Article
Immunology
Sabrina Mazouz, Maude Boisvert, Mohamed S. Abdel-Hakeem, Omar Khedr, Julie Bruneau, Naglaa H. Shoukry
Summary: Researchers analyzed the CD8(+) TCR repertoire specific to HCV during acute infection and found 13 new public human CD8(+) TCR clonotypes unique to HCV that expanded during acute infection and reinfection. The low frequency of cross-reactive TCRs suggests that they are not major determinants of infectious outcome.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Dorothea Bankwitz, Akash Bahai, Maurice Labuhn, Mandy Doepke, Corinne Ginkel, Tanvi Khera, Daniel Todt, Luisa J. Stroh, Leona Dold, Florian Klein, Frank Klawonn, Thomas Krey, Patrick Behrendt, Markus Cornberg, Alice C. McHardy, Thomas Pietschmann
Summary: Neutralising antibodies play a crucial role in infection-induced and vaccine-induced immunity. This study developed HCV reference viruses for quantification of neutralising antibodies and investigated viral functional diversity using human specimens and HCV strains. Results showed representative virus isolates broadly reconstruct the functional HCV neutralisation space, enabling high resolution profiling of HCV neutralisation.
Article
Microbiology
Ju Jia, Hui Li, Zhisheng Huang, Jiapei Yu, Ying Zheng, Bin Cao
Summary: This study investigates the transitions and regulation mechanisms of CD8(+) T cells after influenza virus infection and reinfection. The findings show that CD8(+) Trm cells accumulate in the lung after infection, with a subset of these cells maintaining high expression of CD49a even 90 days after primary infection. The ratio of CD8(+) Trm cells decreases after reinfection, potentially indicating a transition into effector types. Additionally, various signaling pathways and cell interactions, including the Ccl5-Ccr5 ligand/receptor pair, play important roles in the function and interactions of CD8(+) Trm cells after infection and reinfection.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Akira Nishio, Sharika Hasan, Heiyoung Park, Nana Park, Jordan H. Salas, Eduardo Salinas, Lela Kardava, Paul Juneau, Nicole Frumento, Guido Massaccesi, Susan Moir, Justin R. Bailey, Arash Grakoui, Marc G. Ghany, Barbara Rehermann
Summary: The humoral response to HCV decreases in potency and breadth after cure of chronic hepatitis C, while HCV-specific memory B cells persist. This finding has implications for the development of an effective vaccine against HCV infection.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Jennifer Currenti, Joshua Simmons, Jared Oakes, Silvana Gaudieri, Christian M. Warren, Rama Gangula, Eric Alves, Ramesh Ram, Shay Leary, Jesse D. Armitage, Rita M. Smith, Abha Chopra, Natasha B. Halasa, Mark A. Pilkinton, Spyros A. Kalams
Summary: This study examined the T cell receptor (TCR) diversity and transcriptional profile of vaccine-induced expanded circulating T follicular helper (cTfh) cells in individuals who received sequential seasonal influenza vaccines. The results showed that the percentage of activated cTfh cells significantly increased from day 0 to day 7 post-vaccination in each vaccine season, and the magnitude of cTfh activation increase was positively correlated with the frequency of circulating plasmablast cells. Higher magnitudes of cTfh activation were associated with increased clonality of cTfh TCR repertoire at day 7 post-vaccination.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Behzad Hajarizadeh, Jason Grebely, Marianne Byrne, Pip Marks, Janaki Amin, Hamish McManus, Tony Butler, Evan B. Cunningham, Peter Vickerman, Natasha K. Martin, John G. McHutchison, Diana M. Brainard, Carla Treloar, Georgina M. Chambers, Luke Grant, Colette Mcgrath, Andrew R. Lloyd, Gregory J. Dore
Summary: The Surveillance and Treatment of Prisoners with hepatitis C (SToP-C) study aimed to assess the effect of HCV treatment-as-prevention in the prison setting and showed that direct-acting antiviral (DAA) treatment scale-up was associated with reduced HCV incidence in prison, supporting broad DAA treatment scale-up within incarcerated populations.
LANCET GASTROENTEROLOGY & HEPATOLOGY
(2021)
Article
Immunology
Dor Yoeli, Rashikh A. Choudhury, Hunter B. Moore, Angela Sauaia, Mary Ann Simpson, Elizabeth A. Pomfret, Trevor L. Nydam
Summary: This study found that in the era of direct-acting antivirals, HCV-positive female liver transplant recipients had a reduced risk of graft failure and comparable outcomes to HCV-negative female recipients.
