Li, Shi-he Jin, Zhong-Xin Zhou, Sheng-yan Shan, Chun-ru Wang, Xue-jiao Chen, Yuguo Zheng, Jian-Jiang Zhong
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2014)
Article
Biochemistry & Molecular Biology
Zhongxin Zhou, Zheng Yin, Xiaoqing Hu
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Zhongxin Zhou, Dafu Wei, Yanhua Lu
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2015)
Article
Biotechnology & Applied Microbiology
Z. X. Zhou, D. F. Wei, Y. Guan, A. N. Zheng, J. J. Zhong
JOURNAL OF APPLIED MICROBIOLOGY
(2010)
Article
Materials Science, Biomaterials
Zhongxin Zhou, Dafu Wei, Yong Guan, Anna Zheng, Jian-Jiang Zhong
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2011)
Review
Food Science & Technology
Zhongxin Zhou, Jie Huang, Haihong Hao, Hongkui Wei, Yuanfei Zhou, Jian Peng
JOURNAL OF FUNCTIONAL FOODS
(2019)
Article
Agriculture, Dairy & Animal Science
J. Huang, X. F. Tong, Z. W. Yu, Y. P. Hu, L. Zhang, Y. Liu, Z. X. Zhou
Article
Biochemistry & Molecular Biology
Jie Huang, Yanping Hu, Xiaofeng Tong, Lei Zhang, Zhengwang Yu, Zhongxin Zhou
Meeting Abstract
Agriculture, Dairy & Animal Science
Jie Huang, Lei Zhang, Zhongxin Zhou
JOURNAL OF ANIMAL SCIENCE
(2019)
Meeting Abstract
Agriculture, Dairy & Animal Science
Jie Huang, Lei Zhang, Zhongxin Zhou
JOURNAL OF ANIMAL SCIENCE
(2019)