Article
Medicine, Legal
Iskandar Z. Siregar, Muhammad Jauhari Ramdhani, Lina Karlinasari, Ulfa Adzkia, M. Zainul Arifin, Fifi Gus Dwiyanti
Summary: This study compared the DNA isolation results of various dried and fresh wood samples using two extraction methods (CTAB and Qiagen DNeasy Plant Mini Kit). While dried wood samples showed poor visualization, fresh wood samples had higher DNA concentration in CTAB-extracted samples compared to QDPMK-extracted samples.
Article
Plant Sciences
Aanchal Singh, H. S. Ginwal, Jitendra Kumar, Rajendra Kumar Meena
Summary: A new CTAB-activated charcoal-based method has been developed for extracting high-quality genomic DNA from Terminalia bellirica. The method is rapid, reliable, and versatile, allowing extraction from fresh and desiccated plant materials. The extracted DNA is of exceptional purity and suitable for further analysis.
BRAZILIAN JOURNAL OF BOTANY
(2023)
Article
Multidisciplinary Sciences
Maria Ulfa, Cindy Nur Anggreani, Novia Amalia Sholeha
Summary: Titanium dioxide (TiO2) was incorporated into mesoporous silica (SMG) using a hydrothermal approach and a dual template CTAB-Gelatin. Various characterization techniques were used to evaluate a 1 wt% TiO2/SMG material. The addition of gelatin during synthesis increased the pore volume of SMG, and the TiO2 crystal grains developed on the mesoporous silica-gelatin caused expansion of the silica pores. The TiO2/SMG composite exhibited higher photodegradability for methylene blue compared to TiO2/mesoporous silica without gelatin.
Article
Multidisciplinary Sciences
Jenyfer Jimenez, Ana Maria Leiva, Cristian Olaya, Daniela Acosta-Trujillo, Wilmer Jose Cuellar
Summary: The described method utilizes dry leaf samples stored in silica gel, does not require liquid Nitrogen or phenol extraction, and produces high quality nucleic acid extracts suitable for detection of cassava DNA and RNA viruses.
Article
Multidisciplinary Sciences
Diana Victoria Marin, Diana Katherine Castillo, Luis Augusto Becerra Lopez-Lavalle, Jairo Rodriguez Chalarca, Cristo Rafael Perez
Summary: The optimized high-quality DNA isolation protocol developed for Fall Armyworm body segment tissue allows rapid extraction of high-quality total genomic DNA suitable for subsequent molecular applications.
Article
Chemistry, Analytical
D. Zybin, A. Prostyakova, D. Kapustin
Summary: A one-step DNA isolation method from soil extracts was developed using a two-component bioseparating element, allowing for simultaneous purification of DNA from humic substances. This innovative approach improved DNA purity and isolation efficiency, providing a promising solution to the challenges faced in DNA isolation from soil samples.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Beyhan Erdem, Sezer Erdem, Nalan Tekin
Summary: Three magnetic adsorbents were prepared and characterized with mesoporous silica layers successfully. Both silica and mesoporous silica coating strategies were found to play crucial roles in improving the adsorptive properties of nanocomposites.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Biochemical Research Methods
Runtao Zhong, Mengyu Wang, Bingcheng Lin
Summary: A microfluidic DNA extraction protocol based on integrated diaphragm microvalves/pumps and silica-deposited open-channel columns was developed for automated and parallel DNA solid-phase extraction. The method uses microfluidic chips with a sandwiched structure containing three layers, enabling the processing of multiple samples in parallel without changing the microvalve control module. The integrated microvalves/pumps allow for automated, programmable, and simultaneous DNA extractions from different samples, even from complex solutions, with stable and reliable reuse of the silica-deposited open-channel columns.