Article
Multidisciplinary Sciences
Mladen Jergovic, Christopher P. Coplen, Jennifer L. Uhrlaub, David G. Besselsen, Shu Cheng, Megan J. Smithey, Janko Nikolich-Zugich
Summary: Infections can lead to the expansion of a subpopulation of long-lived, Ly6C(+) CD8(+) Tn cells with accelerated effector function, depending on the action of IFN-I. This highlights the significant role of infection-driven IFN-I in regulating Tn homeostasis and function, with potential implications for immunotherapy.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Hiroshi Ishii, Kazutaka Terahara, Takushi Nomura, Midori Okazaki, Hiroyuki Yamamoto, Tsugumine Shu, Hiromi Sakawaki, Tomoyuki Miura, David I. Watkins, Tetsuro Matano
Summary: This study demonstrates that inducing virus-specific CD8(+) T cells rather than CD4(+) T cells through specific immunogen design can enhance the protective efficacy of HIV vaccines.
Article
Gastroenterology & Hepatology
Peng Huang, Yan Wang, Ming Yue, Zhijun Ge, Xueshan Xia, Andre J. Jeyarajan, Jacinta A. Holmes, Rongbin Yu, Chuanwu Zhu, Sheng Yang, Wenyu Lin, Raymond T. Chung
Summary: The HCV recurrence risk after achieving SVR with all-oral DAAs therapy is low, and the risk of HCV recurrence in high-risk and HIV/HCV-coinfected populations was driven by an increase in reinfection rather than late relapse.
LIVER INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Abdalla Sheikh, Jennie Jackson, Hanjoo Brian Shim, Clement Yau, Jung Hee Seo, Ninan Abraham
Summary: Interleukin-7 (IL-7) plays an important role in shaping CD8 T cell responses during acute viral infections. IL-7 is required for proper expansion and effector functions of influenza-specific CD8 T cells in the mediastinal lymph node. IL-7 also affects terminal differentiation, degranulation, and cytokine production of specific CD8 T cells. Understanding IL-7's role in respiratory diseases will help expand therapeutic options.
SCIENTIFIC REPORTS
(2022)
Review
Medicine, General & Internal
Marianne Martinello, Sunil S. Solomon, Norah A. Terrault, Gregory Dore
Summary: Hepatitis C virus (HCV) is a serious liver disease that can lead to hepatitis and hepatocellular carcinoma. WHO has called for the elimination of HCV infection by 2030, but there are still a significant number of infections and deaths. The development of direct-acting antiviral therapy shows promise, but efforts are needed to strengthen screening, linkage to care, and treatment.
Article
Gastroenterology & Hepatology
Janine Kemming, Swantje Gundlach, Marcus Panning, Daniela Huzly, Jiabin Huang, Marc Luetgehetmann, Sven Pischke, Julian Schulze Zur Wiesch, Florian Emmerich, Sian Llewellyn-Lacey, David A. Price, Yakup Tanriver, Klaus Warnatz, Tobias Boettler, Robert Thimme, Maike Hofmann, Nicole Fischer, Christoph Neumann-Haefelin
Summary: Chronic HEV infection is associated with exhaustion of HEV-specific CD8+ T cells, indicating that T-cell failure is driven by persistent antigen recognition in severely immunosuppressed hosts. Functional reinvigoration of virus-specific T cells is at least partially possible when the antigen is cleared. Viral escape in a minority of patients also contributes to the failure of HEV-specific CD8+ T cells.
JOURNAL OF HEPATOLOGY
(2022)
Article
Gastroenterology & Hepatology
Tobias Boettler, Benedikt Csernalabics, Henrike Salie, Hendrik Luxenburger, Lara Wischer, Elahe Salimi Alizei, Katharina Zoldan, Laurenz Krimmel, Peter Bronsert, Marius Schwabenland, Marco Prinz, Carolin Mogler, Christoph Neumann-Haefelin, Robert Thimme, Maike Hofmann, Bertram Bengsch
Summary: This case report highlights the occurrence of autoimmune hepatitis episodes following COVID-19 vaccination in a patient with distinctive T cell-dominant immune-mediated hepatitis. Liver tissue analysis revealed an immune infiltrate with activated cytotoxic CD8 T cells predominantly distributed, indicating a unique pathomechanism associated with vaccination-induced antigens.
