Article
Microbiology
Jun Song, Hongri Ruan, Li Chen, Yuqi Jin, Jiasan Zheng, Rui Wu, Dongbo Sun
Summary: SDQ, a specific lytic S. aureus bacteriophage, can effectively remove S. aureus biofilms. SDQ maintains its full activity under various environmental conditions and can be used in combination with detergents.
Article
Infectious Diseases
J. Martin Rodriguez, Bradford A. Woodworth, Bri'Anna Horne, Joseph Fackler, Michael J. Brownstein
Summary: This article presents a case of refractory methicillin-resistant Staphylococcus aureus that was successfully treated with a combination of antibiotics, systemic phage, and intranasal phage therapy.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Sha Liu, Karen Hon, George Spyro Bouras, Alkis James Psaltis, Keith Shearwin, Peter-John Wormald, Sarah Vreugde
Summary: The isolated Staphylococcus aureus phages showed significant bactericidal activity against the biofilm form and a broad host range, with low mutation rate, indicating potential for further development as a treatment alternative to antibiotics for Methicillin-Resistant Staphylococcus Aureus (MRSA) infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Allergy
Sha Liu, Yin Zhao, Andrew Hayes, Karen Hon, Guimin Zhang, Catherine Bennett, Hua Hu, John Finnie, Sandra Morales, Linda Shearwin, Alkis J. Psaltis, Keith Shearwin, Peter-John Wormald, Sarah Vreugde
Summary: Subinhibitory concentrations of protein synthesis inhibitor (PSI) antibiotics showed synergy when combined with phages in treating Staphylococcus aureus infections, sensitizing phage-resistant strains and promoting phage infection in vitro and in vivo. This suggests the potential use of phage-PSI antibiotic combination therapies for difficult-to-treat infections caused by phage-resistant S. aureus and MRSA strains.
Article
Immunology
Elliot Whittard, James Redfern, Guoqing Xia, Andrew Millard, Roobinidevi Ragupathy, Sladjana Malic, Mark C. Enright
Summary: This study validates the potential of phage therapy for treating Staphylococcus aureus infections by examining the characteristics and host range of lytic S. aureus phages. Phages with the widest host range and effective killing abilities against various isolates were identified, showing promise as therapeutic candidates for future studies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Virology
Hyonoo Joo, Sijia M. Wu, Isha Soni, Caroline Wang-Crocker, Tyson Matern, James Peter Beck, Catherine Loc-Carrillo
Summary: Staphylococcus aureus is the main cause of implant-related infections in the form of biofilms. The heavy use of broad-spectrum antibiotics against these pathogens contributes to the problem of antibiotic-resistant infections. Bacteriophages have the potential to be an alternative therapy.
Article
Biotechnology & Applied Microbiology
Houqi Ning, Hong Lin, Jingxue Wang, Xuebing He, Xiaoqian Lv, Lei Ju
Summary: A phage qdsa002 targeting MRSA was isolated and its endolysin Lys84 and domains were purified. Lys84 showed strong lytic activity and effectively removed MRSA biofilms, with the combination of CHAP and Amidase_2 domains exhibiting close to 90% of Lys84's activities.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Jumpei Fujiki, Tomohiro Nakamura, Keisuke Nakamura, Keita Nishida, Yurika Amano, Yusaku Watanabe, Satoshi Gondaira, Masaru Usui, Masaru Shimizu, Kazuhiko Miyanaga, Shinya Watanabe, Tomohito Iwasaki, Kotaro Kiga, Tomoko Hanawa, Hidetoshi Higuchi, Teiji Sawa, Yasunori Tanji, Yutaka Tamura, Longzhu Cui, Hidetomo Iwano
Summary: The study assessed the biological stability and clinical potential of Staphylococcus virus Phi SA012. The virus showed a wide host range and efficient lytic activity. Experiments on mice demonstrated the ability of Phi SA012 to spread and accumulate in specific organs without causing inflammation. Additionally, the study highlighted the importance of appropriate dosing and administration periods for effective phage therapy.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Fei Ma, Yu Ning, Qingqing Wan, Likou Zou, Yuntao Liu, Shujuan Chen, Jianlong Li, Zhen Zeng, Yong Yang, Hong Chen, Min Zhou, Shuliang Liu, Cheng Li, Aiping Liu
Summary: Bacteriophages LSA2308 and LSA2366 showed lytic activity against drug-resistant S. aureus, and combined with ampicillin achieved better clearance in LB broth. In skim milk, a concentration of 10^7 PFU/mL bacteriophages exhibited the most effective bactericidal efficacy against S. aureus strains.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Gohar Shaghayegh, Clare Cooksley, Mahnaz Ramezanpour, Peter-John Wormald, Alkis James Psaltis, Sarah Vreugde
Summary: This review summarizes the current understanding of inflammatory cells/pathways, the role of bacterial biofilm, particularly S. aureus biofilm, and their impact on the severity of chronic rhinosinusitis (CRS). A better understanding of these factors may provide important diagnostic and therapeutic insights for recalcitrant disease.
