期刊
CELL REPORTS
卷 18, 期 4, 页码 857-865出版社
CELL PRESS
DOI: 10.1016/j.celrep.2016.12.078
关键词
-
类别
资金
- Swiss Government Excellence Scholarship Program
- Swiss National Science Foundation [310030_156269]
- Swiss National Science Foundation (SNF) [310030_156269] Funding Source: Swiss National Science Foundation (SNF)
T cells are the most abundant cell type found in afferent lymph, but their migration through lymphatic vessels (LVs) remains poorly understood. Performing intravital microscopy in the murine skin, we imaged T cell migration through afferent LVs in vivo. T cells entered into and actively migrated within lymphatic capillaries but were passively transported in contractile collecting vessels. Intralymphatic T cell number andmotility were increased during contact-hypersensitivity- induced inflammation and dependent on ICAM-1/LFA-1 interactions. Invitro, blockade of endothelial cell-expressed ICAM-1 reduced T cell adhesion, crawling, and transmigration across lymphatic endothelium and decreased T cell advancement from capillaries into lymphatic collectors in skin explants. In vivo, T cell migration to draining lymph nodes was significantly reduced upon ICAM-1 or LFA-1 blockade. Our findings indicate that T cell migration through LVs occurs in distinct steps and reveal a key role for ICAM-1/LFA-1 interactions in this process.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据