JOURNAL OF HEPATOLOGY
(2022)
Article
Gastroenterology & Hepatology
William Bremer, Heather Blasczyk, Xin Yin, Eduardo Salinas, Arash Grakoui, Zongdi Feng, Christopher Walker
Summary: The study found that CD8+ T cells can assist in controlling HEV infection in untreated macaques, but the simultaneous appearance of CD8+ T cells with HEV clearance may support the role of this subset in controlling virus replication. However, virus clearance in the absence of CD8+ T cells indicates that neutralizing antibodies and/or CD4+ T cells may act autonomously to inhibit HEV replication.
JOURNAL OF HEPATOLOGY
(2021)
Article
Infectious Diseases
Doris G. Ransy, Etienne Lord, Martine Caty, Normand Lapointe, Marc Boucher, Abdoulaye Banire Diallo, Hugo Soudeyns
INFECTION GENETICS AND EVOLUTION
(2018)
Article
Microbiology
Brad R. Rosenberg, Marion Depla, Catherine A. Freije, Denis Gaucher, Sabrina Mazouz, Maude Boisvert, Nathalie Bedard, Julie Bruneau, Charles M. Rice, Naglaa H. Shoukry
Review
Virology
Mohamed S. Abdel-Hakeem
Article
Multidisciplinary Sciences
Jonathan J. Kotzin, Fany Iseka, Jasmine Wright, Megha G. Basavappa, Megan L. Clark, Mohammed-Alkhatim Ali, Mohamed S. Abdel-Hakeem, Tanner F. Robertson, Walter K. Mowel, Leonel Joannas, Vanessa D. Neal, Sean P. Spencer, Camille M. Syrett, Montserrat C. Anguera, Adam Williams, E. John Wherry, Jorge Henao-Mejia
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Immunology
Jean-Christophe Beltra, Sasikanth Manne, Mohamed S. Abdel-Hakeem, Makoto Kurachi, Josephine R. Giles, Zeyu Chen, Valentina Casella, Shin Foong Ngiow, Omar Khan, Yinghui Jane Huang, Patrick Yan, Kito Nzingha, Wei Xu, Ravi K. Amaravadi, Xiaowei Xu, Giorgos C. Karakousis, Tara C. Mitchell, Lynn M. Schuchter, Alexander C. Huang, E. John Wherry
Article
Immunology
Jonathan B. Johnnidis, Yuki Muroyama, Shin Foong Ngiow, Zeyu Chen, Sasikanth Manne, Zhangying Cai, Shufei Song, Jesse M. Platt, Jason M. Schenkel, Mohamed Abdel-Hakeem, Jean-Christophe Beltra, Allison R. Greenplate, Mohammed-Alkhatim A. Ali, Kito Nzingha, Josephine R. Giles, Christelle Harly, John Attanasio, Kristen E. Pauken, Bertram Bengsch, Michael A. Paley, Vesselin T. Tomov, Makoto Kurachi, Dario A. A. Vignali, Arlene H. Sharpe, Steven L. Reiner, Avinash Bhandoola, F. Bradley Johnson, E. John Wherry
Summary: The study identifies a distinct subset of memory T cells during the expansion phase of acute viral infection, which possess unique characteristics and play an important role in CD8(+) T cell memory. This subset, although quantitatively minor in the TCF-1(+) pool, exhibits self-renewal and long-term recall capacity.
SCIENCE IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zeyu Chen, Eri Arai, Omar Khan, Zhen Zhang, Shin Foong Ngiow, Yuan He, Hua Huang, Sasikanth Manne, Zhendong Cao, Amy E. Baxter, Zhangying Cai, Elizabeth Freilich, Mohammed A. Ali, Josephine R. Giles, Jennifer E. Wu, Allison R. Greenplate, Mohamed A. Hakeem, Qingzhou Chen, Makoto Kurachi, Kito Nzingha, Viktoriya Ekshyyan, Divij Mathew, Zhuoyu Wen, Nancy A. Speck, Alexis Battle, Shelley L. Berger, E. John Wherry, Junwei Shi
Summary: Improving the effector activity of antigen-specific T cells is a major goal in cancer immunotherapy. Although several effector T cell-driving transcription factors have been identified, the transcriptional coordination of T-EFF biology remains poorly understood. This study shows that genetic deletion of Fli1 enhances T-EFF responses by restraining T-EFF lineage differentiation.