Article
Biotechnology & Applied Microbiology
Dora Certnerova
Summary: Microalgae are important organisms for global ecosystem functioning and biotechnology. Knowledge of their nuclear DNA content is crucial for research, but estimating it using flow cytometry can be difficult due to their complex cell structures. Different nuclei isolation methods and solutions were compared, with chopping desiccated biomass in LB01 buffer showing the most success. The study also provided the first DNA content estimates for four investigated taxa.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Construction & Building Technology
Oriana Palacio, Wim J. Malfait, Silvain Michel, Michel Barbezat, Zahra Mazrouei-Sebdani
Summary: Vibration reduction and structure-borne sound insulation are important for creating a healthy housing environment. Silica aerogels, with their low resonance frequency and dynamic stiffness, show superior performance compared to recycled rubber for vibration isolation in construction.
CONSTRUCTION AND BUILDING MATERIALS
(2023)
Article
Plant Sciences
Onkar Nath, Stephen J. Fletcher, Alice Hayward, Lindsay M. Shaw, Rimjhim Agarwal, Agnelo Furtado, Robert J. Henry, Neena Mitter
Summary: This study optimized methods for high-quality DNA extraction from multiple avocado cultivars and RNA extraction from various tissue types. The extracted DNA and RNA were used for sequencing and gene expression analysis, and the methods developed may be applicable to other recalcitrant plant species.
Article
Environmental Sciences
Bahareh Kianfar, Jingya Tian, Joachim Rozemeijer, Bas A. van der Zaan, Thom A. Bogaard, Jan Willem Foppen
Summary: The study explored the deposition kinetics of a new DNA-tagged silica tracer under different pore water chemistries, flow rates, and sand grain size distributions. The results showed that the tracer is sensitive to both physical and chemical factors, making it suitable for studying subsurface flow paths and travel times.
JOURNAL OF CONTAMINANT HYDROLOGY
(2022)
Article
Chemistry, Physical
Bahareh Kianfar, S. Majid Hassanizadeh, Ahmed Abdelrady, Thom Bogaard, Jan Willem Foppen
Summary: Interactions between natural organic matter (NOM) and colloids in terrestrial environments have a significant impact on the transport behavior and ecotoxicity of the colloids. This study investigated the transport and release of a DNA-tagged synthetic colloid as a surrogate for natural colloids in sand columns. The results showed that the deposition rate of the colloid was inversely proportional to the NOM concentration and significantly increased under low ionic strength conditions. Moreover, the DLVO theory underestimated the colloid deposition in the presence of CaCl2. Flushing the columns with Milli-Q water induced colloid remobilization, leading to a secondary peak in the breakthrough curves. Our work highlights the complex interplay between water quality and the deposition and release of colloidal matter in saturated porous media.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Chemistry, Multidisciplinary
Hyo-Won Han, Ara Joe, Eue-Soon Jang
Summary: The study confirmed the cytotoxicity of residual CTAB surfactant on gold nanorods, and demonstrated that amine-functionalized fluorescence dyes could effectively substitute CTAB to reduce toxicity. The resulting products showed excellent surface-enhanced Raman scattering performance and could detect a few cancer cells.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Construction & Building Technology
Hamza Gullu, Mehmet Emin Yetim, Elif Bacak Gullu
Summary: This study investigates the rheological abilities of nano-silica added to Portland cement based-grouts for ground improvement. The results indicate that low to moderate dosages of nano-silica (<2.5%NS) at a water/binder ratio of 1 yielded more favorable flow. The strength tests show that increasing nano-silica dosage proportionally increased the unconfined compressive strength (UCS) up to 2%NS, then gradually decreased. The moderate replacements (1.5%NS-2%NS) appeared better for flow issues and provided sufficient bearing strength in ground enhancement.
