Article
Multidisciplinary Sciences
Xiaoli Lin, Chanyi Lu, Makoto Ohmoto, Katarzyna Choma, Robert F. Margolskee, Ichiro Matsumoto, Peihua Jiang
Summary: The neuron-supplied niche factor, R-spondin-2, plays a crucial role in taste bud cell regeneration and maintenance by promoting the generation of differentiated taste cells. It can substitute for neuronal input to maintain taste bud cells and tissues. The absence of R-spondin in culture medium inhibits the generation of taste bud cells ex vivo.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Food Science & Technology
Jiali Lu, Yuezhong Mao, Jiaming Qi, Jianzhong Han, Yumei Qin
Summary: Chronic administration of caffeine in mice led to increased behavioral responses to sweet stimuli, decreased anatomical characteristics of taste buds, and altered expression levels of taste receptor proteins. These changes suggest that chronic caffeine exposure can affect taste sensitivity and behavior.
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
(2021)
Article
Multidisciplinary Sciences
Wenxin Yu, Mohamed Ishan, Zhonghou Wang, Hong-Xiang Liu
Summary: Cell dissociation is essential for studies at both single-cell and cell population levels, with optimal conditions dependent on tissue characteristics. Isolating cells from different regions of the mouse tongue was successful, with viable cells obtained from both epithelial and mesenchymal/connective tissue.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Cell Biology
Ting Zhang, Pin-Xian Xu
Summary: This study reveals that Eya1 and Eya2 play important roles in the development and maintenance of the mouse taste system. Eya1-expressing progenitors differentiate into tongue musculature, while Eya2 is specifically expressed in specific taste papillae. Eya1 and Eya2 may cooperate to promote lineage commitment of taste cell subtypes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Endocrinology & Metabolism
Lisa Y. Lawson, Michael D. Brodt, Nicole Migotsky, Christopher J. Chermside-Scabbo, Ramya Palaniappan, Matthew J. Silva
Summary: The secretion of Wnt ligands is essential for adult bone homeostasis, and inhibiting their secretion results in decreased bone formation and increased resorption. Additionally, osteoblast-derived Wnts play a crucial role in mediating the bone anabolic response to tibial loading.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Makoto Ohmoto, Shugo Nakamura, Hong Wang, Peihua Jiang, Junji Hirota, Ichiro Matsumoto
Summary: This study reveals the dynamics of single stem cells in the turnover of taste bud cells. The Sox2(+) stem cell population is maintained by balancing the increase of certain stem cells with the reduction of others. Taste buds are composed of cells derived from multiple Sox2(+) stem cells.
Article
Biochemistry & Molecular Biology
Jun Yu, Cong Shen, Meng Lin, Xia Chen, Xiuliang Dai, Zhiran Li, Yunhao Wu, Yangbo Fu, Jinxing Lv, Xiaoyan Huang, Bo Zheng, Fei Sun
Summary: In this study, we identified BMI1 as a key regulator of spermatogonial stem cell (SSC) maintenance. BMI1 represses target genes by increasing H2AK119ub and reducing H3K4me3 in SSCs. Additionally, BMI1 inhibition activates Wnt signaling and promotes nuclear translocation of beta-catenin in SSCs. Suppression of Wnt/beta-catenin signaling restores SSC maintenance in BMI1-deficient SSCs. Our findings not only reveal a novel mechanism for BMI1 in SSC maintenance, but also provide a potential strategy for treating male infertility.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Kale S. Bongers, Roderick A. McDonald, Katherine M. Winner, Nicole R. Falkowski, Christopher A. Brown, Jennifer M. Baker, Kevin J. Hinkle, Daniel J. Fergle, Robert P. Dickson
Summary: The microbiome plays a significant role in organismal metabolism, with microbiome modulation primarily mediating metabolic changes in mice treated with antibiotics. While antibiotics have off-target effects on behavior and diet, the weight loss seen in tissue-specific areas following antibiotic administration is primarily due to microbiome effects rather than food and water aversion.
Article
Cell Biology
Yoshifumi Mori, Seiji Takashima, Mito Kanatsu-Shinohara, Zheng Yi, Takashi Shinohara
Summary: The study found that Cdc42 is essential for the development of the germline niche, as its deficiency results in a significant reduction in the number of spermatogonial stem cells in the testes, affecting GDNF expression and Sertoli cell development.
Article
Biology
Sayumi Fujimori, Izumi Ohigashi, Hayato Abe, Yosuke Matsushita, Toyomasa Katagiri, Makoto M. Taketo, Yousuke Takahama, Shinji Takada
Summary: The thymic epithelium plays a crucial role in supporting the development of T cells. This study found that enhanced expression of beta-catenin in thymic epithelial cells leads to thymic dysplasia and T cell deficiency in the embryonic period, while loss of beta-catenin function only slightly reduces cortical thymic epithelial cells and thymocytes in postnatal development.
Article
Medicine, Research & Experimental
Youki Takezawa, Maki Iwai, Yukiko Fujiki, Ryo Yokomizo, Harue Kishigami, Mami Miyado, Natsuko Kawano, Mitsutoshi Yamada, Miyuki Shindo, Miki Suzuki, Ban Sato, Daiki Katano, Shintaro Kamijo, Toshio Hamatani, Mamoru Tanaka, Akihiro Umezawa, Woojin Kang, Kenji Miyado
Summary: Repeated implantation failure is a common cause of infertility in healthy women. The role of embryonic CTNNB1 in implantation is not well understood. Using mouse models, researchers found that Ctnnb1(Delta/Delta) embryos developed to the blastocyst stage but were subsequently resorbed, leaving empty decidual capsules. The absence of leukemia inhibitory factor and CDX2 in Ctnnb1(Delta/Delta) blastocysts indicates the importance of embryonic CTNNB1 in the secretion of blastocyst-derived factors and the proper development of trophectoderm cells. Intrauterine injection of uterine fluids and leukemia inhibitory factor proteins rescued the uterine response to Ctnnb1(Delta/Delta) blastocysts, suggesting a potential therapeutic approach for improving implantation. These findings contribute to a better understanding of the pathogenesis of repeated implantation failure.