Article
Virology
Amr Ali Hemeda, Amal Ahmad Mohamed, Ramy Karam Aziz, Mohamed S. Abdel-Hakeem, Marwa Ali-Tammam
Summary: The study found a significant association between specific SNPs in IL-10, SOD2, and ApoE genes and the severity of fibrosis induced by HCV, as well as statistically significant associations between specific haplotype combinations and fibrosis severity. These findings can significantly impact precision prophylaxis and treatment of patients with HCV-induced fibrosis.
Article
Immunology
Sabrina Mazouz, Maude Boisvert, Mohamed S. Abdel-Hakeem, Omar Khedr, Julie Bruneau, Naglaa H. Shoukry
Summary: Researchers analyzed the CD8(+) TCR repertoire specific to HCV during acute infection and found 13 new public human CD8(+) TCR clonotypes unique to HCV that expanded during acute infection and reinfection. The low frequency of cross-reactive TCRs suggests that they are not major determinants of infectious outcome.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Immunology
Mohamed S. Abdel-Hakeem, Sasikanth Manne, Jean-Christophe Beltra, Erietta Stelekati, Zeyu Chen, Kito Nzingha, Mohammed-Alkhatim Ali, John L. Johnson, Josephine R. Giles, Divij Mathew, Allison R. Greenplate, Golnaz Vahedi, E. John Wherry
Summary: Exhausted T cells only partially recover memory T cell characteristics upon chronic antigen elimination, with a failure to restore core memory epigenetic circuits, leading to compromised immune responses upon rechallenge.
Article
Immunology
Josephine R. Giles, Shin Foong Ngiow, Sasikanth Manne, Amy E. Baxter, Omar Khan, Ping Wang, Ryan Staupe, Mohamed S. Abdel-Hakeem, Hua Huang, Divij Mathew, Mark M. Painter, Jennifer E. Wu, Yinghui Jane Huang, Rishi R. Goel, Patrick K. Yan, Giorgos C. Karakousis, Xiaowei Xu, Tara C. Mitchell, Alexander C. Huang, E. John Wherry
Summary: This study used longitudinal single-cell RNA sequencing and single-cell assay for transposase-accessible chromatin sequencing analyses to uncover new subsets of CD8(+) T cells and explore the epigenetic differences in T-ex cell subsets in different infection states.
Article
Biochemistry & Molecular Biology
Hebatalla Said Ali, Mariam Sameh Boshra, Sara H. A. Agwa, Mohamed S. Abdel Hakeem, Mahmoud Shawky El Meteini, Marwa Matboli
Summary: This study identified a group of differentially expressed mRNAs related to the cGAS/STING, NOD-like receptor pathways (NLR), and T2DM using a microarray dataset. Comparative analysis using real-time PCR revealed significantly higher expressions of ZBP1, DDX58, NFKB1, and CHUK in the prediabetes group compared to healthy controls or T2DM patients. The expressions of ZBP1 and NFKB1 mRNA could discriminate between good and poor glycemic control groups.
Article
Virology
Isabelle Bernard, Doris G. G. Ransy, Jason Brophy, Fatima Kakkar, Ari Bitnun, Lindy Samson, Stanley Read, Hugo Soudeyns, Michael T. T. Hawkes
Summary: This study found that CHI3L1 levels in children perinatally infected with HIV are associated with inflammation, viral load, and microbial translocation, suggesting its potential as a marker for systemic inflammation in CLWH.
Article
Infectious Diseases
Isabelle Boucoiran, Helene C. F. Cote, Caroline Jodoin, Chelsea Elwood, Fatima Kakkar, Silvie Valois, Deborah M. Money, Hugo Soudeyns
Summary: This study aimed to determine the frequency and factors associated with CD4 count dropping below 200 cells/mm(3) during pregnancy in women living with HIV. Data from two Canadian cohorts of pregnant women living with HIV were analyzed, and it was found that a drop in CD4 count to <200 cells/mm(3) was uncommon, with factors such as coinfection with hepatitis B or C viruses, lower first visit CD4 counts, and baseline haemoglobin levels <11 g/dL being associated with this drop. The study suggests that monitoring CD4 count once during pregnancy would be safe for women whose CD4 count is >450 cells/mm(3) at the first pregnancy visit.
Article
Pharmacology & Pharmacy
Amal Ahmed Mohamed, Amr Ali Hemeda, Ramy Karam Aziz, Mohamed Salaheldin Abdel-Hakeem, Marwa Ali-Tammam
FUTURE JOURNAL OF PHARMACEUTICAL SCIENCES
(2020)