JOURNAL OF BUILDING ENGINEERING
(2023)
Editorial Material
Plant Sciences
Paula E. Jameson, John Clemens
JOURNAL OF EXPERIMENTAL BOTANY
(2019)
Article
Plant Sciences
Paula E. Jameson, Pragathi Dhandapani, Jiancheng Song, Marek Zatloukal, Miroslav Strnad, Mitja N. P. Remus-Emsermann, Rudolf O. Schlechter, Ondrej Novak
FRONTIERS IN PLANT SCIENCE
(2019)
Article
Agronomy
Marta Gallart, Jonathan Love, Dean F. Meason, Graham Coker, Peter W. Clinton, Jianming Xue, Paula E. Jameson, Jaroslav Klapste, Matthew H. Turnbull
Article
Multidisciplinary Sciences
Samarth, Robyn Lee, Jiancheng Song, Richard C. Macknight, Paula E. Jameson
Article
Plant Sciences
Samarth, Dave Kelly, Matthew H. Turnbull, Paula E. Jameson
Article
Forestry
Ana M. C. Teixeira, Timothy J. Curran, Paula E. Jameson, Colin D. Meurk, David A. Norton
Editorial Material
Plant Sciences
Paula Elizabeth Jameson
Article
Microbiology
Moritz Miebach, Rudolf O. Schlechter, John Clemens, Paula E. Jameson, Mitja N. P. Remus-Emsermann
Article
Plant Sciences
Matthew J. van Voorthuizen, Jaroslav Nisler, Jiancheng Song, Lukas Spichal, Paula E. Jameson
Summary: The study showed that INCYDE significantly increased seed yield in Rapid Cycling Brassica rapa, while TD-K had a transient effect on the regulation of BrCKX3.
Article
Biochemistry & Molecular Biology
Samarth, Robyn Lee, Dave Kelly, Matthew H. Turnbull, Richard C. Macknight, Anthony M. Poole, Paula E. Jameson
Summary: Research on the alpine perennial herbaceous plant Celmisia lyallii (Asteraceae) found that warm summer conditions that promote flowering lead to differential regulation of genes, including promoters and repressors of flowering. Results suggest that plants may maintain a summer memory to induce flowering.
Article
Plant Sciences
Tong Zhu, Xue Zhou, Jian-Long Zhang, Wei-Hao Zhang, Li-Pei Zhang, Chun-Xiang You, Paula E. Jameson, Peng-Tao Ma, Shan-Li Guo
Summary: Several MYB transcription factors, including MYB4-like, play vital roles in plant resistance to environmental stressors. The study revealed that NbMYB4L is involved in defense against TMV in Nicotiana benthamiana, with the ethylene pathway also playing a role. NbEIL1 acts as a positive regulator of NbMYB4L transcription in the plant, forming a coherent feed-forward loop that robustly regulates resistance to TMV.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Matthew J. van Voorthuizen, Jiancheng Song, Ondrej Novak, Paula E. Jameson
Summary: The use of plant growth regulators to enhance wheat and barley yields by altering cytokinin levels showed no significant improvement, potentially due to the inability to change yield components. Analysis of endogenous cytokinin content revealed slight changes under specific conditions.
Article
Plant Sciences
Jan E. Grant, Annu Ninan, Natalia Cripps-Guazzone, Martin Shaw, Jiancheng Song, Ivan Petrik, Ondrej Novak, Mechthild Tegeder, Paula E. Jameson
Summary: The study using transgenic peas as a model crop revealed the regulatory control over sugar and amino acid import into developing seeds and its impact on seed yield and quality. The results showed that the transgenes effectively modulated gene expression, leading to increased cytokinin levels in seed coats and promoting the transition of young leaves into source leaves. This enhanced sink activity and interactions between cytokinin and cell wall invertase ultimately resulted in higher cotyledon sucrose content and increased seed number and protein content.
FUNCTIONAL PLANT BIOLOGY
(2021)
Article
Plant Sciences
Qianqian Guo, Xia Li, Li Niu, Paula E. Jameson, Wenbin Zhou
Summary: The study emphasizes the multifaceted response of plants to drought and cold stress, highlighting physiological and genetic changes that occur during acclimation to these stresses; plants experience a decline in photosynthetic capacity under both drought and cold stress, but can recover from the stress, with internal ABA playing a key role in plant acclimation to cold.
Article
Plant Sciences
Annu S. Ninan, Jan Grant, Jiancheng Song, Paula E. Jameson