LABORATORY INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Mei-Jen Hsieh, Ching-Chieh Weng, Yu-Chun Lin, Chia-Chen Wu, Li-Tzong Chen, Kuang-Hung Cheng
Summary: This study revealed the importance of the Wnt/β-catenin signaling pathway in pancreatic cancer, showing that loss of LKB1 affects cystadenoma development and that the beta-catenin inhibitor FH535 can effectively suppress LKB1 loss-driven cystadenoma and slow down pancreatic cancer progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Jiankui Wang, Kai Cui, Guoying Hua, Deping Han, Zu Yang, Tun Li, Xue Yang, Yuanyuan Zhang, Ganxian Cai, Xiaotian Deng, Xuemei Deng
Summary: In this study, transgenic mice with skin-specific overexpression of ovine beta-catenin were generated through pronuclear microinjection. The successful transfer of the ovine beta-catenin gene into mice and its inheritance through multiple generations were confirmed by PCR testing and Southern blot analysis. The specific expression of beta-catenin mRNA in the skin of transgenic mice was demonstrated through qRT-PCR and Western blot analysis. The overexpression of beta-catenin was found to promote hair follicle development in mice by prematurely transitioning from catagen to anagen, thus increasing hair follicle density.
FRONTIERS IN GENETICS
(2023)
Article
Chemistry, Multidisciplinary
Na Hui Kim, Minji Kwon, Jiwoo Jung, Hyo Byeong Chae, Jiwoo Lee, Yeo-Jun Yoon, In Seok Moon, Ho K. Lee, Wan Namkung, Konstantina M. Stankovic, Se A. Lee, Jong Dae Lee, Sin-Aye Park
Summary: In this study, researchers found that celastrol can inhibit the growth of vestibular schwannoma (VS) by promoting the degradation of beta-catenin through Wnt/beta-catenin signaling pathway. Celastrol showed potent inhibitory effect on the viability of VS cells and suppressed tumor growth without toxicity in mice.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Cell & Tissue Engineering
Hsin-Hsiung Chen, Hao-Yu Lu, Chao-Hsin Chang, Shih-Hao Lin, Chu-Wei Huang, Po-Han Wei, Yi-Wen Chen, Yi-Rou Lin, Hsien-Sung Huang, Pei-Yu Wang, Yeou-Ping Tsao, Show-Li Chen
Summary: This study found that BCAS2 can regulate adult neurogenesis in mice by modulating beta-catenin. BCAS2 cKO mice showed decreased proliferation of neural stem cells and immature neurons. Gene therapy was able to reverse these changes and increase beta-catenin expression.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Genetics & Heredity
Dany Gaillard, Mingang Xu, Fei Liu, Sarah E. Millar, Linda A. Barlow
Article
Multidisciplinary Sciences
Mingang Xu, Jeremy Horrell, Melinda Snitow, Jiawei Cui, Heather Gochnauer, Camille M. Syrett, Staci Kallish, John T. Seykora, Fei Liu, Dany Gaillard, Jonathan P. Katz, Klaus H. Kaestner, Brooke Levin, Corinne Mansfield, Jennifer E. Douglas, Beverly J. Cowart, Michael Tordoff, Fang Liu, Xuming Zhu, Linda A. Barlow, Adam I. Rubin, John A. McGrath, Edward E. Morrisey, Emily Y. Chu, Sarah E. Millar
NATURE COMMUNICATIONS
(2017)
Article
Multidisciplinary Sciences
Dany Gaillard, Patricia Passilly-Degrace, Philippe Besnard
ADDICTION REVIEWS 2008
(2008)
Article
Biochemistry & Molecular Biology
Dany Gaillard, Fabienne Laugerette, Nicolas Darcel, Abdelghani El-Yassimi, Patricia Passilly-Degrace, Aziz Hichami, Naim Akhtar Khan, Jean-Pierre Montmayeur, Philippe Besnard
Article
Developmental Biology
Dany Gaillard, Linda A. Barlow
Article
Multidisciplinary Sciences
Celine Martin, Patricia Passilly-Degrace, Dany Gaillard, Jean-Francois Merlin, Michael Chevrot, Philippe Besnard
Editorial Material
Physiology
Dany Gaillard, Sue C. Kinnamon
Article
Biochemistry & Molecular Biology
Dany Gaillard, David Masson, Erwan Garo, Maamar Souidi, Jean-Paul Pais de Barros, Kristina Schoonjans, Jacques Grober, Philippe Besnard, Charles Thomas
Summary: The study investigated the impact of a high cholesterol diet on cholesterol and bile acid metabolism in mice, revealing that increased levels of muricholic acid contribute to efficient elimination of cholesterol and neo-synthesized bile acids, reducing the occurrence of hypercholesterolemia. This suggests potential therapeutic opportunities using hydrophilic bile acid supplementation to mitigate hypercholesterolemia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
Patricia Passilly-Degrace, Dany Gaillard, Philippe Besnard
CAHIERS DE NUTRITION ET DE DIETETIQUE
(2008)
Article
Biochemistry & Molecular Biology
F. Laugerette, D. Gaillard, P. Passilly-Degrace, I. Niot, P. Besnard
Article
Gastroenterology & Hepatology
C. Thomas, J-F Landrier, D. Gaillard, J. Grober, M-C Monnot, A. Athias, P. Besnard