Review
Microbiology
Kam Pou Ha, Andrew M. Edwards
Summary: Staphylococcus aureus demonstrates remarkable adaptability and resistance in infections, with its DNA repair process playing a crucial role in pathogen survival within host tissues. Studies suggest that targeting staphylococcal DNA repair could be a potential novel therapeutic strategy.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2021)
Review
Microbiology
Sahana Vasudevan, Helma David, Lakshmi Chanemougam, Jayalakshmi Ramani, Maanasa Ramesh Sangeetha, Adline Princy Solomon
Summary: A stable but reversible phenotype switch from normal to persister state is advantageous to the intracellular pathogens to cause recurrent infections and to evade the host immune system. Staphylococcus aureus is a versatile opportunistic pathogen known to cause chronic infections with significant mortality. One of the notable features is the ability to switch to a per-sisters cell, which is found in planktonic and biofilm states. This review attempts to provide a constructed argument discussing the most widely accepted mechanisms and identifying the various attributes of persistence.
CRITICAL REVIEWS IN MICROBIOLOGY
(2022)
Article
Immunology
Dan Yang, Shuang Wang, Erchao Sun, Yibao Chen, Lin Hua, Xiangru Wang, Rui Zhou, Huanchun Chen, Zhong Peng, Bin Wu
Summary: This study isolated a temperate bacteriophage vB_Saus_PHB21 and found that its integration into the host MRSA increased the host's capacities of cell adhesion, anti-phagocytosis, and biofilm formation, leading to severe mortalities in infection.
Article
Environmental Sciences
Xiaoqiang Liu, Jingru Zuo, Jiang Teng, Le Yang, Jingjing Guo, Lianjie Liu, Pei Li
Summary: This study investigated the prevalence and multidrug-resistant (MDR) phenotype of Staphylococcus aureus in raw milk samples and farm environments. The antimicrobial and antibiofilm activities of luteolin against MDR S. aureus were also explored. The results showed that luteolin can inhibit biofilm formation, reduce cell viability, and affect gene expression related to biofilm formation in S. aureus.
ENVIRONMENTAL POLLUTION
(2023)
Article
Microbiology
Shawna McCallin, Carmen Menzi, Swenja Lassen, Jean Daraspe, Frank Oechslin, Philippe Moreillon
Summary: In Vancomycin-intermediate Staphylococcus aureus (VISA), exposure to vancomycin may lead to cross-resistance to phage. VISA strains with intermediate levels of vancomycin resistance showed decreased sensitivity to phage. Serial passaging with vancomycin induced reduced vancomycin susceptibility and phage sensitivity. Understanding the interference between antibiotic and phage resistance mechanisms is important for designing effective therapeutic regimens.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Otorhinolaryngology
Lisa Mary Cherian, Clare Cooksley, Katharina Richter, Mahnaz Ramezanpour, Sathish Paramasivan, Peter-John Wormald, Sarah Vreugde, Alkis James Psaltis
INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY
(2019)
Article
Surgery
A. S. W. Jacombs, A. Karatassas, B. Klosterhalfen, K. Richter, P. Patiniott, C. Hensman
Article
Clinical Neurology
Rajan Sundaresan Vediappan, Annika Mascarenhas, Ann Nguyen-Hoang, Stephanie Anne Fong, Alistair Jukes, Katharina Richter, Ahmed Bassiouni, Sandy Patel, Steve Chryssidis, Sophia Otto, Stephen Moratti, Alkis James Psaltis, Sarah Vreugde, Peter-John Wormald
JOURNAL OF CLINICAL NEUROSCIENCE
(2020)
Review
Multidisciplinary Sciences
Laurine Kaul, Regine Suss, Andrew Zannettino, Katharina Richter
Summary: Dithiocarbamates (DTCs) have been widely used in various applications due to their metal binding abilities and high reactivity with thiol groups. The list of potential applications for DTCs continues to grow, with approval for clinical trials against cancer, HIV, Lyme disease, and new compounds targeting diseases with unmet therapeutic needs. DTCs show promising potential as innovative medical treatments in fields such as microbiology, neurology, cardiology, and ophthalmology.
