期刊
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
卷 58, 期 4, 页码 393-405出版社
KOREAN SOC HORTICULTURAL SCIENCE
DOI: 10.1007/s13580-017-0342-7
关键词
antioxidant; callus; liquid chromatography tandem mass spectrometry; microshoots; total phenolic content
类别
资金
- Deanship of Scientific Research, Yarmouk University, Jordan
Lavenders (Lavandula species) are important aromatic ornamental medicinal plants with wide ranging applications in perfume and pharmaceutical industries. We developed an in vitro propagation protocol for Lavandula coronopifolia. Murashige and Skoog (MS) medium supplemented with 0.5 mg center dot L-1 N (6) -benzyladenine was the best medium for the proliferation of microshoots, while the highest rooting frequency was obtained using MS medium supplemented with 1.0 mg center dot L-1 indole-3-butyric acid. Inter-simple sequence repeat analysis revealed that the in vitro-propagated microshoots were highly genetically stable, even after subculture. The highest callus fresh weight (667.9 mg) was obtained by propagating on medium supplemented with a combination of 1.0 mg center dot L-1 naphthaleneacetic acid and 0.5 mg center dot L-1 butyric acid. Using the Folin-Ciocalteu method, methanolic extracts of wild L. coronopifolia revealed total phenolic content of 4.9 mg expressed in gallic acid equivalents (GAE) (mg GAE center dot g(-1) dry matter). Radical scavenging activity was estimated at 85% using the free radical 2,2-diphyenyl-picrylhydrazyl assay. Using the brine shrimp assay for cytotoxicity, the methanolic extract was found to be nontoxic. Finally, liquid chromatography tandem mass spectrometry with standard reference compounds was used to quantify the key phenolic compounds in both in vitro and in vivo-grown L. coronopifolia. Six major phenolic compounds were identified: caffeic acid, rosmarinic acid, rutin, quercetin, luteolin, and hesperidin. Levels of these phenolic compounds were highest in wild plant extracts.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据