Review
Biochemistry & Molecular Biology
L. Rebecca Frkic, Katharina Richter, B. John Bruning
Summary: This review discusses the role of PPAR gamma in type 2 diabetes mellitus and the effects of synthetic ligands on the modulation of these roles. It emphasizes the critical importance of understanding the mechanism by which ligands prevent phosphorylation for future research in T2DM treatment.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Surgery
P. Patiniott, A. Jacombs, L. Kaul, H. Hu, M. Warner, B. Klosterhalfen, A. Karatassas, G. Maddern, K. Richter
Summary: This study investigated the link between bacterial biofilms and negative outcomes of hernia repair surgery. The results suggest that staphylococci biofilms may contribute to hernia repair failure, chronic pain, and exacerbation of disease.
Article
Microbiology
Laurine Kaul, Adrian I. Abdo, Tom Coenye, Bastiaan P. Krom, Michel A. Hoogenkamp, Andrew C. W. Zannettino, Regine Suess, Katharina Richter
Summary: This study describes the antibacterial properties of a combination treatment of diethyldithiocarbamate (DDC) and copper ions (Cu2+), which showed synergistic and additive effects against planktonic and biofilm cultures of Staphylococcus aureus and Staphylococcus epidermidis, with low cytotoxicity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Laurine Kaul, Clara E. Grundmann, Monika Koell-Weber, Hanna Loeffler, Artur Weiz, Andrew C. W. Zannettino, Katharina Richter, Regine Suess
Summary: This study describes the development and antibiofilm activity of an injectable gel containing a liposomal formulation of Cu(DDC)2 and Cu2+ (lipogel). The lipogel showed promising antibiofilm activity, preventing biofilm formation and killing various bacteria.
Article
Infectious Diseases
Adrian Abdo, Andrea Mcwhorter, Daniel Hasse, Thomas Schmitt-John, Katharina Richter
Summary: Plasma-treated water exhibits significant antibacterial activity, invasion inhibition, biofilm disruption, and reduction in bacterial viability against Salmonella Typhimurium, indicating its potential as an effective sanitizer for reducing Salmonella contaminations.
Article
Microbiology
Laurine Kaul, Adrian I. Abdo, Tom Coenye, Simon Swift, Andrew Zannettino, Regine Suess, Katharina Richter
Summary: This study investigates the in vitro efficacy of a Cu(DDC)2 complex and a liposomal Cu(DDC)2 + Cu2+ formulation against biofilms of Staphylococcus aureus and Staphylococcus epidermidis. The combination of DDC- and Cu2+ showed significant inhibition of biofilm formation in hernia mesh materials and wound infection models. The liposomal formulation also displayed antibacterial activity and low toxicity in an infection model using Galleria mellonella.
Review
Cell & Tissue Engineering
Anja R. Zelmer, Renjy Nelson, Katharina Richter, Gerald J. Atkins
Summary: Staphylococcus aureus, the predominant pathogen in osteomyelitis, can persist by forming intracellular reservoirs and adapting as small-colony variants (SCVs), reducing susceptibility to antibiotics. However, there are no evidence-based clinical guidelines for treating intracellular S. aureus infections in osteomyelitis. A systematic review of the literature found inconclusive results due to various methodologies employed. More standardized research is needed to improve understanding and treatment outcomes.
Article
Microbiology
Tom Coenye, Birthe Kjellerup, Paul Stoodley, Thomas Bjarnsholt
Article
Microbiology
Katharina Richter
MICROBIOLOGY AUSTRALIA
(2019)
Article
Education, Scientific Disciplines
Katharina Richter, Nicky Thomas
JOURNAL OF MICROBIOLOGY & BIOLOGY EDUCATION
(2018)